• Parasites, Hosts and Diseases
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• v.31 no.3
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• pp.285-292
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• 1993

C. sinensis total RMh was containing large amount of 185 rRNA but little 285 rRNA. The size of the double-stranded cDNA synthesized from poly $(A)^{+}$ mRNA was 0.4-4.2 kb long with tapering unto 9.5 kb. Degenerated oligonucleotides (as 2 sense and 3 antisense Primers) were designed on the conserved regions of the known tropomyosin amino acid sequences. From one out of the PCR amplifications using total CDNA and matrix of primers, a specific gene product, 580 bp in size, was produced. Upon Southern hybridization of the PCR products with Schistosomn mnnsoni tropomyosin (SMTM) CDNA, only one signal appeared at the band of 580 bp product. This 580 bp product was considered to encode C. sinensis tropomyosin (CSTM) and cloned in pGEM-3Zf(-) for DNA sequencing. CSTM cDNA was 575 bp containing one open reading frame of 191 predicted amino acids, which revealed 86.3% homology with SMTM and 51.1% with rrichostronsylur coeubnlormis tropomyosin. CSTM cDNA obtained will serve as a probe in the studies of molecular cloning of CSTM.

• Journal of the Korean Institute of Telematics and Electronics
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• v.26 no.11
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• pp.1880-1885
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• 1989

In this paper, a new approach for systolic array realizing the discrete cosine transform (DCT) based on discrete Fourier transform (DFT) of an input sequence is presented. The proposed array is based on a simple modified DFT(MDFT) version of the Goertzel algorithm combined with Kung's approach and is proved perfectly. This array requires N cells, one multiplier and takes N clock cycles to produce a complete N-point DCT and also is able to process a continuous stream of data sequences. We have analyzed the output signal-to-noise ratio(SNR) and designed the circuit level layout of one-PE chip. The array coefficients are static adn thus stored-product ROM's can be used in place of multipliers to limit cost as eliminate errors due to coefficients quantization.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1996.03b
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• pp.130-137
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• 1996

Several cold extrusion processes to produce an axisymmetric steel piston-pin are investigated for comparing each other. Two methods among four conventinal ones are selected to be simulated using the rigid-plastic finite element method. One of the both methods using a mechanical press has one stage process and the other utilizing a cold header applies a multi-stage process to produce a final product. Because the main process is a backward extrusion, the design criteria such as the backward extrusion ration and punch diameter to depth rationare ocnsidered. FEM analysis is performed mainly for strain distributin and load-stroke relationshis. Based on the results of preliminary simulatin, both process sequences are proved to have proper charicteristics suitable for each production method in terms of maximum load. Those simulation results will be a good design criteria in the future work to advance the manufacturing process.

• Journal of Korean Institute of Industrial Engineers
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• v.29 no.1
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• pp.49-64
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• 2003

Although assembly sequence planning is an essential task in assembly process planning, it is known as one of the most difficult and time consuming jobs because its complexity is increased geometrically when the number of parts in an assembly is increased. The purpose of this study is to develop a more efficient algorithm for generating assembly sequences automatically. By considering subassemblies, a new heuristic method generates a preferred parallel assembly sequence that can be used in robotic assembly systems. A parallel assembly sequence concept provides a new representation scheme for an assembly in which the assembly sequence precedence information is not required. After an user inputs both the directional mating relation information and the mating condition information, an assembly product is divided into subgroups if the product has cut-vertices. Then, a virtual disassembly process is executed to generate alternate parallel assembly sequences with intermediate assembly stability. Through searching parts relations in the virtual disassembly process, stable subassemblies are extracted from translation-free parts along disassembling directions and this extraction continues until no more subassemblies are existed. Also, the arithmetic mean parallelism formula as a preference criterion is adapted to select the best parallel assembly sequence among others. Finally a preferred parallel assembly sequence is converted to an assembly BOM structure. The results from this study can be utilized for developing CAAPP(Computer-Aided Assembly Process Planning) systems as an efficient assembly sequence planning algorithm.

• Journal of the Korean Society of Clothing and Textiles
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• v.27 no.9_10
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• pp.1160-1171
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• 2003

This study is intended to examine the current situation and the problems of the "worksheets, regarding various factors that exert influences onto the quality-risk management, which is one of the most significant factors specifying an apparel product. This paper advances according to the following sequences: 1. Literature survey 2. Field research on actual "apparel vendors" and production factories 3. Investigating the requirements relating to the worksheet 4. Developing standard format of the worksheet 5. Effectiveness analysis on the standard worksheet 6. Proposal of an intelligent model applying standard worksheet system. This study is performed with the following research methods; those are questionnaire surveys, statistical analysis, interviews, collecting & analyzing worksheets, product sample quality experiment, functional analysis of the system, and materialization of the prototype system. The significance of this research is that it suggests the possible effective use of worksheet, which reflects the customers' needs in terms of the product quality, at the stage of the product planning process. Despite its importance, the current work structure does not visibly reflect these components. Therefore, with the result of this research, it is considered that the worksheet systems will meet more useful improvements in risk management process. The limitations of this research are as follows. Although it is evident that the standard worksheets bring effective results in quality improvements of products, considerations must be made onto some additional payoffs arising from the additional cost of necessary labor forces with expert knowledge and cost of the effort and time inputting correct information for running the system.

• Journal of the Korean Society for Precision Engineering
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• v.11 no.4
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• pp.77-87
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• 1994

This paper deals with an automated forming sequence design system by which designers can determine desirable operation sequences even if they have little experience in the design of cold forging process. The forming sequence design in the cold forging is very important and requires many kinds of technical and empirical knowledge. They system isproposed, which generates forming sequence plans for the multistage cold forging of axisymmtrical solid products. Since the process of metal forming can be considered as a transformation of geometry, treatment of the geometry of the product is a key in planning process. To recognize the geometry of the product section, section entity representation and primitive geometries were used. Section entity representation can be used for the calculation of maximum diameter, maximum height, and volume. Forming sequence for the part can be determined by means of primitive geometries such as cylinder, cone, convex, and concave. By utilizing this geometrical characteristics (diameter, height, and radius), the product geometry is expressed by a list of the priitive geometries. Accordingly the forming sequence design is formulated as the search problem which starts with a billet geometry and finishes with a given product one. Using the developed system, the sequence drawing with all dimensions, which includes the proper sequence of operations for the part, is generated under the environment of AutoCAD. Based on the results of forming sequence, process variables(strain, punch pressure, die inner pressure, and forming load) are determined.

• Journal of fish pathology
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• v.23 no.3
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• pp.313-322
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• 2010

Edible tunicate Halocynthia roretzi, one of the most commercially important aquatic organisms in Korea, has been killed by tunic softness syndrome since last decade. The intracellular protistan parasite observed by the transmission electron microscope in hemocytes of the tunicate was considered to be the causative agent of the mass mortality. The goal of the present work is to examine the characteristic features of the parasite by identifying the 18S rDNA sequences of the parasite. The experiments conducted include amplification of presumptive 18S rDNA from diseased tunicate tissues with UNonMet-PCR and sequencing the product. A preliminary phylogenetic analysis was performed on the presumptive parasite rDNA. A digoxigenin labeled DNA probe was designed on the basis of the sequences of rDNA. Dig-ISH assay was conducted to diagnose the protistan parasite. A PCR using UNonMet-PCR primer generated 595 bp SSU rDNA fragment. Subsequently, PCRs with primer pair expended this sequence to 1542 bp. This is the first partial sequences of SSU rDNA gene to be published on the protistan parasite that has presumed causing the mass mortality of tunicate. Since the Dig-ISH technique demonstrated the presence of infection in hemocytes on the all host tissues, the fragment was confirmed to be the intracellular protistan parasite SSU rDNA. A phylogenetic analysis suggested that the protistan parasite may be a unique eukaryote that is closely related to Apicomplexa.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.91-97
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• 1999

In this work, a 1.9-kb region of enterococcal plasmid p703/5 was isolated and the nucleotide sequence analysis of the region was performed. One major open reading frame (ORF) was identified encoding a polypeptide of 28 kDa. Database comparisons suggested that the protein showed some homology with other bacterial RepA proteins. Upstream of the ORF, a potential dnaA box, AT-rich region and 22-bp tandemly repeated sequences (DNA iterons), a feature typical for many replication ori sites, were recognized. Deletion analysis using Exonuclease III and several restriction enzymes indicated that the three elements and the gene product from the ORF were essential for replication and that the minimum unit of DNA required for replication resided on the 1.2-kb AvaII subfragment. Thus, this gene product was referred to as RepA.

• Journal of Microbiology and Biotechnology
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• v.4 no.4
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• pp.256-263
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• 1994

The aceB gene, encoding for malate synthase, one of the key enzymes of glyoxylate bypass, was isolated from a pMT1-based Corynebacterium glutamicum gene library via complementation of an Escherichia coli aceB mutant on an acetate minimal medium. The aceB gene was closely linked to aceA, separated by 598 base pairs, and transcribed in divergent direction. The aceB expressed a protein product of Mr 83, 000 in Corynebacterium glutamicum which was unusually large compared with those of other malate synthases. A DNA-sequence analysis of the cloned DNA identified an open-reading frame of 2, 217 base pairs which encodes a protein with the molecular weight of 82, 311 comprising 739 aminoo acids. The putative protein product showed only limited amino acid-sequence homology to its counteliparts in other organisms. The N-terminal region of the protein, which shows no apparent homology with the known sequences of other malate synthases, appeared to be responsible for the protein s unusually large size. A potential calciumbinding domain of EF-hand structure found among eukaryotes was detected in the N-terminal region of the deduced protein.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.43 no.2
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• pp.137-145
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• 2020

Various elements of Fabrication (FAB), mass production of existing products, new product development and process improvement evaluation might increase the complexity of production process when products are produced at the same time. As a result, complex production operation makes it difficult to predict production capacity of facilities. In this environment, production forecasting is the basic information used for production plan, preventive maintenance, yield management, and new product development. In this paper, we tried to develop a multiple linear regression analysis model in order to improve the existing production capacity forecasting method, which is to estimate production capacity by using a simple trend analysis during short time periods. Specifically, we defined overall equipment effectiveness of facility as a performance measure to represent production capacity. Then, we considered the production capacities of interrelated facilities in the FAB production process during past several weeks as independent regression variables in order to reflect the impact of facility maintenance cycles and production sequences. By applying variable selection methods and selecting only some significant variables, we developed a multiple linear regression forecasting model. Through a numerical experiment, we showed the superiority of the proposed method by obtaining the mean residual error of 3.98%, and improving the previous one by 7.9%.