• 제목/요약/키워드: Pre/Post Process

검색결과 651건 처리시간 0.025초

Post-transcriptional and post-translational regulation during mouse oocyte maturation

  • Kang, Min-Kook;Han, Seung-Jin
    • BMB Reports
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    • 제44권3호
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    • pp.147-157
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    • 2011
  • The meiotic process from the primordial stage to zygote in female germ cells is mainly adjusted by post-transcriptional regulation of pre-existing maternal mRNA and post-translational modification of proteins. Several key proteins such as the cell cycle regulator, Cdk1/cyclin B, are post-translationally modified for precise control of meiotic progression. The second messenger (cAMP), kinases (PKA, Akt, MAPK, Aurora A, CaMK II, etc), phosphatases (Cdc25, Cdc14), and other proteins (G-protein coupled receptor, phosphodiesterase) are directly or indirectly involved in this process. Many proteins, such as CPEB, maskin, eIF4E, eIF4G, 4E-BP, and 4E-T, post-transcriptionally regulate mRNA via binding to the cap structure at the 5' end of mRNA or its 3' untranslated region (UTR) to generate a closed-loop structure. The 3' UTR of the transcript is also implicated in post-transcriptional regulation through an association with proteins such as CPEB, CPSF, GLD-2, PARN, and Dazl to modulate poly(A) tail length. RNA interfering is a new regulatory mechanism of the amount of mRNA in the mouse oocyte. This review summarizes information about post-transcriptional and post-translational regulation during mouse oocyte meiotic maturation.

초등과학 영재의 물체 인식 개념 변화를 위한 튜토리얼의 개발과 적용 (Development and Application of Tutorial for Conceptual Change on Object Recognition of Scientific Gifted in Elementary School)

  • 이지원;김중복
    • 한국초등과학교육학회지:초등과학교육
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    • 제30권3호
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    • pp.340-352
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    • 2011
  • The purpose of this study was to analyze effects of teaching materials for elementary science gifted conception about object recognition. Elementary science gifted have misconceptions that they can see in lightness. They can not explain how a shadow is made. This paper reports in-depth investigation on elementary science gifted's understanding of object recognition focusing on process of light. A program is developed to elementary science gifted in the subject matter. The tutorial emphasizing the process of light consists of pre-test, worksheet, and post-test. The Tutorial has 4 steps; darkness and light, light on things, light reached eyes, structure of the eyes. Each steps has 2~4 experiments. Through the tutorial, we expect their misconceptions can be changed into scientific conceptions. For the research and analysis, a questionnaire is posed to 39 elementary science gifted at M Elementary School in D Metropolitan City. The first method of product analysis makes a comparative study of pre-test, post-test score, and hake gain each test. As a result, total score of all student was raised. And hake gain of pre-test(II) is 0.6, hake gain of post-test is 0.68. It is Medium gain. Also, elementary science gifted could understand how we see through the tutorial emphasizing process of light. And their misconceptions can be changed into scientific conceptions.

Process Development for the Enzymatic Hydrolysis of Food Protein: Effects of Pre-treatment and Post-treatments on Degree of Hydrolysis and Other Product Characteristics

  • Chae, Hee-Jeong;In, Man-Jin;Kim, Min-Hong
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제3권1호
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    • pp.35-39
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    • 1998
  • An enzymatic process was developed to produce protein hydrolysater form defatted soya protein. Various unit operations were tried, and the effects of pre- and post-treatments on the product characteristics such as degree of hydroylsis (DH), free amino acid content (%FAA) and average molecular weight (MW) were investigated. The use of acid washes showed no difference in %DH. Increasing pH during pre-cooking gave lower %DH. Alkaline cooking made too much insoluble protein, thus the protein yield was too small. A better hydrolysis with more acceptable taste was obtained when the combination of Neutrase/Alcalase/Flavourzyme was used in place of Alcalase/Flavourzyme combination; Untoasted defatted soya was more effective on the proteolysis than toasted one. The MW of the evaporated and spray dried product was higher than that of undried product, due to precipitation of low-solubility components. When ultrafiltration and the product concentration carried out the product separation by reverse osmosis, the solubility and the taste of the product were improved. The difference between enzyme hydrolysate and acid hydrolysate was significant in free amino acid composition, especially in tyrosine, phenylalanine, glutamine and asparagine.

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GIS를 활용한 새만금 환경 DB 구축 (Construction of Environment Database for Saemangeum watershed Using GIS)

  • 엄명철;조국현;이광야;김계현
    • 한국농공학회:학술대회논문집
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    • 한국농공학회 2002년도 학술발표회 발표논문집
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    • pp.381-384
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    • 2002
  • The purpose of this study is to construct an Environment Database Management System (EDMS) for the Saemangeum watershed based on the linkage of Water Quality Model, i.e. QUALKO and WASP model, and GIS database to estimate water quality effectively in this area. There are two major river systems on this study area, the Mangyeong and the Dongjin rivers. Input data are automatically generated through the calculation of the pollutant loading and inflow concentration from the point and non-point sources. The developed system is composed of three different phases, such as pre-process, model performance and post-process. The Model performance is supported by the database at pre-process phase and model performance results were shown in the graphs and attribution data at post-process phase. The measured data from the Mangyeong and the Dongjin rivers are used to evaluate the applicability of EDMS. The EDMS shows higher reliability, and it is expected to contribute to the effective management and improvement of water quality modeling in the Saemangeum watershed since the system reduces complications of using a model in DOS operating environment and increases the accuracy of water quality analysis.

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핵심기본간호술 교육프로그램이 핵심기본간호술 수행능력, 임상수행능력 및 문제해결과정에 미치는 효과 (The Effects of Core Fundamental Nursing Program on Core Fundamental Nursing Skills Competency, Clinical Competency and Problem Solving Process)

  • 김영희
    • 한국산학기술학회논문지
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    • 제19권9호
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    • pp.362-373
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    • 2018
  • 본 연구의 목적은 졸업반 간호학생에게 한 학기 동안 적용한 핵심기본간호술 교육프로그램이 핵심기본간호술 수행능력, 임상수행능력 및 문제해결과정에 미치는 효과를 규명하고자 하는 것이었으며 단일군 사전사후 설계의 원시실험연구였다. 핵심기본간호술 교육프로그램은 3단계로 구성하였으며, 총 12회기로 운영하였다. 연구대상자는 일개 간호대학의 졸업학년 총 192명이었으며 2017년 3월 20일부터 6월 23일까지 실시하였다. 자료는 AMOS 20.0과 S AS 9.3 프로그램을 이용해 기술통계와 paired t-test, t-검정, ANOVA로 분석되었다. 연구결과로, 핵심기본간호술 교육프로그램에 대한 사전사후 인식으로 수기능력 증진 도움 여부는 사전 $(3.42{\pm}.42)$점, 사후 $3.88({\pm}.61)$점, 임상적응력증진 도움 여부는 사전 $3.74({\pm}.47)$점, 사후 $(3.98{\pm}.67)$점으로 나타났다. 핵심기본간호술 수행능력(p=.017)과 임상수행능력(p=.041)은 핵심기본간호술 교육프로그램 후에 통계적으로 유의하게 증가하였다. 대상자의 사후 핵심기본간호술 수행능력은 남성 군(p=.003), 간호학전공 만족도가 매우 만족한 군(p=<.000), 핵심경진대회 참여 목적은 자신감 향상 군(p=.044)에서, 사후 임상수행능력은 남성 군(p=.013), 간호학전공 만족도가 매우 만족한 군(p=.009), 핵심경진대회 참여 목적은 자신감 향상 군(p=.047), 사후 문제해결과정은 남성 군(p=.005), 간호학전공 만족도가 매우 만족한 군(p=<.000), 핵심경진대회 참여 목적은 자신감 향상 군(p=.004)에서 유의한 차이가 있었다. 결론적으로 이 연구는 졸업반 간호학생의 핵심기본간호술 교육프로그램이 핵심기본간호술 수행능력 및 임상수행능력에 긍정적인 영향을 미친 것으로 나타났다.

현실요법에 기초한 미술치료 프로그램이 대학생의 체중조절과 신체상 및 자아존중감에 미치는 효과 (The Effect of an Art Therapy Program based on Reality Therapy on Weight Control, Body Image, and Self-esteem of College Students)

  • 김원경;박정희
    • 한국학교보건학회지
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    • 제14권2호
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    • pp.249-268
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    • 2001
  • This study was carried out to identify the effects of an art therapy program based on reality therapy on the weight control, body image, and self-esteem of college students. The program was developed by the authors. The design was a pretest-post test control group design. The subjects were college students attending M college : 8 in experimental group A, 6 in experimental group 2, who wanted to control their body weight and 16 in the control group who didn't attend the program those in the experimental group attended the program once (group A) or twice (group B) per week meeting a total of six times. The data were analyzed by quantity method (SPSS 10.0 program) and quality method(process analysis). The results were as follows : 1. The average obesity index of pre-test, post-test, and a follow-up test decreased from 8.9% to 6.1%, 3.7%. And the rate of decrease both pre-post and post-follow up were statistically significant 2. The average obesity index of group A decreased from 9.6% to 6.3%, 4.1% and the rate of decrease between pre and post were statistically significant. But the rate of decrease between post and follow-up were statistically not significant 3. The average obesity index of group B decreased from 7.8% to 5.8%, 3.1% and the rate of decrease between pre and post were not significant statistically. But the rate of decrease between post and follow-up were statistically significant 4. The average scores of pre and post body image increased and were statistically significant : group A from 129.0 to 153.5, group B from 127.7 to 155.2. 5. The average scores of pre and post semantic differential method increased and were statistically significant: group A from 38.1 to 44.1, group B from 38.0 to 45.0. 6. The average scores of pre and post self-esteem increased: group A increased from 37.3 to 40.3 and were statistically significant, group B increased from 36.5 to 39.5 but was statistically insignificant 7. The average scores of pre and post obesity rate, body image, semantic differential method and self-esteem in the control group were not statistically significant 8. The pre and post scores of body image(p=.001), semantic differential method(p=.001) and self-esteem(p=.039) between experimental and control group were statistically significant. 9. In Duncan multiple comparison, it was not statistically significant between group A and B, but statistically significant between group A and the control group, group B and the control group in obesity index, body image, semantic differential method. There was no statistically significance among the three groups in self esteem 10. In Duncan multiple comparison, group A gained higher scores than group B in obesity index(-1.2%), and self esteem(0. 3). Group B gained higher scores than group A in body image(3. 0), and semantic differential method(1. 0). So it is difficult to conclude whether group A or B is more effective. In conclusion, the art therapy program based on reality therapy was effective in weight the control program of college students: obesity was decreased, the scores of body image and semantic differential method and self-esteem were increased. The scores of group A were increased more than group Band were statistically significant, The time interval was a very important factor in this program. The recommendation is : the validity of this program has to be tested through various subjects and in various areas. And also this program has to be tested in other topics.

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해저케이블 작업에서의 KP와 XTE 구현을 위한 최적화 알고리즘 (Optimization Algorithm for KP and XTE Implementation on the Submarine Cable Work)

  • 이태오;임재홍
    • 한국정보통신학회논문지
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    • 제7권3호
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    • pp.423-436
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    • 2003
  • 해저 광케이블 공사는 해양조사, PLGR(Pre Lay Grapnel Run), 천해부공사, 포설/매설 순서로 이루어진다. PLGR은 해저 광케이블 매설전에 루트상의 해양 오염물(로프, 와이어, 그물, 어망 등)을 사전에 제거하는 작업이다. 이러한 PLGR은 케이블의 해저면 안착을 용이하게 하고, Plough 및 ROV(Remotely-Operated Vehicle) 매설작업의 성능을 향상시키며, 매설장비를 보호하기 위한 작업이다. 본 논문은 해양조사, PLGR 작업을 효율적으로 관리하기 위한 항해 누적 거리(KP Kilometer Post)와 경로 이탈 오차(XTE : Cross Track Error)의 최적화 알고리즘 구현에 대해서 논한다. 본 논문에서는 이를 위하여 전체적인 PLGR 작업을 구성하고, 항해 누적 거리와 경로 이탈 오차의 최적화 알고리즘을 제안하였다. 그리고 현재 진행중인 PLGR 작업의 일부 루트를 따라서 KP와 XTE 결정 알고리즘을 실험하였다.

인터넷 쇼핑몰의 사용자 채택에 대한 구매 단계별 영향 요인 (The Impact on the User Acceptance of Internet Shopping Malls by Purchase Stages)

  • 오창규
    • 한국정보시스템학회지:정보시스템연구
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    • 제15권4호
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    • pp.125-147
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    • 2006
  • Internet shopping mall has dual nature of information technology features defined by the web-based application system and marketing features explained by the shopping marketplaces. The purpose of this study aims at analyzing the consumer's attempt to purchase and re-purchase intention to the internet shopping mall to combine the IT perspective and the marketing perspective. This paper develop the integrated research model with the acceptance behaviors of customers through consumer's purchase decision process(pre-purchase stage and post-purchase stage). The results from an empirical survey as follows: First, the integrated model is valid in predicting the acceptance of the internet shopping malls by using structured equation modeling. Second, there are the differences between pre-purchase stage and post-purchase stage by each conceptual constructs. Finally, the results of MSEM(Multi-group Structured Equation Modeling) indicate the perceived ease of use and perceived trust to the attitude to the internet shopping mall is more effectively predicted at the pre-purchase stage. Vice versa, the perceived usefulness to the intention to the purchase intention of internet shopping mall is more effectively predicted at the post-purchase stage. This study provides an integrative approach to develop the effective and successful internet shopping mall strategy.

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Gel Color and Texture of Surimi-like Pork from Muscles at Different Rigor States Post-mortem

  • Kang, Geun-Ho;Yang, Han-Sul;Jeong, Jin-Yeon;Moon, Sang-Hoon;Hur, Sun-Jin;Park, Gu-Boo;Joo, Seon-Tea
    • Asian-Australasian Journal of Animal Sciences
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    • 제20권7호
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    • pp.1127-1134
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    • 2007
  • Gels were made from surimi-like pork (SLP) made from muscles obtained at 1, 24 and 72 h post-mortem. The SLP from pre-rigor muscle had higher pH and moisture percentage compared to in- or post-rigor muscles. Also, SLP from pre-rigor muscle showed higher concentration of water-soluble protein that was washed out during the process. Gel from post-rigor muscle exhibited higher a* and b* value, and also resulted in higher Chroma and lower hue values. The dark color of gel from post-rigor muscle was related to higher concentration of sarcoplasmic protein in SLP and denser structure in the gel matrix. SDS-PAGE showed higher intensity of the phosphorylase in the sarcoplasmic protein fraction from pre-rigor muscle. Gel from post-rigor muscle showed higher hardness and sensory firmness, and the greater firmness was related to higher concentration of protein in SLP, and a compact network with smaller pockets in the gel matrix.

환경쟁점분석 수업이 초등학생의 환경의사결정 능력에 미치는 영향 (The Effects of Environmental Issue Analysis Instruction on Elementary School Students' Environmental Decision Making Ability)

  • 민은홍;최돈형
    • 한국환경교육학회지:환경교육
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    • 제20권1호
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    • pp.90-105
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    • 2007
  • The purpose of this study is to find the influence of environmental issue analysis instruction on the environmental decision making ability for grade 5 elementary school students. The study was done through pre and post testing control group structure. The object of this study is grade 5 of I elementary school students which were divided into 35 student test group and 54 student control group. Through studying references, the selection standard of appropriate environment issue and the environmental issue analysis instructing objective. Conducted the environment issue instructing based on the selected environment issue and instructing objective. The classes were held in total of 6 sessions in the chapters related to class objective and class content within the curriculum. The pre and post testing was done using environment decision making ability test sheet which was reconstructed by myself and the results were analyzed by t-test. As a result of comparing pre and post testing the students in test group showed significant results in the processes of problem recognition, evaluation of alternatives, behave planing (p<.001). As a result of comparing the differences of environment decision making ability of pre and post test of test group and control group, it showed significant results in the process of evaluation of alternatives(p<.00l). The environment issue analysis class has positive influence on the environment decision making abilities of the students but since the outcome of environment decision making ability is lower, there is a need for long term environment education plan and further studies to find whether the environment issues within the textbook is appropriate in the elementary student level, useful school aspect and the influence of environment issue analysis class on the change of values for individuals.

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