• 한국가정과교육학회지
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• 제23권4호
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• pp.1-17
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• 2011

본 연구는 청소년의 전통 의생활 문화인식을 증진시키기 위하여 의생활 단원 내용을 중심으로 교수 학습 과정안을 개발하고 적용하였다. 7차 교육과정 고등학교 1학년 교과서 12종과 7차 개정교육과정을 분석하고 이를 바탕으로 '가정생활문화 변화'단원을 중심으로 '전통문화', '한복의 변천사', '한복의 색채', '한복의 문양과 소재', '한복의 세계화' 5개의 학습 주제를 선정하였다. 선정한 주제에 대해, 5차시의 교수 학습 과정안 및 48종의 수업자료(학생자료 22개, 수업자료 22개, 교사 자료 4개)를 개발하고 수업에 적용하였다. 청소년들의 전통의생활 문화에 대한 인식도는 사전검사 평균에 비해 사후 검사 평균이 높게 나타난 것으로 보아 본 연구의 교수 학습 과정안이 전통 의생활 전통문화에 대한 올바른 인식, 외래문화에 대한 수용, 전통 문화에 대한 긍정적 자세 등 전통 문화 인식 고취에 효과적이었다고 할 수 있다. 전통의생활 교육에 대한 인식 또한 유의미하게 향상되어 전통 문화교육의 중요성을 알 수 있었다. 이에 본 연구자는 의생활 문화 교육과 관련하여 더 다양한 전통문화 관련 주제가 개발되어야 하며 더 강화되어야 한다고 제언하는 바이다. 뿐 아니라 식생활, 주생활 분야에도 전통문화 교수 학습 과정안이 개발되고 적용되는 등 다양한 전통문화 계승 교육 연구가 이루어질 필요가 있다.

• 한국축산식품학회지
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• 제40권4호
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• pp.512-526
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• 2020

Synthetic nitrite is considered an undesirable preservative for meat products; thus, controlling synthetic nitrite concentrations is important from the standpoint of food safety. We investigated 1,000 species of microorganisms from various kimchi preparations for their potential use as a starter culture for the production of nitrites. We used 16S rRNA gene sequence analysis to select a starter culture with excellent nitrite and nitric oxide productivity, which we subsequently identified as Staphylococcus hominis subspecies hominis WiKim0113. That starter culture was grown in NaCl (up to 9%; w/v) at 10℃-40℃; its optimum growth was observed at 30℃ at pH 4.0-10.0. It exhibited nonproteolytic activity and antibacterial activity against Clostridium perfringens, a bacterium that causes food poisoning symptoms. Analysis of Staphylococcus hominis subspecies hominis WiKim0113 with an API ZYM system did not reveal the presence of β-glucuronidase, and tests of the starter culture on 5% (v/v) sheep blood agar showed no hemolytic activity. Our results demonstrated the remarkable stability of coagulase-negative Staphylococcus hominis subspecies hominis WiKim0113, especially in strain negative for staphylococcal enterotoxins and sensitive to clinically relevant antibiotics. Moreover, Staphylococcus hominis subspecies hominis WiKim0113 exhibited a 45.5% conversion rate of nitrate to nitrite, with nitrate levels reduced to 25% after 36 h of culturing in the minimal medium supplemented with nitrate (200 ppm). The results clearly demonstrated the safety and utility of Staphylococcus hominis subspecies hominis WiKim0113, and therefore its suitability as a starter culture.

• 대한가정학회지
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• 제41권11호
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• pp.135-150
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• 2003

This research was to verity the effects of ICT teaching method in high school home economics. The research questions for this study were as follows: 1. Is the ICT teaching method effective in increasing students' interest in home economics and consciousness on traditional culture in teaming the unit of ‘Korean traditional culture of home life’？ 2. Is the ICT teaching method effective in improving students' achievement test and performance assessment in teaming the unit of ‘Korean traditional culture of home life’？ This study is carried out with following procedure: developing the homepage and lesson plans for ICT teaching, constructing questionnaire, and testing its reliability, pre-testing, treatment for 8 weeks, and post-testing. Two groups were arranged for 8 weeks experiment from May 7,2001 through June 30,2001. Sixteen hours of ICT teaching and 8 hours of traditional lecture for experiment group whereas 8 hours of ICT teaching and 16 hours of traditional lecture for comparison group were input. SAS program was used for statistical analysis, and independent sample t-test and matched pairs t-test were peformed for answering research questions. The results of this study were as following: 1. Sixteen hours of ICT teaching and 8 hours of traditional lecture for experiment group was effective in increasing consciousness on traditional culture and performance assessment. 2. Eight hours of ICT teaching and 16 hours of traditional lecture for comparison group was not effective in learning home economics. This means that at least 2 hours of ICT teaching is demanding for effective learning. This study concluded that ICT teaching will be effectively applied in high school home economic with well developed ICT teaching materials. And home economics educators should continuously put effort in developing curricular, teaching materials, and teaching methods to raise students' interest in home economics.

• 생물환경조절학회지
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• 제2권1호
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• pp.37-45
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• 1993

국내 원예시설이 고정화되면서 각종 연작 장해가 나타남에 따라 객토와 토양소독의 필요성이 커지고 있으나 객토와 토양소독에 소요되는 비용 증가가 큰 문제로 대두되고 있다. 이에 따른 간이한 양액재배 시스템으로서 농가에서도 투자비용과 운용비용이 저렴하면서 환경오염의 염려가 없거나 적은 각종 인공배지를 활용한 토마토 자루재배(bag culture) 실험결과는 다음과 같다. 1. 단용배지를 이용한 토마토 자루배재에서 질석, 훈탄, 모래, 페놀수지 (PRE), 입상면, perlite 등에서 생육, 수량 및 품질면에서 이용가능성이 높고 이들 배지의 단점을 보완한다면 암면 대체도 가능할 것으로 보인다. 2. 혼합배지를 이용한 토마토 자루재배에서 perlite와 입상면 혼합처리에서는 perlite 20-40％수준과 입상면 80-60％수준에서, peatmoss와 질석 혼합처리에서는 peatmoss 40-60％와, 질석 60-40％ 혼합비율에서, peatmoss+질석 +입 상면 혼합처리에서는 peatmoss를 50-60％로 하고 질석과 입상면을 각각 25-20％로 혼합한 경우에서 각각 높은 수량과 품질을 보여주었다.

• Journal of Microbiology
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• 제45권6호
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• pp.515-520
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• 2007

Vaginal lactic acid-producing bacteria of 80 pre-menopausal women were studied by isolation on Blood and DeMan-Rogosa-Sharpe agar, PCR with group-specific primers for Lactobacillus-denaturing gradient gel electrophoresis (DGGE), and PCR with specific primers for V3 region in 16S rRNA-temporal temperature gel electrophoresis (TTGE). Conventional isolation method on media detected only one lactobacillus (Lactobacillus brevis) while TTGE detected only Lactobacillus sp. DGGE detected seven Lactobacillus species; L. coleohominis, L. crispatus, L. iners, L. reuteri, L. rhamnosus, L. vaginalis, and Leuconostoc lactis. L. acidophilus and L. gasseri, which are prevalent in Western women, were not detected in Korean women. Furthermore, L. rhamnosus, Leuc. lactis, L. coleohominis, and Weissella cibaria, which were not previously reported in the vaginal microbiota of Korean women, were detected. The five most prevalent LABs in vaginal microbiota in Korean women were L. iners, Enterococcus faecalis, L. crispatus, Leuc. lactis, and W. cibaria.

• Journal of Microbiology and Biotechnology
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• 제3권2호
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• pp.65-72
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• 1993

A heterologous gene expression and secretion system using a methylotrophic yeast, Hansenula polymorpha was developed for the production of anticoagulant hirudin. Hirudin gene was expressed under the control of a strong and inducible methanol oxidase (MOX or AOX) promoter. The mating factor a pre-pro leader sequence of Saccharomyces cerevisiae was employed for hirudin to be secreted into the extracellular medium. Hirudin expression cassette was introduced into three strains of H. polymorpha, A16, HPBl and DLl which have different genetic backgrounds. This expression cassette was stably integrated into the host chromosomal DNA. Biologically active and mature hirudin was efficiently expressed and secreted into the extracellular medium. About 19 mg/L of hirudin was found in the culture supernatant in the case of a two-copy integrant of the strain HPBl under suboptimal culture conditions.

• 복식문화연구
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• 제1권2호
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• pp.145-157
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• 1993

This study aimes to investigate the garment ease of pe-school children's clothing in accordance with arm movement. The experiment was done with 4 per-school children fro mage 3 to age 5, and 3 types of experimental clothes were made ; sleeveless, half sleeve, and long sleeve. The waist pattern and the sleeve pattern of each experimental clothes has no garment ease. And experimental clothes were examined to obtain the necessary ease of armcye line and waist lien by cross-cut method. The results of investigation can be summarized as follows ; 1. The resulting movement ranges of experimental clothes with no garment ease were 131.5°(sleeveless), 71.75°(half sleeve), and 62.25°(long sleeve). 2. The size order of cross-cut opening of waist lien movement were (side>front>back), and (half sleeve>long sleeve>sleeveless). 3. When the base point of measurement was set to armpit point by arm the latitudinal length of cross-cut opening of armcye line by arm movement was (anterior armpit part > posterior armpit part), and longitudinal length of cross-cut opening was (upper part>lower part).

• 한국식생활문화학회지
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• 제17권4호
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• pp.473-481
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• 2002

Pyebaek of Jeonju area based on the method prepared by late old madame Park, Bokja can be characterized as follows: First, vegetables which could be obtained locally constituted the main body of materials of Jeonju Pyebaek and animal foods used in Pyebaek were dried fishe for the most of times. In the pre-refrigerator era, the main reason behind the heavy uses of dried fish such as dried abalone or octopus instead of animal foods can be attributed to the easy availability of dried fish. In recent years, foods made of animal foods has been indispensible items. Second for the patterns and decorations of Pyebaek natural figures such as birds, flowers, butterflies, loaves and half moon were imitated in making of octopus cuttings, squid cuttings, Yakkwa, Hwajeon, Dried persimmons, Jeonbokssam, etc. Beef jerky, Yeotgangjeong were decorated beautifully with pine nuts, shredded dried dates and raisins. Many colors were used in various items of Jeonju Pyebaek such as Hwajeon, Songpyun, Saji. Finally, flavors of Jeonju Pyebaek are sweet and tasty of sesame oil.

• Journal of Microbiology and Biotechnology
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• 제1권4호
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• pp.266-273
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• 1991

Hirudin, a 65-amino acid protein isolated from the salivary gland of the bloodsucking leech, Hirudo medicinalis, is a potent thrombin-specific inhibitor and blocks the thrombin-mediated conversion of fibrinogen to fibrin in clot formation. We have studied the gene expression and secretion of hirudin in yeast. Saccharomyces cerevisiae. A gene coding for hirudin was synthesized based on the amino acid sequence and cloned into a yeast expression vector $YEG{\alpha}-1$ containing the ${\alpha}-mating$ factor pre-pro leader sequence and galactose-inducible promoter, GALl0. Recombinant S. cerevisiae was found to secrete biologically active hirudin into the extracellular medium. The secreted recombinant hirudin was recovered from the culture medium and purified with ultrafiltration and reverse phase high performance liquid chromatography. Approximately 1 mg of hirudin per liter was produced under suboptimal culture conditions and brought to about 90% purity in two steps of purification.

• International Journal of Advanced Culture Technology
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• 제6권3호
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• pp.142-150
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• 2018

This paper describes the algorithm that lowers the dimension, maintains the object recognition and significantly reduces the eigenspace configuration time by combining the edge orientation histogram and principle component analysis. By using the detected object region as a recognition input image, in this paper the object recognition method combined with principle component analysis and the multi-layer network which is one of the intelligent classification was suggested and its performance was evaluated. As a pre-processing algorithm of input object image, this method computes the eigenspace through principle component analysis and expresses the training images with it as a fundamental vector. Each image takes the set of weights for the fundamental vector as a feature vector and it reduces the dimension of image at the same time, and then the object recognition is performed by inputting the multi-layer neural network.