• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.987-996
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• 2009

Aspergillus niger GH1 previously isolated and identified by our group as a wild tannase producer was grown under solid-state (SSC) and submerged culture (SmC) conditions to select the enzyme production system. For tannase purification, extracellular tannase was produced under SSC using polyurethane foam as the inert support. Tannase was purified to apparent homogeneity by ultrafiltration, anion-exchange chromatography, and gel filtration that led to a purified enzyme with a specific activity of 238.14 IU/mg protein with a final yield of 0.3% and a purification fold of 46. Three bands were found on the SDS-PAG with molecular masses of 50, 75, and 100 kDa. PI of 3.5 and 7.1% N-glycosylation were noted. Temperature and pH optima were 600e and 6.0 [methyl 3,4,5-trihydroxybenzoate (MTB) as substrate], respectively. Tannase was found with a $K_M$ value of $0.41{\times}10^{-4}M$ and the value of $V_{max}$ was $11.03{\mu}$moL/min at $60^{\circ}C$ for MTB. Effects of several metal salts, solvents, surfactants, and typical enzyme inhibitors on tannase activity were evaluated to establish the novelty of the enzyme. Finally, the tannase from A. niger GH1 was significantly inhibited by PMSF (phenylmethylsulfonyl fluoride), and therefore, it is possible to consider the presence of a serine or cysteine residue in the catalytic site.

• Journal of Microbiology and Biotechnology
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• v.13 no.5
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• pp.759-766
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• 2003

Recombinant Chinese hamster ovary (rCHO) cell lines expressing a high level of human thrombopoietin (hTPO) or its analog, TPO33r, were obtained by transfecting expression vectors into dihydrofolate reductase-deficient (dhfr) CHO cells and subsequent gene amplification in media containing stepwise increments in methotrexate (MTX) level such as 20, 80, and 320 nM. The parental clones with a hTPO expression level $>0.40\;{\mu}g/ml$ (27 out of 1,200 clones) and the parental clones with a TPO33r expression level $>0.20\;{\mu}g/ml$ (36 out of 400 clones) were subjected to 20 nM MTX. The clones that displayed an increased expression level at 20 nM MTX were subjected to stepwise increasing levels of MTX such as 80 and 320 nM. When subjected to 320 nM MTX, most clones did not display an increased expression level, since the detrimental effect of gene amplification on growth reduction outweighed its beneficial effect of specific TPO productivity ($q_{TPO}$) enhancement at 320 nM MTX. Accordingly, the highest producer subclones ($1-434-80^{*}$ for hTPO and $2-3-80^{*}$ for TPO33r), whose $q_{TPO}$ was 2- to 3-fold higher than that of their parental clones selected at 80 nM MTX, were isolated by limiting dilution method and were established as rCHO cel1 lines. The $q_{TPO}$ of $1-434-80^{*}\;and\;2-3-80^{*}\;was\;5.89{\pm}074\;and\;1.02{\pm}0.23\;{\mu}g/10^6$ cells/day, respectively. Southern and Northern blot analyses showed that the enhanced $q_{TPO}$ of established rCHO cell lines resulted mainly from the increased TPO gene copy number and subsequent increased TPO mRNA level. The hTPO and TPO33r produced from the established rCHO cell lines were biologically active in vivo, as demonstrated by their ability to elevate platelet counts in treated mice.

• Microbiology and Biotechnology Letters
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• v.48 no.3
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• pp.328-336
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• 2020

Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.

• Microbiology and Biotechnology Letters
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• v.48 no.3
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• pp.287-295
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• 2020

Lactobacillus zymae GU240 was previously isolated from Kimchi, a Korean fermented vegetable, as a strong GABA producer. The strain showed β-galactosidase (β-Gal) activity on MRS agar plates with X-gal. When growth and β-Gal activities of GU240 were measured using MRS (glucose, 2%, w/v) and MRSL (lactose, 2%, w/v) broths, cells were found to grow slowly in MRSL, and the β-Gal activity (36 units at 4 h) was lower than that of cells grown in MRS (94 units at 16 h). The highest OD₆₀₀ value of the culture in MRS was 1.6 at 24 h at 37℃, whereas that of the culture in MRSL was 0.6 at 16 h. β-Gal activity of the culture in MRS reached the maximum (95.6 u/ml) at 16 h, decreased thereafter, and was not detected at 48 h. β-Gal activity for culture in MRSL reached its highest (36 u/ml) at 4 h and decreased gradually, but some activity (11.05 u/ml) still remained at 72 h. The structural gene encoding β-Gal in L. zymae GU240 was cloned as a 3.1 kb fragment, and DNA sequencing confirmed the presence of complete lacLM genes. lacLM genes from L. zymae GU240 showed 98-99% homologies in nucleotide sequences with other lacLM genes from L. brevis. Reverse transcription (RT)-PCR confirmed the operon structure of lacLM. The results indicated that L. zymae GU240 might be in the process of losing the ability to grow rapidly on lactose-containing medium, such as milk, due to adaptations to plant environments, including kimchi.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.1
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• pp.1-9
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• 2013

Long term hydrogen production was investigated in an anaerobic sequencing batch reactor (ASBR) using mixed microflora. Glucose (about 8,250 mg/L) was used as a substrate for the ASBR operation under the condition of pH 5.5 and $37^{\circ}C$ with mixing at 150 rpm. The experiment was carried out over a period of 160 days. Hydrogen yield was 0.8mol $H_2/mol$ glucose with F/M ratio 2 at initial operation period. The hydrogen yield reached to maximum 2.6 mol $H_2/mol$ glucose at 80th day operation. However decreased hydrogen yield was observed after 80 days operation and eventually no hydrogen yield. Although well-known hydrogen producer Clostridium sp. was detected in the reactor by PCR-DGGE analysis, changed reactor operation was the major reason of the decreased hydrogen production, such as low F/M ratio of 0.5 and high propionic acid concentration 2,130 mg/L. Consequently the long period operation resulted in MLSS accumulation and then low F/M ration stimulating propionic acid formation which consumes hydrogen produced in the reactor.

• The Journal of the Korea Contents Association
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• v.17 no.1
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• pp.701-717
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• 2017

Criticism is related with recognition activity which is interpreting and evaluating the text based on the particular criteria. It reflects the awareness system of a particular time-period in that it is a work of social meanings beyond individual impressions. However, the standards of criticism have changed, like the awareness system has changed as economy, culture or technology has evolved. The emergence of SNS has influenced the field of popular culture. It improves the role and participation of the public in criticism. Moreover, it breaks the previous paradigm of production and consumption in cultural industry. These changes bring not only the positive ones, such as improvement of diversity and freedom of criticism, but also the negative one, confusion of values due to the absence of criteria. This study mainly concerned debates and discourses of SNS on , a new song of IU, and focused on changes of aesthetic evaluations of culture performer, producer and audience. Especially, the authors tried to find out how such new technology has changed the criticism, and how it has influenced on our aesthetic consciousness.

• The Journal of the Korea Contents Association
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• v.18 no.10
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• pp.78-98
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• 2018

On the 855 pure Korean commercial fictional movies, excluding diversity films, released in Korea from 2004 to August 2017, I conducted deciles distribution analysis of box office performance of those movies and average box office performance of directors, producers and lead actors who involved in making them. Deciles distribution analysis of average box office performance might be helpful to predict their next box office performance of newly produced Korean movies and to evaluate their contribution to box office performance. In baseball, the various index such as winning rate, on-base percentage, slugging percentage, stolen base percentage, battling average, earned run average is used for predicting and reviewing of professional players. In this study, I evaluate the script's narrative quality by the indirect method of insight and judgment of creative manpower involved in making the movies. For the more productive prediction, direct statistical analysis method on the narrative of the script needs to develop. Time series analysis is required to evaluate the rise and fall of creative manpower and network analysis is also necessary to see the interaction among creative people.

• Resources Recycling
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• v.22 no.2
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• pp.29-36
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• 2013

Although Korean domestic production of flat panel displays totalled more than 48 trillion KRW in 2007, most of the flat panel display wastes have been land-filled or incinerated, which greatly overshadows Korean national prestige as a world leading producer and developer of flat panel display devices. Countries such as Japan or EU possess quite limited land-fill capability and have sought ways to dispose of WEEEs from environment-friendly perspective rather than recovery of valuable materials from the wastes. Considering relatively short cycle of about 5 years for flat panel display devices, it is estimated that more than 5 million units will be accumulated as wastes by 2015. Urban mining is a most suitable countermeasures against China's monopoly of rare and rare earth metals, which are contained in flat panel display wastes. Therefore, materials recycling of waste LCD units has to be developed and commercialized soon enough for economic and environment-friendly recovery of valuable resources hidden in LCD wastes.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.231-238
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• 2018

This study was carried out to develope new zoysiagrass cultivars 'JangsungChorok' (Application No. :2013-3) and 'JangsungSaetbyeol' (Application No. :2013-4). One hundred and one genetic resources were collected at Jangsung Gun area from 2010 to 2011. Among the collected lines, CY6097 (JangsungChorok) and CY6069 (JangsungSaetbyeol) showed superior performance in spring green up and growth rate. 'JangsungChorok' showed genetically light green leaf color, with medium leaf width (4.4 mm), height from ground to first leaf blade of 3.2 cm and average plant height of 18 cm. Fast recovery rate and early spring green up rate will make this cultivar (JangsungChorok) for efficient sod producer. 'JangsungSaetbyeol' showed genetically dark green leaf color, with fine to medium leaf width of 3.5 mm, height from ground to first leaf blade of 4.3 cm and average plant height of 22.6 cm. Fast recovery rate and early spring green up rate with relatively narrow leaf will make this cultivar (JangsungSaetbyeol) for high sod quality.

• Journal of the Korean Wood Science and Technology
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• v.41 no.3
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• pp.258-268
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• 2013

Wood-based products market is new growth engine industry of "Green growth policy". Korean government established a law, sustainable use of wood, for expanding wood and wood-based products market in 2012. This study was conducted to seek sustainable measures for conservation and management of valuable natural resources, offer expanding quality assurance services for wood-based products, and explore measures for expanding wood-based products labeling system. Through discussed domestic and overseas trends and literatures that concern wood-based products labeling system and investigated case studies that were presently operational. For expanding quality assurance services of wood-based products, Korea Forest Service operates a wood-based products labeling system in integrated management through variety awareness and specialist assessment for each product. This process will make enhanced wood-based products labeling system expanded gradually. To evolve into $3^{rd}$ party quality assurance services on maturity age of labeling system, public communication will help improve awareness of producer and consumer. appropriate incentive program will attract voluntary participation from woodbased product industry. Producers consider environmental ethics in their business and consumer should exercise their right to know.