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Inhibition of Listeria monocytogenes by Bacteriocin(s) from Lactic Acid Bacteria Isolated from Kimchi (김치에서 분리한 젖산균 bacteriocin에 의한 Listeria monocytogenes의 억제)

  • Kim, Jung-Ho
    • Applied Biological Chemistry
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    • v.38 no.4
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    • pp.302-307
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    • 1995
  • Four strains of lactic acid bacteria which produced bacteriocins inhibitory to Listeria species were isolated from Kimchi, and were identified as Leuconostoc mesenteroides subsp. mesenteroides (2 strains), Leuconostoc paramesenteroides and Pediococcus pentosaceus. The bacteriocins produced by the isolates inhibited all of the Listeria monocytogenes strains tested, but L. denigrificans 28 and L. welchimeri 89 were not inhibited by the bacteriocin produced by the Leu. paramesenteroides isolate. The bacteriocin produced by the P. pentosaceus isolate was more inhibitory against sensitive strains and showed broader spectrum of antimicrobial activity than those produced by other isolates. The bacteriocins produced by Leuconostoc isolates were sensitive to pronase E treatment, but that produced by the P. pentosaceus isolate was not completely inactivated. The bacteriocins produced by all of the isolates were not sensitive to catalase, ${\alpha}$-amylase and lysozyme and heat (30 min at $100^{\circ}C$) treatments.

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Compositional Differences of Bojungikgi-tang and Yukmijihwang-tang Decoctions Produced by the Extractors Made of Various Materials (전탕 용기에 따른 보중익기탕과 육미지황탕 전탕액 비교 연구)

  • Kim, Jung-Hoon;Lee, Nari;Shin, Hyeun-Kyoo;Seo, Chang-Seob
    • Korean Journal of Pharmacognosy
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    • v.45 no.3
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    • pp.249-255
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    • 2014
  • This study compared Bojungikgi-tang (BIT) and Yukmijihwang-tang (YJT) decoctions produced using the extractors made of various materials, such as glass, earthenware, and stainless steel, for 60, 90, and 120 min. The extraction yield was measured and the amounts of marker compounds in decoctions were determined by quantitative analysis using high performance liquid chromatography. The extraction yields of BIT decoctions produced by three extractors were not significantly different, whereas the extraction yield of YJT produced using glass extractor was significantly higher than that of YJT produced using earthenware extractor after 90 min. The amounts of most marker compounds were highest in BIT decoctions produced using glass extractor. However, variations of the amounts of marker compounds were observed in YJT decoctions and the volatile compound was not easily vaporized in YJT decoction produced using earthenware extractor. This study suggests that the extractor made of various materials may affect the extraction efficiency and the contents of marker compounds, especially in the decoction produced using earthenware extractor, although the results were dependent on the characteristics of herbal formula.

Accuracy of casts produced from conventional and digital workflows: A qualitative and quantitative analyses

  • Abduo, Jaafar
    • The Journal of Advanced Prosthodontics
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    • v.11 no.2
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    • pp.138-146
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    • 2019
  • PURPOSE. Comparing the accuracy of casts produced from digital workflow to that of casts produced from conventional techniques. MATERIALS AND METHODS. Whole arch alginate (ALG) and polyvinyl siloxane (PVS) impressions were taken with stock trays and custom trays, respectively. The ALG impressions were poured with type III dental stone, while the PVS impressions were poured with type IV dental stone. For the digital workflow, IOS impressions were taken and physical casts were produced by 3D printing. In addition, 3D printed casts were produced from images obtained from a laboratory scanner (LS). For each technique, a total of 10 casts were produced. The accuracies of the whole arch and separated teeth were virtually quantified. RESULTS. Whole arch cast accuracy was more superior for PVS followed by LS, ALG, and IOS. The PVS and ALG groups were inferior in the areas more susceptible to impression material distortion, such as fossae and undercut regions. The LS casts appeared to have generalized errors of minor magnitude influencing primarily the posterior teeth. The IOS casts were considerably more affected at the posterior region. On the contrary, the IOS and LS casts were more superior for single tooth accuracy followed by PVS and ALG. CONCLUSION. For whole arch accuracy, casts produced from IOS were inferior to those produced from PVS and ALG. The inferior outcome of IOS appears to be related to the span of scanning. For single tooth accuracy, IOS showed superior accuracy compared to conventional impressions.

A Study on the Pitch Contour Variation in Reading Sentence Produced by Chinese Korean-Learners (중국인 학습자들의 한국어 낭독 문장 피치곡선의 변동 양상)

  • Yune, Youngsook
    • Phonetics and Speech Sciences
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    • v.5 no.2
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    • pp.59-69
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    • 2013
  • The purpose of this study is to examine the variation of pitch contour observed in the reading of Korean sentences produced by Chinese Korean-learners. In the reading context, Korean sentence intonation can be described by considering accentual phrases' pitch pattern and intonational phrases' boundary tone. But when APs and IPs connect to each other to form sentences, another aspect of speech production must be considered, that is declination of pitch contour. So, in order to examine how Chinese speakers produce Korean sentence intonation, we have analysed the sentences' pitch contours produced by fourteen Chinese speakers differing in proficiency, and compared them to pitch contours produced by six Korean native speakers. The results show that Chinese speakers tend to decline the pitch contour in shorter sentences, but for longer sentences, the declination was not observed. Moreover, even though Chinese speakers produced sentences with declination, internal tonal modulation differs from native speakers.

A Study on the Detection and the Correction of Prosodic Errors Produced by Chinese Korean-Learners (중국인 학습자들의 한국어 강세구 실현양상과 오류진단 및 교정방안 연구)

  • Yune, Young-Sook
    • Phonetics and Speech Sciences
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    • v.4 no.2
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    • pp.51-59
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    • 2012
  • The purpose of this study is to examine the pitch pattern of Korean accentual phrases produced by Chinese Korean-learners in the reading of a Korean text. Korean accentual phrase is determined by a specific F0 contour. And the pitch contour of APs differ depending on their length and the nature of initial segment. In order to examine if Chinese speakers are also aware such a phonetic properties, we have examined the AP pitch contours produced by 15 Chinese speakers differing in proficiency, and compared them to pitch contours produced by six Korean native speakers. The results show that Chinese speakers' pitch errors were observed in initial segment-tone interaction and in type of pitch patterns. However, even though Chines speakers produced the same type of pitch patterns, internal tonal modulation differs from native speakers. Finally, on the basis of theses results, we proposed a teaching method that visualizes the F0 contour.

Survey and Control of the Occurrence of Mycotoxins from Postharvest Cereals (수확 후 곡류에 발생하는 진균독소의 캄색과 방제 1. 옥수수, 밀에서 분리한 Penicillium이 생산하는 주요 진균독소)

  • 오소영;정일민;백수봉;유승헌
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.700-704
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    • 1998
  • A total of 26 and 55 isolates of fungi were isolated from corn and wheat samples collected from different markets in Korea, respectively. The number of Penicillium isolates from corn and wheat was 9 and 33, respectively. The Penicillium species isolated from corn were P. chrysogenum (3 isolates) and P. oxalicum (6 isolates), and from wheat were P. aurantiogriseum (16 isolates), P. citrinum (1 isolate), P. commun (4 isolates), P. griseofulvum (1 isolate), P. verrucosum (7 isolates), and P. viridicatum (4 isolates). Production of major mycotoxins in the yeast extract sucrose medium cultures of Penicillium isolates was analysed. Penicillium cultures were extracted with chloroform and purified by thin-layer chromatograhy (TLC), and high performance liquid chromatography (HPLC). Among 9 isolates of Penicillium from corn, 2 isolates of P. chrysogenum produced patulin, 1 isolate of the fungus produced patulin and citrinin, 2 isolates of P. oxalicum produced penicillic acid, 4 isolates produced pencillic acid and griseofulvin. Of the 33 isolates of Penicillium from wheat, 6 isolates of P. aurantiogriseum produced patulin, 8 isolates produced penicillic acid, 1 isolate produced patulin and penicillic acid, 1 isolate of P. citrinum produced citrinin and patulin, 2 isolates of P. commun produced brefeldin A and patulin, 1 isolate of P. griseofulvum produced brefeldin A, griseofulvin and patulin. Five isolates of P. verrucosum produced patulin, 1 isolate of the fungus produced penicillic acid, and 3 isolates of P. viridicatium produced penicillic acid.

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An Acoustical Study of English Diphthongs Produced by American Males and Females (미국인 남성과 여성이 발음한 영어이중모음의 음향적 연구)

  • Yang, Byung-Gon
    • Phonetics and Speech Sciences
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    • v.2 no.2
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    • pp.43-50
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    • 2010
  • English vowels can be divided into monophthongs and diphthongs depending on the number of vocal tract shapes. Diphthongs are usually produced with more than one shape. This study attempts to collect acoustical data of English diphthongs published by Hillenbrand et al.(1995) online and to examine acoustic features of the diphthongs for phoneticians and English teachers. Sixty three American males and females were chosen after excluding those subjects with different target vowels or ambiguous formant tracks. The author used Praat to obtain the acoustical data systematically at eleven equidistant timepoints over the diphthongal segment. Obvious errors were corrected based on the spectrographic display of each diphthong. Results show that the formant trajectories of the diphthongs produced by the American males and females appeared quite similar. When the female formant values were uniformly normalized to those of the males, almost a perfect collapse occurred. Secondly, the diphthongal movements on the vowel space appeared not linear due to the coarticulatory gesture for the following consonant. Thirdly, the average duration of the diphthongs produced by the females was 1.156 times longer than that of the males while the pitch ratio between the two groups turned out to be 1.746 with a similar contour over measurement points. The author concludes that English diphthongs produced by various groups can be compared systematically when the acoustical values are obtained at proportional timepoints. Further studies will be desirable on the comparison of English diphthongs produced by native and nonnative speakers.

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Evaluation on the Field Application of Spontaneous Acoustic Field Reproduction System (능동형 음장조정시스템의 현장적용 평가)

  • Jeon, Ji-Hyeon;Shin, Yong-Gyu;Kang, Sang-Woo;Min, Byeong-Cheol;Kook, Chan
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2006.11a
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    • pp.616-621
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    • 2006
  • A began of this study is to verify Spontaneous Acoustic Field Reproduction System(SAFRS), developed as an embodiment of creating agreeable sound environment, with evaluation on the field application. SAFRS is a system to sense changes of surroundings and produce sounds, which can go well with environment elements sensed by the system in to the space. The sound which can go well with environment elements is sound which judged by individual evaluation to be so, the classification of the preferred sounds according to the mood of the space was suggested in the former study. So, SAFRS was applied into the Square of D University to evaluate effectiveness of the system. The executed evaluations were 1) evaluation on sounds perception, frequency, volume and matchability with the space, 2) image evaluation on the square and sound environment and 3) evaluation on sound environment with existing sounds, fountains sound, sound produced by SAFRS, and both fountains sound and sound produced by SAFRS. Verifying SAPRS of field application was deduced from those evaluations. Theresultsofthestudyarefollowing: Though the system was applied into the space, the volume of the sounds shouldn't be too high. And with visual surroundings, the effectiveness of the system would be increased. At the results of four evaluations, the result of day evaluation is; both fountains sound and sound produced by SAFRS>fountains sound>sound produced by SAFRS>existing sounds, the result of night evaluation is; sound produced by SAFRS>both fountains sound and sound produced by SAFRS>fountains sound>existing sounds and these results pointed out that sounds environment produced by the system was highly evaluated due to less background sounds.

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Fatigue Life Prediction of Welded Structural Material under Variable Loading (변동하중(變動荷重)을 받는 용접구조재(熔接構造材)의 피로수명(疲勞壽命) 예측(豫測))

  • Kim, Min-Gun;Kim, Dong-Yul
    • Journal of Industrial Technology
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    • v.18
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    • pp.187-193
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    • 1998
  • In this study, about the fatigue life of welded structure material under fluctuation loading, the prediction life which is produced by using the Histogram Recorder System was compared with the experimental life which is produced by the RMC model which is imported by conception of equivalent stress. In this result, this is represented few difference by comparing prediction life which is produced by damage analysis depended on Miner's rule, by using the Histogram Recorder System, with experimental life which is produced by the RMC load model which is imported by conception of equivalent of stress, therefore fatigue life is easily predicted by using Histogram Recorder System, and result of prediction has equivalent accuracy with other method which is more complex than the Histogram Recorder System. Besides the damage which is produced by stress which is high thirty percentage rank in the stress range of damage inducing, is nearly equal to the damage which is induced the rest of seventy percentage, there fore we can see that damage accumulation which is induced few time overload which is effected welded structure material is great.

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An Analysis of the English l Sound Produced by Korean Students

  • Yang, Byung-Gon
    • Speech Sciences
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    • v.15 no.1
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    • pp.53-62
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    • 2008
  • The purpose of this study was to examine the English l sound in an English short story produced by 16 Korean students in order to determine various allophones of the sound using acoustic visual displays and perceptual judgments. The subjects read the story in a quiet office at normal speed. Each word included the lateral sound in onset or coda positions and before a vowel of the following word. Results showed as follows: Firstly, there was a durational difference between the two major groups. Also the majority of the subjects produced the clear l regardless of the contexts. Some students produced the sound as the Korean flap or the English glide [r]. A few missing cases were also seen. The dark l was mostly produced by the subjects of English majors in coda position with a few cases before a vowel in a phrase. Visual displays using the computer analysis were very helpful in distinguishing lateral variants but sometimes perceptual process would be necessary to judge them in fast and weak production of the target word. Further studies would be desirable to test the discrepancies between the acoustical and perceptual decisions.

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