• 제목/요약/키워드: Primary Culture

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Liminal but Competent: Latin American Migrant Children and School in Australia

  • Amigo, Maria Florencia
    • Child Studies in Asia-Pacific Contexts
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    • 제2권1호
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    • pp.61-75
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    • 2012
  • Indisputably school is the main institution that socialises migrant children into the culture of their new country. Through school they learn the new language and customs, which will enable them to become cultural brokers between the new cultural world and their families. During this process migrant children often transit a liminal terrain where their roles and identity become at the same time diverse and ambivalent. Despite the challenges involved these children often become experts in juggling different cultures, environments and expectations. This study explores the experiences of Latin American children and their families as they start primary school in Australia.

ANTAGONISTIC EFFECTS OF INTERFERONS (INFs) AND SODIUM ORTHOVANADATE ON RESPONSES PRODUCED BY TCDD IN SEVERAL CULTURE SYSTEMS

  • Kim, Hwan-Mook
    • Toxicological Research
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    • 제7권2호
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    • pp.239-255
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    • 1991
  • Several types of IFNs were tested for their ability to suppress TCDD-inducible P-450 dependent mixed function oxidase (MFO) system in mouse primary hepatocytes. Mouse IFN-gamma (IFN-G) markedly suppressed EROD activity when added at the same time as TCDD (10 nM). The antagonism of EROD activity by IFN-G exhibited both a dose-(10-100 U/ml) and time-depedence. In contrast, mouse IFN-A/B was only moderately suppressive and only at high concentrations (500 U/ml). Rat IFN-G was even more selective than mouse, wherase human IFN-G had no activity.

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고위험병원체 결핵균의 신속진단을 위한 액체배양시스템 평가 (Evaluation of Liquid Culture System in Sputum Culture and Drug Susceptibility of Mycobacterium tuberculosis)

  • 김진숙;김승철;전보영;박승규
    • 미생물학회지
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    • 제45권3호
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    • pp.281-285
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    • 2009
  • 본 연구에서는 객담 내 결핵균의 배양시험과 약제 감수성 시험 방법으로서 BacT/Alert 액체 배지, Ogawa 배지 및 $L\ddot{o}wenstein$-Jensen 배지 배양법을 비교하였다. 신속 액체배양시스템은 기존의 Egg-based media (Ogawa, $L\ddot{o}wenstein$-Jensen 배지)에 비해 높은 민감도와 빠른 배양으로 결핵환자의 초기 객담 검사와 약제 감수성 시험에 널리 사용되어 왔다. 객담은 N-Acetyl-L-cystine과 4% NaOH로 처리한 후 BacT/Alert 액체 배지 시스템과 Ogawa 배지에 접종하고 배양하여 비교하였다. 결핵환자 객담 검체 135개 중 도말검사결과 양성은 95개였고, Ogawa 배지 배양결과 양성으로 확인된 수는 89개(65.9%)였으며, BacT/Alert 액체 배지에서는 97개(71.9%)가 양성으로 확인되었다. 결핵균 배양 양성 검체의 평균배양일은 Ogawa 배지에서 22.4일, BacT/Alert 액체 배지는 11.3일로 단축되는 것을 확인하였다. 약제 감수성 시험에는 32개 결핵균주를 이용하였으며, isoniazid를 첨가한 BacT/Alert 액체 배지와 $L\ddot{o}wenstein$-Jensen 배지에서의 약제 감수성 시험 검사결과 일치률은 87.5%를 보였고, rifampicin에서의 일치률은 90.6%로 나타났다.

시큐리티조직의 조직문화와 임파워먼트 및 조직애착도의 관계 (Relation of Empowerment and Organization Attachment with the Organization Culture of Security Organization)

  • 김찬선
    • 융합보안논문지
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    • 제14권1호
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    • pp.33-41
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    • 2014
  • 이 연구의 목적은 시큐리티조직의 조직문화와 임파워먼트 및 조직애착도의 관계를 규명하는데 있다. 이 연구는 2012년 수도권소재(서울) 시큐리티회사에서 재직하고 있는 시큐리티요원들을 모집단으로 설정한 후 유의표집법을 이용하여 최종분석에 이용된 사례 수는 총 280명이다. 분석방법은 SPSSWIN 18.0을 이용하여 빈도분석, 요인분석, 신뢰도분석, 다중회귀분석, 경로분석 등을 실시하였다. 설문지의 신뢰도는 Cronbach's ${\alpha}$값이 .690이상으로 나타났다. 결론은 다음과 같다. 첫째, 시큐리티조직의 조직문화는 임파워먼트에 영향을 미친다. 즉, 합리개발적문화가 잘 이루어질수록 역량자기결정력, 의미성, 영향력은 증대된다. 또한, 합의적문화가 잘 이루어질수록 역량자기결정력은 높아지며, 위계적문화가 잘 이루어질수록 의미성은 증대된다. 둘째, 시큐리티 조직의 조직문화는 조직애착도에 영향을 미친다. 즉, 위계적문화가 잘 이루어질수록 지속규범적애착은 증대된다. 또한, 합리개발적문화가 잘 이루어질수록 정서적애착은 높아진다. 셋째, 시큐리티조직의 임파워먼트는 조직애착도에 영향을 미친다. 즉, 역량자기결정력이 부족할수록 지속규범적애착도는 감소하는 것으로 나타났다. 반면, 의미성 및 영향력을 증대시켜 줄수록 지속규범적애착도는 높다. 또한, 의미성을 증대시켜 줄수록 정서적애착도는 증가한다. 넷째, 시큐리티조직의 조직문화는 임파워먼트 및 조직애착도에 직 간접적인 영향을 미친다. 즉, 임파워먼트는 조직문화와 조직애착도를 매개하는 중요한 변수이다.

우리나라의 소리문화 창조를 위한 사운드스케이프 디자인 운동의 전개와 실천 전략 (The Practical Strategies and Developments of Soundscape Design Movement for Creation of Sound Culture in Korea)

  • 한명호;오양기;황혜주
    • KIEAE Journal
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    • 제8권5호
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    • pp.35-41
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    • 2008
  • The aim of this study is to find out directions for development of soundscape design for creating a unique sound culture in a region, and to search for the practical strategies toward a soundscape design movement in Korea. For these specific aims, several components in soundscape through considering from original conception and ideology of soundscape, such as the concept of soundscape, the meaning of soundscape design, the categories of soundscape researh : keynote sounds, sound signals, and soundmarks, and the primary problems and strategies in the world soundscape project(WSP) are investigated. The result shows that it is necessary to practice several ways in our housing life, that is, the recovery of our coenesthesia, development of the residential environments considering the soundscape, revitalization of community consciousness, rehabilitation of the sensibility, and identity excavation of sound culture, and it is very important to spread out a plan for integrating the following four categories of strategies : conceptual, educational, political, and social.

Job Performance During COVID-19 Pandemic: A Study on Indonesian Startup Companies

  • MUTTAQIN, Galih Fajar;TAQI, Muhammad;ARIFIN, Bustanul
    • The Journal of Asian Finance, Economics and Business
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    • 제7권12호
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    • pp.1027-1033
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    • 2020
  • This study intends to improve job performance during the Covid-19 pandemic at startup companies in Indonesia. In this study, the variables tested were job satisfaction, job innovation, Indonesian culture control, and job performance. Increasing job performance is deemed necessary in facing the economic crisis caused by the Covid-19 pandemic. Job innovation, job satisfaction, and culture control are deemed necessary in improving job performance. The population of this study are managers of start-up companies in Jakarta, Banten, and West Java. This research data obtained by distributing questionnaires to startup managers. This is a quantitative study with primary data. The sample technique used was purposive sampling. Structural Equation Model using Partial Least Square statistical software was used to analyze data. The results of this study indicate a change in the pattern of work performed by startup companies in running their business. Before the Covid-19 pandemic, employees worked in offices for seven hours, but after this pandemic, they change work patterns, moving them to work from home. Working from home requires companies to exercise better control and leadership patterns so that employees can work comfortably.

실험실내와 비닐하우스에서 근권 미생물에 의한 오이 생육증진의 검정 (In Vitro and Greenhouse Evaluation of Cucumber Growth Enhanced by Rhizosphere Microorganisms)

  • 배영석;장성식;박창석;김희규
    • 한국식물병리학회지
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    • 제11권4호
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    • pp.292-297
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    • 1995
  • We developed an in vitro assay method for evaluating plant growth promotion and providing an evidence that the growth promotion is rendered by growth enhancing factors. The amendment of culture filtrates of Trichoderma harzianum T95 and Gliocladium virens G872 and G872B in Murashige and Skoog (MS) agar medium enhanced the cotyledon growth of cucumber in terms of fresh weight and primary leaf area of cucumber cotyledon cuttings, of which the treatment of G. virens G872B was the most effective. The mycelial culture filtrate of G872B was more effective in the growth promotion than its conidial germling filtrate. The addition of 1% sucrose in MS mineral medium with 0.1% culture filtrates of the antagonists (T95 and G872B) was optimum for enhancing the effect of the filtrates on the growth of cotyledon cuttings in vitro. When cucumber seeds treated with G872B, Pseudomonas putida Pf3 or the G872B-Pf3 mixture were planted in a greenhouse, the rate of seed germination, biomass of shoot and root, and yield of cucumber fruits were increased, especially by G872B or the G872B-Pf3 mixture. Correspondingly, cucumber fruit yields in early to middle-cycles of harvest were significantly greater in the plots of G872B than the control and Pf3-treated plots, and the final yield was highest in the plots of the G872B-Pf3 mixture applications.

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Phenotype of Hepartocyte Spheroids in Synthetic Thermo-reversible Extracellular Matrix

  • Park, Keun--Hong;Park, Ju-Young;Bae, You-Han
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제6권4호
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    • pp.264-268
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    • 2001
  • Aggregates of specific cells are often regarded as better from in artificial organs and mammalian cell bioreactors in terms of cell-specific functionality. In this study, the morphology and liver specific functions of freshly harvested primary rat hepatocytes, which were cultivated as spheroids and entrapped in a synthetic thermo-reversible extracellular matrix, were examined and compared to a control (hepatocytes in single cell form). A copolymer of N-isopropylacrylamide(98 mole % in feed) and acrylic acid (poly (NiPAAm-co-AAC)), a thermo- reversible copolymer gel ma- trix, was used to entrap hepatocytes either in spheroids or single cells. During a 7-day culture pe-riod, the spheroids maintained higher viability and produced albumin and urea at a relatively con-stant rate, while, the single cell culture showed a slight increase in cell numbers and a reduction in albumin secretion Hepatocytes cultrured as spheroids present a potentially useful three-dimensional cell culture system for application in bioartificial liver device.

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Gas Chromatographic Profiling법을 이용한 Tridecanedioic Acid를 생산해내는 Candida tropicalis Mutant의 탐색연구 (Gas Chromatographic Profiling for the Screening of Candida tropicalis Mutant Producing Tridecanedioic Acid)

  • 김정한;이상준;박형국;김경례
    • 한국미생물·생명공학회지
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    • 제19권2호
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    • pp.135-139
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    • 1991
  • Tridecanedioic acid (DC-13), starting material of the valuable musk ethylene brassylate, was obtained from n-tridecane by the Candida tropicalis mutant. The mutants were first obtained from primary screening step using the selective medium and then solid phase extraction sampling method was used for the selective isolation of organic acids from the cultured media of mutants. The resulting acids were directly converted to volatile tert-butyldimethyl silyl delivatives, which were then analyzed by gas chromatography. The efficient GC profiling method was used for the rapid identification of the mutant producing DC-13 in large quantity, and for the optimization of the culture conditions of mutant. The optimal culture conditions were found as follows: pH 8.0, 30$^{\circ}C$, 250rpm, 48hour of culture and $(NH_4)_2HPO_4$ as nitrogen source.

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