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Production of ${\alpha}2-Macroglobulin$ by a T Cell Hybridoma (T 세포 하이브리도마에 의한 ${\alpha}2-Macroglobulin$의 생산)

  • Lee, Chong-Kil;Han, Seong-Sun
    • YAKHAK HOEJI
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    • v.38 no.6
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    • pp.715-720
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    • 1994
  • ${\alpha}2-macroglobulin$ $({\alpha}2-M)$ has been shown to have a variety of activities. One of those activities is the suppression of immune response. Characterization of the immunosuppressive factor secreted by a T cell hybridoma showed that ${\alpha}2-M$ was produced and secreted from the T cell hybridoma. ${\alpha}2-M$ was produced abundantly from the T cell hybridoma when cultured as ascites. The isolation and identification of the ${\alpha}2-M$ were studied using affinity chromatography and N-terminal amino acid sequencing. The extended observations were that the ${\alpha}2-M$ produced by the T cell hybridoma suppresses mixed lymphocyte reaction.

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The measurement and analysis of the electric field waveforms produced by lightning discharges (뇌방전에 의하여 발생하는 전계파형의 측정과 분석)

  • Lee, B.H.;Ju, M.N.;Kil, G.S.;Ahn, C.H.
    • Proceedings of the KIEE Conference
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    • 1995.11a
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    • pp.444-446
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    • 1995
  • This paper deals with semisphere-type sensor fo measuring the electric field waveforms by lightning discharges. The theoretical principle and design rule of the device are introduced, and also the calibration and application investigations are carried out. From the calibration experiments, the frequency bandwidth of the semisphere-type electric field measuring device ranges from 200 [Hz] to 1.56 [MHz], and the sensitivity of sensor is 0.96 [mV/V/m]. The electric field waveforms produced by lightning discharges were observed for June and August 1995. It is shown that the electric field waveforms produced at the distance of more than 50 [km] include only radiation component.

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Production and Characterization of Chitosan from Ginseng-Steaming Effluents by Mucor miehei

  • Kim, Jae-Ho;Lee, Ki-Sung;Kim, Na-Mi;Lee, Jong-Soo
    • Journal of Microbiology and Biotechnology
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    • v.12 no.5
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    • pp.760-765
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    • 2002
  • Mucor miehei KCTC 6011, which grew successfully in ginseng-steaming effluents and produced a large amount of chitosan efficiently, was selected from various fungi. Approximately 120 mg of chitosan per g-dry mycelium was maximally produced in 84 h at $25^{\circ}C$ when grown in the ginseng-steaming effluent (pH 8.0) supplemented with 0.5% yeast extract and 0.002% CuSO$_4$. Chitosan produced by Mucor miehei KCTC 6011 was identified by the IR-spectra to have deacety lated approximately 56%. Viscosity and molecular weight of the chitosan were 80 cps and $1.07\times10^3$ kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rhizoctonia solani in 60 h.

Non-Destructive Detection of Hertzian Contact Damage in Ceramics

  • Ahn, H.S.;Jahanmir, S.
    • Tribology and Lubricants
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    • v.11 no.5
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    • pp.114-121
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    • 1995
  • An ultrasonic technique using normal-incident compressional waves was used to evaluate the surface and subsurface damage in ceramics produced by Hertzian indentation. Damage was produced by a blunt indenter (tungsten carbide ball) in glass-ceramic, green glass and silicon nitride. The damage was classified into two types; (1) Hertzian cone crack, in green glass and fine grain silicon nitride, and (2) distributed subsurface micro fractures, without surface damage, produced in glass ceramic. The ultrasonic technique was successful in detecting cone craks. The measurement results with the Hertzian cone cracks indicated that cracks perpendicular to the surface could be detected by the normal-incident compressional waws. Also shown is the capability of normal-incident compressional waves in detection distributed micro-sized cracks size of subsurface microfractures.

The Reversible Contraction on Relaxation of Isolated Rat Aorta (흰쥐의 대동맥 이완반응에 대한 재수축효과)

  • 김진학;신창열;박조영;민영실;최경범;염지현;이남인;김학림;손의동
    • Biomolecules & Therapeutics
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    • v.8 no.2
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    • pp.113-118
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    • 2000
  • TEA, glibenclamide, L-NAME and SKF 525A-induced reversible contraction were investigated using acetylcholine, sodium nitroprusside (SNP) and pinacidil in rat abdominal and thoracic aorta. Acetylcho-line, SNP or pinacidil produced in a dose dependent manner relaxation on phenylephrine-induced contraction In rat aorta. TEA, SKF 525A, and L-NAME produced reversible contractions on acetylcholine-induced relaxation, but not on SNP- or pinacidil-induced relaxation. Glibenclamide significantly produced reversible con- traction on pinacidll-induced relaxation. The reversible effect of TEA on the acetylcholine-induced relaxation was reduced by SKF 525A. These results indicate that the acetylcholine-induced relaxation may be mediated by NO, cytochrome P$_{450}$-dependent epoxygenase pathway, or $Ca^{2+}$ activated $K^{+}$ channel, and the pinacidil-induced relaxation may be mediated by ATP-sensitive $K^{+}$ channel.annel.

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Electrochemical Regeneration of FAD by Catalytic Electrode Without Electron Mediator and Biochemical Reducing Power

  • JEON SUNG JIN;SHIN IN HO;SANG BYUNG IN;PARK DOO HYUN
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.281-286
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    • 2005
  • We created a new graphite-Cu(II) electrode and found that the electrode could catalyze FADH$_2$ oxidation and FAD reduction coupled to electricity production and consumption, respectively. In a fuel cell with graphite-Cu(II) anode and graphite-Fe(III) cathode, the electricity was produced by coupling to the spontaneous oxidation of FADH$_2$ Fumarate and xylose were not produced from the enzymatic oxidation of succinate and xylitol without FAD, respectively, but produced with FAD. The production of fumarate and xylose in the reactor with FAD electrochemically regenerated was maximally 2- 5 times higher than that in the reactor with FAD. By using this new electrode with catalytic function, a bioelectrocatalysts can be engineered; namely, oxidoreductase (e.g., lactate dehydrogenase) and FAD can function for biotransformation without an electron mediator and second oxidoreductase for cofactors recycling.

A Study on Strength Characteristic as the Fineness Modulus and Curing Method of Oyster shells (굴 패각의 조립률 및 양생방법에 따른 강도특성에 관한 연구)

  • Jung, Ui-In;Hong, Sang-Hun;You, Nam-Gyu;Song, Seung-Li;Kim, Bong-Joo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2018.05a
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    • pp.62-63
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    • 2018
  • Oyster shell is produce by shucking process in oyster farming in southern coast of Korea. In average, about 6.7kg of oyster shell is produced as an industrial waste for 1kg of oyster flesh, and even only in last year, it is estimated that about 150,000 ton of oyster shell is produced. Oyster shell is light weighted and the strength characteristic of it is similar to send. So we produced mortar test piece using grounded oyster shell according to aggregate and reviewed strength characteristic. Therefore, in this study, the strength characteristics of the test specimen are evaluated by artificially altering fineness modulus and curing method by processing oyster shells.

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Statistical Analysis of Electric Field Waveforms Produced by Lightning Return Stroke (낙뢰에 의해서 발생되는 전장파형의 통계적 분석)

  • Lee, B.H.;Park, S.Y.;Ahn, C.H.;Kil, K.S.
    • Proceedings of the KIEE Conference
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    • 1997.07e
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    • pp.1824-1826
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    • 1997
  • In this paper, in order to obtain statistical informations on lightning electromagnetic waveforms, electric field waveforms produced by lightning return strokes were measured and analyzed. The electric field measuring system consists of hemisphere antenna 30[cm] in diameter, integrator and data acquisition. system. The frequency bandwidth of the measuring system is 200[Hz] to 1.56[MHz], and the sensitivity is 0.96[mV/V/m]. The mean value of front time of electric field waveforms produced by positive lightning return strokes is 5.87[${\mu}s$], and that of negative is 4.12[${\mu}s$]. The mean values of zero-crossing time for positive or negative electric field waveforms are 35.00 and 26.61[${\mu}s$], respectively. The mean value of percentage dip-depth for positive electric field waveforms is 33.68[%], and that for negative is 28.36[%].

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Characterization of Soot Produced from Thermal Decomposition of Hydrocarbon Fuel (탄화수소연료의 열분해 Soot발생특성 분석에 관한 연구)

  • KIM, HAKDUCK;KIM, CHANGYEAN;SONG, JUHUN
    • Transactions of the Korean hydrogen and new energy society
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    • v.27 no.6
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    • pp.747-752
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    • 2016
  • Soot and harmful exhaust gas produced by liquid hydrocarbon fuel caused various environmental problems. In this study, soot characteristics produced by thermal decomposition of acetylene and diesel were analyzed, which are formed at different temperatures. These fuels were observed to produce particulate matters, and collected soot samples were observed by using TEM & SEM. These were found to be significantly different in structure and crystallinity.

A Comparative Study of Intonation Phrase Boundary Tones of Korean Produced by Korean Speakers and Chinese Speakers in the Reading of Korean Text (중국인 학습자들의 한국어 억양구 경계톤 실현 양상)

  • Yune, Young-Sook
    • Phonetics and Speech Sciences
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    • v.2 no.4
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    • pp.39-49
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    • 2010
  • The purpose of this paper is to examine how Chinese speakers realize Korean intonation phrase (IP) boundary tones in the reading of a Korean text. Korean IP boundary tones play various roles in speech communication. They indicate prosodic constituents' boundaries while simultaneously performing pragmatic and grammatical functions. In order to express and understand Korean utterances correctly, it is necessary to understand the Korean IP boundary tone system. To investigate the IP boundary tone produced by Chinese speakers, we have specifically examined the type of boundary tones, the degree of internal pitch modulation of boundary tones, and the pitch difference between penultimate syllables and boundary tones. The results of each analysis were compared to the IP boundary tones produced by Korean native speakers. The results show that IP boundary tones were realized higher than penultimate syllables.

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