• 대한소아치과학회지
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• 제33권2호
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• pp.323-328
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• 2006

상아질 형성부전증은 상아질 형성 이상이 초래되는 유전성 질환으로, 주로 상염색체 우성으로 유전된다. 8000명당 1명꼴로 발생하며 남녀 성별의 차이가 없고 유치열과 영구치열 모두에서 나타난다고 알려져 있으며, Shield등은 세 가지 유형으로 분류하였다. 이환 받은 치 아는 다양한 치아 변색, 법랑질의 박리, 급속도의 상아질의 파괴, 심한 마모현상 등의 임상소견을 보인다. 따라서 치간이개, 고경감소, 저작능력의 저하, 치태와 치석의 침착 등이 나타날 수 있다. 방사선학적 소견으로 치경부 수축 구근 상치관, 짧고 가는 치근, 치수강과 근관의 폐색, 건전치 에서 치근부 병변 등이 보인다. 상아질 형성부전증에 대한 치료 목표는 심미성 회복, 저작능력회복, 교합고경의 회복을 들 수 있다. 본 증례들은 치아의 마모와 변색을 주소로 경북대학병원 소아치과에 내원한 두 명의 환아들에서 임상 및 방사선학적 검사 소견상 상아질 형성 부전증으로 판단되어 환아의 손상된 치아에 대해 치료를 시행하여 기능적 심미적으로 만족할 만한 결과를 얻어 이를 보고하는 바이다.

• Restorative Dentistry and Endodontics
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• 제24권1호
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• pp.100-115
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• 1999

The purpose of this study was to investigate the distribution of calcitonin gene-related peptide containing nerve fibers in rat pulp after dentinl injury by means of immunohistochemistry and confocal laser scanning microscope. The Spague-Dawley rats weighing about 250-300gm were used. The animals were devided into normal control and experimental groups. Experimental animals were sacrified 1, 2, 4, 7, 10, 21days after dentinal injury (dentin cutting, and then acid etching with 35% phosphoric acid) on the maxillary molar teeth. The maxillary teeth and alveolar bone were removed and immersed in the 4% paraformaldehyde in 0.1M phosphate buffer (pH 7.4), then were decalcified with 15% formic acid for 10 days. Serial frozen $50{\mu}m$ thick sections were cut on a cryostat. The rabbit CGRP antibody was used as a primary antibody with a dilution of 1:2000 in 0.01M PB. The sections were incubated for 48 hours at $4^{\circ}C$, and placed into biotinylated antirabbit Ig G as a secondary anti body with dilution of 1:200 in 0.01M PB and incubated in ABC(avidin-biotin complex). The peroxidase reaction was visualized by incubating the sections in 0.05% 3,3 diaminobenzidine tetrahydrochloride containing 0.02% $H_2O_2$. For the confocal laser scanning microscopic examination, Primary antibody reaction was same as immunoperoxidase stainning, but fluorescein isothiocyanate(FITC)-conjugate antirabbit IgG as a secondary antibody was used. The confocal laser scanning microscope was used for the examination. A series of images of optical sections was collected with a 20x objective at $3{\mu}m$ intervals throughout the depth of specimen. FITC fluerescence was registrated through a 488nm and 568nm excitation filter, and images were saved on optical disk. The stereoscopic images and three dimentionnal images were reconstructed by computer software, and then were analyzed. The results were as follows : 1. In normal control group, CGRP containing nerve fibers were coursed through the root with very little branching, and then formed a dense network of terminals in coronal pulp. 2. A slight increase in CGRP containing nerve fibers at 1 and 2day postinjury was noted subjacent to the injury site. In the 4day group, there were an extensive increase in the number of reactive fibers, followed by a partial return toward normal levels at 7~10 day postinjury, and return by 21days. 3. The sprouting of the CGRP containing nerve fibers was evident within 2day after dentinal injury, and by 4days there was a maximal increased, but was decreased at 7days and returned to normal 10~21 day postinjury. 4. In confocal laser scanning microscopic exammination, the distinct distribution pattern and sprouting reaction of CGRP containing nerve fibers were observed in stereoscopic images and three dimentional images. These results suggest that CGRP containing nerve fiber can be important role in the response to dental injury and pain regulation.