Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Association of growth hormone and insulin-like growth factor I genotype with body weight, dominance of body weight, and mRNA expression in Korat slow-growing chickens

  • Sinpru, Panpradub (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Bunnom, Rujjira (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Poompramun, Chotima (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Kaewsatuan, Pramin (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Sornsan, Sirangkun (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Kubota, Satoshi (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Molee, Wittawat (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Molee, Amonrat (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology)
  • Received : 2020.10.20
  • Accepted : 2021.02.27
  • Published : 2021.12.01
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Abstract

Objective: Growth hormone (GH) and insulin-like growth factor I (IGF-I) play a critical role in animal growth rates. We aimed to investigate the effect of GH and IGF-I genotypes on body weight (BW), dominance, and gene expression in slow-growing chickens at different ages. Methods: A total of 613 Korat chickens (KRs) were bred and divided into three groups by genotype - A1A1, A1A3, and A3A3 for GH and AA, AC, and CC for IGF-I. Chickens were weighed every two weeks, and liver and breast muscle tissues were collected at 10 weeks of age. Genetic parameters of KRs were estimated using ASReml software. The GH and IGF-I mRNA levels were measured by quantitative polymerase chain reaction. Significant differences between traits were analyzed using the generalized linear model. Results: A significant effect of GH genotypes on BW was found at most ages, and the A1A1 genotype had the highest value of BW. Compared with the A3A3 genotype, the A1A1 and A1A3 genotypes showed a higher dominance effect at 0 and 2 weeks, and genotype A1A1 had the highest value of dominance at 8 weeks of age. A difference in GH mRNA levels between genotypes was detected in breast muscle at 6 weeks and in the liver tissue at 2 weeks. In the case of IGF-I gene, the AA genotype had the highest BW at the beginning of life. Significant differences in BW dominance were found at 2 weeks. However, IGF-I mRNA levels were not different among genotypes in both breast muscles and liver tissues. Conclusion: Our results revealed that GH and IGF-I influence growth, but may not be involved in heterosis. GH can be used as a marker gene in selection programs for growth because the homozygous genotype (A1A1) had the highest BW at all ages. The IGF-I is not a useful marker gene for selection programs.

Keywords

Acknowledgement

The authors are grateful to the "Establishment of 'KR Chicken' Strain for Small and Micro Community Enterprise (SMCE) Production" project, which was supported by Suranaree University of Technology and Thailand Science Research and Innovation, for providing the chickens. We also thank the Center of Excellence on Technology and Innovation for Korat Chicken Business Development for their financial support, which was granted by the Suranaree University of Technology. We would like to sincerely thank Suranaree University of Technology for the Full-time Doctoral Researcher grant (Grant ID. FtR.19/2560 to A.M.).

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