• Title/Summary/Keyword: Pre-culture

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Fraud Risk Management Model: A Content Analysis Approach

  • MADAH MARZUKI, Marziana;NIK ABDUL MAJID, Wan Zurina;AZIS, Nur Kamaliah;ROSMAN, Romzie;HAJI ABDULATIFF, Nik Kamaruzaman
    • The Journal of Asian Finance, Economics and Business
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    • 제7권10호
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    • pp.717-728
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    • 2020
  • The objective of this study is to explore the whole process of fraud risk management strategies that should be implemented by the organizations. Secondly, this study discusses the governance issues that arise at each stage of the process. For the purpose of this study, a content analysis of previous literatures is used as a technique for gathering data. This process usually involves codifying qualitative and quantitative information into pre-defined categories in order to derive patterns in the presentation and reporting of information. Based on our content analysis, we found that the fraud risk management process should be made of at least five stages which are inculcating the culture of managing risks in an organization, identifying the risks, evaluating the risks, determining preventive actions and implementing and reviewing stages. Our extended analysis of the fraud risk management process finds that a lot of governance issues arise in the fraud risk management process that should be solved by regulators and companies in order to ensure that fraud risk management process is embedded as corporate culture, not merely as a process. Among them are how to create the risk culture in an organization and whether auditors and risk management committees identify risks from each available source.

Pre-immigration Screening for Tuberculosis in South Korea: A Comparison of Smear- and Culture-Based Protocols

  • Lee, Sangyoon;Ryu, Ji Young;Kim, Dae-Hwan
    • Tuberculosis and Respiratory Diseases
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    • 제82권2호
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    • pp.151-157
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    • 2019
  • Background: Tuberculosis (TB) is the most important disease screened for upon patient history review during preimmigration medical examinations as performed in South Korea in prospective immigrants to certain Western countries. In 2007, the U.S. Centers for Disease Control and Prevention (CDC) changed the TB screening protocol from a smear-based test to the complete Culture and Directly Observed Therapy Tuberculosis Technical Instructions (CDOT TB TI) for reducing the incidence of TB in foreign-born immigrants. Methods: This study evaluated the effect of the revised (as compared with the old) protocol in South Korea. Results: Of the 40,558 visa applicants, 365 exhibited chest radiographic results suggestive of active or inactive TB, and 351 underwent sputum tests (acid-fast bacilli smear and Mycobacterium tuberculosis culture). To this end, using the CDOT TB TI, 36 subjects (88.8 per $10^5$ of the population) were found to have TB, compared with only seven using the older U.S. CDC technical instruction (TI) (p<0.001). In addition, there were six drug-resistant cases which were identified (16.7 per $10^5$ of the population), two of whom had multidrug-resistance (5.6 per $10^5$ of the population). Conclusion: The culture-based 2007 TI identified a great deal of TB cases current to the individuals tested, as compared to older U.S. CDC TI.

Mouse Granulocyte-marcrophage Colony-stimulating Factor Enhances Viability of Porcine Embryos in Defined Culture Conditions

  • S. H Jun;X. S Cui;Kim, N. H
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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    • pp.71-71
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    • 2003
  • Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multifunctional cytokine that has been implicated in the regulation of pre-implantation embryo development across several species. The aim of this study was to determine the effects of mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF) on development of porcine parthenotes and nuclear transferred embryos, and on their expression of implantation-related genes. In the presence of bovine serum albumin, mGM-CSF did not increase the percentage of oocytes that developed to the blastocyst stage and at day 7 did not increase oocyte cell number. Addition of 10 mM GM-CSF to protein-free culture medium significantly increased the compaction and blastocoel formation of 1- to 2-cell parthenotes and cloned embryos developing in vitro. However, cell number was not increased when they were cultured in the presence of GM-CSF. Semi-quantitative reverse transcripts polymerase chain reaction (RT-PCR) revealed that mGM-CSF enhances mRNA expression of the leukemia inhibitory factor receptor, but does not influence interleukin-6 or sodium/glucose co-transporter protein gene expression in blastocyst stage parthenotes. These results suggest that mGM-CSF may enhance viability of porcine embryos developing in vitro in a defined culture medium.

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곡류 및 두류를 이용한 젖산균 전배양용 식용 배지의 제조 (Edible Culture Media from Cereals and Soybeans for Pre-cultivation of Lactic Acid Bacteria)

  • 박소림;박선현;장지은;양혜정;문성원;이명기
    • 한국식품영양과학회지
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    • 제42권6호
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    • pp.991-995
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    • 2013
  • 본 연구는 곡류 및 두류를 이용하여 식용 가능한 젖산균 모배양용 식용배지를 개발하고자 실시하였다. 식용 배지의 제조를 위하여 곡류는 발아현미, 찰현미, 발아찰현미를 사용하였고, 두류는 대두콩(대두콩, 껍질을 제거한 대두콩, 발아 대두콩, 껍질을 제거한 발아 대두콩)과 검은 대두콩(검은 대두콩, 껍질을 제거한 검은 대두콩, 발아 검은 대두콩, 껍질을 제거한 발아 검은 대두콩)으로 나누어서 젖산균 증식배지를 제조하였다. 실험에 사용된 젖산균은 Lactobacillus(Lb.) farciminis, Lb. homohiochii, Lb. pentosus, Lb. plantarum, Leuconostoc(Leu.) paramesenteroides, Leu. citreum 및 Leu. lactis 총 7종이다. 곡류를 이용한 식용배지에서 배양한 경우, 7개 종균의 평균 균수는 7.6~8.0 log CFU/mL의 균수를 나타내었다. 대두콩으로 제조한 식용배지는 껍질을 제거한 발아 대두콩 배지에서 대부분의 균이 높은 증식량을 나타내었다. 특히 Lb. plantarum은 10.08 log CFU/mL로 가장 높은 균수를 보였다. 검은 대두콩의 경우는 껍질을 제거한 발아 검은 콩배지에서 증식량이 많았다. Lb. homohiochii는 껍질제거 발아 검은 콩배지에서 9.90 log CFU/mL로 가장 많은 증식량을 보였다. 모든 결과에서 곡류와 두류를 이용한 식용배지를 이용하여 배양할 때 젖산균 증균량이 우수함을 알 수 있었고, 특히 가장 증식량이 우수한 '껍질을 제거한 발아 검은 대두콩' 식용 배지를 향후 젖산균 모배양에 이용하는 것을 권장한다.

Cytosine Arabinoside 전처리가 척수후근절 외식편 배양에 미치는 영향 (Effect of Cytosine Arabinoside Pre-treatment in Dorsal Root Ganglion Explant Culture)

  • 정호영;한성민;박종웅;윤인찬
    • 대한의용생체공학회:의공학회지
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    • 제36권6호
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    • pp.296-301
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    • 2015
  • Explant culture condition of dorsal root ganglion have been used to investigate the pathophysiology of peripheral nerve injury, while applying for the various clinical symptom such as trauma, pressure, and stretch. However, explant culture is usually contaminated by mitotic cells, which may observed as a newly divided cells including fibroblast or glia. The mitotic cells could be able to interrupt and change the cell signaling that make it difficult to avoid detrimental effects during the experiments. To eliminate mitotic cells, anti-mitotic reagents like mixture of uridine and 5-fluorodeoxyuridine or cytosine arabinoside were added to the cultures on the following day, but there is no research that investigate viability of anti-mitotic reagent in dorsal root ganglion explant culture. In this study, we investigate inhibition effect of cytosine arabinoside to mitotic cells in dorsal root ganglion explant culture. Also we visualized and analyzed anti-mitotic effect and toxicity of cytosine arabinoside in various concentration condition. This dorsal root ganglion explant culture condition can be applied to research that effect and mechanism of various stimulation and chemical application which affect peripheral nerve regeneration.

예비교사를 위한 수학답사활동 수행 결과 분석 (The Analysis on the Results of Mathematics Field Trips for Pre-service Mathematics Teacher)

  • 서보억
    • 한국수학교육학회지시리즈E:수학교육논문집
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    • 제30권2호
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    • pp.139-159
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    • 2016
  • 본 연구는 수학교육의 현장을 교실이 아닌 교실 밖으로 확장할 수 있다는 것을 전제로 진행되었다. 최근 수학교육은 다양한 현장체험활동, 실생활을 기반으로 한 수학교육 등의 중요성이 증가되고 있고, 이로 인해 예비교사교육에서도 이에 대한 고려가 필요한 실정이다. 이에 본 연구에서 예비수학교사를 대상으로 수학답사활동을 진행하고, 그 구체적인 적용 결과를 분석하였다. 우리 고유의 역사적 문화재 속에서 실시한 예비교사의 수학답사활동 전후에 대한 인식의 변화에 대한 양적 분석과 더불어, 수학답사활동의 목적인 인지적 효과, 정의적 효과, 문화-수학적 효과, 수학수업 개선 효과 및 수업목표지식, 수업내용지식 측면에서 질적 분석을 실시하였다. 연구결과 수학답사활동은 예비수학교사에게 유의미한 효과가 있는 것으로 나타났고, 새로운 교수학습 방법으로 정착하기 위해 지속적인 연구가 기대된다.

예비사서교사의 교육실습에 대한 현상학적 연구 (A Phenomenological Study of the Pre-service Teacher Librarian During Student Teaching Practice)

  • 권선영;박장순
    • 한국도서관정보학회지
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    • 제45권3호
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    • pp.321-348
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    • 2014
  • 본 연구에서는 교육실습과정을 체험하면서 느꼈던 개인의 경험을 심층적으로 살펴보고 실습과정 전반과 실습과정을 둘러싼 모든 환경을 총체적으로 이해해보고자 하였다. 이를 위해 가장 최근 교육실습을 경험한 연구 참여자를 선정하여 심층 면담을 진행하였으며 Giorgi의 현상학적 분석방법으로 자료를 분석하였다. 분석 결과 예비사서 교사의 교육실습에 대한 경험 구성요소는 '성장과정을 통한 도서관과 사서교사에 대한 인식의 형성', '진로설계에 대한 구체화와 실행전략', '실습경험을 통한 자기성장의 욕구', '조직문화 이해와 커뮤니케이션 관계 인지', '도서관과 사서교사에 대한 현실 직시'로 요약되었다. 이러한 연구 참여자들의 경험 요소는 시간적 흐름에 의한 맥락에서 이해될 수 있는 현상인 것을 알 수 있었다. 본 연구의 결과는 예비사서교사를 위한 교육실습에 대한 전반적인 검토와 환경을 개선하는데 활용될 수 있을 것을 기대한다.

Preconditioning for Cryopreservation of in vitro Grown Bulblets of Lily using Droplet-Vitrification

  • Song, Jae-young;Lee, Young-yi;Yi, Jung-yoon;Lee, Jung-ro;Yoon, Mun-sup
    • 한국자원식물학회지
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    • 제33권6호
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    • pp.689-695
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    • 2020
  • This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'Milky way'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

Towards a Student-centred Approach to Translation Teaching

  • Almanna, Ali;Lazim, Hashim
    • 비교문화연구
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    • 제36권
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    • pp.241-270
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    • 2014
  • The aim of this article is to review the traditional methodologies of teaching translation that concentrate on text-typologies and, as an alternative, to propose an eclectic multi-componential approach that involves a set of interdisciplinary skills with a view to improving the trainee translators' competences and skills. To this end, three approaches, namely a minimalist approach, a pre-transferring adjustment approach and a revision vs. editing approach are proposed to shift the focus of attention from teacher-centred approaches towards student-centred approaches. It has been shown that translator training programmes need to focus on improving the trainee translators' competences and skills, such as training them how to produce and select among the different versions they produce by themselves with justified confidence as quickly as they can (minimalist approach), adjust the original text semantically, syntactically and/or textually in a way that the source text supplely accommodates itself in the linguistic system of the target language (pre-transferring adjustment), and revise and edit others' translations. As the validity of the approach proposed relies partially on instructors' competences and skills in teaching translation, universities, particularly in the Arab world, need to invest in recruiting expert practitioners instead of depending mainly on bilingual teachers to teach translation.

Preconditioning for Cryopreservation of in vitro Grown Bulblets of Lily using Droplet-Vitrification

  • Jae-young Song;Jinjoo Bae;Young-yi Lee;Jung-yoon Yi;Jung-ro Lee;Mun-sup Yoon
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2021년도 춘계학술대회
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    • pp.35-35
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    • 2021
  • This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'MilkyWay'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 ㎕ PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.