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Comparison of Infrequent Restriction Site-Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis for Molecular Typing of Staphylococcus aureus and Escherichia coli (황색포도구균과 대장균의 기준형별 결정에 있어서 Infrequent Restriction Site Polymerase Chain Reaction과 Pulsed-Field Gel Electrophoresis의 변별력 비교)

  • Shin, Wan-Shik;Kim, Tai-Gye;Choi, Jung-Hyun;Lee, Dong-Gun;Choi, Hee-Baeg;Yoo, Jin-Hong;Kim, Jong-Hyun;Kang, Jin-Han;Min, Woo-Sung
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.4
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    • pp.289-297
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    • 2000
  • Background: Staphylococcus aureus (s. aureus) and Escherichia coli (E. coli) are major pathogens in community and hospital. And they sometimes cause the outbreak in hospital in the immunocompromised patients. Pulsed-field gel electrophoresis (PFGE) has been regarded as a standard method for genotyping in epidemiologic studies, but it is laborious and time-consuming. Infrequent restriction site-polymerase chain reaction (IRS-PCR), a new genotyping methods, was performed to compare the applicability with PFGE. Methods: We performed PFGE and IRS-PCR on S. aurues (n=120) and E. coli (n=117) which were collected clinically in 4 different hospitals. We assessed each method in terms of discriminatory power, quality, and efficiency. Results: In E. coli, the discriminatory power of IRS-PCR was $46.7{\sim}86.7%$, and that of PFGE was $88.9{\sim}96.7%$ according to hospital. But in S. aurues, the discriminatory power of IRS-PCR was $20{\sim}56.7%$, and that of PFGE was $40{\sim}90%$ according to hospital. The typablity and reproducibility of IRS-PCR were 100% of each. PFGE needed four days to complete the procedure, but IRS-PCR could be performed within one day, IRS-PCR showed better resolution than PFGE. Conclusion: In case of gram negative bacteria (like E. coli), IRS-PCR could be a reliable alternative for epidemiologic typing due to better efficiency and comparable discriminatory power. But in the case of gram positive bacteria (like S. aureus), IRS-PCR does not seem to be suitable for the strain-to-strain differentiation. More trials and changes of restriction enzymes or primers could reveal the efficacy of IRS-PCR in the field of molecular typing.

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Transforming an Entity-Relationship Model into a Temporal Object Oriented Model Based on Object Versioning (객체 버전화를 중심으로 시간지원 개체-관계 모델의 시간지원 객체 지향 모델로 변환)

  • 이홍로
    • Journal of Internet Computing and Services
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    • v.2 no.2
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    • pp.71-93
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    • 2001
  • Commonly to design a database system. a conceptual database has to be designed and then it is transformed into a logical database schema prior to building a target database system. This paper proposes a method which transforms a Temporal Entity-Relationship Model(TERM) into a Temporal Object-Oriented Model(TOOM) to build an efficient database schema. I formalize the time concept in view of object versioning and specify the constraints required during transformation procedure. The proposed transformation method contributes to getting the logical temporal data from the conceptual temporal events Without any loss of semantics, Compared to other approaches of supporting various properties, this approach is more general and efficient because it is the semantically seamless transformation method by using the orthogonality of types of objects, semantics of relationships and constraints over roles.

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Electrochemical Performances of Acid-Treated and Pyrolyzed Cokes According to Acid Treatment Time (산처리 시간별 산화 코크스와 열분해 코크스의 전기화학적 거동)

  • Kim, Ick-Jun;Yang, Sunhye;Jeon, Min-Je;Moon, Seong-In;Kim, Hyun-Soo
    • Applied Chemistry for Engineering
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    • v.19 no.4
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    • pp.407-412
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    • 2008
  • As an activation procedure, in this study, the oxidation treatment of needle cokes with a dilute nitric acid and sodium chlorate $(NaClO_3)$, combined with heat treatment, was attempted. The structures of acid-treated and pyrolyzed coke were examined with XRD, FESEM, elemental analyzer, BET, and Raman spectroscopy. The behavior of double layer capacitance was investigated with the analysis of charge and discharge. The structure of needle coke treated with acid was revealed to a single phase of (001) diffraction peak after 24 h. On the other hand, thecoke oxidized by heat treatment was reduced to a graphite structure of (002) at $300^{\circ}C$. The distorted graphene layer structure, derived from the process of oxidation and reduction of the inter-layer, makes the pores by the electric field activation at the first charge, and generates the double layer capacitance from the second charge. The cell using pyrolyzed coke with 24 h acid treatment and $300^{\circ}C$ heat treatment exhibited the maximum capacitance per weight and volume of 33 F/g and 30 F/mL at the two-electrode system in the potential range of 0~2.5 V.

Fire Resistance Performance of High Strength Concrete with 4 Deformation Factors (4변형 인자에 의한 고강도콘크리트의 내화성능 평가)

  • Lee, Tae Gyu
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.16 no.5
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    • pp.112-120
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    • 2012
  • A numerical model considering the internal vaporization and the creep effect, in the form of a analytical program, for tracing the behavior of high strength concrete(HSC) members exposed to fire is presented. The two stages, i.e., spalling procedure and fire resistance time, associated with the thermal, moisture flow, creep and structural analysis, for the prediction of fire resistance behavior are explained. The use of the analytical program for tracing the response of HSC member from the initial pre-loading stage to collapse, due to fire, is demonstrated. Moisture evaporates, when concrete is exposed to fire, not only at concrete surface but also at inside the concrete to adjust the equilibrium and transfer properties of moisture. Finite element method is employed to facilitate the moisture diffusion analysis for any position of member, so that the prediction method of the moisture distribution inside the concrete members at fire is developed. The validity of the numerical model used in this program is established by comparing the predictions from this program with results from others fire resistance tests. The analytical program can be used to predict the fire resistance of HSC members for any value of the significant parameters, such as load, sectional dimensions, member length, and concrete strength.

Establishing Risk Management Process for Improved Business Value of a Multi-Purpose Building Project (복합 시설 프로젝트의 사업 가치 향상을 위한 리스크 관리 프로세스 구축 방안)

  • Lee, Jong-Sik;Cho, Seung-Ho
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.22 no.1
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    • pp.64-71
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    • 2018
  • Project Management Institute of America separates the types of risk with external risks and internal risks. The external risk is an uncontrollable risk in projects such as changes of policy and related systems, climate, natural disasters, exchange rates and so on. The internal risk is an existing risk in the project itself that is controllable items in the project. Technical risks in project management are cost, quality, time, safety and environment. Therefore, both the external and internal risks should be managed to perform the construction project successfully. In particular, we can secure the quality and safety of facilities through the technical risk management. The importance of potential risk management has been emerging as a major interest and the lack of risk management delays projects and increases construction costs with negative effects of the building safety since the complex building, which is composed of a great number of facilities, consists of many project units and there are conflicts between various participants and stake-holders. This study presents the ways of establishing risk management processes to ensure the safety of the complex building. To that end, establishing procedure of risk management processes is presented and types of risk and factors in construction projects and counter strategies are presented as available risk information on the stages.

Arthroscopic Treatment of Symptomatic Shoulders with Minimally Displaced Greater Thberosity Fracture (상완골 대결절의 미세전위골절의 관절경적치료)

  • Kim Seung-Ho;Ha Kwon-Ick
    • Clinics in Shoulder and Elbow
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    • v.2 no.2
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    • pp.178-186
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    • 1999
  • Twenty-three patients with chronic shoulder pain beyond 6 months after the fracture of the greater tuberosity underwent arthroscopic treatment and were retrospectively assessed after an average of 29 months(range, 22 to 40 months). There were 18 men and 5 women with the average age of 39 years(range, 24 to 61 years). Fourteen were isolated fractures and nine were related to acute anterior instability episode. The average displacement of the fracture was 2.3mm(range, 0 to 4mm) on the anteroposterior view of the plane radiographs. At the time of arthroscopy, all patients had partial thickness rotator cuff tears in the articular surface. The cuff tears were located on the tuberosity fracture area and were an Ellman's grade I to n in depth. With the arthroscopic debridement or repair of the tear depending on the condition of the tear itself, as well as the subacromial decompression, the UCLA score revealed good to excellent results in 20 and fair in 3 patients. Nineteen of the patients had returned to the previous level of activities. The patient with a higher activity demand revealed a lower level of activity return(p=0.034). The partial thickness rotator cuff tear should be considered in patients with chronic shoulder pain after the minimally displaced fracture of the greater tuberosity, and arthroscopic debridement or repair is an appropriate procedure.

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Studies on the Rapid Plasma Reagin(RPR) Card Test for the Diagnosis of Syphilis (매독진단(梅毒診斷)을 위(爲)한 신속(迅速)한 혈장항체검사법(血漿抗體檢査法)에 관(關)한 연구(硏究))

  • Kim, Joo-Deuk;Lew, Joon;Kim, Hyun-Joo
    • The Journal of the Korean Society for Microbiology
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    • v.3 no.1
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    • pp.15-23
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    • 1968
  • For the effective control of Syphilis, many investigators have developed a more rapid, simple and economical screening serological test which is adequately sensitive and specific. To fulfill the requirements of a more rapid serologic test for syphlis, a substitute for the conventional serum specimen was needed since considerable time and labor are involved in the processing of blood to serum. Burdon suggested the use of plasma in the serologic tests for syphilis as a substitute for serum. He noticed that plasma was more sensitive than serum in the Kline and Kahn tests, and attributed this to the presence of more antibody-like substance, "reagin" in plasma than in serum. However, to make plasma sufficiently sensitive, it was necessary to inactivate plasma by heating at a temperature of $56^{\circ}C$ for about 30 minutes. Heating of plasma resulted in the precipitation of fibrinogen which made centrifugation necessary to obtain dear plasma. Since the chief disadvantage to the use of unheated plasma(or serum) was a reduction in sensitivity of results-which probably was due to a labile factor such as complement-Portnoy et al began to consider rapid chemical methods of inactivation of plasma(or serum). They experienced that choline chloirde was shown to be anticomplementary which suggested its use as an inactivating agent for unheated plasma(or serum). In 1959 Portnoy et al reported the Rapid Plasma Reagin(RPR) Test for syphilis which is a more rapid, economical and simple. But still this test has many disadvantages as a rapid performing, field and office procedure, because it requires the usual laboratory equipments such as centrifuge, rotating machine, microscope etc. To substitute these disadvantages of the RPR test, in 1962, Portnoy et al developed the Rapid Plasma Reagin(RPR) card test for syphilis, which has the following advantages: a) Simplicity and rapidity of performance, b) Requires no laboratory equipments, c) Stable antigen suspension, d) Adequate sensitivity and specificity. This RPR card test can be used as a rapidly performing and screening test in field investigation, outpatient clinics, small laboratories and hospitals doing limited syphilis serology, and predonor in blood bank. Private clinic which has limited laboratory equipment and technic for syphilis serology can also use this RPR card test as a tool in the rapid diagnosis of syphilis. It was thought that this RPR card test is a useful tool in Korea for private physician and mass survey for syphilis diagnosis. But Portnoy patented the reagents needed for the performing the RPR card test. Therefore authors developed newly the reagents and according to Portnoy's method evaluated the newly developed. RPR card test compared with the VDRL, Kolmer CF, and RPCF tests. The RPR card and VDRL tests were performed plasma and serum from the total 1,132 cases. Among these 1,131 cases, 521 were syphilis suspected laboratory specimens, and 611 were syphilis unsuspected healthy young men. After screening with these two tests, the RPR card and VDRL tests, reactive specimens to the above one or both tests were retested by the Kolmer CF and RPCF tests.

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Effects of Gamma Irradiation and Methyl Bromide Fumigation on Quarantine Pest and Physical Quality of Apples (감마선과 methyl bromide 처리가 사과의 검역해충 및 물리적 품질에 미치는 영향)

  • Kang, Ho-Jin;Kwon, Yong-Jung;Byun, Myung-Woo;Kim, Hyun-Ku;Chung, Hun-Sik;Choi, Jong-Uook;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.783-790
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    • 2003
  • Tetranychus urticae Koch and Panonychus ulmi Koch were identified as quarantine pests in apple samples and were controlled soon after the commercial treatment of methyl bromide (MeBr) and with irradiation doses $(0.5{\sim}3kGy)$ after irradiation. The physical qualities of apples, such as rotting, withering, weight changes, hardness, and color, were apparently affected by MeBr. Furthermore, more than 2 kGy irradiation resulted from the lapse of storage time. Associated with the physical qualities of stored apples, irradiation and fumigation for quarantine purposes were more adequate after 40 days of storage at $0{\pm}1^{\circ}C\;(85{\pm}2%\;RH)$ immediately following harvest, rather than soon after harvest. Less than 1 kGy irradiation, based on its accumulated control effect on pests, can potentially be applied as a quarantine procedure without causing significant changes in the physical qualities of fresh apples, in contrast to MeBr.

Unaided Stapling Technique for Pure Single-Incision Distal Gastrectomy in Early Gastric Cancer: Unaided Delta-Shaped Anastomosis and Uncut Roux-en-Y Anastomosis

  • Suh, Yun-Suhk;Park, Ji-Ho;Kim, Tae Han;Huh, Yeon-Ju;Son, Young Gil;Yang, Jun-Young;Kong, Seong-Ho;Lee, Hyuk-Joon;Yang, Han-Kwang
    • Journal of Gastric Cancer
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    • v.15 no.2
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    • pp.105-112
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    • 2015
  • Purpose: Intracorporeal anastomosis is the most difficult procedure during pure single-incision distal gastrectomy (SIDG) that affects its generalization. We introduced unaided delta-shaped anastomosis (uDelta), a novel anastomosis technique, for gastroduodenostomy after pure SIDG, and compared the results with those of previously reported Roux-en-Y anastomosis (RY). Materials and Methods: Between March 2014 and March 2015, SIDG with D1+ lymph node dissection was performed for early gastric cancer through a 2.5-cm transumbilical incision without any additional port. uDelta was performed by the operator alone, without any intracorporeal assistance. Results: uDelta was performed on 11 patents, and uncut RY was performed on 5-patients without open or multiport conversion. R0 resection was performed in all cases. No significant differences were observed in mean age and body mass index between patients who underwent uDelta or RY. Mean operation times were $214.5{\pm}36.2$ minutes for uDelta and $240.8{\pm}65.9$ minutes for RY, which was not significantly different. Reconstruction time for uDelta was shorter than that for RY, with marginal statistical significance ($26.1{\pm}8.3$ minutes vs. $38.0{\pm}9.1$ minutes, P=0.05). There were no intraoperative transfusions, 30-day mortality, or anastomosis-related complications in either group. Average length of hospital stay was $8.2{\pm}1.9$ days in the uDelta group and $7.2{\pm}0.8$ days in the RY group (P=0.320). Conclusions: After carefully considering indications, uDelta can be a feasible and can be a reproducible reconstruction method after SIDG in early gastric cancer.

A STUDY OF THE ANTI-ROTATING INNER POST SCREW SYSTEM AS A MEANS OF PREVENTING ABUTMENT SCREW LOOSENING (회전 방지용 Post Screw 시스템의 임플랜트 지대나사풀림 방지효과에 관한 연구)

  • Kim Jong-Hui;Lim Ju-Hwan;Cho In-Ho;Lee Joon-Seok
    • The Journal of Korean Academy of Prosthodontics
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    • v.43 no.5
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    • pp.671-683
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    • 2005
  • Statement of problem: The most commonly reported problem associated with dental implant restoration is the loosening of the screws. Purpose: This study compared the efficacy of an implant system incorporating an anti-rotational locking sleeve(Anti-Rotating Inner Post Screw System(ARIPS-system)) with other, traditional implant systems as a means of minimizing vibration loosening. Materials and methods: Three implant systems were examined; the conventional external hex type, the ARIPS-system, and the internal taper type implant system 30 specimens(10 samples per group)were fabricated and each abutment screw was secured to the implant future with 32Ncm of torque force and loosening torque was measured using a Torque Gauge. The procedure was repeated 3 times, recording initial loosening torque each time. The re-tightened abutment screw was subjected to a cyclic load having a maximum forte of 200N and minimum of 20N at 2Hz over a period of 12,600 cycles. after which the loosening torque was measured. Measured values were calaulated for statistical analysis. Analysis of measured value was performed by 3 methods: (i) as a percentage average of the initial 3 loosening-torque values(initial loosening value) to the tightening torque of 32Ncm, (ii) as a percentage of the loosening torque value after a load of 200N(experimental value) to the initial loosening value, and (iii) as a percentage of the experimental value to the 32Ncm of tightening torque. The analyses shows the amount of initial loosening at the screw, loosening by repetitive load and the the final loosening value. Results: The results of this study were as follows (1) Percentage of initial loosening value to tightening-torque was increased in order of external hex, ARIPS-system and internal taper and all values between each groups showed statistical significance (p<0.05). (2) Percentage of experimental value to initial loosening value was increased in order of external hex, ARIPS-system and internal taper. Value of internal taper showed significant difference with those of external hex and ARIPS-system (p<0.05). (3) Percentage of experimental value to tightening torque was increased in order of external hex, ARIPS-system and internal taper and all values between each groups showed statistical significance (p<0.05). Conclusion: The results of the analysis of the final loosening level value, which are closely correlated to clinical use, show that the ARIPS-system can be a useful means of minimizing abutment screw loosening when compared to the external hex type system. Although further clinical studies need to be made, the ARIPS-system should be considered to maximize the long-term success of the implant prosthesis.