• 제목/요약/키워드: Pre-culture

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The Effects of Mobile Accommodation App Quality Perception on Continual Use Intention through Expectation Confirmation and Satisfaction

  • Arum Park;Sin-Bok Lee
    • International Journal of Advanced Culture Technology
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    • 제11권2호
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    • pp.345-357
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    • 2023
  • This research investigates the relationship between the information quality, service quality, and system quality of lodging apps and the users' expectations, level of satisfaction, and intent to continue using them. For this objective, 418 respondents participated in a survey. To evaluate the hypotheses, the collected data were examined using SPSS 22.0 and AMOS 22.0 statistical software. This study constructed a model using information quality, service quality, system quality, expectancy, satisfaction, and intention to continue using the pre- and post-use relationship of users of accommodation applications. The results of testing the hypotheses indicated that system quality had no significant effect on expectancy, system quality and service quality had no significant effect on satisfaction, and all other hypotheses had significant effects. The conclusion of this research is that the app's system quality, including access speed, access barriers, and privacy, does not satisfy pre- and post-use expectations. In addition, the system quality and service quality of the application have little effect on the app's satisfaction. The information quality of the application has a considerable impact on expectation confirmation and satisfaction, expectation confirmation has an impact on satisfaction, and expectation confirmation and satisfaction have an impact on intention to continue using.

서울.경기지역 학교급식에서 과일류의 전처리시 세척 및 소독 방법에 대한 조사 (A Survey of Washing and Sanitizing Methods for the Pre-preparation of Fruits at a School Foodservice in the Seoul and Kyunggi Area)

  • 박종숙;박신인
    • 한국식생활문화학회지
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    • 제24권1호
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    • pp.39-50
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    • 2009
  • The principal objective of this study was to evaluate the sanitary management status of chlorine sterilization methods used for raw fruits in a school foodservice, and to suggest basic data for sanitary improvements in the quality of raw fruits. A questionnaire form predicated on HACCP standards was developed and utilized for self-reported evaluations of dietitians regarding their sanitary management practices. The subjects consisted of 257 dietitians that were employed in school (elementary middle high school) foodservices. The collected data were analyzed with the SAS package. According to the results of this study, it was deemed necessary that optimized sterilization and washing methods for good microbiological safety and quality of strawberries and bananas in school foodservice should be determined. Some strategies for future improvement were also suggested. They included the following: (1) Improvement of policy for assuring the quality of raw fruits by designing some sanitation standards and specifications for raw fruits; (2) Strengthening the research and accumulation of background data regarding methods for the sanitation of raw fruits; (3) Enforced improvement of personal hygiene for dietitians and employees; (4) Use of a variety of methods in sanitary education and employee training.

생물고분자 막 형성을 이용한 동물세포 배양 및 단클론항체 생산 (Animal Cell Culture and the Production of Monoclonal Antibody(MAb) Using Biopolymer Membrane)

  • 손정화;유선희;김성구
    • KSBB Journal
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    • 제13권1호
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    • pp.13-19
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    • 1998
  • Biopolymer membrane was prepared using two oppositely charged natural biopolymers. The biopolymer membrane was used for the encapsulation of two hybridoma cell lines(ATCC CRL-1606, ATCC HB-8852) to produce monoclonal antibodies. In order to reduce the down stream steps, the pre size of the membrane was controlled to retain the monoclonal antibodies in the capsules based on the diffusion experiments with standard proteins. T-flask culture showed cell densities of 8$\times$107 cells/mL and 3$\times$107 cells/mL, and MAb concentrations of 506$\mu$g/mL and 109$\mu$g/mL for encapsulated ATCC CRL-1606 and HB-8852, respectively. Two liter perfusion cultures with encapsulated ATCC HB-8852 were performed to enhance the MAb production. The MAb production of the encapsulated hybridoma increased considerably comparing to the culture using silicon tubing for oxygen transfer.

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Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide

  • Pham, Tuan Anh;Kim, Jeong-Jun;Kim, Keun
    • Mycobiology
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    • 제38권2호
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    • pp.137-143
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    • 2010
  • The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, $25^{\circ}C$ culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of $10^7$ conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.

수도작에서 왕우렁이 및 오리 방사가 수량구성요소에 미치는 영향 (Influence of Pond-Snail and Duck Pasture on the Yield Components of Paddy Rice)

  • 정순재;정원복;정대수;최봉출;오주성
    • 한국유기농업학회지
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    • 제8권2호
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    • pp.79-87
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    • 2000
  • This experiments was carried out to find the effect of different organic farming types, the Duck-pasture plot, the Pond-snail pasture plot andd control of the conventionalculture plot farming on the yield and its components of paddy rice. The results obtained are summarized as follows. Plant height an Tillering were significantly increase in the Duck-pasture plot. Panicle number pr hill, NO. of grain per spike and 1,000grain weight were significantly increase in the conventional culture plot. Yield pre 10a were 574.13kg at pond-snail pasture plot, 576.81kg at Duck-pasture plot and 579.04kg at conventional culture plot. From these results, it seems that the organic farming are useful and pasture of the Duck, Pond-snail for depression of the weed occurrence or increase of yield per 10a is effective as the environmental perservation farming.

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A Study on Metaverse Culture Contents Matching Platform

  • Kim, Jeong-Gwon
    • International Journal of Advanced Culture Technology
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    • 제9권3호
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    • pp.232-237
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    • 2021
  • Pre-Corona cultural content was largely formed face to face. For example, the service was great while visiting tourist attractions, visiting performances to see K-Pop, or visiting lectures to learn K-Beauty. However, after the coronation, the virtual world-centered Metaverse began to take the spotlight as non-face-to-face services changed. IT and cultural contents have been combined and developed into various digital services. In that sense, Metaverse, which breaks the boundaries between real-world space and virtual space, has the potential to escape from physical time and space constraints. However, Korea still lacks a Metaverse platform for cultural content. In particular, it is necessary to match each other's necessary services by providing Metaverse -oriented cultural contents that users want, including experienced cultural contents such as K-pop and K-beauty, away from cultural contents that are developed around history museums. Therefore, in this paper, we would like to propose a Metaverse-based cultural content matching platform where users can experience cultural content directly or indirectly.

Adventitious Shoots Regeneration from Seed Explants of Xanthoceras sorbifolium

  • Hyunseok Lee
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2020년도 추계국제학술대회
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    • pp.58-58
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    • 2020
  • Xanthoceras sorbifolium Bunge (yellowhorn) is a woody tree in the soapberry family, Sapindaceae, native to northern China. This species has been identified as a major woody bioenergy plant for bio-diesel production because of high oil content in seed. But the flowers do not bear fruit well while the many flowers blooming. This study was performed to regenerate in vitro plantlet using adventitious shoot formation. To establish the protocol of plant regeneration, adventitious shoots formation rate in the culture of cotyledon of immature zygotic embryos was 68.6% in 1/2 MS medium with 0.5 mg l-1 BA and 3% sucrose (w/v). In the culture of cotyledons of mature zygotic embryos, induction of adventitious shoots was needed to contain high sucrose in pre-culture medium and the frequency of shoot induction was 64.4%. Multiple shoots were induced in 0.5 mg l-1 TDZ, and rooting of shoot was induced 4.0 mg l-1 IBA. Flow cytometry analysis revealed that all the regenerated plantlets were diploid.

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Mixed Methods Research on the Intercultural Sensitivity of Summer Program Participants; Comparison of US Participants and Korean Language Partners

  • Soyoung Jeong
    • International Journal of Advanced Culture Technology
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    • 제11권4호
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    • pp.112-122
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    • 2023
  • This study was conducted as part of a summer Korean language and culture program at a university in Korea, aimed at examining the changes in intercultural sensitivity among American students who participated in the program, as well as the Korean students who served as their language partners. The research utilized a mixed-method approach combining qualitative and quantitative analysis. To derive research findings, pre- and post-program Intercultural Sensitivity Scale surveys were administered to participating students and semi-structured interview data were collected from a randomly selected group of six students. The research findings indicated that among a total of twenty-seven students including both American and Korean students, significant changes in intercultural sensitivity were observed in the domain of Interaction Engagement. Furthermore, fourteen American students showed significant changes in their Interaction Confidence, while the Korean students who participated as language partners did not exhibit significant changes in the five subdomains of intercultural sensitivity.

Induction of Ginseng Hairy Roots And Their Possible Application To Large Scale Culture

  • Yang, Deok-Chun
    • Plant Resources
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    • 제6권1호
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    • pp.1-6
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    • 2003
  • Ginseng(Panax ginseng C. A. Meyer) is important medicinal plant but requires 4-year cultivation for root harvest because of slow growth. In contrast, ginseng hairy roots induced by introducing Ri-plasmid of Agrobacterium rhizogenes into genomic DNA of plant cells show vigorous growth, and the hairy roots produce the same or more saponins than natural ginseng roots. Therefore, hairy roots can be used for commercial purposes. The present study was carried out to induce hairy roots with both active growth and high saponin contents. Numerous hairy roots of Panax ginseng were obtained after root disks of three-year old roots were infected with Agrobacterium rhizogenes R1000 A4T in dark condition after one month of culture. About 3 hundred lines of hairy roots were selected according as morphological characters on medium with carbenicillin. After pre-selection of fifteen lines of hairy roots with active growth, KGHR-l and KGHR-8 lines were finally selected which had characters of high content of ginsenoside-Rd and ginsenoside-Re, respectively. The optimum growth of hairy roots was achieved in the culture of 1/2 MS liquid medium in dark (22 $^{\circ}C$) under 60 rpm gyratory shaking. Hairy roots grew well in 5L Erlenmeyer flasks, lL roller drums, 10L jar-fermenters, and especially in 20L air-lift culture vessels.

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희귀 및 멸종위기 식물 미선나무(Abeliophyllum distichum Nakai)의 절편위치 및 치상방법에 따른 기내증식 및 미세삽목 (Effect of explant's position and culture method on shoot proliferation and micro-cuttings for a rare and endangered species, Abeliophyllum distichum Nakai)

  • 이나념;김지아;김용욱;최용의;문흥규
    • Journal of Plant Biotechnology
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    • 제42권3호
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    • pp.228-234
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    • 2015
  • 본 실험은 희귀 및 멸종위기 식물인 미선나무의 효과적인 증식을 위하여 줄기 증식, 생장 및 발근에 미치는 절편의 위치 효과 및 치상 방법의 효과를 구명하기 위해 실시되었다. 줄기유도는 BA를 처리한 액아절편 배양이 효과적이었으며 절편 당 2.4개의 줄기가 유도되었다. 정아 및 액아절편을 BA 1.0 mg/L를 전처리하여 수평과 수직으로 치상하였을 때 줄기유도는 액아 절편의 수직치상으로 절편 당 2.5개로 가장 좋았다. 반면 줄기의 생장, 발근 및 뿌리 발달은 정아절편의 수직치상에서 양호하였고 BA의 전처리 시간이 길어질수록 발근이 억제되는 경향을 보였다. 기내증식된 줄기의 정아 및 액아를 절편으로 기외에서 미세삽목(micro-cutting)을 실시하여정아절편에서 100% 발근되었고, 뿌리수는 개체 당 평균 6.2개로 가장 양호하였다. 미세삽목묘는 온실에서 95% 이상 순화되었다. 이상의 결과로 볼 때 미선나무의 기내 증식은 액아를 절편으로 BA 처리 후 수직 치상하는 방법이 효과적이며, 줄기의 생장 및 발근은 정아를 절편으로 수직치상 하는 것이 효과적으로 나타났다. 또한 정아를 절편으로 미세삽목을 통해 효과적인 발근 유도 및 순화가 가능하여 미선나무의 실용적인 묘목생산이 가능함을 보여주었다.