• Clinical and Experimental Reproductive Medicine
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• 제51권1호
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• pp.42-47
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• 2024

Objective: This study evaluated the effects of temperature and storage time on the quality and DNA integrity of freeze-dried sperm from individuals with normozoospermia. Methods: Normal sperm samples from 15 men aged 24 to 40 years were studied. Each sample was divided into six groups: fresh, freezing (frozen in liquid nitrogen), freeze-dried then preserved at room temperature for 1 month (FD-1m-RT), freeze-dried then preserved at room temperature for 2 months (FD-2m-RT), freeze-dried then preserved at 4 ℃ for 1 month (FD-1m-4 ℃), and freeze-dried then preserved at 4 ℃ for 2 months (FD-2m-4 ℃). The morphology, progressive motility, vitality, and DNA integrity of the sperm were evaluated in all groups. Results: In all freeze-dried groups, sperm cells were immotile after rehydration. The freeze-dried groups also showed significantly less sperm vitality than the fresh and frozen groups. Significantly more morphological sperm abnormalities were found in the freeze-dried groups, but freeze-drying did not lead to a significantly higher DNA fragmentation index (DFI). The DFI was significantly higher in the FD-2m-RT group than in the other freeze-dried groups. Conclusion: The freeze-drying method preserved the integrity of sperm DNA. The temperature and duration of storage were also identified as factors that influenced the DFI. Accordingly, more research is needed on ways to improve sperm quality in the freeze-drying process.

• Journal of information and communication convergence engineering
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• 제8권3호
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• pp.339-342
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• 2010

An iris image pattern positioning with preserved edge detector, ring zone and clock zone, frequency distribution and overlay matching is presented in this paper. Edge detector is required to be powerful and detail. That is proposed by overlaying Canny with LOG (CLOG). The two reference patterns are made from allocating each gray level on the clock zone and ring zone respectively. The normalized target image is overlaid with the clock zone reference pattern and the ring zone pattern to extract overlapped number, and make a matched frequency distribution to look through a symptom and position of human organ and tissue. The iterating experiments result in the ring and clock zone positioning evaluation.

• 대한가정학회지
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• 제18권4호
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• pp.87-94
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• 1980

This study was carried out to make a comparison between iced and partial freezing of bastard, yellow tail, poragy, pomfret that were generally used for Sashimi and the results that measured k-value, VB-N, TMA-N were measured and the results are as follows : 1. Regardless of the kinds of fish, freshness is better preserved in partial freezing than in iced storage. 2. In Bastard, Yellow tail When iced its freshness estimation index its K-value rose above 20% after 4 days of storage. When stored partially frozen, its K-value reached 20% after 8 days. 3. Porgy when iced, its K-value reach 20% after 6 days of storage. But when stored partially frozen, its K-value could be prolonged until 9 days with same degree. Porge was preserved for the longest time among the four fishes. 4. Pomfret When iced during 4 days and stored partially frozen during 6 days, their K-value reached about 20% Pomfret was preserved for the shortest time among the four fishes. 5. According to the kinds of fish, the results that measured VB-N, TMA-N, total bacteria have some differences, but the method of partial freezing was superior to iced storage.

• 보존과학회지
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• 제35권6호
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• pp.588-599
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• 2019

발굴 조사 된 매장문화재는 학술적, 역사적 가치에 따라 문화재청장이 보존조치를 결정하지만, 이전·복원 후 관리, 보존, 활용은 체계적으로 이루어지고 있지 않고 있다. 또한 보존유적 및 보호시설의 손상으로 보존방안과 활용도에 대한 문제점이 발생되고 있다. 이에 충북 지역 보존유적에 대한 현황을 조사하여 보존방안 및 활용방안에 대하여 제시해 보고자 하였다. 충북에는 총 43개의 보존유적이 있었으며, 관리주체의 명확성 여부를 떠나 대부분 체계적인 관리는 이루어지고 있지 않았다. 잘못된 보존처리와 재료의 선정 등으로 인해 유구 및 보호시설이 훼손 되어지고 있는 상태로 확인되었으며, 보존유적의 훼손으로 전시, 교육, 체험 등 활용성에서도 문제점을 확인하였다. 이러한 문제점 해결에는 법적·제도적 장치의 개선, 예산·전문 인력 확보. 표준화 매뉴얼 개발 및 적용, 지속적 모니터링, 예방보존, 이전 지료 및 방법 연구, 목록화 작업, 전문 박물관 및 복합 테마 파크 조성 등 중·장기적인 계획이 세워져야 할 것이다. 또한 조사된 보존유적에 맞는 보존방안을 제시하고, 이를 기반으로 보존·관리가 이루어지면 보다 체계적·과학적으로 보존·관리되고 교육, 전시, 홍보 등 다양한 목적으로 활용될 수 있을 것으로 판단된다.

• 한국수정란이식학회지
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• 제33권1호
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• pp.23-30
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• 2018

The purpose of this study was to investigate the effects of the concentration of seminal plasma in aerobic and anaerobic conditions on the total motility(TM) and the progressive motility(PM) of spermatozoa in long term preservation of cooled equine semen. We also examine the pregnancy rates after artificial insemination using fresh, cooled or frozen semen, and different durations of cooled-preserved equine semen. In the aerobic state of cooled-preserved semen, As the increase of preserved duration to 24h, 48h, 72h, and 96h, TM tended to decrease in each of different concentrations of formalin-containing experimental group, TM tended to decrease regardless of the concentrations of SP. In different concentrations of SP, TM of without seminal plasma(SP W/O) group tended to be higher than that of SP 20%, SP 33% and SP 50%, especially TM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). PM was higher in the groups of SP W/O and SP 20% than in the groups of SP 33% and SP 50% from 24 h to 72 h in cooled-preservation, especially PM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). In the anaerobic condition of cooled-preserved semen, the results of TM and PM at different concentrations of SP were similar to the results in the aerobic condition although there was a difference in the ratio. The pregnancy rates of fresh-cooled, cooled-preserved and frozen semen were 66.3%, 60.7% and 34.5%, respectively, and the pregnancy rate of frozen semen was the lowest. We also found that it is possible to pregnancy after artificial insemination using 72 h cooled-preserved equine semen. There was similar of the pregnancy rates in the different month from April to August.

• Journal of Chest Surgery
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• 제24권7호
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• pp.712-718
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• 1991

The pectus excavatum or funnel shaped thorax is a relatively frequent malformation of the chest wall which composed of depressed sternum and costal cartilage. The so-called sternal turnover operation for pectus excavatum has been disappointing due to postoperative complication such as fistula formation with necrosis of bone and muscle. Thus, the need of preserved vascular pedicle to sternum was stressed in this type of operation, keeping the bilateral internal mammary vessels preserved. We have experienced two cases of modified sternal turnover in children and obtained satisfactory postoperative results.

• 한국지능시스템학회논문지
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• 제3권4호
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• pp.21-23
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• 1993

Under the maxmin compositional rule of inference which is used in applications while executing fuzzy algorithms, Pappis showed that the property of approximation is preserved. In this paper, we generalize a measure of proximity of fuzzy subsets on any set, without the restriction of finiteness. And it is shown that the same property of approximation if preserved under the supmin compositional rule of inference.

• 한국식품영양과학회지
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• 제32권4호
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• pp.526-530
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• 2003

각기 다른 염농도에서 염장한 오이의 이화학적 특성을 조사하였다. 염장시 첨가되는 염농도가 높을수록 수축현상이 심하였으며, 오이의 염농도는 염장 30일경에 30% 처리구를 제외한 모든 처리구에서 이론치와 유사한 농도를 나타내었다. pH의 경우 25%처리구는 염장 120일까지 감소 경향을 보인 반면 30%처리구는 60일이후부터 거의 변화가 없었다. 오이 표면의 녹색도는 염장 10일까지는 모든 처리구에서 녹색(-1.33~-2.87)이 유지되었으나 염장기간이 경과함에 따라 적색으로 변하였고, 이러한 현상은 염농도가 낮을수록 심하였다. 황색도는 염농도가 낮은 15% 첨가구가 염장 전기간에 걸쳐 가장 높은 황색도(6.15~12.52)를 보였다. Isopropyl alcohol 추출색소액 중 생오이의 색소추출액은 439, 473, 632 nm에서 피크를 나타낸 반면 염장오이는 염장초기 첨가된 염농도에 관계없이 생오이 색소추출액과는 다른 흡광도 변화를 나타내어 410, 505, 538, 607, 565 nm에서 새로운 피크를 형성하였다. 경도는 염농도에 관계없이 저장기간이 경과함에 따라 지속적으로 상승하였다.

• 한국강구조학회 논문집
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• 제12권3호통권46호
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• pp.259-268
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• 2000

마찰접합에 있어서 작용외력은 고력볼트로 체결된 접합부재간의 마찰면에 작용하는 마찰력에 의해 전달된다. 이 마찰력은 마찰면의 미끄럼계수와 고력볼트 조임력의 곱에 비례한다. 그러나 너트회전법에 의한 조임시 고력볼트 조임력은 많은 인자에 의해 영향을 받는다. 여유나사길이와 길이-직경비는 고력볼트 조임력을 지배하는 중요한 인자 중에 하나이다. 따라서 본 논문은 여유나사길이와 길이-직경비에 따른 고력볼트 세트(F10T, M16, M20, M22, M24)의 기계적 특성에 관한 실험을 통하여 고력볼트의 정확한 체결 방법을 제공하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권1호
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• pp.91-99
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• 1996

About 7.7 million tons of straw dry matter are being rotten during the monsoon. The objective of this trial was to develop a technique to preserve straw under wet condition. To simulate the moisture content of wet straw, a dry straw was deeped overnight in water. After draining the excess water, the wet straw(668 g moisture $kg^{-1}$) was divided into twenty fractions and preserved with 0, 30, 50 ro 70 g urea $kg^{-1}$ dry matter for either of 30, 60, 90, 120 or 180 days in sealed plastic container. Considering the colour, smell, fungal infestation and pH, the wert straw was preserved excellently up to 180 days when 50 or 70 g of urea per kg DM was used. Urea preservation increased the crude protein contents of straw by 3.6 to 6.4 times (174 to $364g{\cdot}kg^{-1}$) over that of the dry straw ($48g{\cdot}kg^{-1}$). Although the NDF content of straw was not effected by the level of urea or by the length of the preservation period, but the ADF content increased (p > 0.05) by 0.086 to $0.889g{\cdot}kg^{-1}$ straw DM for each g increase in the urea level. At 48 hours, the DM degradability of dry straw was $350g{\cdot}kg^{-1}$, which increased to $633g{\cdot}kg^{-1}$ when preserved with 50 g urea $kg^{-1}$ for 180 days. For the same straw, both the rate(0.0388 vs. 0.0136 fraction $h^{-1}$), the extent(717 vs. $631g{\cdot}kg^{-1}$) of straw degradation and the estimated ME(9.55 vs. $6.51MJ{\cdot}kg^{-1}$ straw DM) were higher in the preserved than the dry straw.