• Journal of Climate Change Research
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• v.9 no.1
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• pp.1-12
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• 2018

There is an emphasis on the importance of adaptation against to climate change and related natural disasters. As a result, various climate information with different time-scale can be used for science-based climate change adaptation policy. From the aspects of Global Framework for Climate Services (GFCS), various time-scaled climate information in Korea is mainly produced by Korea Meteorological Administration (KMA) However, application of weather and climate information in different application sectors has been done individually in the fields of agriculture and water resources mostly based-on weather information. Furthermore, utilization of climate information including seasonal forecast and climate change projections are insufficient. Therefore, establishment of the Cooperation Center for Application of Weather and Climate Information is necessary as an institutional platform for the UIP (User Interface Platform) focusing on multi-model ensemble (MME) based climate service, seamless climate service, and climate service based on multidisciplinary approach. In addition, APCC Integrated Modeling Solution (AIMS) was developed as a technical platform for UIP focusing on user-centered downscaling of various time-scaled climate information, application of downscaled data into impact assessment modeling in various sectors, and finally producing information can be used in decision making procedures. AIMS is expected to be helpful for the increase of adaptation capacity against climate change in developing countries and Korea through the voluntary participation of producer and user groups within in the institutional and technical platform suggested.

• The Korean Journal of Archival Studies
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• no.59
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• pp.321-353
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• 2019

As the function and role of the Record Center that manage the processing department, which is the producer of records, are very passive and limited, there are growing demands for expansion of work and organization along with change in this. In order to strengthen the function and role of the Record Center, it is necessary to enrich the substance through identification of the value of preservation and utilization of records under the Record Center, development of various tasks, and the construction of independent Record Center, etc. in addition to complementing institutional devices. In this paper, we examine the construction background and process, the remaining tasks of Record Center in Ministry of Justice, and intend to find out what matters and procedures the central administrative agency should consider when establishing the Record Center based on relevant case study.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2046-2056
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• 2018

Phospholipase D has great commercial value due to its transphosphatidylation products that can be used in the food and medicine industries. In order to construct a strain for use in the production of PLD, we employed a series of combinatorial strategies to increase PLD expression in Bacillus subtilis WB600. These strategies included screening of signal peptides, selection of different plasmids, and optimization of the sequences of the ribosome-binding site (RBS) and the spacer region. We found that using the signal peptide amyE results in the highest extracellular PLD activity (11.3 U/ml) and in a PLD expression level 5.27-fold higher than when the endogenous signal peptide is used. Furthermore, the strain harboring the recombinant expression plasmid pMA0911-PLD-amyE-his produced PLD with activity enhanced by 69.03% (19.1 U/ml). We then used the online tool \RBS Calculator v2.0 to optimize the sequences of the RBS and the spacer. Using the optimized sequences resulted in an increase in the enzyme activity by about 26.7% (24.2 U/ml). In addition, we found through a transfer experiment that the retention rate of the recombinant plasmid after 5 generations was still 100%. The final product, phosphatidylserine (PS), was successfully detected, with transphosphatidylation selectivity at 74.6%. This is similar to the values for the original producer.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.220-225
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• 2019

Studies based on microbial community analyses have increased in the recent decade since the development of next generation sequencing technology. Associations of gut microbiota with host's health are one of the major outcomes of microbial ecology filed. The major approach for microbial community analysis includes the sequencing of variable regions of 16S rRNA genes, which does not provide the information of bacterial activities. Here, we conducted RNA-based microbial community analysis and compared results obtained from DNA- and its cDNA-based microbial community analyses. Our results indicated that these two approaches differed in the ratio of Firmicutes and Bacteroidetes, known as an obesity indicator, as well as abundance of some key bacteria in gut metabolisms such as butyrate producers and probiotics strains. Therefore, cDNA-based microbial community may provide different insights regarding roles of gut microbiota compared to the previous studies where DNA-based microbial community analyses were performed.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.258-267
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• 2019

The purpose of this study was to investigate the effect of lactic acid bacteria (LAB) on the growth and biogenic amines (BA) formation of Enterobacter aerogenes CIH05 in skim milk and soymilk. Lactobacillus acidophilus GK20, Lactobacillus paracasei GK74, and Lactobacillus plantarum GK81 isolated from mustard kimchi did not produce BA in the decarboxylation broth. L. paracasei GK74 exhibited the highest cell viability and antimicrobial compounds producing ability in fermented skim milk and soymilk samples, while the lowest producer was L. plantarum GK81. The production yield of lactic acid, hydrogen peroxide, and bacteriocin was dependent on the species of Lactobacillus and the type of culture medium. As LAB the number of viable cells of E. aerogenes CIH05 were higher in skim milk than in soymilk. When mixed culture with L. acidophilus GK20 and L. paracasei GK74 and treated with bacteriocin solution (300 AU/ml) obtained from these strains in milk media, the cell growth and cadaverine and histamine contents of E. aerogenes CIH05 were significantly (P < 0.05) lower than the respective values in control sample.

• Korean Journal of Environmental Agriculture
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• v.38 no.3
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• pp.159-165
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• 2019

BACKGROUND: The water footprint (WF) is an indicator of freshwater use that appears not only at direct water use of a consumer or producer, but also at the indirect water use. As an indicator of 'water use', the water footprint includes the green, blue, and grey WF, and differs from the classical measure of 'water withdrawal' because of green and grey WF. This study was conducted to assess and estimate the water footprint of the soybean and Chinese cabbage. METHODS AND RESULTS: APEX model with weather data, soil and water quality data from NAS (National Institute of Agricultural Sciences), and farming data from RDA (Rural Development Administration) was operated for analyzing the WF of the crops. As the result of comparing the yield estimated from APEX with the yield extracted from statistic data of each county, the coefficients of determination were 0.83 for soybean and 0.97 for Chinese cabbage and p-value was statistically significant. The WFs of the soybean and Chinese cabbage at production procedure were 1,985 L/Kg and 58 L/Kg, respectively. This difference may have originated from the cultivation duration. The WF ratios of soybean were 91.1% for green WF and 8.9% for grey WF, but the WF ratios of Chinese cabbage were 41.5% for green WF and 58.5% for grey WF. CONCLUSION: These results mean that the efficiency of water use for soybean is better than that for Chinese cabbage. The results could also be useful as an information to assess environmental impact of water use and agricultural farming on soybean and Chinese cabbage.

• Journal of Marine Bioscience and Biotechnology
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• v.11 no.1
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• pp.14-22
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• 2019

Omega-3 fatty acids and carotenoids, which are known as representative high-value substances derived from microalgae, are being studied from various diatoms. Most of the diatoms contain fucoxanthin and omega-3 fatty acid. Fucoxanthin produced by diatom has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. However, the low growth rate and fucoxanthin content of diatoms are one of the big obstacles to the industrial application. In this study, indigenous marine diatom Achnanthidium sp. BS-001 was isolated for a candidate of fucoxanthin producer. Light intensity and temperature for the culture of Achnanthidium sp. BS-001 were optimized on PhotoBiobox. Optimization of silicate concentration for increasing BS-001 biomass productivity was confirmed in F/2 medium with various concentration of sodium silicate. As a result, condition of light intensity, temperature, and silicate concentration for optimal cultivation were $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $18^{\circ}C$ and 0.106 mM, respectively. Maximum biomass productivity reaches to $154.3mg{\cdot}L^{-1}{\cdot}day^{-1}$, and then the content of omega-3 fatty acids and fucoxanthin were $19.4mg{\cdot}g^{-1}$, $9.05mg{\cdot}g^{-1}$, respectively. These results indicate that Achnanthidium sp. BS-001 has the potential to be used as a source of omega-3 fatty acids and fucoxanthin.

• The Korean Journal of Archival Studies
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• no.41
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• pp.75-111
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• 2014

With the quantitative growth of research data, the issue of enormous preservation cost and sharing expansion, the organizations should prioritize the collections then select the data that are worthy of save. Therefore, today, it is important for the organizations to appraise the continuing value of produced records. Considering the universities and the public institutions such as governmentfunded research institutes as the heavy producer of the data, it becomes a rising problem for the records management that it does not go beyond the framework of "administrative records" and "public records". In this study, I looked into the background of the contention that the research records must be managed in a different perspective and checked the characteristics of research records in therms of the producers, research activities and records. Based on this analysis, I suggested the main issues and considerations about the subjects, criterias and methods in research records appraisal.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.29 no.1
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• pp.108-118
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• 2019

Objective: The purpose of this study was to investigate the effects of occupational stress factors on the level of stress and depression of entertainment program producers and entertainment program writers. Methods: This study was based on analysis of a survey collected by 65 respondents out of 224 questionnaires who are entertainment program PDs and entertainment program writers. The survey was conducted from December 20, 2017 to February 20, 2018. Results: In the logistic regression analysis conducted to investigate the factors affecting the stress level in the job-related characteristics of a) higher total career, b) shorter working period in the current firm, c) longer weekly working hours and d) more count of weekend work, the results found higher stress levels. In the sub-factors of job stress, the stress level encountered by respondents was significantly higher for those with a) higher job demand, b) lower insufficient job control and c) higher job instability. In the logistic regression analysis conducted to investigate the factors affecting depression, the depression level in entertainment PD was higher than the entertainment writer in the sociodemographic characteristics. In the sub-factors of job stress, the stress level was significantly higher for those with higher job demand, lower insufficient job control, and higher job instability. For job-related characteristics, depression was significantly higher for longer weekly working hours. Conclusions: Entertainment program producers and entertainment program writers suffer from psychosocial stress and depression which are caused by excessive job demands, lack of job autonomy and job instabilities. Those factors must be managed and also their workweek should be shortened.

• Korean Journal of Veterinary Service
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• v.42 no.2
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• pp.67-71
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• 2019

A variety of livestock feed resources were used in Korean dairy farm due to a lack of the endemic feed. However, there is inadequate real farm data to support farmers' decisions on the choice of options. The main objective of this study was to evaluate the nutritional value of total mixed ration (TMR) as well as the metabolic diseases status in Korean dairy farms. TMR samples were collected from nine feed companies and eight selected self-formulated by the dairy farms. The nutrient contents were examined by AOAC methods. The frequency of metabolic diseases such as ketosis and hypocalcemia were surveyed. The average moisture content was 36.2% although the min. and max. value were varied from 21.7% and 50.6% among farms. The mean${\pm}$standard deviation of crude fiber (CF), crude ash (CA), ether extract (EE), and crude protein (CP) were $21.4{\pm}2.5$, $4.6{\pm}0.4$, $3.2{\pm}0.5$ and $9.8{\pm}1.7$, respectively. However, the average ADF and NDF was $17.3{\pm}3.7$ and $31.0{\pm}5.7$, respectively. The compositions of TMR were varied significantly among the dairy farms. The frequency of clinical Ketosis (CK), subclinical ketosis (SCK) and hypocalcemia were higher in early lactation period with 4.5%, 11.0% and 3.0%, respectively. Also, the frequency of SCK was higher than CK and hypocalcemia throughout the lactation. Periodic TMR nutrient analysis based on herd production or physiology change would maximize the effects of TMR feeding. Furthermore, the study results would be useful to the farm practitioner and producer for their farm management.