• Journal of Hospice and Palliative Care
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• 제15권3호
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• pp.147-154
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• 2012

목적: 본 연구의 목적은 의식 없는 말기환자 가족의 연명치료 중단 결정 경험을 이해하기 위함이다. 방법: 자료는 의 없는 말기환자의 연명치료 중단 결정을 한 8명의 가족으로부터 심층면담을 통해 수집하였다. Colaizzi의 현상학적 분석 방법이 자료 분석을 위해 사용되었다. 결과: 주제는 12개의 주제모음으로 분류되었고, 12개의 주제모음은 마침내 5개의 범주로 통합되었다. 5개의 범주는 환자상태로 인한 절망감, 의료진의 권고에 마음을 비움, 환자의 바램, 돌봄으로 인한 가족의 소진, 연명치료와 관련된 과거의 경험이었다. 결론: 다섯 개의 범주를 통하여 의식 없는 말기환자 가족의 연명치료 중단 결정 과정에서 발생할 수 있는 현상들을 이해함으로써 적절한 상담과 간호를 제공하여 보다 바람직한 호스피스 완화의료 중재가 필요하다고 본다.

• Journal of Periodontal and Implant Science
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• 제39권sup2호
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• pp.231-237
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• 2009

Purpose: Cytolethal distending toxin (CDT) is a family of heat-labile cytotoxins produced by several gram-negative mucosa-associated pathogens, including Aggregatibacter actinomycetemcomitans. CDT is well known to be capable of inducing growth arrest, morphological alterations, and eventually death in various cells. CDT belongs to a tripartite $AB_2$ toxin (CdtB: the enzymatic A subunit; CdtA and CdtC: the heterodimeric B subunit). Previous studies proposed that CdtA and CdtC together bind to a cell surface receptor and glycolipids act as a receptor for A. actinomycetemcomitans CDT (AaCDT). In this study, recombinant CdtA and CdtC proteins of AaCDT were co-expressed in a bacterial expression system and tested for their affinity for $GM_1$ ganglioside. Methods: The genes for CdtA and CdtC from A. actinomycetemcomitans Y4 were utilized to construct the expression vectors, pRSET-cdtA and pET28a-cdtC. Both CdtA and CdtC proteins were expressed in Escherichia coli BL21(DE3) and then purified using hexahistidine (His6) tag. The identity of purified protein was confirmed by anti-His6 antibody and monoclonal anti-CdtA antibody. Furthermore, the affinity of recombinant protein to $GM_1$ ganglioside was checked through ELISA. Results: Recombinant CdtA and CdtC proteins were expressed as soluble proteins and reacted to anti-His6 and monoclonal anti-CdtA antibodies. ELISA revealed that purified soluble CdtA-CdtC protein bound to $GM_1$ ganglioside, while CdtA alone did not. Conclusions: Co-expression of CdtA and CdtC proteins enhanced the solubility of the proteins in E. coli, leading to convenient preparation of active CdtA-CdtC, a critical material for the study of AaCDT pathogenesis.

• Bulletin of the Korean Chemical Society
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• 제31권9호
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• pp.2649-2655
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• 2010

$PKC{\delta}$-catalytic V5 Heptapeptide (FEQFLDI, FP7) interacts with heat shock protein 27 (HSP27) and inhibits HSP27-mediated resistance to cell death against various stimuli including radiation therapy. Here, we prepared radio-iodinated heptapeptide and further investigated its uptake properties in HSP27 expression cells. Peptide sequence of FP7 and a negative control peptide (WSLLEKR, QP7) was modified by substituting their C-terminus residue to tyrosine (FP6Y and QP6Y) to label radio-iodine. Iodinated peptides were confirmed by LC mass analysis with cold iodine reaction mixture. Accumulation of [$^{125}I$]iodo-FP6Y and [$^{125}I$]iodo-QP6Y in NCI-H1299 cell line, with higher level of HSP27, and NCI-H460 cell line, with lower level of HSP27, was measured by NaI(Tl) scintillation counter. The modification of substituting C-terminus residue of FP7 to tyrosine (FP6Y) did not affect its interaction with HSP27. Accumulation of [$^{125}I$]iodo-FP6Y in NCI-H1299 cells was 3 fold higher than in NCI-H460 cells. The novel radio-iodinated FP6Y would be used as a tracer for targeting HSP27 protein.

• 대한한방내과학회지
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• 제40권6호
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• pp.1136-1144
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• 2019

Objective: Physical inactivity contributes to mortality rates and is now the fourth most frequent cause of death worldwide. Red ginseng is a medicinal herb that is often used as an ergogenic aid. In this study, red ginseng was administered to rats to test whether it affected their ability to exercise. Methods: Forty-five rats were randomly distributed and divided into five groups: normal (N, n=5), control (C, n=10), the group to which only red ginseng was administered (H, n=10), the group to which only amino acid complex was administered (A, n=10), and the group to which both red ginseng and amino acid complex were administered (HA, n=10). Once a day for three weeks, 333.3 mg/kg body weight per day (b.w./day) of red ginseng and 750 mg/kg b.w./day of amino acid preparation were administered to rats. After three weeks, body weight, swimming time, and the weight of the anterior tibialis muscle of rat were measured. Blood was taken for analysis using the cardiac puncture method. Results: The swimming time of group H (921.3±199.26 sec) showed significant improvement compared to that of group C (798.48±156.37 sec) (p<0.05). Conclusion: Red ginseng has improved swimming time in rat and can be used as an effective ergogenic aid.

• 생약학회지
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• 제30권3호
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• pp.255-260
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• 1999

In the previous report, E-kong-san, which is usually used for recovering health in traditional medicine, has been shown to decrease cisplatin induced nephrotoxicity in vivo and in vitro. The significant reduction of E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of triggering antioxidation. In monkey kidney Vero cells, E-kong-san at $5{\sim}10\;mg/ml$ was able to attenuate 2mM cisplatin-stimulated cell death by 46.8% and 31.8%, respectively. E-kong-san showed strong free radical scavengering activities on 1,1-diphenyl-2-picrylhydrazil (DPPH) radical and xanthine/xanthine oxidase (XOD) generated superoxide anion radical $(O_2^{-.})$. We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. Moreover, E-kong-san exhibited significant inhibition on both ascorbic $acid/Fe^{2+}$ and $ADP/NADPH/Fe^{3+}$ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that E-kong-san protects the cisplatin induced cytotoxicity by its antioxidative mechanism.

• 대한수의사회지
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• 제23권9호
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• pp.603-610
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• 1987

The pathological and microbiological studies were carried out to investigate an acute, febrile, highly fetal, infectious disease of rabbits that had occurred in the Winter and in the Spring and that had begun to be reported in Korea from November, 1985. The clinical signs of this disease were characterized by high fever, lethargy, piercing shriek, convulsion, and sudden death with epistaxis, but often they were not observed. The predominant pathogical findings were severe congestion and hamorrhage in trachea, dark brown discoloration of liver by diffuse necrosis or acute viral hepatitis, and hamorrhagic damages of lung, heart, spleen, kidney, etc. The etiological agent was a small round virus, in 25-35nm in diameter and without envelope, thus looking like a picorna virus. This disease resembled what was called the 'Viral Hamorrhagic Pneumonia in Rabbits'(tentative name) that had been reported for the first time in China in 1984. It will be desirable that the disease should be renamed as the 'Viral Hemorrhagic Fever in Rabbits', the 'Acute Viral Hepatitis in Rabbits', etc. because of its charateristics and the basis of pathological findings. An inactivated vaccine is now in the process of preparation for the prophylaxis of this viral disease.

• 대한가정학회지
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• 제38권3호
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• pp.85-97
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• 2000

The goal of this study is mainly to improve the quality of life for the elderly by understanding the actual condition of the home management which includes their family relationship, family finance, and household work carried out in their domestic lives and diagnosing problems possibly restated from the condition. The result includes the following. The characteristics of rural elderly living alone: They have lived in rural area since their marriage which is over 40 years long in average, The tend to be satisfied with living apart themselves. The majority of the families are the first son in their family. In home management regard: First, in family relationship, the way the elderly in their nuclear family state communicate with their spouse is limited, and they hardly speaks together. So it is encouraged for couples to forge their own specific relationship and to plan for their odd age in their younger age. Also, they tend to be satisfied with living separated from their children, but at the same time, the closer they live in distance from their children, the more often they meet their children as well as they talk on the phone. They especially depend on their first son for offerings for ancestors and matters to occur after their death, while they get emotional support from daughters. Second, in family finance, their income varies in range from 200,000 to 3,000,000 won. In the majority f the famines, the wives are in charge of finance. The cost of living is mainly spent on flood, clothing, and housing, or the cost of offerings for ancestors if they are the head family. Third, Meanwhile, the housewives under 70 years old are positive about household work and tend to consider it pleasant for their children and their husband. Generally, the older they are, the more they dislike household labor. For example, food preparation is especially considered a major problem. Community dinning rooms, food delivery service, volunteers, and home helper and neighborhood could be help.

• Advances in Traditional Medicine
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• 제9권2호
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• pp.106-114
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• 2009

Hepatocellular carcinoma is the world's most common primary malignant tumor of the liver. In-Jin-ho-Tang (IJHT) has been used as a traditional Chinese herbal medicine since ancient times, and today it is widely used as a medication for jaundice associated with inflammation of the liver. In-Jin-Ho-Tang is a drug preparation consisting of three herbs: Artemisiae Capillaris Herba (Artemisia capillaries $T_{HUNS}$, Injinho in Korean), Gardeniae Fructus (Gardenia jasminodes $E_{LLIS}$, Chija in Korean) and Rhei radix et rhizoma (Rheum palmatum L., Daehwang in Korean). This study investigated whether or not methanol extract of IJHT could induce HepG2 cancer cell death. Cytotoxic activity of IJHT on HepG2 cells was measured using an XTT assay, with an $IC_{50}$ value of $700{\mu}g/ml$ at 24 h Apoptosis induction by IJHT in HepG2 cells was verified by the cleavage of poly ADP-ribose polymerase, and a decrease in procaspase-3, -8, -9. Treatment of IJHT resulted in the release of cytochrome c into cytosol, loss of mitochondrial membrane potential (${\Delta}{\Psi}_m$), decrease in anti-apoptotic Bcl-2, and an increase in pro-apoptotic Bax expression. Thus, IJHT induced apoptosis in HepG2 cells via activation of caspase and mitochondria pathway. These results indicate that IJHT has potential as an anti-cancer agent.

• 조명전기설비학회논문지
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• 제23권6호
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• pp.52-58
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• 2009

최근 건축구조물의 복잡화, 다양화로 인하여 화재발생시 유독가스의 심각성과 공간의 특수성으로 대형 참사가 발생될 위험이 더욱 커짐에 따라 인명피해와 직접적으로 연결되는 피난탈출에 있어서 조속한 대책 마련이 시급한 실정이다. 기존 방식의 고정식 단방향을 표시하는 피난유도등은 효율적인 인명 대피 및 구조가 이루어지지 못하기 때문에 화재 발생 시 빠른 피난이 가능하도록 화재감지기와 연동하여 위험에 처한 피난자들을 탈출시키고 화재의 발생위치를 정확히 파악하여 초기 진압할 수 있는 시스템 구축이 절실히 요구된다. 이러한 점에 착안하여 ID를 가지는 화재 감지기 및 수신반과 연동하여 화재가 발생하면 화재가 발생된 방향의 반대 방향으로 모든 유도등의 방향을 제어하여 안전한 비상출입구로 사람들을 유도시키고 화염 및 연기의 방향과 속도를 계산하여 포기진압 및 최적 대피로로 방향을 표시하는 인공지능형 방향성 유도등 개발에 본 연구의 목적이 있다.

• 한국콘텐츠학회논문지
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• 제21권4호
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• pp.555-564
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• 2021

본 연구는 기존 노인돌봄서비스의 사회적가치지향성과 서비스 이용 노인의 안녕감을 평가하고, 돌봄 욕구에 대한 수요를 확인하여 우리나라 노인돌봄 정책의 발전 방향을 모색하고자 실시되었다. 2019년 10월 기준 3개월 이상 노인돌봄기본서비스를 이용한 노인 1,501명을 편의표집하였으며, 빈도분석 및 기술통계분석, t-검정, 분산분석 등을 실시하였다. 분석 결과 돌봄 서비스의 사회적가치지향성은 평균 90.93점이며, 돌봄 서비스 이용 노인의 안녕감은 71.29점으로 나타났다. 분산분석 및 사후분석 결과 사회적가치지향성을 보다 높다고 인식한 집단에서 노인들의 안녕감이 통계적으로 유의미하게 높았다. 한편 돌봄 서비스 중단 시 우려되는 점에 대해 "외로움", "고독사", "안전" 등의 키워드가 도출되었으며, 향후 필요한 서비스로 '일상생활 지원 서비스'에 대한 욕구가 가장 높았다. 본 연구결과를 토대로 노인돌봄서비스의 발전 방향 모색 및 노인돌봄 체계 구축에 기초자료로 활용될 수 있을 것이다.