• IMMUNE NETWORK
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• 제21권5호
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• pp.36.1-36.16
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• 2021

Peroxiredoxins (Prxs) are ubiquitously expressed peroxidases that reduce hydrogen peroxide or alkyl peroxide production in cells. Prxs are released from cells in response to various stress conditions, and they function as damage-associated molecular pattern molecules. However, the secretory mechanism of Prxs and their roles have not been elucidated. Thus, we aimed to determine whether inflammasome activation is a secretory mechanism of Prxs and subsequently identify the effect of the secreted Prxs on activation of the classical complement pathway. Using J774A.1, a murine macrophage cell line, we demonstrated that NLRP3 inflammasome activation induces Prx1, Prx2, Prx5, and Prx6 secretion in a caspase-1 dependent manner. Using HEK293T cells with a transfection system, we revealed that the release of Prx1 and Prx2 relies on gasdermin-D (GSDMD)-mediated secretion. Next, we confirmed the binding of both Prx1 and Prx2 to C1q; however, only Prx2 could induce the C1q-mediated classical complement pathway activation. Collectively, our results suggest that inflammasome activation is a secretory mechanism of Prxs and that GSDMD is a mediator of their secretion. Moreover, secreted Prx1 and Prx2 bind with C1q, but only Prx2 mediates the classical complement pathway activation.

• 한국자원식물학회지
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• 제37권3호
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• pp.256-262
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• 2024

Blueberry (BB), fruit of Vacciniumi, has been hailed as an antioxidant superfood. BB is a rich source of vitamins, minerals, flavonoids, phenolic acids and known to have a variety of pharmacological actions. The purpose of this work is to clarify the anti-inflammatory mechanism of BB in lipopolysaccharide (LPS)-activated RAW264.7 macrophage. We explored the effects of BB on the production of inflammatory cytokines, prostaglandin E₂ (PGE₂) and expression of cyclooxygenase (COX)-2 in LPS-activated RAW264.7 macrophage. Moreover, to investigate the molecular mechanisms by BB, we evaluated whether BB modulate nuclear factor-kappa B (NF)-kB pathway and caspase- 1 activation. The findings of this work demonstrated that BB alleviated the LPS-enhanced inflammatory cytokines and PGE₂, as well as COX-2 levels. Additionally, we demonstrated that the anti-inflammatory mechanism of BB occurs due to the attenuation of IκB-α degradation, NF-kB translocation and caspase-1 activation. Conclusively, these findings provide evidence that BB may be useful agents in the treatment of inflammation.

• 한국결정학회지
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• 제16권2호
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• pp.66-74
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• 2005

다양한 생리활성을 가지는 marine natural products는 일반적인 secondary metabolites와 유사한 구조를 가지는데, 염소, 브롬, 요오드의 할로겐 원소에 의해 수식이 되어있는 것이 일반적이다. Vanadium haloperoxidase는 이러한 marine natural products의 생산에 중요한 효소로 vanadate를 조효소로 하는 금속효소이다. 본 리뷰에서는 vanadium haloperoxidase의 분리와 단백질 구조를 살펴보고, 이 금속효소의 작용기작에 대해서 설명할 것이다. 마지막으로, vanadium haloperoxidase의 반응성과 secondary metabolites 중 indole, terpenoids, acetogenins의 생합성 예를 살펴볼 것이다.

• The Korean Journal of Physiology and Pharmacology
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• 제24권1호
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• pp.1-10
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• 2020

Autophagy is a highly conserved intracellular degradation and energy-recycling mechanism that contributes to the maintenance of cellular homeostasis. Extensive researches over the past decades have defined the role of autophagy innate immune cells. In this review, we describe the current state of knowledge regarding the role of autophagy in neutrophil biology and a picture of molecular mechanism underlying autophagy in neutrophils. Neutrophils are professional phagocytes that comprise the first line of defense against pathogen. Autophagy machineries are highly conserved in neutrophils. Autophagy is not only involved in generalized function of neutrophils such as differentiation in bone marrow but also plays crucial role effector functions of neutrophils such as granule formation, degranulation, neutrophil extracellular traps release, cytokine production, bactericidal activity and controlling inflammation. This review outlines the current understanding of autophagy in neutrophils and provides insight towards identification of novel therapeutics targeting autophagy in neutrophils.

• Advances in Traditional Medicine
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• 제10권1호
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• pp.37-43
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• 2010

Palmitic acid (PA) is one of free fatty acids, which is found from Gaultheria itoana Hayata and Sarcopyramis nepalensis. Although PA has a variety of pharmacological effects including mediates hypothalamic insulin resistance, induces IP-10 expression, and promote apoptotic activities, the anti-inflammatory mechanism of PA in mouse peritoneal macrophages remains unclear. In this study, we showed that PA exerted an anti-inflammatory action through suppression the production of tumor necrosis factor-$\alpha$, interleukin-6, cyclooxygenases-2 and nitric oxide in lipopolysaccaride-stimulated mouse peritoneal macrophages. Our study suggests an important molecular mechanism of PA, which might explain its beneficial effect in the regulation of inflammatory reactions.

• 한국기계가공학회지
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• 제8권2호
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• pp.18-24
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• 2009

The most dominate aspect in machine works using CNC devices in industrial production processes is the precision of the product and the Cycle Time. To this day, many studies on the external factors of the technology to reduce the Cycle Time have advanced amid to the advancements in cam soft development for manual programs and the numerous studies on high speed and precision machining. This study experimented various functions of the sequence pattern flow and arranged system development technologies of past few years to develop and applicate various usage of adjustment factors within the CNC, so it would be more understandable to the user and would enable them to make high speed and precision products more faster develop and. In order to reduce the Cycle Time, the mechanism of machine tools has to be analysed and applied, in addition to program reduction and improvement of the manufacture process.

• 한국정밀공학회지
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• 제25권10호
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• pp.92-98
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• 2008

This paper is concerned with the designing and making power standing wheelchair. This wheelchair is studied based on the mechanical and electrical engineering concepts and theories. The mechanical theories are composed of statics and dynamics knowledges that are related with moving and standing position. Basically the static and dynamic stability is the most important element in designing and making the real size model. The linear actuator is used in the standing mechanism and the joystick controlled by hand is attached on the arm rest. The real size model is made and also investigated through the design specifications by test drive. Finally, this paper proves the possibility of commerical production of power standing wheelchair.

• Journal of Microbiology
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• 제42권3호
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• pp.200-204
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• 2004

Retron is a prokaryotic genetic element that produces multicopy single-stranded DNA covalently linked to RNA (msDNA) by a reverse transcriptase. It was found that cells producing a large amount of msDNA, rather than those that did not, showed a higher rate of mutation. In order to understand the molecular mechanism connecting msDNA production to the high mutation rate the protein patterns were compared by two dimensional gel electrophoresis. Ten proteins were found to be differentially expressed at levels more than three fold greater in cells with than without msDNA, nine of which were identified by MALDI TOF MS. Eight of the nine identified proteins were repressed in msDNA-producing cells and, surprisingly, most were proteins functioning in the dissimilation of various carbon sources. One protein was induced four fold greater in the msDNA producing cells and was identified as a 30S ribosomal protein S2 involved in the regulation of translation. The molecular mechanism underlying the elevated mutation in msDNA-producing cell still remains elusive.

• Industrial Engineering and Management Systems
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• 제8권4호
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• pp.213-220
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• 2009

A revenue sharing contract is one of the mechanisms that coordinate decision makers in a decentralized supply chain toward the consensual goal. The transfer prices between different echelons in the supply chain influence the total supply chain profits. The study aims to explore various transfer pricing heuristics on the supply chain coordination in terms of the supply chain profits and their interactions with the revenue sharing rate. A model is proposed for formulating the collaborative production and distribution planning in a decentralized supply chain with the revenue sharing mechanism. Experiment results indicate that the transfer price and the revenue sharing rate affect significantly the coordination. Among the studied pricing heuristics, the variable-cost pricing method led to the best SC profits. Raising the revenue sharing rate reduced the SC profits no matter what heuristics were employed. Furthermore, the experiments provide us clues for finding the optimal transfer price for the supply chain.

• 한국분말재료학회지
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• 제4권4호
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• pp.275-282
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• 1997

Sintered oilless bearing using fluid-dynamic mechanism for high revolution of over 10,000 rpm was developed by Powder metallurgy Process. Developed bearing was composed of Fe-40-60%Cu of which inner face consisted of six-regular-prominence-and-depression. Inner face has controlled pore size and amount according to application. Jitter and friction properties were tested under assembled conditions with housing and motor. Bearing-own properties and precision were also tested because of demanding for assembled properties. Measurement-skill as well as mould-production-skill were investigated for the precision of bearing. Final precision reached 2 ${\mu}m$ in inner diameter tolerance and 5 ${\mu}m$ in coaxiality under assembled conditions.