• Journal of The Korean Society of Grassland and Forage Science
• /
• v.23 no.3
• /
• pp.159-168
• /
• 2003

This pot experiment was conducted in order to find out the effects of boron application($B_{0}$ /; control, $B_1$; 0.2, $B_2$; 2.0,$ B_3$; 6.0, $B_4$; 15.0 B me/pot) on the forage performance of pure and mixed cultures of orchardgrass and white clover. The 2nd part was concerned with the changes in the forage yields and concurrence index. The results obtained are summarized as follows: 1. The optimum boron application($B_2$) generally resulted in the increase of both forage yields, but the effects of boron application on them were different according to the forage species, whether it was a pure or mixed cultures, additional fertilization, and cutting order. The effects of boron application on the forage productivity were more obvious in white clover than in orchardgrass. 2. Owing to the decline of white clover as affected by the application of additional fertilizers, especially N, in the grass-clover mixed cultures, the effects of boron application on the white clover yields showed a numerical inferiority compared with the pure culture. It was recognized that the yield increase and botanical composition of white clover in grass-clover mixed cultures could be regulated by the application of additional fertilizers and boron. 3. The toxic boron application($B_3$ and $B_4$) resulted in a decreased yield of both forages. The yield change of orchardgrass tended to be similar between pure and mixed cultures, whereas it of white clover tended to be more negative in mixed than in pure cultures. 4. With the application of additional fertilizers, especially N, the botanical composition and concurrence index in grass-clover mixed cultures were relatively increased in orchardgrass, and decreased in white clover. The botanical composition of orchardgrass increased from 55% to 75%, whereas it of white clover decreased from 45% in the first half cutting to 25% in the second half cutting, respectively.

• Korean Journal of Soil Science and Fertilizer
• /
• v.17 no.3
• /
• pp.265-273
• /
• 1984

To investigate nitrogen supplying effect of some slowrelease N-fertilizers on barley in upland soil sulfurcoated urea(SCU), ureaform and melamine (1,3,5-Triazine-2,4,6-triamine) were treated and compared to urea. In addition, thiourea as a nitrification inhibitor was also tested. Effects of variable soil condition such as moisture content, pH and temperature on nitrogen supplying ability of the fertilizers and on growth of barley were studied through incubation test and pot culture and the obtained results were summarized as follows: The releasing rate of ammonia from urea, SCU, ureaform and melamine were resulted as 27-59%, 25-39%, 9-34% and 0.7-4.3% at maximum conversion rate, respectively. Nitrification rate of the tested fertilizers was higher at pH 6.54 markedly than at pH 4.73. Addition of thourea depressed the formation of $NO_3$ during four weeks of incubation period. Mixed application of ureaform with small amount of urea contributed to nitrogen supply till latter growth stage of barely Basal application of melamine showed lowest nitrogen supplying ability and injurious response on barley growth.

• The Korean Journal of Food And Nutrition
• /
• v.26 no.4
• /
• pp.753-758
• /
• 2013

This study evaluated the quality characteristics for optimization of premix-type Buckwheat (Fagopyrum esculentum) Soksungjang (BS). First, according to the amount of salt (8, 10, 12%), coliform counts were not detected in BS with 10 or, 12% salt at 21 days and Bacillus subtilis HJ 18-4 as a starter at 7 days. Therefore, 10% salt with starter culture might be considered as suitable conditions for safety. Second, according to the ratio of water (1:1, 1:1.5, 1:1.8), BS prepared with the same weights of meju powder and water showed the highest aminotype nitrogen content (688.76mg%) at 35 days; thus, an adequate fermentation state was established at this condition compared to others. Third, according to the type of container (pot, plastic, glass), the aminotype nitrogen contents were higher in the plastic container and pot than in the glass bottle. Lastly, according to the batch size (1.25, 2.5 kg), the coliform of the 2.5 kg sample decreased faster than that of 1.25 kg samples at 14 days. These results suggest that BS prepared with the same ratio of water and meju powder fermented with HJ 18-4 as a starter and 10% salt in a pot, plastic container (3 weeks) and glass container (4 weeks) showed desirable fermentation qualities.

• Korean Journal of Medicinal Crop Science
• /
• v.7 no.4
• /
• pp.282-287
• /
• 1999

This study was conducted to investigate the effects of different soil texture in agronomic characteristics, root yield and quality of baloon flower (Platycodon grandiflorum A. DC.). Three kinds of soil texture, sandy loam, silt loam and clay loam, were tested for two years. Baloon flowers were seeded with 20cm row space and cultivated in the one square meter pot with 0. 6m depth. Fifty plants per pot were remained. In growth characteristics of one-year-old plants, shooting date was not significantly different according to the changes of soil texture. The ratio of stem growth was highest between July and August. In two-years-old plants, it was showed between May and June. Plants grown on clay loam showed the highest growing characteristics than those of other soil textures. Dry root weight cultivated on clay loam was 274kg/10a in one-year-old and 934kg/10a in two-years-old plants. The content of ethanol extracts from root was highest at those of sandy loam as 47.1 % in one-year-old root. The crude saponin content was not different significantly among the three kinds of soil texture.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.40 no.5
• /
• pp.619-628
• /
• 1995

Crude aqueous extracts from dried leaves, stems, roots, and flowers from both field grown and greenhouse grown alfalfa plants inhibited alfalfa seed germination and seedling growth. The degree of inhibition was greater in the field grown plant extracts. Flowers extract of field grown plant most inhibited alfalfa germination and seedling growth. In the concentration study, the highest concentration of extract (9.0%, w/v) significantly inhibited total alfalfa seed germination by 50% as compared to control. In partitioning study using pot hydroponic culture of plant biomass into leaves, stems, root, LAR products of LWR and SLA exhibited significant variation among four species. This result support that the inhibitory effect of autotoxic substances presenting in alfalfa tissue may be possible interference with the patitioning of biomass into leaf component relative to the total biomass produced by the alfalfa plant. Toxicity of extract was not reduced by adding activated charcoal, Dowex-50W, amberlite to the extract. Toxic substances existing in most plant tissues but mainly above ground foliage are water soluble and stable and may persist in old alfalfa fields. Thus, it is recommended to remove as much as possible of the above growth parts, especially vegetative stage, before one tries to re-establish alfalfa in former field of alfalfa.

• FLOWER RESEARCH JOURNAL
• /
• v.16 no.3
• /
• pp.205-210
• /
• 2008

Sedum kamtschaticum has been used as one of the native valuable plants for ornamental purpose. This experiment was conducted to find out the optimum media for pot cultured Sedum kamtschaticum using peatmoss and coir-based mixtures. Four mixtures such as peatmoss:perlite(5:5, v/v), peatmoss:perlite(7:3, v/v), coir:perlite(5:5, v/v), and coir:perlite(7:3, v/v) were used. Plants were irrigated with a Sonneveld solution in an ebb & flow system. The content of T-N, K, and Ca in the peatmoss-based media was higher than those of the coir-based media. The concentration of T-N, K, and Ca in the shoots of Sedum kamtschaticum increased when plants were cultured with peatmoss-based media. In the peatmoss-based media, plant growth, such as number of branches, leaf length and shoot fresh and dry weights, were significantly increased than those of plant grown in the coir-based media. As the results, the optimum media for pot cultured Sedum kamtschaticum in an ebb & flow system was considered to be the peatmoss-based mixtures.

• Korean Journal of Soil Science and Fertilizer
• /
• v.19 no.2
• /
• pp.147-151
• /
• 1986

A study was carried out to investigate yield, yield components, and change of chemical components in rice plants grown under different concentration of lead compound in nutrient solution of sandy culture. The results obtained are as follows. 1. Increasing lead concentration in culture solutions, pb content in the plant was increased and crop yields were decreased. 2. The critical pb concentration at which yield was declined, was 13.6 ppm. 3. As adding higher concentration of lead in nutrient solutions, concentration of lead in rice plant was significantly increased on leaf blade, and brown rice in pb. 4. Yield loss was closely related to pb content in leaf blade.

• The Korean Journal of Mycology
• /
• v.22 no.2
• /
• pp.184-189
• /
• 1994

The possibility of solid-substrate fermentation of Coriolus versicolor for the production of protein-bound polysaccharides(PBP) was studied. Zeolite and orchid-pot soil were used as solid materials for the culture because of the desirable physical properties. Glucose, sucrose and starch showed to be good carbon sources for the production of PBP by the solid-substrate fermantation of C. versicolor. Among the nitrogen sources, bactosoyton and peptone were very effective for the PBP production. The optimum pH for solid-substrate culture for the production of PBP was at the range of 5-6. The yields of PBP reached to 5-6 mg per 100 g solid-substrate.

• Korean Journal of Soil Science and Fertilizer
• /
• v.49 no.4
• /
• pp.401-408
• /
• 2016

The present study described the isolation of 2-methylbutyric acid (2-MBA) produced from Bacillus pumilus L1, to subsequently investigate its nematicidal activity for the control of the root-knot nematode. The results showed that 2-MBA could be purified by chromatographic techniques and was identified using nuclear magnetic resonance and liquid chromatography-mass spectrometry. Crude extract and partially purified compounds had a significant effect on the inhibition of egg hatchability and second-stage juvenile (J2) mortality. A dose-dependent effect of 2-MBA was observed for J2 mortality and egg hatchability. Egg hatchability was 69.2%, 59.9%, 32.7%, and 0.0% at 125, 250, 500, and $1000{\mu}g\;mL^{-1}$ of 2-MBA after 4 d of incubation, respectively. Meanwhile, J2 mortality was in the range of 24.4%-100.0% after 2 d of incubation, depending on the concentrations of 2-MBA used. A pot experiment also demonstrated that treatment of B. pumilus L1 culture caused a significant reduction in the number of galls, egg masses, and J2 population than that of the tap water (TW) control. However, as the B. pumilus L1 culture concentration was decreased, the efficacy of nematode control by treatment of B. pumilus L1 culture was reduced compared to that of TW. B. pumilus L1 inoculation at different concentrations also promoted cucumber plant growth. Therefore, our study demonstrated the potential of 2-MBA from B. pumilus L1 as a biocontrol agent against the root-knot nematode and a plant growth promoter for cucumber plants.

• Journal of Forest and Environmental Science
• /
• v.33 no.3
• /
• pp.197-201
• /
• 2017

Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.