• KSBB Journal
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• v.11 no.1
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• pp.22-29
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• 1996

Immobilized bioprocess was carried out for continuous production of cyclosporin A (CyA) produced intracellularly as a secondary metabolite by a filamentous fungus, Tolypocladium inflatum. Immobilization procedure for entrapping conidiospores of the producer was significantly simplified by use of a modified immobilization technique. A newly-designed immobilized perfusion reactor system (IPRS) showed good process benefits as demonstrated by the role of the high density immobilized cells as an efficient biomass generator, continuously supplying highly active CyA-producing free cells (1.0g/$\ell$/hr) even at very high dilution rate ($0.1hr^{-1}$). IPRS bioprocess was possible since efficient decantor system developed in our laboratory separated the sloughed-off free cells from the immobilized biomass effectively, thus overcoming wash-out phenomenon frequently encountered in continuous free cell cultures. Furthermore the released-free cells remaining in the bulk solution did not appear to cause substrate mass transfer limitation which was often experienced in suspended mycelial fungal cell fermentations. The primary reason for this was that the suspension broth of the IPRS mainly consisted of roundshaped short mycelial fragments and conidiospores, still remaining Newtonian even at high cell density. In parallel with IPRS bioprocess development, other key factors to be considered necessarily for significant increase in CyA productivity would be strain improvement and medium optimization for the immobilized cells.

• Journal of Aquaculture
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• v.20 no.4
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• pp.232-238
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• 2007

The distribution of stock density and larvae of Atrina pectinata was examined in Deukryang Bay. Sampling of adult stock was seasonally conducted from 29 stations in the bay in May, August, and November 1995 and February 1996. And the larvae were sampled with interval of 3 to 7 days from 12 stations from July to October 1996. Seasonal abundance of the adult in number and in biomass were the highest in August ($18\;inds./m^2$) and in November ($2,790\;g/m^2$), respectively, at G1 station located in the most inner bay. In frequency distribution of shell height, the mode in May appeared in 10-14 cm group with 62%, and that of the other seasons in $15{\sim}19\;cm$ group. The larvae were also distributed mainly at G1 as the adult were. The larval abundance showed a periodical cycle which seemed to be related to lunar cycle. The highest abundance of the larvae occurred on August 16 with $8\;inds./m^3$, and then decreased continuously. With regard to frequency distribution of larval shell height, the larvae less than $200\;{\mu}m$ occurred from July to October. And the mode in July appeared in small size group ($200{\sim}249\;{\mu}m$) with 38%. These results mean that A. pectinata spawned from July to October, mainly middle July to middle August. The larval distribution showed the same geographical tendency to adult distribution. Both the adult and the larvae mainly distributed in the most inner bay and west area of Deukryang island, where was shallower, warmer, and higher primary productivity than the entrance area of the bay and east area of the island. However, larval abundance of A. pectinata in this study was so low compared to adult biomass. The reason for this unusual phenomenon should be understood with the detail research in future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.10
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• pp.361-371
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• 2020

Since the implementation of the sunset law in 2020, concerns have been raised over the reckless development of long-term non-executed urban parks. In this study, the FSDAF method and CASA-NPP model were used to evaluate the annual average NPP of long-term non-executed urban parks in Seoul. Based on this, the carbon loss and economic value were assessed under five development scenarios. The total NPP value of long-term non-executed urban parks, except for the greenbelt area in Seoul, was 4,892.18 t C. In the first scenario, the NPP and cost were 4,892.18 t C of vegetation carbon and 1.18 billion won, 2,548.55 t C of vegetation carbon and 615 million won in the second scenario, 238.94 t C of vegetation carbon and 58 million won in the third scenario, 848.38 t C of vegetation carbon and 205 million won in the fourth scenario, and 1,596.00 t C of vegetation carbon and 385 million won in the fifth scenario. These results are meaningful for evaluating vegetation carbon and economic value loss according to five different development scenarios. The results of this study are expected to be useful for the preparation of measures to minimize the impact of the development of long-term non-executed urban parks.

• Applied Biological Chemistry
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• v.14 no.3
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• pp.191-200
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• 1971

As a series on the soy-bean protein and their related substances 9 samples were collected from 9 places such as straws (Rice) to obtain bacterial strains which produce protease. From these samples total of 23 strains were isolated by the use of dilution pour plate method. For all isolated strains primary screening of productivity of protease was performed and useful straines with regard to protease productivities were identified. Optimum conditions for enzyme action of protease from isolates $D_9$, $F_{20}$ strains were pH 7.5 and $40^{\circ}C$. Chung-Kook-Jang is one of the characteristic foods in Korea made from soy-bean by fermentation. The chief bacterium is Bacillus subtilis and the chief change which takes place in soy-bean during fermentation is degradation of protein. Three kinds of Chung-Kook-Jang were prepared using three different strains of Bacillus natto, $D_9\;and\;F_{20}$ from isolated. Water soluble-N, TCA soluble-N, amino-N and peptide-N were measured about the steamed soybean, Chung-Kook-Jang prepared with three strains of bacteria. Water soluble-N decreased very largely in steamed soybean, but in Chung-Kook-Jang it increased to 85% of raw soy-bean.

• Korean Journal of Agricultural and Forest Meteorology
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• v.9 no.4
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• pp.247-259
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• 2007

In this study, we examined the flux of carbon and water using an eco-hydrological model, Regional Hydro-Ecologic Simulation System (RHESSys). Our purposes were to develop a set of parameters optimized for a well-designed experimental watershed (Gwangneung Research Watershed, GN) and then, to test suitability of the parameters for predicting carbon and water fluxes of other watershed with different regimes of climate, topography, and vegetation structure (i.e Gangseonry Watershed in Mt. Jumbong, GS). Field datasets of stream flow, soil water content (SWC), and wood biomass product (WBP) were utilized for model parameterization and validation. After laborious parameterization processes, RHESSys was validated with the field observations from the GN watershed. The parameter set identified at the GN watershed was then applied to the GS watershed in Mt. Jumbong, which resulted in good agreement for SWC but poor predictability for WBP. Our study showed that RHESSys simulated reliable SWC at the GS by adjusting site-specific porosity only. In contrast, vegetation productivity would require more rigorous site-specific parameterization and hence, further study is necessary to identify primary field ecophysiological variables for enhancing model parameterization and application to multiple watersheds.

• Korean Journal of Ecology and Environment
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• v.43 no.4
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• pp.492-502
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• 2010

The study objects were to analyze long-term and seasonal variations of nutrients (N, P), suspended solids, N:P ratios, algal chlorophyll, and trophic state along with general water quality parameters in four sampling sites including two intake tower sites supplying drinking water in Daechung Reservoir. For the analysis, we used water quality long-term data sampled during 1998~2007 by the Ministry of Environment, Korea. Interannual and seasonal trends in inflow and discharge near the intake tower facilities over the ten years were directly influenced by rainfall pattern. The distinct difference between wet year (2003) and dry year (2001) produced marked differences in water temperature, pH, dissolved oxygen, organic matter contents, nutrients, and these variables influenced algal biomass and trophic state. Values of TP varied depending on the year and locations sampled, but monthly mean TP always peaked during July~August when river inflow and precipitation were maxima. In contrast, TN varied little compared to TP, indicating lower influence by seasonal flow compared to phosphorus. The number of E. coli were highest in Site 2 (Chudong intake tower) and varied largely, whereas at other sites, the numbers were low and low variations. Contents of chlorophyll-${\alpha}$ (CHL), as an estimation of primary productivity, varied largely depending on the year and season. The maximum of CHL occurred at Muneu intake tower (S4) during 2006 when the precipitation and inflow were lowest. In contrast, another CHL peak was observed in Site 2 (Chudong intake tower) in 2006 when one of the largest typoons (Ewinia) occurred and river runoff were maximum. So the CHL maxima were associated with both wet year (high flow, high nutrient supply) and dry year (low flow, nutrient supply by littoral zone). Such conditions influenced trophic states, based on Trophic State Index of nutrients and CHL. Based on all analyses, we can provide some clues for management and protection strategies of two intake tower sites.

• Journal of Plant Biology
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• v.24 no.1
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• pp.1-12
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• 1981

Phosphorus dynamics in terms of specific absorption rate, inflow and outflow rates. turnover rate, demand and supply, and utility index of a high yield Zea mays L. cv. Bokgyo field were evaluated using an analysis of successive production structures. The analysis was adopted for measuring quantitative changes in the population by stratified clip technique on every two weeks during the growing season. The seasonal trends of specific absorption rate (2. 4 mg P/g/day in maximum) and specific absorption efficiency (0. 03) closely correlated with that of relative growth rate of the population. The overall inflow and outflow of phosphorus was 3.41 g P/$m^2$/yr showing the maximum inflow of 2.99 g P/$m^2$/month in July. While the maximum phosphorus standing crop was 1.4 g P/$m^2$ showing the maximum turnover rate of 178% in late June. The accumulation of phosphorus along plant height declined monotonically in stems and roots but increased in foliage after heading. The proportions of the total annual demand of phosphorus were 24.4% for leaves, 22.5% for stems, 49.6% for fruits and 3.5% for roots. These demands were met with internal (18.2 %) and external (81.8 %) supplies. The seasonal highest phosphorus utility index was 1,091 in early June, while the average value was 655.

• Magazine of the Korean Society of Agricultural Engineers
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• v.21 no.2
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• pp.28-36
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• 1979

One of most important problems in the Monsoon Asia today is the production of rice paddy to meet the needs of the ever increasing population. Diversemeans are being employed to meet this demand, both by increasing productivity of existing farm land and by bringing further areas into cultivation. The primary step in either field is to ensure that there is sufficient moisture in the soil to suit the paddy, and at the same this means that excess moisture has to be drained off the land, while in others irrigat ion has to be employed to bring sufficient water to an area. In view of the fact that the project comprises a huge amount of earthwork, it can be carried out by extensive use of construction machinery in order to shorten the period. As farm ditch has a comparatively small section with shallow cutting depth, inaddition, there is lack of access road in the field, the excavation equipment with bulldozer or tracter-shovel (backhoe) type are not applicable because there are mostly adapted for the excavation of deep and wide section. Mini-backhoe with its bucket width not larger than 0. 3m, and width of blade not larger than 1. 00m seems to be more adaptable. About 80% of excavation of ditch section will be done by the machinery while the other 20% of excavation together with the finishing of the section are supposed to be done by man-power. The embankment of ditch section can be compacted by the crawler of backhoe when it is moving along the ditch for excavation. However, Lowland paddy field in the Monsoon Asia are made particulary in rain season, therefore, heavy machinery is not easy excavation for ditch. It is very important to know exact ground support power of the working site and select machines with corresponding ground pressure. Ground support power is variable subject to quality and water content of soil and therefore selection of machines should be made duly considering ground condition of the site at the time of construction works. Farm ditches dug and compacted by mannual labar are of poor quality and subject to destruction after one or two years of operation. On the other hand, excavation and compaction by bulldozer is not practical for ditches. Backboe is suitable for slope land, but this is required cycle time of bucket excavation and dumped out. If a small-scale farm ditch trencher adaptable to lowland paddy field is invented, such a machine could greatly accelerate the massive construction work envisaged in many countries and thus significantly speed up the most difficult part of irrigation development and management in Monsoon Asia.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.596-604
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• 2019

This study conducted a sediment culture experiment to investigate the effects of sediment oxygen demand (SOD) and environmental factors on sediment and water quality. We installed a leaching tank in the laboratory, cultured it for 20 days, and analyzed the relationship between P and Fe in the sediment. As a result, the dissolved oxygen of the water layer decreased with time, while the oxidation-reduction potential of the sediment progressed in the negative direction to form an anaerobic reducing environment. The SOD was measured to be 0.05 mg/g at the initial stage of cultivation and increased to 0.09 mg/g on the 20th day, indicating the tendency of increasing consumption of oxygen by the sediment. The change is likely to have caused by oxygen consumption from biological-SOD, which is the decomposition of organic matter accumulated on the sediment surface due to the increase of chl-a, and chemical-SOD consumed when the metal-reducing product produced by the reduction reaction is reoxidized. The correlation between SOD and causality for sediment-extracted sediments was positive for Ex-P and Org-P and negative for Fe-P. The analysis of the microbial community in the sediment on the 20th day showed that anaerobic iron-reducing bacteria (FeRB) were the dominant species. Therefore, when the phosphate bonded to the iron oxide is separated by the reduction reaction, the phosphate is eluted into the water to increase the primary productivity. The reduced substance is reoxidized and contributes to the oxygen consumption of the sediment. The results of this study would be useful as the reference information to improve oxygen resin.

• Korean Journal of Environmental Biology
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• v.36 no.4
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• pp.610-619
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• 2018

This study was carried out in August and September 2016 to investigate the species composition and biomass of benthic diatoms in Yubu Island, Gomso Bay, Imja Island, and Yeoja Bay tidal flat of Southwestern coast. There were 181 species of 48 genera in Yubu Island tidal flats, 194 species of 51 genera in Gomso Bay tidal flats, 224 species of 64 genera in Imja Island tidal flats and 188 species of 56 genera in Yeoja Bay tidal flats. A total of 274 species of 70 genera appeared. Pennales appeared more widely than Centrales and were dominated by Paralia sulcata and Navicula spp. Biomass of Yubu Island tidal flat ranged from $18.8-136.1mg\;m^{-2}$, $31.9-215.7mg\;m^{-2}$ in Gomso Bay, $2.9-120.2mg\;m^{-2}$ in Imja Island and $10.1-147.7mg\;m^{-2}$ in Yeoja Bay. The range of total biomass from 4 areas was $2.9-215.7mg\;m^{-2}$. The concentration of phaeopigment and degradation product of chlorophyll-a was $1.7-470.8mg\;m^{-2}$ in Yubu Island tidal flat, $52.3-277.2mg\;m^{-2}$ in Gomso Bay, $0.6-78.9mg\;m^{-2}$ in Imja Island and $39.1-346.3mg\;m^{-2}$ in Yeoja Bay. Compared with the results reported in this study area and the domestic tidal flats, it cannot be directly compared and evaluated due to various factors such as the timing of the survey, the frequency of the survey, the analysis method and geography. The southwestern coast of Korea has a variety of benthic diatoms and the high concentration of chlorophyll-a is the main determinant of primary productivity.