• Title/Summary/Keyword: Priming time

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Leakage of Seed Reserve Nutrient in Artificially Aged Pepper Seeds and Enhancement of Seed Vigor by Priming (노화처리된 고추종자의 저장성분 누출과 priming 처리가 종자활력 증진에 미치는 영향)

  • Kang Jum-Soon;Choi In-Soo
    • Journal of Life Science
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    • v.16 no.2 s.75
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    • pp.352-356
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    • 2006
  • Quantity of protein, amino acid, and sugar leaked from seeds was greater as the viability of seeds was dropped by the time elapsed of seed aging treatment. In the seeds with the artificial aging treatment for 20 days, 35.8 mg of protein was leaked on the 4th day after soaking, which was 6.9 times higher than that of control. Leakage of amino acid was also higher from low quality seeds treated with the aging treatment. In the seeds with the aging treatment for 20 days, $36.5{\mu}g$ fig of sugar was leaked on the 4th day after soaking, which was 2.8 times higher than that of control. The leakage of inorganic compound was higher from the low quality seeds, and leakage of total protein, amino acid, and sugar. According to .the quantity of leakage, water soluble compounds, which can be used for the assessment of seed quality without any destruction, were protein and potassium. Germination rate and percentage of seeds were dropped with the seed aging treatment, and the seed viability could be recovered by priming treatment. This phenomenon was very clear when the low quality seeds were germinated at low temperature.

The Lexical Access of Regular and Irregular Korean Verbs in the Mental Lexicon (한국어 규칙 동사와 불규칙 동사의 심성 어휘집 접근 과정)

  • Park, Hee-Jin;Koo, Min-Mo;Nam, Ki-Chun
    • Korean Journal of Cognitive Science
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    • v.23 no.1
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    • pp.1-23
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    • 2012
  • This study investigated the lexical access processing of inflected Korean verbs in the mental lexicon. In Korean, verbs can be classified into two main types of inflections, which are regular and irregular inflections, which can be further divided into three types of regular inflections and two types of irregular inflections. A masked priming lexical decision task was used and the priming effects were compared. Experiments were carried out using the five different types of verbal inflections in Korean: (1) No change-regularity (regular verbs with no orthographical or phonological changes), (2) Phonological change-regularity (regular verbs with phonological changes to the stem only), (3) Orthographical change-regularity (regular verbs that only undergo orthographical changes), (4) Stem change-irregularity (the stem is omitted or alternated with the other phoneme of the stem in irregular verbs), (5) Ending change-irregularity (irregular verbs with changes in the endings by phoneme substitution). The first three types are regarded as regular verbal inflections whereas the latter two types are regarded as irregular verbal inflections. The infinitive forms of the verb were presented as target words and three different conditions were presented as prime words. The three conditions included regular verbal inflection, irregular verbal inflection, and a control condition in which morphologically and semantically unrelated primes were presented. In addition, different stimulus onset asynchrony (SOA) were manipulated (43ms, 72ms, 230ms) to examine the time frame of the morphological decomposition process in word recognition. The results revealed that there were significant priming effects in all three SOAs across conditions. Hence, there was no significant differences in priming effects between regular and irregular verbal inflection conditions. This may suggest that Korean verb processing does not adopt different processing routes for regular and irregular inflections, which can also be an indication of earlier morphological information processing for Korean verbs.

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The effect of osmotic potentials on the temperature response for germination of pepper seed

  • Kim, Young Ae;Park, Sunyeob;Kim, Du Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.216-216
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    • 2017
  • Primed seeds germinate faster and uniformly, since these have already started imbibition and metabolism requiring to complete germination. Several factors such as solution composition, osmotic potential, and treatment duration affect seed priming response. Water potentials of priming solution and germination response of primed seed were investigated to clarify the effects different inorganic salt types and the duration. Pepper seeds were primed in osmotic solutions that were osmotic potential ranged -1.54 to -0.45 MPa in an aerated solution of polyethylene glycol 8000 (PEG; 17%, 22%, 27%), and inorganic salt solution of $KNO_3$, $K_2SO_4$, and $Na_2SO_4$ (100, 200, 300mM) in aerated condition. The seeds were treated at $20^{\circ}C$ for 4, 6 day (D) and stepwise duration combined concentration of 100mM (2 or 4 days) and 300mM(2 or 4days). After soaking treatment, seeds were washed with distilled water and then were dried to approximately 6% moisture content in dry chamber at $25^{\circ}C$. The germination of seed was characterized by ISAT rules at $20^{\circ}C/30^{\circ}C$ and $15^{\circ}C$. Total germination percentage (GP), mean germination time (MGT), germination uniformity (GU), germination rate (GR), and health seedling percent (HS) were calculated on the germinated seed in a 14-day period. Seed water potential (${\psi}$) was correlated with water potential of priming solution ($r^2=0.84$). The effect of seed priming on germination varies with inorganic salt and organic agents (PEG). Germination percentage (GP, 77 and 73%), GR (21.3 and $19.4 %{\cdot}day^{-1}$), and HS (58 and 52 %) was greatest and lowest MGT (4.2 and 4.8 day) when they were primed in 100mM $K_2SO_4$ (${\psi}=-0.45MPa$), and 100mM $KNO_3$ (${\psi}=-0.52MPa$) for 6 days compared to untreated control (67% GP, $19.7%{\cdot}day^{-1}\;GR$, 18% HS, and 10.8 day MGT) in $15^{\circ}C$ chamber. Water potential less then -1.2MPa of osmotic solution resulted lower GP, GR and HS and in $K_2SO_4$ and $KNO_3$. However, stepwise treatment of water potential that changed solution as 100mM for 4 D + 300mM for 2 D or 300mM for 4 D + 100mM for 2 D) were not better than single concentration alone in germination characteristics.

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THE EVALUATION OF MICROLEAKAGE OF PIT AND FISSURE SEALANT BY USAGE OF SELF-ETCHING ADHESIVE SYSTEM (자가 산부식 접착제를 이용한 치면열구전색의 미세누출 평가)

  • Kim, Hyun-Jin;Lee, Nan-Young;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.2
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    • pp.216-224
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    • 2008
  • The purpose of this study was to evaluate the effectiveness of fissurotomy and double application time of acidic primer of self-etching adhesive system of acid treatment on enamel surfaces for prevention of microleakage of pit and fissure sealants. The microleakage of pit and fissure sealants was evaluated by measuring the penetration depth of methylene blue solution. Specimens were divided by 4 groups according to the method of treatment. Group I: 35% phosphoric acid etching, 20 seconds. Group II: Priming with self-etching primer, 20 seconds. Group III: Priming with self-etching primer, 40 seconds. Group IV: Priming with self-etching primer, 20 seconds, after fissurotomy. The etched pattern produced on enamel was observed using a scanning electron microscope. Obtained data were analysed statistically using Kruscal-Wallis test followed by Mann-Whitney test for comparison of groups. The results are as follows: 1. Microleakage scores of group IV priming with self-etching primer 20 seconds after fissurotomy was the lowest. 2. Microleakage scores between group II and group III were not shown significant difference. 3. Enamel for 20 seconds etching with 35% phosphoric acid was observed the most effective etching pattern. And the etching pattern on the fissure enamel with self-etching adhesive 20 seconds after fissurotomy was more prominent than group II, III only using self-etching primer.

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Adaptive Response Induced by Low Dose Ionizing Radiation in Human Lymphocytes (인체 말초 혈액 림프구에서 저선량 방사선 조사에 의해 유도되는 적응 반응)

  • Kim, Jeong-Hee;Lee, Kyung-Jong;Cho, Chul-Koo;Yoo, Seong-Yul;Kim, Tae-Hwan;Kim, Sung-Ho
    • Journal of Radiation Protection and Research
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    • v.20 no.2
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    • pp.97-102
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    • 1995
  • Adaptive response induced by low dose ${\gamma}-ray$ irradiation in human peripheral lymphocytes was examimed. Human lymphocytes were exposured to low dose of ${\gamma}-ray$ (priming dose, 0.01Gy) followed by high dose (challenging dose, 1.5Gy) after various time intervals (4, 7, 20 hours). Frequencies of micronuclei were enumerated in both primed and unprimed groups. Maximum reduction in frequency of micronuclei was observed when challenging dose irradiation was followed by priming dose after 4hr incubation period. When challenging doses were irradiated 7 or 20hr after priming dose, frequencies of micronuclei were reduced slighty. However, these reduction were not statistically significant. In this study, human peripheral lymphocytes were irradiated at Go phase and they showed adaptive response induced by low dose radiation. Since micronucleus assay is relatively simpler and faster than other methods, it may be a good tool for evaluating radiation-induced adaptive responses.

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A Study on the Temperature-Dependent Discharge Characteristics in Alternating Current Plasma Display Panel (AC PDP의 온도에 따른 방전 특성 연구)

  • Lee, Seok-Hyun;Kim, Jee-Yong
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.56 no.3
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    • pp.577-582
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    • 2007
  • The plasma display panel is an image expression display using gas discharge plasma. However, gas discharge characteristics vary with temperature as gas discharge is sensitive to temperature. The discharge time lag extends a lot in low temperature and it is known as the cause which hinders high speed addressing which is essential for the size enlargement of the panel. Accordingly this research aims at identifying the temperature-dependent discharge characteristic. The lower temperature becomes, the longer addressing discharge time lag becomes. Particularly the statistical time lag extends much in low temperature. The increasing of electric field shortens discharge time lag in low temperature. Also, when priming particles are sufficiently supplied, stable discharge can be performed regardless of the influence of temperature.

Minimized Priming Volume for Open Heart Surgery in Neonates and Infants (신생아와 유아 심장 수술 시 심폐기회로 충진액의 최소화)

  • Kim, Woong-Han;Chang, Hyoung-Woo;Yang, Sung-Won;Cho, Jae-Hee;Lee, Kyung-Hoon;Baek, In-Hyuk;Kwak, Jae-Gun;Park, Chun-Soo;Lee, Jeong-Ryul;Kim, Yong-Jin
    • Journal of Chest Surgery
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    • v.42 no.4
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    • pp.418-425
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    • 2009
  • Background: Cardiopulmonary bypass (CPB) involves use of an initial priming volume which can cause side effects such as hemodilution, transfusion, inflammatory reaction and edema. Hence, there have been efforts made tore-duce the initial priming volume. We compared this traditional method to a CPB method that uses a minimized priming volume (MPV). Material and Method: For 97 patients who underwent congenital cardiac surgery between July 2007 to June 2008, we discussed each case and decided which method to use. We reviewed the medical records and cardiopulmonary bypass sheets of the patients. Result: We used a MPV method for 46 patients, and a traditional method for the other 51. There were no significant differences in preoperative and intraoperative characteristics between the two groups, such as body weight, age, cardiopulmonary bypass time, lowest body temperature, etc. However, the priming volume was much smaller in the MPV group than the traditional group (p<0.001). The volume of initially mixed packed RBC was also much smaller in the MPV group (p<0.001). There were no significant differences in postoperative mortality and neurologic complications. Conclusion: We could significantly reduce the initial priming volume and initially mixed pRBC volume with the revised CPB method. We suggest that this method be used more widely for congenital cardiac surgery.

Effect of Seed Priming and Pellet Coating Materials on Seedling Emergence of Aster koraiensis (프라이밍과 펠렛코팅 소재가 벌개미취 종자의 유묘 출현율에 미치는 영향)

  • Kang, Won Sik;Kim, Min Geun;Kim, Soo Young;Han, Sim Hee;Kim, Du Hyun
    • Journal of Korean Society of Forest Science
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    • v.109 no.1
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    • pp.41-49
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    • 2020
  • In this study, the effect of seed pre-treatments and pellet coating materials to enhance the efficiency of large-scale propagation of Aster koraiensis seeds were investigated. Seeds were immersed in water for one day, and only those that sank were used for pre-treatment to use filled seeds. Pre-treatments were divided into hormone treatments, with gibberellic acid (GA3; 200 and 500 ppm) and 24-epibrassinolide (10-6, 10-7, and 10-8M), and priming with potassium nitrate (100 mM of KNO3). To produce pellet-coated seeds, pellet materials (DTCS or DTK) were applied to control (unprimed) and primed seeds with binders (PVA or CMC). The maximum germination percent (GP) of seeds before pellet coating was 65% (with the priming treatment), and there was no difference in the GP of seeds among hormone treatments. For seeds sown in a growth chamber on filter paper, GP was 41% for control (unprimed/uncoated) seeds, 65% for uncoated primed seeds, 71% for DTCS/PVA-pellet-coated seeds, and 42% for DTK/CMC-pellet-coated seeds. Seeds that were primed first and then pellet-coated showed greatly improved GP, mean germination time (MGT), and germination rate than seeds that were only pellet-coated. For seeds sown in commercial soil in a greenhouse, control seeds had a GP of 27%, whereas primed seeds had the highest GP (58%), and their MGT and GT were 9.4 days and 7.0%·day, respectively. In addition, DTK/PVA-pellet-coated seeds (40%) also had a GP higher than the control (27%), and their MGT was 15-27 days. For seeds sown in sandy-loam soil in a greenhouse, unprimed-pellet-coated seeds and primed-pellet-coated seeds both had GPs ranged of 39%, which were lower than that of control seeds. In general, the seeds that were pellet-coated with DTK had a higher GP than those pellet-coated with DTCS. Furthermore, the MGT of unprimed-pellet-coated seeds was 15.0-19.8 days, which was longer than the MGT of primed-pellet-coated seeds. These results suggest that priming enhances seedling emergence of Aster koraiensis seeds. Moreover, when priming is combined with pellet coating, DTK is a more suitable pellet material than DTCS, and PVA and CMC are equally suitable adhesives.

Adequate Heparin-protamine Neutralization on using Blood Cardioplegic Solution during Extracorporeal Circulation (체외 순환시간의 경과에 따른 Heparin과 Protamine의 적정량에 관한 연구)

  • 변형섭
    • Journal of Chest Surgery
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    • v.21 no.2
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    • pp.203-210
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    • 1988
  • The clinical experience with the activated clotting time[A.C.T.] for the control of heparin and protamine therapy during cardiopulmonary bypass in 40 patients between April, 1987 and September, 1987 is reviewed retrospectively. All of patients used with cold blood potassium cardioplegia for myocardial protection under standard cardiopulmonary bypass, priming and perfusate techniques respectively. This study was divided into 2 groups of patients followed by cardiopulmonary bypass time. Twenty patients, within 60 minutes of cardiopulmonary bypass time[group A] were compared with twenty patients, from 60 to 120 minutes of cardiopulmonary bypass time[group B]. Using blood cardioplegia for myocardial protection, Author observed wide variation of A.C.T. in individual response to initial heparinization[2mg /kg] and no requirement of additional heparin during cardiopulmonary bypass until 120 minutes. Total heparin amount during cardiopulmonary bypass was not related to body weight and body surface area in the both groups. After cardiopulmonary bypass, amounts of protamine for neutralization of heparin were more required in group B.

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Priming time and etching effect on shear bond strength of self-etching adhesive (프라이머 처리시간과 산부식이 자가산부식 접착제의 접착강도에 미치는 영향)

  • Kang, In-Joo;Park, Jeong-Won
    • Restorative Dentistry and Endodontics
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    • v.29 no.2
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    • pp.185-190
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    • 2004
  • 자가산부식 접착제 (self-etching primer)는 법랑질과 상아질을 동시에 산부식과 프라이머 처리를 함으로 임상 시술 시간을 단축시킬 뿐만 아니라, 임상과정 중 발생할 수 있는 술자의 실수 및 타액등에 의한 오염의 가능성을 줄일 수 있는 장점을 가지고 있다. 그러나 약산을 이용한 산부식법이 법랑질에 대해 탈회효과 및 접착강도에 있어 논란이 되고 있다. 이에 본 연구에서는 인산을 이용한 추가적인 산부식 및 자가산부식 접착제의 적용시간을 달리할 경우 법랑질에 대한 접착강도의 변화를 알아보고자 하였다. 135개의 발거한 우치의 법랑질 면을 #600사포로 연마한 후 9개의 군으로 분류하였다. 각 군은 부가적인 32% 인산을 처리하지 않은 군 (1~3군), 15초간 처리한 군 (4~6군), 60초간 처리한 군 (7~9군)으로 나누고, 이를 다시 Clearfil SE Bond의 프라이머로 5초 (1, 4, 7군), 20초 (2, 5, 8군), 60초 (3, 6, 9군)간 처리한 군으로 분류하였다. 접착제 처리한 면에 Clearfil AP-X 복합레진을 접착하고 24시간 경과 후 전단응력 강도를 측정하였다. 결과는 One-way ANOVA 처리후 Duncan's multiple range test로 사후검증하였다. 동일한 자가산부식 프라이머 처리 시간을 가진 군에서 산 부식을 한 경우가 그렇지 않은 군에 비해 높은 접착강도를 나타내었으며 인산부식 시간의 차이에 의한 영향은 없었다.(1 < 4, 7군/ 2 < 5, 8군/ 3 < 6, 9군). 자가산부식제의 프라이머 적용 시간에 의한 효과는 1군(5초 적용)을 제외한 나머지 군의 경우 동일한 인산 처리군에서는 차이가 없는 것으로 나타났다.(1군 < 2, 3군/ 4=5=6군/ 7=8=9군). Clearfil SE Bond 접착제의 법랑질에 대한 접착강도는 부가적인 산부식을 통하여 증가시킬 수 있으며 프라이머의 적용 시간에 의한 효과는 제조자의 지시에 의한 시간 이상 적용할 경우 차이가 없는 것으로 나타났다.