• Microbiology and Biotechnology Letters
• /
• v.14 no.4
• /
• pp.311-318
• /
• 1986

Glucoamylase and ethanol productivities of HSDD-170 and HSDM-119 formed by S. cerevisiae and S. diastaticus protoplast fusion were investigated. For the production of the glucoamylase, soluble starch as carbon source, yeast extract and C. S. L as nitrogen source added into the basal medium were favorable. The production of the enzyme reached at maximum after cultivation of the fusant for 4 days at 3$0^{\circ}C$, aerobically. The properties of glucoamylase produced by fusants were very similar to those produced by S. diastaticus as based on optimum temperature, pH stability. In alcohol fermentation from starch, strain HSDD-170 fermented starch faster than either of its parental strains. The maximum of alcohol yield in 15% of liquefied potato starch was 7.5% (v/v).

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.66 no.4
• /
• pp.375-382
• /
• 2021

For stable cultivation in double cropping, it is important to use potato (Solanum tuberosum L.) cultivars with a short dormancy period of 50-70 days and plant seed tubers of appropriate type and size. An experiment was conducted during 2018-2019 to investigate the effects of seed tuber type and size on growth and yield performance in double cropping. Whole tubers of three sizes (10-20 g, 30-40 g, and 50-60 g) and conventional cut tubers weighing 30-40 g from three cultivars with different dormancy periods, namely 'Daeji' (40-60 days), 'Eunsun' (50-60 days), and 'Saebong' (50-80 days), were planted, and their field performance was compared. Regardless of the cultivar, the increase in the whole tuber weight up to 30-40 g led to fast emergence, thereby increasing ground cover rate, shoot growth rate, and tuber growth rate, which ultimately improved tuber yield by 33-54%. Comparing the whole and cut tubers, 'Daeji' and 'Eunsun' showed similar growth and yield performance; as such, the performance of whole tubers weighing 10-20 g was comparable to that of cut tubers weighing 30-40 g. However, 'Saebong', a cultivar with relatively long dormancy period, performed better with cut tuber than with whole tubers. Based on these results, we recommend the use of whole tubers weighing over 30 g for double cropping. Further studies to break tuber dormancy are warranted in cultivars with relatively long dormancy periods (50-80 days), such as 'Saebong'.

• Journal of Plant Biotechnology
• /
• v.35 no.1
• /
• pp.63-68
• /
• 2008

Single-node stem pieces ca. 1 cm in length containing a axillary bud were obtained from in vitro plants of potato (Solanum tuberosum L.). The influences by a position of the node and the existence of a leaf at the node were observed in the single-node culture on the 8% sucrose MS medium. The effect of CCC was also investigated for the microtuberization. The apical part node was excellent in the tuberization not to mention shoot length, fresh weight, diameter, the number of node on the in vitro culture of a single-node than the lower part. The differences in the diameter of a tuber formed in the part of the axillary bud on all treatments including the cultivation of the apical part node were not recognized. However, the fresh weight of the tuber showed high value in the tuber formed at the axillary bud of shoot apex part. At 20 days after cultivation, tuberization was promoted in the new stolen that developed from the bud of node with a leaf under SD condition of 8 hours at $20^{\circ}C$. The tuberization from axillary bud of the single-node without leaf was inhibited at high temperature of $28^{\circ}C$ regardless of daylength. Whereas, tuberization at $20^{\circ}C$ and $28^{\circ}C$ was similar without the difference under SD condition but the tuber formation ratio were low. CCC 500 mg/L promoted tuberization and the effect was also showed even under LD condition at $28^{\circ}C$. The inhibiton of tuberization under LD and high temperature condition could be solved by treatment with CCC.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.241-241
• /
• 2017

Sweet potatoes (Ipomoea batatas (L.) LAMK.,) have been cultivated in Central and South America for about 2000 years and are now grown mainly in Asia and South America. Sweet potatoes are annual in the temperate region, but are classified as perennial in the tropical region. In 2000, the cultivation area of sweet potatoes decreased to about 16,000 ha in 2000, but the cultivation area increased slightly in recent 20,000 ha in Korea. Sweet potatoes do not show higher maximum dry matter production of 120 ~ 150g per plant, and the leaf area index (LAI), which maximizes dry matter production, is known as 3.0 ~ 4.0. As the leaf area increase, the penetration of light into the canopy becomes poor, and sufficient photosynthesis cannot be achieved in the lower leaves, on the other hand the respiration increase, which results in poor dry matter production. This study was conducted to know the responses of sweet potatoes to intensive shading treatment of 80% shading. This experiment was conducted for about 42 days from September 6, 2016 to October 18, 2016 at Gyeongsang National University Experimental Farm, Jinju, Korea. The plant canopy was shaded with black nylon 80% shade cloth suspended 1.2 m above the ground. The photosynthetic rate, stomatal conductance, chlorophyll fluorescence, SPAD and NDVI were measured in 3 replicates every 7 days after shading initiation. After the fresh weight was measured, the samples were dried at $80^{\circ}C$ in a dry oven and measured. By the 80% shading treatment, chlorophyll fluorescence of the treated plants was slightly higher than that of the control, the SPAD value was higher by 3.4 and NDVI value was higher by 0.01. However, photosynthetic rate and stomatal conductance were lower than those of the control. The stomatal conductance of the control were two times higher than those of the control and the photosynthetic rate of the control was four times higher than that of the control. In control, plant showed a tendency to steadily increase in fresh weight and dry weight. However, in the case of shading treatment, the tendency to increase in the fresh and dry weight of tuberous roots was not clear. The fresh weight of shoot showed a tendency to increase steadily while the difference between treatment and control was not large, but tended to decrease after frost.

• The Korean Journal of Mycology
• /
• v.46 no.2
• /
• pp.177-185
• /
• 2018

The cultivation characteristics of 26 wild strains of Lentinula edodes were investigated for their use as breeding material. Strains NIFoS 68, 136, 1521, 1651, and 2064 showed an above average mycelial growth on potato dextrose agar at 10, 20, and $30^{\circ}C$. NIFoS 411 showed the lowest mycelial growth at $20^{\circ}C$, but the highest growth at $30^{\circ}C$. The rate of weight loss of L. edodes cultivated on sawdust (2 kg) ranged from 13.5 to 47.5%, with the highest rates showed by NIFoS 50 (47.5%), NIFoS 128 (34.5%), and NIFoS 54 (34.4%). Fruiting bodies were produced in nearly all (24/26) strains and productivity ranged from 3 g to 446 g/2 kg medium. Temperature was not significantly correlated with mushroom production or mycelial growth. Larger weight loss correlated strongly with fruit yield. In terms of production, NIFoS 50 (446 g), NIFoS 952 (435 g), and NIFoS 53 (421 g) were useful as breeding material. The NIFoS 667 strain was superior in terms of morphology. NIFoS 670 showed the characteristic yellowish-brown color of fruiting bodies.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.67 no.2
• /
• pp.121-130
• /
• 2022

This study was conducted to determine the suitable amount of irrigation and fertigation for the growth and yield of spring potatoes (Solanum tuberosum L.). To investigate the effect of soil moisture content on the growth and yield of potato, five irrigation treatments were performed based on soil moisture tension: -10 kPa, -20 kPa, -30 kPa, -40 kPa, and non-irrigation. The growth and yield among the irrigation treatments was the best in the -10 kPa treatment, where the yield increased by 47% compared to the non-irrigation treatment. To determine the standard of additional fertilizer for fertigation cultivation of spring potatoes, seven treatments were performed, including a control (basic fertilization without additional fertilizer), compound fertilizer (NPK) based on 0.5 times the standard amount of fertilizer (NPK 0.5; N-P₂O₅-K₂O: 2.5-2.2-3.25 kg/10a), NPK 0.8 (N-P₂O₅-K₂O: 4-3.5-5 kg/10a), and NPK 1.2 (N-P₂O₅-K₂O: 6-5.3-7.8 kg/10a), and potassium fertilizer of 2 kg (K 2), 5 kg (K 5), and 7 kg (K 7). The growth and yield among the fertilization treatments was highest in the K 5 treatment, where the yield increased by approximately 27% compared to the control. Therefore, this study suggests that irrigation with -10 kPa or fertigation with K 5 during the tuber initiation and tuber filling stage could increase the yield of spring potatoes.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.6
• /
• pp.1027-1034
• /
• 2011

In order to provide the basic information on the agricultural environment in Daegwallyeong Highland, the characters of weather, water, and soil quality were investigated. The meteorological characteristics was monitored by automatic weather system (AWS) at 17 sites. The quality of water for samples were collected monthly at 24 sites depending on landuse style. Soil samples were collected from a forest, grassland, and the major vegetable cultivation areas such as potato, carrot, Chinese cabbage, onion, head lettuce, and welsh onion field. The weather showed the mountain climate, and the average yearly temperature is $6.4^{\circ}C$, the average temperature in January is $-7.6^{\circ}C$ and the average temperature in July is $19.1^{\circ}C$, and the change of temperature on the districts of Daegwallyeong is severe. The yearly record of precipitation shows 1717.2 mm. The water quality of crop field was worse than forest or grassland in Daewallyeong highland. In 2005, annual T-N, T-P, SS distribution of Chinese cabbage field showed 7.4~11.3, 0.061~0.1, and $3.0{\sim}53.0mg\;L^{-1}$. The potato field showed 3.1~7.2, 0.019~0.056 and $0.5{\sim}3.0mg\;L^{-1}$, respectively. Being compared of water quality between potato field and chinese cabbage field, it showed that the water quality of Chinese cabbage field was worse than potato field. On farming, the soil of crop cultivation showed pH 5.6 to 6.8, $18.0{\sim}42.4g\;kg^{-1}$ of OM, $316{\sim}658mg\;kg^{-1}$ of Avail. $P_2O_5$. The content of cations showed $0.41{\sim}0.88cmol_c\;kg^{-1}$ of Exch. K, $3.73{\sim}7.07cmol_c\;kg^{-1}$ of Exch. Ca and $1.17{\sim}1.90cmol_c\;kg^{-1}$ of Exch. Mg.

• Journal of Ginseng Research
• /
• v.20 no.4
• /
• pp.472-500
• /
• 1996

Researches on mineral nutrition, physiology and phyrsiological diseases, . cultivaction methods. brceding. pest control quality management and extension during 1976-1995 in Korea were reviewed Review in brceding and pest control was restricted to the researches directely related to cultivaction. Mineral nulrient up take. partion and varicos factors such as top dreasing. Light intersity etc. and interrelationship between minerals were investigated. Top dressing was not effective due to low minera1 requorement Physiological characteristics on tempelature light and water were well elucidated and applied to assess traditional cultivation method and its inovation. Photosyrnthetic pigments. light harvest proteins and activity of related enzymes were studied. In nitrogen metabolism arginine, praline, ammonium, threonine appeared to have important role in re growth of shoot Saponin metabolism was studied in relation to growth and new ginsenosides were found but physiological role of saponin was not clearly elucidated yet Endogenous growth regulators were reported and various erogenous growth regulators were studied for growth stimulation. short stem and seed pruning etc. Various physiological diseases were investigated for cause and control measures were established. Water culture was little studied Forest culture was studied but not retched the recommendable stage Drip irrigation straw mulching. seasonal shading and soil preparation method including soil fertility adjustment were established for practical application. Shading materials completely changed to polyethylene net and materials of polymers The research on ginseng cultivation in paddy field opened the way to establish the permanent ginseng cultivation plantation Ginseng harvester and seeder were developed in the late 1950s. Transplanted and many other machines were developed in the early 1990s. In ginseng breeding only pure line selection was of practical significance several verities were at the stage of seed propagation at ginseng plantations. Mutation breeding (${\gamma}$-ray. X-ray chemicals) was not successful. The research on plantlet formation through tissue culture was a little progressed but still far behind to vegetative propagation. Disease control research was concentrated in the isolation and identification of pathogans. their ecological charactelistics and biological control and soil humigation. Potato root rot nematodes was found and control method was established. Insect and small animal control research was greatly progresses in identification, ecological investigation, and ecological and physical control. Weed control was less important due to the development of mulching method of ridge and ditch. Quality factors of raw ginseng in relation to red ginseng process were extensively studied. Traditional quality measures were elucidated in accordance with modern analytical chemistry resulting in the importance of peptides in the centrat part rather than ginsenosides For large root production growth promoting rootzone micrcorganisms (PGPRM) were isolated and active compounds were identified. Field test on PGPRM was on going. Varictus methods formality improvement through cultivation were developed. Management research of ginseng production was rare Extension was active throuch official and private organizations and through workshop for the extension specialists, and direct lectures to grower's. Extension services made the researcher to understand the existing problems at grower's fields. Research environment for ginseng production was in prime time only for three years when Korea Ginseng Research Institute was established then gradually aggravated.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.61 no.1
• /
• pp.70-77
• /
• 2016

The object of this study was to evaluate the effects of climatic elements on potato yield and create a model for estimating the potato yield. We used 35 yield data of Sumi variety produced in mulching cultivation from 17 regions over 11 years. According to the results, some climatic elements showed significant level of correlation coefficient with marketable yield of potato. Totally 22 items of climatic elements appeared to be significant. Especially precipitation for 20 days after planting (Prec_1 & 2), relative humidity during 11~20 days after planting (RH_2), precipitation for 20 days before harvest (Prec_9 & 10), sunshine hours during 50~41 days before harvest (SH_6) and 20 days before harvest (SH_9 & 10), and days of rain during 10 days before harvest (DR_10) were highly significant in quadratic regression analysis. 22 items of predicted yield ($Y_i=aX_i{^2}+bX_i+c$) were induced from the 22 items of climatic elements (step 1). The correlations between the predicted yields and marketable yield were stepwised using SPSS, statistical program, and we selected a model (step 2), in which 4 items of independent variables ($Y_i$) were used. Subsequently the $Y_i$ were replaced with the equation in step 1, $aX_i{^2}+bX_i+c$. Finally we derived the model to predict the marketable yield of potato as below. $$Y=-336{\times}DR_-10^2+854{\times}DR_-10-0.422{\times}Prec_-9^2+43.3{\times}Prec_-9\\-0.0414{\times}RH_-2^2+46.2{\times}RH_-2-0.0102{\times}Prec_-2^2-7.00{\times}Prec_-2-10039$$.

• Horticultural Science & Technology
• /
• v.29 no.3
• /
• pp.247-254
• /
• 2011

The agronomic characteristics, nutritional contents, and insect response of the potato clones transformed with a glufosinate ammonium resistance gene were evaluated. Among the 4 transgenic potato clones, the Bar 3 clone was selected as a promising one for commercialization. The Bar 3 clone showed similar tuber yield capacity but higher herbicide resistance as compared with the non-transgenic potato cv. Dejima. The herbicide resistance of the Bar 3 clone was more than 5 times higher when tested with the herbicide concentration recommended by the producer. The major agronomic characteristics of the Bar 3 clone were not different from those of the non-transgenic Dejima. The annual variation in yields and agronomic characteristics showed similar tendency for 2 years from the third to fourth generation after transformation. The tubers of the Bar 3 clone also showed low occurrence in common scab and physiological disorders such as cracking and secondary growth. But the reasons for such results are yet to be studied. Also, it was considered that the Bar 3 clone have a potential of reducing not only common scab occurrence but also soil erosion during potato cultivation in field. The nutritional contents (mineral compound, vitamin C and amino acid) and response to Spodoptera exigua of the transgenic potato clones were not significantly different.