Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Leakage of Seed Reserve Nutrient in Artificially Aged Pepper Seeds and Enhancement of Seed Vigor by Priming

노화처리된 고추종자의 저장성분 누출과 priming 처리가 종자활력 증진에 미치는 영향

  • Kang Jum-Soon (School of Bio-Resources, Pusan National University) ;
  • Choi In-Soo (School of Bio-Resources, Pusan National University)
  • 강점순 (부산대학교 생명자원과학부) ;
  • 최인수 (부산대학교 생명자원과학부)
  • Published : 2006.04.01
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Abstract

Quantity of protein, amino acid, and sugar leaked from seeds was greater as the viability of seeds was dropped by the time elapsed of seed aging treatment. In the seeds with the artificial aging treatment for 20 days, 35.8 mg of protein was leaked on the 4th day after soaking, which was 6.9 times higher than that of control. Leakage of amino acid was also higher from low quality seeds treated with the aging treatment. In the seeds with the aging treatment for 20 days, $36.5{\mu}g$ fig of sugar was leaked on the 4th day after soaking, which was 2.8 times higher than that of control. The leakage of inorganic compound was higher from the low quality seeds, and leakage of total protein, amino acid, and sugar. According to .the quantity of leakage, water soluble compounds, which can be used for the assessment of seed quality without any destruction, were protein and potassium. Germination rate and percentage of seeds were dropped with the seed aging treatment, and the seed viability could be recovered by priming treatment. This phenomenon was very clear when the low quality seeds were germinated at low temperature.

노화처리 일수가 경과하여 활력이 저하된 종자일수록 종자에서 누출되는 단백질, 아미노산, 가용성 당의 누출량이 많았다. 20일간 노화처리된 종자는 침지 4일째 종자 1g당 35.8 mg의 단백질이 누출되어 건전종자에 비해 6.9배 많았다. 아미노산 누출량도 노화처리된 저활력 종자에서 증가하였다. 가용성 당의 누출량도 20일 노화처리된 저활력 종자에서 침지 4일째 종자 1g당 $36.5{\mu}g$이 누출되어 건전종자보다. 2.8배 많았다. 무기성분의 누출량은 저활력 종자에서 많았으며 무기성분의 누출량은 단백질, 아미노산 및 가용성 당보다는 적었다. 누출량에 근거하여 비파괴적으로 종자활력을 추정할 수 있는 가용성 물질은 단백질과 $K^+$였다. 노화처리된 종자는 발아율과 발아세가 저하되었으며, 종자활력이 저하된 노화종자를 priming 처리함으로써 종자활력을 회복시킬 수 있었다. 이러한 현상은 저활력 종자를 저온에서 발아시킨 경우에 더욱 뚜렷하였다.

Keywords

References

  1. Abdul-Bake, A. A. and J. D. Anderson. 1970. Viability and leaching of sugars from germinating barley. Crop Sci. 10, 31-32 https://doi.org/10.2135/cropsci1970.0011183X001000010012x
  2. Argerich, C. A., K. J. Bradford and A. M. Tarquis. 1989. The effects of priming and ageing on resistance to deterioration of tomato seeds. J. Expl. Bot. 40, 593-598 https://doi.org/10.1093/jxb/40.5.593
  3. Bradford, M.M. 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  4. Coolbear, P., A. Francis and D. Grierson. 1984. The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. J. Exp. Bot. 35, 1609-1617 https://doi.org/10.1093/jxb/35.11.1609
  5. Dearman, J., P. A. Brocklehurst and R. L. K. Drew. 1986. Effects of osmotic priming and aging on onion seed germination. Ann. Appl. Biol. 108, 639-648 https://doi.org/10.1111/j.1744-7348.1986.tb02003.x
  6. Fu, J. R., X. H. Lu, R. Z. Chen, B. Z. Zhang, Z. S. Liu, Z. S. Li and D. Y. Cay. 1988. Osmoconditioning of peanut( Arachis hypogea L.) seeds with PEG to improve vigor and some biochemical activities. Seed Sci. Technol. 16, 197-212
  7. Hong, S. B. and S. S. Lee. 1995. Sugar leakage from differently- aged seeds of rape, chinese cabbage and radish. Kor. J. Crop Sci. 40, 322-327
  8. ISTA. 1985. International rules for seed testing. International Seed Testing Association. Seed Sci. Tech. 13, 299-355
  9. Kauufaman, G. 1991. Seed coating: A tool for stand establishment: a stimulus to seed quality. HortTechnol. Oct./Dec, 99-102
  10. Khan, A. A. 1992. Preplant physiological seed conditioning. Hort. Rev. 13, 131-181
  11. Lee, S. S. and S. B. Hong. 1995. Nondestructive vigor test of seed. Kor. J. Crop Sci. 40, 314-321
  12. Lee, S. S. and S. B. Hong. 1995. Leakage of sugars, amino acid and protein from differently-aged seeds of sesame, welsh onion and lettuce. Kor. J. Crop Sci. 40, 407-412
  13. Lee, S. S. and S. B. Hong. 1997. Leakage of organic compound from different seed qualities of onion, welsh onion, and leaf lettuce varieties. J. Kor. Soc. Hort. Sci. 38, 625-628
  14. Lee, S. S., S. B. Hong and M. K. Kim. 1997. Nondestructive seed viability test chinese cabbage and radish varieties by sinapine leakage. J. Kor. Soc. Hort. Sci. 38, 498-501
  15. Loomis, E. L. and O. E. Smith. 1980. The effect of artificial aging on the concentration of Ca, Mg, Mn, K, and Cl in imbibing cabbage seed. J. Amer. Soc. Hort. Sci. 105, 647-650
  16. McCready, R. M., J. Guggolz, V. Silviera and H. S. Owens. 1950. Determination of starch and amylose in vegetables. Anal. Chem. 22, 1155-1158
  17. Simon, E. W. and R. M. Raja-Harun. 1972. Leakage during seed imbibition. J. Exp. Bot. 23, 1076-1085 https://doi.org/10.1093/jxb/23.4.1076
  18. Taylor, A. G., D. B. Churchill, S. S. Lee, D. M. Bilsland and T .M. Cooper. 1993. Color sorting of coated Brassica seeds by fluorescent sinapine leakage to improve germination. J. Amer. Soc. Hort. Sci. 118, 551-556
  19. Taylor, A. G., S. S. Lee, M.M. Beresniewicz and D. H. Paine. 1995. Amino acid leakage from aged vegetable seeds. Seed Sci. Technol. 23, 113-122
  20. Woodstock, L. M., K. Furman and H. R. Leffler. 1985. Relationship between weathering deterioration and germination, respiratory metabolism, and mineral leaching from cotton seeds. Crop Sci. 25, 459-466 https://doi.org/10.2135/cropsci1985.0011183X002500030008x
  21. Woodstock, L. W. and K. J. Tao. 1981. Prevention of imbibitional injury in low vigor soybean embryonic axes by osmotic control of water uptake. Physiol. Plant 51, 133-139 https://doi.org/10.1111/j.1399-3054.1981.tb00891.x
  22. Yemm, E. W. and E. C. Cocking. 1955. The determination of amino acids with ninhydrin. Analyst 80, 209-213 https://doi.org/10.1039/an9558000209

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