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Analysis of Chemical and Physical Characteristics of Log Woods for Oak Mushroom Production Depending on Cultivation Periods and Steam Explosion Treatment (표고버섯 골목의 사용연수에 따른 화학적, 물리적 성상 및 폭쇄처리 후 변화 관찰)

  • Koo, Bon-Wook;Park, Jun-Yeong;Lee, Soo-Min;Choi, Don-Ha;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.1 s.129
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    • pp.77-86
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    • 2005
  • In order to investigate the ability of log wood for oak mushroom production as a source of an alternative energy, both chemical and physical characteristics of log wood were investigated according to the cultivation periods. Also, both chemical and physical characteristics of material that treated by steam explosion were investigated to confirm the pretreatment effect by remaining enzyme as a control. The contents of ash, water-, alkali- and organic soluble extracts have been increased after the inoculation. It appeard that holocellulose contents substantially decreased and the contents of lignin as another main component of wood remained constant after the inoculation. However this result implied that indeed, a sufficient amount of lignin has been degraded paritially by enzymes of oak mushroom Lentinus edodes if we consider that the amount of holocelulose was substantially reduced. It also indicated that the degree of degradation gradually progressed but crystallinity decreased after the inoculation. The contents of water-, alkali- and organic soluble extracts have been increased by steam explosion. Holocellulose contents increased within narrow limits and lignin contents remained constant. However the contents of holocellulose and lignin have been decreased by steam explosion, considering that the amount of other extractives was relatively increased. The degree of crystallinity and lignin contents reduction by steam explosion was almost similar to the result obtained by increasing cultivation periods. According to the results, log woods for mushroom production have a potential as material for developing alternative energy.

Cosmeceutical Activity of Broccoli (Brassica oleracea var. italica Plenck) with Different Light Sources (광원별 브로콜리 추출물의 화장품약리활성 검증)

  • Lee, Soo-Yeon;Jun, Hye-Ji;Yoon, Ji-Young;Kim, Tae-Su;Park, So-I;Lee, Sung-Pyo;Park, Joo-Hoon;Lee, Jin-Young
    • Journal of Life Science
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    • v.22 no.3
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    • pp.347-353
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    • 2012
  • The purpose of this study was to research the cosmeceutical activity of 70% ethanol extracts when Brassica oleracea var. italica Plenck (BO) was dried under red lighting (RLD), blue lighting (BLD), red+blue lighting (RBLD), or white lighting (WLD). The electron-donating abilities of 70% ethanol extracts from drying BO under RLD, BLD, RBLD, and WLD were 62.8%, 68.1%, 60.9%, and 69.9%, respectively, at a concentration of 500 ppm. The superoxide dismutase (SOD)-like activities of 70% ethanol extracts from drying BO under RLD, BLD, RBLD, and WLD were 39.2%, 47.2%, 19.6%, and 21.6%, respectively, at a concentration of 1,000 ppm. In terms of xanthine oxidase inhibition effects, the proportions of 70% ethanol extracts after drying BO under RLD, BLD, RBLD, and WLD were 45.1%, 56.2%, 38.8%, and 53.3%, respectively, at a concentration of 1,000 ppm. The tyrosinase inhibition effects of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 21.0%, 13.1%, 26.0%, and 19.1%, respectively, at a concentration of 1,000 ppm. The collagenase inhibition effect of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 47.4%, 20.8%, 54.6%, and 37.2%, respectively, at a concentration of 1,000 ppm. The astringent inhibition effects of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 21.5%, 39.6%, 40.0%, and 51.6%, respectively, at a concentration of 5,000 ppm. All of these findings suggest that BO extracts dried under RLD, BLD, RBLD, and WLD have great potential as a cosmeceutical ingredient with good biological activity.

Problems in Quantification of Adequacy of Academic Library Collections -Critical Analysis of Standards for Academic Libraries in the U.S.- (종합대학 도서관장서의 적정량기준 설정에 관한 고찰 -미국의 종합대학도서관기준을 중심으로-)

  • Chung Young Sun
    • Journal of the Korean Society for Library and Information Science
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    • v.8
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    • pp.183-207
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    • 1981
  • Library standards have been the source of considerable controversy, whereas many problems are involved in developing stardard for university library collections. For evaluation purposes, standards should be precise, quantifiable and measurable. In the United States, however, standards for academic libraries are limited to qualitative statements and principles. Quantitative standards, when given, are ususally related to the number of population in the institution being served, or the prescribed quantitative objectives are often arbitrarily formulated by value judgements. The study in this paper attempts to explain the problems involved in developing quantitative standard for academic library collections. Two problems facing in the formulation of the optimal size of collection are identified. One is the theoretically faulty concept of adequacy of collection to meet the situations of diversity of university libraies, and the other is the difficulties in quantification and measurement, along with the lack of concept of adequacy of collection. However, quantification of adequate size of collection is proved to be useful on the pratical level, even though not valid theoretically. ACRL, Clapp/Jordan and Voigt developed formulas or models for setting the optimal size of a library collection for any particular university library. The main purpose of this study is the analysis of the above formulas. ACRL standard was drawn from obervation and analysis of statistcs in leading library collections. In academic field, this judgement appears to have been based on the assumption that a high-grade institution would be apt to have a good library collection. This study criticizes ACRL standard for its failure to include some determinants of measurements, and points out the limitations of the standard. In contrast. Clapp/Jordan developed a formula rather scientifically based upon bibliographical sources. This is similarly empirical but has the advantage of bringing into play the elements which make universities diverse in nature. Both ACRL and Clapp/Jordan formulas share two major defects. (1) the specific subject needs of the collection are not indiacted directly, and (2) percentage rate of growth is an indicator in measuring the potential utility of a collection. Thus both formulas failed to provide a basis for meaningful evaluation. Voigt further developed a model for determining acquisition rates for currently published materials based on bibliographic technique. Voigt model encourages experimentation with different programs and different allocations of input resources, designed to meet the needs of the library's particular population. Standard for university library collections can be formulated in terms of input(traditional indicator), or additionally, in terms of output(cost-effectiveness). Cost effectiveness is expressed as user satisfaction, ability to provide wanted materials within a reasonable time period. Thus simple quantitative method does not cover all the situations of diversity of university library collections, nor measures the effectiveness of collections. Valid standard could not be established without further research.

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Hospice volunteer's Attitude Toward Care of the Dying (호스피스 자원 봉사자들의 말기 환자 돌봄에 대한 태도)

  • Lee, Mi-Ra;Lee, Won-Hee
    • Journal of Hospice and Palliative Care
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    • v.4 no.1
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    • pp.57-67
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    • 2001
  • Purpose : The purpose of this study was to identify attitudes of hospice volunteers toward care of for terminally ill patients. Method : This was a descriptive study with a sample of 84 adults who were registered for a hospice volunteer education program at Severence Hospice Center. The Frommelt (FATCOD) scale on attitudes toward the care of the dying (Cronbach alpha=.778) and an open ended questions on "what if you only have 6 months to live" were used to collect the data. The data was analyzed using SPSS/W and content analysis. Results : 1) The hospice volunteers were mostly female, with an average age of 45 years, half of them were college graduates and their religious preference was Protestant. 2) The participants of this study demonstrated positive attitudes to care for the dying which is in coherence with hospice philosophy and principles. However they indicated difficulties in maintaining close relationships with people who are dying, and in communicating and sharing, and encouraging those who are dying to express their feelings. In the open ended questions, they identified that their most important issues would be guilt feelings toward their children, family concerns, and the burden of unfinished business in their lives. They also identified the fear of pain in the dying process and fear of the afterlife. The care they would like to receive was to have peace of mind, have a good listener, spiritual counselling, and pain relief and to be respected as a human being. The source of strength would be faith in God and they would like to overcome their of dying. The FATCOD scale has limitation in describing and identifying the need and attitude toward the care of the dying revisions were made. Conclusion : We all are the potential clients for the hospice rare. In a hospice volunteer education program, communication and interpersonal skill are essential. The fear of dying, afterlife, concerns about family with children, and human dignity are major concerns in hospice and palliative care.

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Qualitative Enzyme-Linked Immunosorbent Assay (ELISA) for the Diagnosis of Edwardsiellosis (Edwardsiellosis의 진단을 위한 정성적 ELISA법)

  • Kim, Myoung-Sug;Hwang, Eun-A;Huh, Min-Do;Jeong, Hyun-Do
    • Journal of fish pathology
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    • v.12 no.1
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    • pp.24-31
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    • 1999
  • Optimization and standardization of solid phase enzyme immunoassay were done for the diagnosis of edwardsiellosis in fish. The analyzed degree of immobilized antibody on surface of solid phase with peroxidase saturation method showed the optimized result by using partially purified $50{\mu}g/ml$ of rabbit anti-E. tarda Edk-2 antibody in sodium bicarbonate buffer for overnight incubation to cover the surface of polystyrene beads. Optimized immunoreaction was observed in the treatment of $50{\mu}g/ml$ of biotin conjugated antibody followed extravidin-peroxidase diluted 1 : 2,000 in PBS. The detectable concentrations of the this method were $1{\times}10^5$ cells/ml and $1{\times}10^5$ cells/ml expressed as the source of antigen amount for EDTA extraction and heat extraction, respectively. High cross-reaction of solid phase ELISA with the prepared rabbit and-E. tarda Edk-2 was observed against E. tarda strains isolated from flounder suffering from edwardsiellosis in aquatic farms of Korea. It suggested that the potential of this solid phase of ELISA technique is very powerful for the application to different strains of E. tarda isolated in farms of many different areas.

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Increased whole grain, fruits and vegetable intake reduced oxidative stress in high school students (전곡류 및 채소, 과일 섭취의 증가가 청소년의 체내 항산화능 개선에 미치는 영향)

  • Kim, So Hyeon;Cho, Sang Woon;Hwang, Seong Su;Ahn, Mijung;Lee, Dukhee;Kang, Seung Wan;Park, Yoo Kyoung
    • Journal of Nutrition and Health
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    • v.45 no.5
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    • pp.452-461
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    • 2012
  • There is increasing evidence that dietary factors in plant-based diets are important for the prevention of chronic disease. Especially, phytonutrients in fruits and vegetables have been recognized as major contributors for the decreased level of oxidative stress. In this study, the effect of switching the dietary habit to high consumption of fruits and vegetables were evaluated on the parameters of serum antioxidant status in healthy high school students. Forty one students participated in a randomized controlled trial and were assigned to the control group (n = 18) or the intervention group (n = 23). The intervention group was provided for 8 weeks with the main food source being whole grain and vegetables. Anthropometric measurements, blood parameters and dietary intakes were measured, and compared before and after study. After 8 weeks, weight and BMI were significantly decreased in the intervention group (p = 0.000). The serum diacron reactive oxygen metabolites (d-ROMs) test resulted in a significantly decreased level only in the intervention group (p < 0.05) after 8 weeks, but serum biological antioxidant potential (BAP) was increased significantly in both groups (p < 0.001). Intake of energy, total fat, cholesterol and sodium in the intervention group were significantly decreased after 8 weeks (p < 0.05). Also, the intervention group had significantly increased vitamin and phytonutrient intakes of all-trans-${\beta}$-carotene, ${\alpha}$-carotene, ${\alpha}$-tocopherol, ascorbic acid, and total phenols (p < 0.05). Overall, the results of this study suggest that whole grain, fruits, and vegetables supplementation showed improvement of the adolescent health.

Antioxidant activities from the Different Parts of Artemisia argyi H. using an in vitro System (In vitro 시스템에서 남해 약쑥(Artemisia argyi H.)의 부위별 항산화 활성)

  • Ha, Gi-Jeong;Jeong, Chang-Ho;Jeong, Hee-Rok;Heo, Ho-Jin;Shon, Gil-Man;Rho, Chi-Woong;Kim, Nak-Ku
    • Journal of agriculture & life science
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    • v.45 no.1
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    • pp.109-117
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    • 2011
  • The antioxidant activities of 60% ethanol extracts from different parts (leaf, stem, and root) of Artemisia argyi using an in vitro system were examined to find a possibility as natural antioxidant substances. The highest total phenolics (23.08 mg/g) was obtained by 60% ethanol extract of leaf. The scavenging activity of 60% ethanol extract from different parts of A. argyi on the DPPH and ABTS radical was raised with increasing amount of extracts, and 60% ethanol extract from leaf showed the highest radical scavenging activities. The leaf extract presented the highest reducing power and strong inhibitory effect on autooxidation of linoleic acid. In MDA formation using mouse brain homogenates, the 60% ethanol extracts of A. argyi also exhibited antioxidant activity as inhibition of MDA (71.38% at $200{\mu}g/mL$). Therefore, these results suggest that the 60% ethanol extract of A. argyi leaf possess excellent antioxidant activities and thus it has great potential as a source for natural antioxidant.

Antioxidant and Neuronal Cell Protective Effect of Purple Sweet Potato Extract (자색고구마 추출물의 항산화 효과 및 신경세포 보호효과)

  • Kwak, Ji-Hyun;Choi, Gwi-Nam;Park, Ju-Hee;Kim, Ji-Hye;Jeong, Hee-Rok;Jeong, Chang-Ho;Heo, Ho-Jin
    • Journal of agriculture & life science
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    • v.44 no.2
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    • pp.57-66
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    • 2010
  • The antioxidant and neuronal cell protective effects of water extract from purple sweet potato were investigated. The total phenolics and monomeric anthocyanin contents of purple sweet potato extract were 44.25 mg/g and 2,394 mg/L, respectively. The antioxidant activities of purple sweet potato extract were evaluated using various antioxidant tests, including 1,1-diphenyl- 2-picrylhydrazyl (DPPH), 2,2'-azino- bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, ferric reducing/antioxidant power (FRAP) and reducing power. In these assays, the extract of purple sweet potato presented significant radical scavenging activities, FRAP, and reducing power in a dose-dependent manner. MTT {3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl- tetrazoliumbromide} reduction assay showed significantly increase in cell viability when PC12 cells were pretreated with purple sweet potato extract. Because oxidative stress is also known to increase neuronal cell membrane breakdown, we further investigated by lactate dehydrogenase (LDH) and neutral red uptake assay. Purple sweet potato extract inhibited oxidative stress-induced membrane damage in neuronal cells. Therefore, these data results demonstrated that water extract of purple sweet potato have antioxidant activity and neuronal cell protective effect thus it has great potential as a natural source for human health.

Screening for Antioxidant Effects of Aerial Part Extracts Obtained from Sixteen Compositae Species (국화과 식물 16종 지상부 추출물의 항산화효과 탐색)

  • Woo, Jeong Hyang;Shin, So Lim;Chang, Young Deug;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.271-278
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    • 2009
  • The attempts to develop natural antioxidants have been made with aerial part of 16 Compositae species by analyzing their phenolic compound contents, scavenging activities on 1,l-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, ferrous ion chelating effects and inhibition effects on peroxidation of linoleic acids. Total polyphenol and flavonoid contents were highest in Matricaria recutica. Scavenging activity on DPPH and ABTS radicals were highest with extracts of Echinacea angustifolia and Serratula coronata var. insularis f. insularis, respectively. Aerial palt extracts of all species showed lower DPPH scavenging activity than ascorbic acid and 2,6-Di-tert-butyl-4-methylphenol (BHT). But Serratula coronata var. insularis f. insularis demonstrated higher ABTS scavenging activity than ascorbic acid and BHT. In Hieracium pilosella, Echinacea angustifolia, Matricaria reculica extracts showed higher ABTS scavenging activity than BHT. Ferrous ion chelating effects was highest with Matricaria recutica extract, but the effects were much lower than ethylenediaminetetraacetic acid (EDTA). The inhibition activity in lipid peroxidation of linoleic acids was highest in Eupatorium japonicum with 90.06% inhibition 4 days after reaction and 40.52% after 24 days. This demonstrated higher inhibition activity and longer lasting than BHT. Aerial part of Matricaria recutica for extraction source, rather than flower, has higher potential for antiox.idant material. In conclusion, development of natural antioxidants in Compositae is possible by studying antioxidant activity of each species.

Nutritional Components and Antioxidant Activities of Commercial Loquat (Eryobotrya japonica) Leaf Tea (시판 비파나무잎차의 영양성분 및 항산화 활성)

  • Jeong, Chang-Ho;Kim, Il-Hun;Shim, Ki-Hwan;Bae, Young-Il
    • Journal of agriculture & life science
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    • v.45 no.4
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    • pp.105-112
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    • 2011
  • Nutritional components and antioxidant activities of commercial loquat leaf tea (CLLT) were evaluated. Proximate compositions were as follows; moisture 5.34%, crude protein 8.38%, crude fat 13.26%, nitrogen free extract 46.21%, crude fiber 19.24% and crude ash 7.57%, respectively. Ascorbic acid and total phenolics content of CLLT was 0.47 mg/100 g and 42.55 mg/GAE g, respectively. Mineral elements were Ca 1,624.01 mg/100 g, K 1,099.66 mg/100 g, and Mg 192.70 mg/100 g, respectively. Amino acid contents of CLLT were very rich in glutamic acid 565.98 mg/100 g and deficient in cystine 12.97 mg/100 g. The 2,2'-azino-bis (3-ethylbenzthiazoline-6- sulfonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of hot water extract from CLLT were 72.51% and 90.11%, respectively at a concentration of $1,000{\mu}g/mL$. Reducing power and ferric reducing antioxidant power (FRAP) of hot water extract from CLLT were increased in a dose dependent manner. Therefore, these results suggest that the hot water extract of CLLT possess antioxidant activities and thus it has great potential as a source for functional food such as natural antioxidant.