The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Inhibitory Effects of Melanin Secretion on B16 Melanoma cell of Cordyceps militaris Water Extract

밀리타리스 동충하초 열수추출물의 멜라닌 분비 억제능 효과

  • Nam, Byung-Hyouk (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Jo, Wool-Soon (Dong Nam Institute of Radiological & Medical Sciences) ;
  • Choi, Yoo-Jin (Dong Nam Institute of Radiological & Medical Sciences) ;
  • Lee, Jae-Yun (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Kang, Eun-Young (Busan Techno-park Marine Bio-Industry Development Center) ;
  • Jeong, Min-Ho (Department of Microbiology, Dong-A University Medical College) ;
  • Lee, Jae-Dong (Department of Microbiology, College of Natural Sciences, Pusan National University)
  • 남병혁 (부산대학교 자연과학대학 미생물학과) ;
  • 조월순 (동남권원자력의학원) ;
  • 최유진 (동남권원자력의학원) ;
  • 이재윤 (부산대학교 자연과학대학 미생물학과) ;
  • 강은영 ((재)부산테크노파크 해양생물산업육성센터) ;
  • 정민호 (동아대학교 의과대학 미생물학교실) ;
  • 이재동 (부산대학교 자연과학대학 미생물학과)
  • Received : 2010.07.21
  • Accepted : 2010.11.17
  • Published : 2010.12.31
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Abstract

The present study aims to evaluate Cordyceps militaris water extract (CMWE) with a view to develop melanogenesis inhibitors. Inhibitory activities of CMWE against tyrosinase, L-DOPA(L-3,4-dihydroxyphenylalanine) oxidation, and melanin biosynthesis in B16 mouse melanoma cells were investigated. CMWE, at $5000\;{\mu}g/ml$, inhibited tyrosinase activity of 71% and DOPA oxidation of 40% as reacting with L-DOPA. Furthermore, B16 mouse melanoma cell survived over 50% from low to high dose on MTT assay, and CMWE markedly inhibited (> 50%) melanin synthesis at $5000\;{\mu}g/ml$. The inhibitory effect of CMWE on melanogenesis was attributed to enhancement of tyrosinase degradation. Key enzyme of melanin biosynthesis is tyrosinase which catalyses a beginning step from tyrosine to DOPA quinine and melanin formation step, respectively. These results indicated that CMWE may be a potential source of novel whitening agents for cosmetic or therapeutic application.

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References

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