• BMB Reports
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• v.35 no.2
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• pp.239-243
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• 2002

Angiotensin I that converts the enzyme (ACE) inhibitory peptide, Gly-Pro-Leu, previously purified and identified from the Alaskan pollack skin gelatin hydrolysate, were synthesized. In addition, the peptides Gly-Leu-Pro, Leu-Gly-Pro, Leu-Pro-Gly, Pro-Gly-Leu, Pro-Leu-Gly, Gly-Pro, and Pro-Leu, which consisted of glycine, proline, and leucine, were synthesized by the solid-phase method. The $IC_{50}$ values of each tripeptide - namely Leu-Gly-Pro, Gly-Leu-Pro, Gly-Pro-Leu, Pro-Leu-Gly, Leu-Pro-Gly, and Pro-Gly-Leu - were 0.72, 1.62, 2.65, 4.74, 5.73, and $13.93{\mu}M$, respectively. The ACE inhibitory activity of these tripeptides was higher than that of dipeptides, such as Gly-Pro and Pro-Leu with $IC_{50}$ values of 252.6 and $337.3\;{\mu}M$, respectively. Among the tripeptides, Leu-Gly-Pro and Gly-Leu-Pro had higher inhibitory activity than Gly-Pro-Leu that was isolated from the Alaskan pollack skin gelatin hydrolysate. Among the different types of tripeptides that were examined, the highest ACE inhibitory activity was observed for Leu-Gly-Pro. It had the leucine residue at the N-terminal and proline residue at the C-terminal.

• Journal of the Korean Home Economics Association
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• v.42 no.11
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• pp.151-165
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• 2004

The purpose of this study was to investigate the influence of adolescent's environmental education experience and peer group on pro-environmental consumption behavior The survey study administered 610 questionnaires to general high school students in Daegu. The main findings of this study were as follows. First, adolescent's pro-environmental purchase behavior was positively correlated to the degree of participation in environment-related events in school, the mother's pro-environmental purchase, use and disposal behavior and environment education experience in society, while the peer group's pro-environmental purchase was positively correlated to use and disposal behavior Also adolescent's pro-environmental use behavior was positively correlated to the mother's pro-environmental purchase, use and disposal behavior, the peer group's pro-environmental purchase, and use and disposal behavior. Adolescent's pro-environmental disposal behavior was positively correlated to the degree of participation in environment-related events in school, the mother's pro-environmental purchase, use and disposal behavior, environment education experience in society, the peer group's pro-environmental purchase, and use and disposal behavior. Second, adolescent's pro-environmental purchase behavior was influenced to the greatest extent by the peer group's pro-environmental purchase behavior, followed by the mother's pro-environmental purchase behavior and use behavior, and to a lesser extent, tv environment education experience in society. Also adolescent's pro-environmental use behavior was influenced to the greatest extent tv the mother's pro-environmental use behavior, followed by the peer group's one, and to a lesser extent, by the mother's pro-environmental disposal behavior. Adolescent's pro-environmental disposal behavior was influenced to the greatest extent by the mother's pro-environmental disposal behavior, followed tv the peer group's one, the mother's pro-environmental purchase behavior and, to a lesser extent, by the degree of participation in environment-related events in school.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.10
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• pp.4909-4914
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• 2012

Objective: The aim of this Human Genome Epidemiology (HuGE) review and meta-analysis was to derive a more precise estimation of the association between p53 codon 72 polymorphism (Arg72Pro, rs1042522 G>C) and cervical cancer risk among Asians. Methods: A literature search of Pubmed, Embase, Web of Science and CBM databases from inception through June 2012 was conducted. The meta-analysis was performed using STATA 12.0 software. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of any association. Twenty-eight case-control studies were included with a total of 3,580 cervical cancer cases and 3,827 healthy controls. When all the eligible studies were pooled into the meta-analysis, the results showed that the Pro/Pro genotype was associated with increased risk of cervical cancer under the heterozygous model (Pro/Pro vs. Arg/Pro: OR = 1.25, 95%CI: 1.02-1.53, P= 0.005). However, no statistically significant associations were found under four other genetic models (Pro vs. Arg: OR = 0.97, 95%CI: 0.85-1.10, P= 0.624; Pro/Pro + Arg/Pro vs. Arg/Arg: OR = 0.84, 95%CI: 0.70-1.01, P= 0.058; Pro/Pro vs. Arg/Arg + Arg/Pro: OR = 1.13, 95%CI: 0.92-1.39, P= 0.242; Pro/Pro vs. Arg/Arg: OR = 0.97, 95%CI: 0.76-1.22, P= 0.765; respectively). In the subgroup analysis based on country, the Pro/Pro genotype and Pro carrier showed significant associations with increased risk of cervical cancer among Indian populations, but not among Chinese, Japanese and Korean populations. Conclusion: Results from the current meta-analysis suggests that p53 codon 72 polymorphism might be associated with increased risk of cervical cancer, especially among Indians.

• Journal of Life Science
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• v.27 no.6
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• pp.632-639
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• 2017

Mature haptoglobin (Hp) is a plasma glycoprotein and acts as an antioxidant by scavenging cell-free hemoglobin (Hb). Prohaptoglobin (proHp) is an unprocessed Hp precursor which is present a little in circulation. However, the biological function of proHp remains unknown. To investigate the structural and functional differences between proHp and Hp, we prepared recombinant proHp isoforms and compared their sialic acid content and Hb-binding capacity with those of mature isoforms. When proHp samples were analyzed by Western blot under non-reducing conditions, proHp1 was detected as one band of approximately 130 kDa and proHp2 as multiple bands >200 kDa, in the manner of mature Hp1-1 and Hp2-2, respectively. On the native polyacrylamide gel under non-reducing and non-denaturing conditions, both proHp isoforms migrated more slowly than their mature Hp counterparts. In addition, the lectin-based ELISA assay demonstrated that the content of sialic acid in proHp1 and proHp2 was much less than in Hp1-1 and Hp2-2. The Hb-binding capacity of proHp was also lower than those of mature Hp. These findings indicate that proHp and Hp are similar in the size and polymerization pattern, but different in sialic acid content and Hb-binding activity. It suggests precursor proHp may exert different functions in circulation than does mature Hp.

• Restorative Dentistry and Endodontics
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• v.30 no.4
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• pp.294-302
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• 2005

The purpose of this study was to evaluate the canal configuration after shaping by ProFile. ProTaper and K-Flexofile in simulated resin canals with different angles of curvature. Three types of instruments were used: ProFile. ProTaper. K-Flexofile. Simulated root canals. which were made of epoxy resin. were prepared by ProFile. ProTaper with rotary instrument using a crown-down pressureless technique. and hand instrumentation was performed by K-Flexofile using a step-back technique. All simulated. canals were prepared up to size 25 file at end-point of preparation. Pre and post instrumentation images were recorded with Scanner. Assessment of canal shape was completed with Image Analysis program. Measurements were made at 1. 2. 3. 4. 5. 6. 7. 8. 9 and 10mm from the apex. At each level. outer canal width. inner canal width. total canal width. and amount of transportation from original axis were recorded. Instrument deformation and fracture were recorded. Data were analyzed by means of one-way ANOVA analysis of variance and the Sheffe's test. The result was that ProFile and ProTaper maintain original canal shape regardless of the increase of angle of curvature than K-Flexofile. ProFile show significantly less canal transportation and maintained original canal shape better than ProTaper.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.3
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• pp.172-180
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• 2020

This study investigated retrospectively the correlation between the results of the N-terminal pro-brain natriuretic peptide (NT-proBNP) and a routine blood test using a hospital information system. The NT-proBNP is involved in the pathophysiology of heart failure. The results show that the relationship between age and NT-proBNP was significant (P<0.01) with a positive correlation (r=0.163). The peptide concentration showed a negative correlation between the total protein (r=-0.250) and albumin (r=-0.270), and a negative correlation between the erythrocyte count and hemoglobin and hematocrit (P<0.01). NT-proBNP had a positive correlation with neutrophils (r=0.227) and a negative correlation with lymphocytes (r=-0.236), showing significant results (P<0.01). NT-proBNP and creatinine showed a positive correlation (r=0.594, P<0.01), and it was the most influential factor according to multiple regression analysis (B=0.53, t=7.65). P<0.01). The concentrations of NT-proBNP and uric acid showed a positive correlation (r=0.180, P<0.05). Lactate dehydrogenase was observed as a factor affecting the NT-proBNP (B=0.20, t=3.28, P<0.01). This explanatory power had an influence of 43%. Therefore, the accurate test and related factors of the NT-proBNP have significant clinical value.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.17 no.1
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• pp.100-113
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• 2018

The Proximity Service, which is one of the most popular network capacity improvement methods, uses the frequency reuse in order to increase the frequency efficiency. As a result, inter-cell interference between cellular and proximity service users occurs at a cell edge. In this paper, we proposed a mitigation scheme for inter-cell interference, where we suggested a new function of and eNB with ProSe function exchanging information about ProSe parameters and ProSe user equipment with neighboring cells via the X2 interface. As the first step, the resource which did not cause the inter-cell interference problem were pre-allocated through the frequency sensing in the ProSe direct discovery. As the next step, the inter-cell interference problem was solved by reallocating appropriate resources based on the ProSe application code, the ProSe application QoS, the ProSe application ID and validity timer in ProSe direct communication.

• Korean Journal of Microbiology
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• v.45 no.3
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• pp.263-267
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• 2009

An RpoS factor is a transcriptional regulator which participates in numerous biological processes. In this work, we investigated the transcriptional regulation of proBA and proC composing proline biosynthetic pathway in Escherichia coli. While the proBA and proC genes were greatly induced in an exponential growth phase, they were dramatically repressed in a stationary growth phase in the wild type E. coli. Unlike the wild type E. coli, the proBA and proC genes were not repressed even in the stationary growth phase in its isogenic rpoS mutant. These results suggest that the RpoS factor acts as a transcriptional repressor of proBA and proC genes. The production of threonine, methionine, lysine, and arginine in the rpoS mutant were also increased by more than two times compared to its parental wild type, suggesting that the mutant is able to be used as an useful host strain for the amino acid overproduction.

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.99.2-99.2
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• 2002

• Journal of the Korean Home Economics Association
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• v.41 no.12
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• pp.169-186
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• 2003

The purpose of this study were (1) to examine the level of housewives' pro- environmental behavior, and (2) to analyze the effect of the selected variables on pro-environmental behavior 941 sample were selected from housewives living in urban area. For the analysis of data, frequencies, means, one-way ANOVA, DMR test, Chi-square test, and multiple regression analysis were used. The major findings were as follows：1. The level of housewives' pro-environmental behavior was lower than that of pro-environmental attitude. 2. The level of housewives' pro-environmental behavior was mainly affected by their environment-related variables, for example, pro-environmental attitude, and experience of environmental education. Most of socio-demographic variables and family-related variables didn't have significant effects on the level of pro-environmental behavior.