• Preventive Nutrition and Food Science
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• v.10 no.2
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• pp.111-117
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• 2005

Radical scavenging activity of water and methanol extracts of mulberry juice and cake prepared from mulberry fruit (Morus spp.) was evaluated using three in vitro assay systems. Mulberry fruits were homogenized with $0.5\%$ trifluoroacetic acid (TFA) in distilled water, filtered with cheeze-cloth and centrifuged to yield mulberry juice and cake. Mulberry juice was evaporated and solubilized in $0.5\%$ TFA in distilled water or $0.5\%$ TFA in $80\%$ aqueous methanol, followed by filtration and evaporation to obtain water (WMJ) and methanol (MMJ) extracts of mulberry juice. Mulberrry cake also was extracted with the above same solvents, and thereby finally obtaining water (WMC) and methanol (MMC) extracts of mulberry cake. Among four extracts, the MMC showed the most potent radical scavenging activity against DPPH radical $(IC_{50}=167.45\;{\mu}g/mL)$, and superoxide $(IC_{50}=36.18\;{\mu}g/mL)$ and hydroxyl radicals $(IC_{50}=467.08\;{\mu}g/mL)$. The WMC also exhibited stronger radical scavenging activity than those of two other mulberry juice extract, WMJ and MMJ. Meanwhile, the MMJ exerted stronger three radical scavenging activity than the WMJ. Total phenolic content of the water and MeOH extracts from mulberry cake was higher than that of the water and MeOH extracts from mulberry juice. Thus, these results suggest that the extracts of mulberry cake with high dietary phenolics may be useful potential source of natural antioxidant as radical scavenger.

• Development and Reproduction
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• v.14 no.3
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• pp.155-162
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• 2010

The trophectoderm is one of the earliest cell types to differentiate in the forming placenta. It is an important for the initial implantation and placentation during pregnancy. Trophoblast stem cells (TBSCs) develop from the blastocyst and are maintained by signals emanating from the inner cell mass. However, several limitations including rarity and difficulty in isolation of trophoblast stem cells derived from blastocyst still exist. To establish a model for trophoblast differentiation, we isolated TBSCs from human term placenta ($\geq$38 weeks) and characterized. Cell cycle was analyzed by measuring DNA content by FACS analysis and phenotype of TBSCs was characterized by RT-PCR and FACS analysis. TBSCs have expressed various markers such as self-renewal markers (Nanog, Sox2), three germ layer markers (hNF68, alpha-cardiac actin, hAFP), trophoblast specific markers (CDX-2, CK7, HLA-G), and TERT gene. In FACS analysis, TBSCs isolated from term placenta showed that the majority of cells expressed CD13, CD44, CD90, CD95, CD105, HLA-ABC, cytokeratin 7, and HLA-G. Testing for CD31, CD34, CD45, CD71, vimentin and HLA-DR were negative. TBSCs were shown to decrease the growth rate when cultured in conditioned medium without FGF4/heparin as well as the morphology was changed to a characteristic giant cell with a large cytoplasm and nucleus. In invasion assay, TBSCs isolated from term placenta showed invasion activities in in vivo using nude mice and in vitro Matrigel system. Taken together, these results support that an isolation potential of TBSCs from term placenta as well as a good source for understanding of the infertility mechanism.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.1
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• pp.70-78
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• 2009

Floodplain areas of major South Korean rivers were determined by analyzing topographical maps and hydraulic properties of floodplain soil were measured using disc tension infiltrometer. To assess the possibility of treating secondary effluents of municipal wastewater with floodplain soil, a computer code for the analysis of unsaturated flow in soil was employed along with searches conducted in the literature. Based on the data generated, an estimate of total floodplain filtration capacity in Korea was obtained. The results of our study reveal that Korean floodplains have surface soil that is adequate for treating water. Moreover, the distributions of floodplains are substantial over the entire reaches of the rivers, indicating that the conditions are favorable for floodplain filtration as additional treatment of secondary effluent. The capacity of floodplain filtration in Korea is circa 182,000,000 $m^3$/day and most of the rivers are estimated to have enough capacity of floodplain filtration to meet all the secondary effluent, indicating that this technology may be expected to make further improvements on river water quality. Furthermore, this method may also be applied to better the source-water quality for drinking water.

• Journal of Biosystems Engineering
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• v.38 no.1
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• pp.55-63
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• 2013

Purpose: The present study aimed at image processing methods for automatic cell pattern recognition and morphological analysis for tissue engineering applications. The primary aim was to ascertain the novel algorithm of adaptive brightness correction from microscopic images for use as a potential image analysis. Methods: General microscopic image of cells has a minor problem which the central area is brighter than edge-area because of the light source. This may affect serious problems to threshold process for cell-number counting or cell pattern recognition. In order to compensate the problem, we processed to find the central point of brightness and give less weight-value as the distance to centroid. Results: The results presented that microscopic images through the brightness correction were performed clearer than those without brightness compensation. And the classification of mixed cells was performed as well, which is expected to be completed with pattern recognition later. Beside each detection ratio of hBMSCs and HeLa cells was 95% and 92%, respectively. Conclusions: Using this novel algorithm of adaptive brightness correction could control the easier approach to cell pattern recognition and counting cell numbers.

• International Journal of Aeronautical and Space Sciences
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• v.18 no.3
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• pp.545-554
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• 2017

Among various applications of the High Altitude Long Endurance (HALE) Unmanned Aerial Vehicle (UAV), this paper has a focus on the Global Positioning System (GPS) utilizing pseudolite and its improved performance, particularly during the multi-purpose missions. In a multi-purpose mission, the HALE UAV follows a specified flight trajectory for both navigation applications and missions. Some of the representative HALE missions are remote exploration, surveillance, reconnaissance, and communication relay. During these operations, the HALE UAV can also be an additional positioning signal source as it broadcast signals using pseudolite. The pseudolite signal can improve the availability, accuracy, and reliability of the GPS particularly in areas with poor signal reception, such as shadowed regions between tall buildings. The improvement in performance of navigation is validated through simulations of multi-purpose missions of the solar-powered HALE UAV in an urban canyon. The simulation includes UAV trajectory generation at stratosphere and uses actual geographical building data. The results indicate that the pseudolite-equipped HALE UAV has the potential to enhance the performance of the satellite navigation system in navigationally degraded regions even during multi-purpose operations.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.7
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• pp.3327-3333
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• 2011

The interest on the recovery of thermal energy using the industrial waste has been rising to solve the problems of continuous increase of waste generation and the depletion of the fossil fuel recently. To recovery from the waste among the new recyclable energies has been proved as the most favorable when the potential value of energy source is compared. The RDF made from the industrial waste has been approved as the most economical method. This research has analyzed the heavy metal components containing in the industrial waste. The concentration of Cl and S in the industrial waste generated in C-industrial complex are slightly high than that of the B- and A-industrial complex. The main components generated from A-industrial complex, B-industrial complex, and C-industrial complex are alumina-silicates, calcium alumina silicates, and the mixture of lime and calcium alumina silicate. These results could be used to reveal the origin of inorganic components in industrial waste and utilized as a basic data to improve the performance of the RDF as fuel.

• Smart Media Journal
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• v.6 no.3
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• pp.41-48
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• 2017

Event detection using social media has been widespread since social network services have been an active communication channel for connecting with others, diffusing news message. Especially, the real-time characteristic of social media has created the opportunity for supporting for real-time applications/systems. Social network such as Twitter is the potential data source to explore useful information by mining messages posted by the user community. This paper proposed a novel system for temporal event detection by analyzing social data. As a result, this information can be used by first responders, decision makers, or news agents to gain insight of the situation. The proposed approach takes advantages of deep learning methods that play core techniques on the main tasks including informative data identifying from a noisy environment and temporal event detection. The former is the responsibility of Convolutional Neural Network model trained from labeled Twitter data. The latter is for event detection supported by Recurrent Neural Network module. We demonstrated our approach and experimental results on the case study of earthquake situations. Our system is more adaptive than other systems used traditional methods since deep learning enables to extract the features of data without spending lots of time constructing feature by hand. This benefit makes our approach adaptive to extend to a new context of practice. Moreover, the proposed system promised to respond to acceptable delay within several minutes that will helpful mean for supporting news channel agents or belief plan in case of disaster events.

• Resources Recycling
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• v.25 no.1
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• pp.31-39
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• 2016

Small household appliances such as electric rice cooker, a vacuum cleaner, an electric fan, etc. are diverse and complex due to the materials and components and waste streams from the manufacturing processes. In the present study, physical characterization of small e-wastes was analyzed on major items including electric rice cooker after manual dismantling. Small household appliances is an important potential source of waste plastic, however, recycling plastics from small e-waste is still unusual. The present communication gives results of separation processes on black plastics and the limitations of these sorting processes in used small household appliances.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2005.07a
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• pp.390-392
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• 2005

The digital radiation detectors are used clinically by diagnostic apparatus. However the digital radiation detector are some problem like high operating voltage, light blurring, low conversion efficiency, low fill factor, etc. Thus we propose a new radiation detector that the photoconductor layer and liquid crystal layer are coupled in sandwich structure. X-ray absorption in the photoconductor layer controls the state of the liquid crystal via creation of charge carrier and the light modulation of liquid crystal make image formation. The advantage of the new radiation detector is that high resolution image is acquired and the signal amplification is possible by external visible light source. In this study, we study the optical properties and electrical properties of the new radiation detector to irradiate X-ray. The Mercury Iodide($HgI_2$) was used by photoconductor material, and the aluminum is used by reflective layer. The thickness of Mercury Iodide is about $200{\mu}m$, the operating voltage of the liquid crystal is 1.5~5V. The electrical properties of Mercury Iodide was measured, and the transmission efficiency of liquid crystal was measured by modulation potential.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.167-171
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• 2010

The present study aims to evaluate Cordyceps militaris water extract (CMWE) with a view to develop melanogenesis inhibitors. Inhibitory activities of CMWE against tyrosinase, L-DOPA(L-3,4-dihydroxyphenylalanine) oxidation, and melanin biosynthesis in B16 mouse melanoma cells were investigated. CMWE, at $5000\;{\mu}g/ml$, inhibited tyrosinase activity of 71% and DOPA oxidation of 40% as reacting with L-DOPA. Furthermore, B16 mouse melanoma cell survived over 50% from low to high dose on MTT assay, and CMWE markedly inhibited (> 50%) melanin synthesis at $5000\;{\mu}g/ml$. The inhibitory effect of CMWE on melanogenesis was attributed to enhancement of tyrosinase degradation. Key enzyme of melanin biosynthesis is tyrosinase which catalyses a beginning step from tyrosine to DOPA quinine and melanin formation step, respectively. These results indicated that CMWE may be a potential source of novel whitening agents for cosmetic or therapeutic application.