• Journal of Bio-Environment Control
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• v.22 no.2
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• pp.188-191
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• 2013

This study was carried out to investigate the effects of light, temperature, and priming treatment on the germination of Bidens bipinnata seeds for the purpose of cultivation of new vegetable crop. The results showed that the treatment of $25^{\circ}C$ yielded the highest germination percentages and the highest germination speed in both light and dark conditions. Light condition had no effect on the germination of B. bipinnata. Priming treatments demon-strated the following improved germination percentages: $Ca(NO_3)_2$ 0.1 M (68.3%), $K_3PO_4$ 0.1 M (63.3%), and $KNO_3$ 0.1 M (53.3%), with $Ca(NO_3)_2$ 0.1 M treatment giving the greatest improvement. Consequentially, the results suggested that $25^{\circ}C$ light condition, and $K_3PO_4$ 0.1 M or $Ca(NO_3)_2$ 0.1 M priming treatments would be effective method to improve the germination of Bidens bipinnata seeds.

• Korean Journal of Medicinal Crop Science
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• v.9 no.2
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• pp.99-107
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• 2001

Germination of Codonopsis pilosula seeds and afterward their seedling emergence have been occasionally very poor due to their dormancy and structural defects. The study was done to determine the treatment effects of priming, $GA_3$, agitation and washing during and after priming, drying and water imbibition after drying on germination of its seeds and then of the successive three combined treatment after priming on the its seedling emergence to model its presown seed treatment. While agitation at the speed of 10 rpm was made or not, priming using $Ca(NO_3)_2$ and $GA_3$ under their different concentrations and light quality illuminated for 12 hours a day were done separately. Then their two best results were compared to determine the better one. Using the above determined best result, washing was done 3 minutes in the tap water or not, and drying of washed seeds was made under 4 different lights, and water imbibition of the dried seeds with differed period were successively done to deduce its presown seed treatment. In the comparison of the best germination rate of priming and $GA_3$, the former showed considerably higher rate than the latter; the priming treatment was done at agitated $Ca(NO_3)_2$ 150 mM solution under darkness or 12 hour red light illumination a day for 2 days. Washing after priming enhanced the rate compared to no-washing. Drying was the best way at which seeds washed were desiccated under $35^{\circ}C$ and 4 hour red light illumination. Water imbibition immediately before sowing must be made for 2 days. Seedling emergence rate was the highest at the seeds from successive 4 treatments, priming, washing, drying and water imbibition before sowing of two others, the priming and washing, or the priming, washing and water imbibition, meaning that its presown seed treatment must follow the procedure of the above 4 successive ones.

• Korean Journal of Medicinal Crop Science
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• v.5 no.2
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• pp.139-146
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• 1997

Priming has been used to establish a better standing in practice as controling the seed moisture content after sowing. The experiment was done to measure the effect of priming (material ; concentration ; period) and light quality (red ; white ; dark) after sowing on seed germination and radicle elongation of Campanulaceae (Platycodon grandiflorum ; Codonopsis lanceolata ; C. pilosula) to give an information on their earlier standing establishment. The germination test was carried out with 12 hours irradiation for 9 days after priming treatment. In the darkness, the mean germination rate of all the species was decreased in the order to P. grandiflorum, C. pilosula, C. lanceolata. Their germination and radicle elongation became more inclined when primed with $Ca(CO_3)_2$ or with 50 to 150mM than with $KNO_3$ or no priming although there was no difference between priming periods. Under irradiation during their seed germination, however, the former order was changed to P. grandiflorum, C. lanceolata, C. pilosula because light quality treatment given after priming reduced the rate of C. pilosula but enhanced that of C. lanceolata. Although light quality forced after priming did not affect the mean germination rate of P. grandiflorum, it increased earlier or alltime germination of C. lanceolata or C. pilosula, respectively. White light after priming repressed germinations of C. lanceolata and C. pilosulai but increased earlier germinatin of P. grandiflorum, meaning that seed germination and radicle elongation of Campanulaceae could be determined by light quality treated after priming.

• Journal of Life Science
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• v.13 no.4
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• pp.433-440
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• 2003

Osmotic and solid matrix priming treatments enhanced germination performance. We compared osmotic with solid matrix priming to determine the more effective treatment for improving seed germination in pepper and tomato. Seed hydration was immediately observed after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in the two vegetable crops. Osmotic priming and solid matrix priming did not increased percent germination, but showed shorter number of days to 50% of the final germination percentage ($T_{50}$) compared with untreated seeds, regardless of germination temperature. $T_{50}$ value was reduced in osmotic or solid matrix primed pepper seeds about 6.0, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and $30^{\circ}C$, respectively. While, that in tomato seeds was reduced about 3.3, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and $30^{\circ}C$, respectively. The effectiveness of osmotic priming or solid matrix priming in reducing the $T_{50}$ was greater when the seeds were germinated at $15^{\circ}C$ than at temperature of higher than $20^{\circ}C$. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in pepper. However, there was no significant difference on the percentage germination between solid matrix and osmotic primed seeds in tomato. After priming, dried-bark seeds showed faster germination than surface-dried seeds in pepper. On the other hand, there was no significant difference in tomato. Emergence of pepper and tomato seeds was markedly enhanced by osmotic priming or SMP treatment although the final emergence percentage was not significantly influenced. On the other hand, early growth was not significantly influenced by osmotic priming or SMP treatment of pepper and tomato seeds.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.307-313
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• 1997

Seed germination test done in laboratory does not coincide with field emergence in general. The experiments were carried out to examine the effect of priming and $GA_3$, treatment to seeds of Platycodon grandiflorum; Codonopsis lanceolata and C. pilosula on lapsed time to first seedling emergence, seedling emergence, morphological characters and growth and the cause of poor emergence of C. pilosula. No-treatment as Control (water), priming or $GA_3$ treatment was done with only distilled water for 2 days, $CA(NO_3)_2$ 150 mM for 2 days or $GA_3$ 0.1 mM for 3 days, respectively. Seedling emergence rate was counted every 2 days but morphological characters and dry weight of shoot and root were measured on 38 days after sowing. Their internal seed structures were examined with Scanning Electron Microscope. C. pilosula had poorer seedling emergence rate than P grandiflorum and C. lanceolata showing nearly same rate: Compared to the other treatment (s) P. grandiflorum displayed higher rate in priming and $GA_3$, treatments but C. lanceolata or C. pilosula did the greatest rate in only $GA_3$ or priming treatment, respectively. $GA_3$ treatment to seeds of P. grandiflorum and C. lanceolata shortened the lapsed time to seedling emergence in comparison with Control, 2-days water imbibition before sowing. In all the species plant height and number of leaves per seedling became shorter and less in priming treatment than the other treatments except plant height of C. Pilosula while their hypocotyl length was nearly same in all treatments. Although priming treatment had nearly similar effect to morphological characters, $GA_3$ treatment forced greater shoot, root and aftermath total dry weight per seedling. Poor seedling emergence of C. pilosula was caused by its seed defect like cleavage or lack of embryo, poor development of embryo and endosperm or their separation.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.213-213
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• 2017

High quality seed of proso millet that has high germination percentage, germination speed, and uniformity demanded to increases rates of mechanization in cereal crop cultivation. In order to improve germination characteristics, proso millet seeds were treated with red light, ultrasonication and liquid smoke (LS) solution that generated from hickory wood. All treatments were performed in seed priming solution with 100mM $NH_4NO_3$ at $15^{\circ}C$ for 24hrs under aeration condition. Seeds were exposed under light intensity of 2000 lux for 15m, 30m, 60m, and 120m in priming solution. Ultrasonic treatment was performed at 60%, 80%, and 100% intensity of 21.6 KHz for 5m, 10m, and 20m in priming solution. For LS treatment seed were soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted solution with $dH_2O$ or 100mM $NH_4NO_3$ solution. The effect of seed treatment was evaluated with germination percentage (GP), mean germination time (MGT), germination index (GI), germination rate (GR), Germination uniformity (GU) and heath seed percentage (HS). Our results demonstrate that red light (15min) or ultrasonication (21.6kHz, 5min) treatment improved MGT, GI, GR, and GU comparing to untreated control. Importantly, we show that LS treatments have significant effect on the health seedling and germination characteristics. Proso millet seeds that treated with 5% LS solution for 24hrs improves HS up to 97% that similar results obtained in 100mM $NH_4NO_3$ priming for 24hrs. The combined treatment with LS solution and 100mM $NH_4NO_3$ priming were not effective in all treatments. Our results demonstrate that treating seeds with LS or 100mM $NH_4NO_3$ priming or ultrasonication improves germination characteristics. The methods described here will help advance research using this species by increasing the germination performance at which successive seed pellet process.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.68-75
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• 2001

Reasonable seedling emergence is a prerequisite for successful crop cultivation especially in medicinal crops. The study was carried out to model the pretreatment or seed treatment of Codonopsis lanceolata seeds by evaluating the treatment effects of priming, $GA_3$, drying and water imbibition after drying on their germination and then their successive seed treatment on the basis of its seedling emergence. The priming using $Ca(NO_3)_2$ and $GA_3$ treatment under their different concentrations and light quality illuminated for 12 hours a day were separately done and the two best results from priming and $GA_3$ treatments were compared to determine the better one. The drying of imbibed seeds using the above best result and water imbibition of the dried seeds were successively done to measure the rates of germination and emergence. On the greatest germination rate of the first two individual treatments, priming was obtained at $Ca(NO_3)_2$ 150 mM under blue, red light or darkness for 2 days but $GA_3$ was done at 0.1 mM under blue light imposed for 3 days. However, the result of the latter $GA_3$ treatment was better than that of priming. $GA_3$ treated seeds were best desiccated under $35^{\circ}C$ and 4 hour red light illumination. A day water imbibition immediately before sowing increased the germination rate of seeds dried after $GA_3$ treatment. Seedling emergence tested after all 3 successive treatments, $GA_3$, drying and water imbibition before sowing was greater than the two others, only $GA_3$ treatment and the combination of $GA_3$ and drying, meaning that its pretreatment of seeds or seed treatment must follow the successive procedure of the above 3 ones.

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.04a
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• pp.123-124
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• 1998

• Weed & Turfgrass Science
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• v.3 no.3
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• pp.232-239
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• 2014

Zoysiagrass seeds had low germination or lack of germination uniformity under natural condition. To improve the final germination percentage of zoysiagrass seeds, we investigated the methods of proper pre-treatment to enhance germination rate of zoysiagrass. Seeds were subjected to the testa scarification and four types of seed priming methods, namely, hydro priming (distilled water), osmotic priming (polyethylene glycol -0.5 MPa, -1.0MPa, -2.0 MPa), halo priming (Potassium nitrate 100 mM, 200 mM, 400 mM), and solid matrix priming (seed : microcel-E : distilled water = 4:1:8) with and without 25% KOH treatment in the testa. The scarification improved the germination rate and reduced the germination time. Osmotic priming (PEG -0.5 MPa) with scarification were significantly increased the seed germination rate and decreased MGT, and $T_{50}$. Seed germination rate obtained by osmotic priming (PEG -0.5MPa) with scarification increased above 60% in comparison with natural condition. Conclusively, scarified and primed zoysiagrass seed germinated more rapidly and uniformly in the study. The present results suggested that PEG priming treatment with KOH treatment prior to sowing could improve germination percentage, and this method makes us establish the early zoysiagrasses in the field.

• Horticultural Science & Technology
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• v.18 no.3
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• pp.327-333
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• 2000

This experiment was conducted to determine the effect of priming duration and temperature on germinability of vegetable seeds. Priming chemicals were -0.50 MPa PEG 8000 for carrot, 50 mM $K_3PO_4$ for lettuce, 200 mM $KH_2PO_4$ for onion, and 100 mM $Ca(NO_3)_2$ for Welsh onion seeds. Priming temperature varied from $10^{\circ}C$ to $25^{\circ}C$, and priming duration varied from 12 hours to 5 days depending on crops. Percent germination of carrot seeds was significantly affected by the seed treatment and priming temperature. Priming at $20^{\circ}C$ for 3 days was most effective in reducing number of days to attain 50% of the final germination percentage (T50) and mean number of days to germination (MDG). Radicle protrusion in the priming solution was as high as 14% when primed at $20^{\circ}C$ for 4 days. Percent germination of lettuce seeds was affected significantly by the seed treatment and priming duration. Priming lettuce seeds at $20^{\circ}C$ for 2 days increased percent germination and shortened T50 and MDG without radicle protrusion. Percent germination of onion seeds was affected significantly by the seed treatment, priming duration, and temperature. Priming at either $10^{\circ}C$ or $15^{\circ}C$ for 3, 4, or 5 days did not affect percent germination of onion seeds, but was effective in reducing T50 and MDG. Decline in percent germination of onion seeds was precipitous as the length of priming duration increased from 3 to 5 days. Percent germination of welsh onion seeds was not affected by the priming duration and temperature. Priming at $15^{\circ}C$ for 4 days was most effective in reducing T50 and MDG, increased in duration of priming at $15^{\circ}C$ or $20^{\circ}C$ increased radicle protrusion.