• Proceedings of the Microbiological Society of Korea Conference
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• 2001.11a
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• pp.146-156
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• 2001

The biosynthetic gene clusters for bluensomycin and spectinomycin were isolated and characterized from the bluensomycin producer, Streptomyces bluensis ATCC27420 and the spectinomycin producer, Streptomyces spectabilis ATCC27741, respectively. PCR primers were designed specifically to amplify a segment of dTDP-glucose synthase gene based on its conserved sequences of several actinomycete strains. By screening cosmid libraries using amplified PCR fragments, 30-kb and 45-kb DNA fragments were isolated from Streptomyces bluensis and Streptomyces spectabilis, respectively. Sequencing analysis of them revealed that each contains 15 open reading frames (ORFs). Some of these ORFs were turned out to be antibiotic resistance genes (blmA and speN), dTDP-glucose synthase genes (blmD and spcD), and dTDP-D-glucose 4,6-dehydratase genes (blmE and spcE), suggesting that the blm and spec gene clusters are likely involved in the biosynthesis of bluensomycin and spectinomycin, respectively.

• Journal of the Korea Fashion and Costume Design Association
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• v.7 no.2
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• pp.1-10
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• 2005

This study intends to examine extensively on the current situation where the full capacity of 3D CG is not being highlighted because of the lack of the information and the awareness and to look at how the virtual reality technology is being applied ranging from the design of the clothes to the marketing. A set of processes ranging from the development of the clothing design to the marketing will be performed on the web. Designers will design with 3D CG and make the patterns and will hand this over to the producer together with the virtual swatch. But there are important problems to work out. First, it is the problem of the virtual fitting room. Second, it is the absence of the 3D CG, which is easy and convenient for the clothing design. Third, it is to perfect the visual reconstruction. Fourth, it is the security of the distribution system. Fifth, it also calls for the strengthened internet network that can smooth the flow of the tremendous data. Consumer will be able to produce according to their needs and will become designer and producer at the same time, resulting in the achievement of the consumer-oriented marketing in real sense.

• Journal of the Korean Society of Costume
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• v.60 no.8
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• pp.51-66
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• 2010

This study aimed to analyze the changing characteristics between Men's Gender Role and Men's wear Design in 20th century. To analyze the representative Men' Gender roles, the related studies and literatures reviewed. Literature reviews and quantitative studies were performed to analyze the changing differences of Korean Men's wear Deign as a symbolic factors of gender role. The results of study are as follows. 1) The representative men's gender roles in 1900's~1920's were 'Patriarchal/ Stern Man'. The narrow V-zone of jacket and shoulder, high shirt collars and well fitted suits expressed the stern images of that times. 2) In 1930's~50's, 'Enervated/ Escapist Man' were the representative images. They were expressed with wide lapels and shoulders, emphasized V-zone and abundant trousers. Especially 40's style was under the influence of American 'Zoot Style'. 3) The representative gender roles were the men of 'Producer/Provider' in 60's~70's. Shortened jacket, wide lapels & neckties, slim trousers were used to express the strong & young images of Producer/ Provider during the industrialization era. 4) The representative gender roles were 'Attentive/ Intellectual/ Beautiful Men' in 80's~90's. The exaggerated round shoulders which were influenced by 'Big Look', and easy silhouette were related with the attentive images.

• Microbiology and Biotechnology Letters
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• v.25 no.6
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• pp.580-585
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• 1997

We selected two mutants namely strain D5 and Nu23 by mutagenesis of anthracycline producing Streptomyces: the former is an $\varepsilon$-rhodomycinone overproducing mutant selected from Streptomyces sp. C5, a baumycin producer and the latter, a blocked mutant of early pathway for doxorubicin biosynthesis obtained from Streptomyces peucetius ATCC 27952, a doxorubicin producer. The mutant strain Nu23 does not produce anthracycline metabolites but retains the most of enzyme activities converting aklavinone to doxorubicin and the mutant strain D5 produced $\varepsilon$-rhodomycinone at a level of 150 $\mu$g/ml. These strains were grown separately in NDYE medium and each was mixed at day 3 by equal volume of culture broth but the quantity of doxorubicin produced was far below an estimation based on the level of $\varepsilon$-rhodomycinone normaly produced by the strain D5. On the other hand doxorubicin was reached at maximum level after 4 days in the mixed culture condition which was composed of culture broth of strain D5 grown for 6 day and that of strain Nu23 grown for 3 day. It was turned out that the growth of mutant strain D5 was inhibited by the accumulation of daunorubicin and doxorubicin in mixed culture broth, which cause the limitation of $\varepsilon$-rhodomycinone.

• Journal of the Korea Furniture Society
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• v.26 no.3
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• pp.267-273
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• 2015

This study is a review on the relationship between furniture purchasers and producers in the Joseon period. Understanding the relationships in our traditional spirit and the Joseon furniture that reflects a aesthetic sense is a meaningful work. Joseon was to build an ideal country which put emphasis on the order and harmony through Theory of 'Yeak' as the confucianism nation. Human aesthetics, which emphasis on the spiritual value by confirmity to nature and forcus on the relaxed, deficient personality rather than completeness was established as the central formative consciousness of the Joseon Dynasty. The nature of things in the confucian scholar prior to the representations of things is expressed in the form of master craftsman's skill and soul. Formative consciousness of the confucian scholar is to realize ideals by communciation and interchange with master craftsman's soul in the form of the Joseon furniture. The beauty of restraint and brevity in the Joseon Dynasty furniture includes meanings and procedures of art work. It implies an orderer's purpose of production and a producer's products. Understanding the spirit of times in the production becomes the cornerstone capable of getting closer to the furniture ultimate proper function. These problems have a very close relationship with the problems of modern people's commnuication. We expect an even higher growth of the furniture by achieving more improved communication based on the consumer's great insight and the producer's passion.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1120-1124
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• 2005

Pseudomonas putida KCTC 1639 was newly identified as a potential producer of biodegradable medium chain length polyhydroxyalkanoates. It exhibited a carbon assimilation pattern quite different from other known P. putida strains, but a more similar pattern with P. oleovorans, which assimilates the carbon sources mainly through ${\beta}$-oxidation rather than the fatty acid biosynthesis pathway. The PHA synthesis gene cluster from P. putida KCTC1639 was composed of two gene loci; the PHA synthase gene locus and granule-associated gene locus, which were cloned and deposited in the GenBank under accession numbers AY286491 and AY750858 as a new nucleotide sequence, respectively. The molecular structure and amino acid homology of the new gene cluster were compared with those from Pseudomonas species, including other P. putida strains and P. oleovorans, and a higher than $90\%$ homology was observed.

• Microbiology and Biotechnology Letters
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• v.27 no.6
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• pp.433-439
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• 1999

Among microorganisms isolated from soil, YJ-66 strain was the best producer of flocculant and was examined for flocculating ability in the active carbon and CaCl2. YJ-66 strain was the best producer of flocculant and was examined for flocculating ability in the active carbon and CaCl2. YJ-66 strain was identified to be a species belonging to the genus Achromobacter. The optimum culture condition for production of bioflocculant with the isolated strain was for 72hrs at 3$0^{\circ}C$ and pH7.5. The favorable carbon, nitrogen sources and inorganic salts for production of the flocculant were sucrose, peptone, MgSO4 and KH2PO4, whose optimal concentrations were 2%. 0.067%, 0.1% and 0.1%, respectively. Addition of the carbon and inorganic salts significantly increased the production of flocculant. Compositions of optimized culture medium for bioflocculant production by Achromobacter sp. YJ-66 were 2% sucrose, 0.067% peptone, 0.1% MgSO4 and 0.1% KH2PO4 in initial pH 7.5 during at 3$0^{\circ}C$ for 72hrs.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1787-1795
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• 2015

The transition from primary to secondary metabolism in antibiotic-producing Streptomyces correlates with expression of genes involved in stress responses. Consequently, regulatory pathways that regulate specific stress responses are potential targets to manipulate to increase antibiotic titers. In this study, genes encoding key proteins involved in regulation of the osmotic stress response in Streptomyces avermitilis, the industrial producer of avermectins, are investigated as targets. Disruption of either osaB_Sa, encoding a response regulator protein, or osaC_Sa, encoding a multidomain regulator of the alternative sigma factor SigB, led to increased production of both oligomycin, by up to 200%, and avermectin, by up to 37%. The mutations also conditionally affected morphological development; under osmotic stress, the mutants were unable to erect an aerial mycelium. In addition, we demonstrate the delivery of DNA into a streptomycete using biolistics. The data reveal that information on stress regulatory responses can be integrated in rational strain improvement to improve yields of bioactive secondary metabolites.

• Proceedings of the Korea Contents Association Conference
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• 2006.05a
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• pp.255-258
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• 2006

TV drama is the entertainment content which is the very popular and profitable program than any other TV program genre. Although recently some broadcasting companies have been tried to set up the drama production system, they don't have the formal system yet. This study analyses the practices and mechanisms of the drama producing process by doing the dept-interview with the experts in the drama production field. The Korean drama production system is 'the producer-director system' and the strategies for the windowing effects are not applied systematically from the pre-production step. For the broadcasting companies to have the competitiveness through drama, the expert-producer system should be applied, the production elements should be controled systematically, and the multi-use strategies should be established.

• Journal of Korean Society of Rural Planning
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• v.21 no.3
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• pp.33-45
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• 2015

The oversea export of agricultural-product about item and quantity has not increased recently; especially the fresh-product has a tough issue because of period of production, price large fluctuations, customs clearance, quarantine, and uncertainty about actual locality, we need the information based construction to exchange information quickly about whole range of export and to focus capacity of participation subject for increasing the export. In this study we design the agricultural-product transaction information system based on crowdsourcing to transact the agricultural-product and the information of influencing benefit directly, and the information offering about export-procedure from participation of customs clearance, finance, distribution, buyer, and producer's guild, etc. We expect the producer's guild about agriculture that has not participate the trade to be able to export the agricultural-product and the stabilization of price to transact the product of collapsed or boomed through the agricultural-product information system based on crowdsourcing.