• Environmental Engineering Research
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• 제19권1호
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• pp.75-81
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• 2014

Methanotrophic denitrification under a non-aerated condition (without external supply of oxygen or air) was investigated in a bioreactor coupled with a membrane diffuser. Batch experiment demonstrated that both methane consumption and nitrogen production rates were not high in the absence of oxygen, but most of the nitrate was reduced into $N_2$ with 88% recovery efficiency. The methane utilized for nitrate reduction was determined at 1.63 mmol $CH_4$/mmol $NO_3{^-}$-N, which was 2.6 times higher than the theoretical value. In spite of no oxygen supply, methanotrophic denitrification was well performed in the bioreactor, due to enhanced mass transfer of the methane by the membrane diffuser and utilization of oxygen remaining in the influent. The denitrification efficiency and specific denitrification rate were 47% and 1.69 mg $NO_3{^-}-N/g\;VSS{\cdot}hr$, respectively, which were slightly lower than for methanotrophic denitrification under an aerobic condition. The average concentration of total organic carbon in the effluent was as low as 2.45 mg/L, which indicates that it can be applicable as a post-denitrification method for the reclamation of secondary wastewater effluent. The dominant fatty acid methyl ester of mixed culture in the bioreactor was $C_{16:1{\omega}7c}$ and $C_{18:1{\omega}7c}$, which was predominantly found in type I and II methanotrophs, respectively. This study presents the potential of methanotrophic denitrification without externally excess oxygen supply as a post-denitrification option for various water treatment or reclamation.

• 한국물환경학회지
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• 제23권5호
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• pp.642-649
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• 2007

The effectiveness of add-on tertiary treatment processes for the polishing of the effluent of a biological nutrient removal (BNR) system from a modified $A^2/O$ process has been examined under the field condition with pilot-scale plants. The add-on treatment processes of 1) combined biofilm anoxic reactor and sand filtration, and 2) two-stage denitrification filter had been operated with various operating conditions. The experimental results indicated that two-stage denitrification filter could produced a better polished tertiary effluent. Filtration rate of $150m^3/m^2{\cdot}d$ for the 2-stage denitrifying filter could decrease the nitrate removal probably due to shorter detention time that caused insufficient reaction for denitrification. Two stage denitrification filter operated with M/N ratio of 3.0 and filtration rate of $100m^3/m^2{\cdot}d$ produced the tertiary effluent with nitrate and SS concentraitons of 2.8 mg/L and 2.3 mg/L, respectively. When the operating temperature reduced $30^{\circ}C$ to $18^{\circ}C$, $NO_3{^-}-N$ removal efficiency decreased from 73% to 68%.

• Journal of Microbiology and Biotechnology
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• 제18권11호
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• pp.1826-1835
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• 2008

An autotrophic denitrification reactor (ADR-l) and a heterotrophic denitrification reactor (HDR-2) were operated to remove nitrate and nitrite in an anoxic environment in raw sewage. The $NO_3$-N removal rate of ADR-l was shown to range from 52.8% to 78.7%, which was higher than the $NO_3$-N removal rate of HDR-2. Specific denitrification rates (SDNR) of ADR-l and HDR-2 were 3.0 to 4.0 and 1.1 to $1.2\;mgNO_3$-N/gVSS/h, respectively. From results of restriction fragment length polymorphism (RFLP) of the 16S rRNA gene, Aquaspirillum metamorphum, Alcaligenes defragrans, and Azoarcus sp. were $\beta$-Proteobacteria that are affiliated with denitritying bacteria in the ADR-l. Specifically, Thiobacillus denitrificans was detected as an autotrophic denitrification bacteria. In HDR-2, the $\beta$-Proteobacteria such as Denitritying-Fe-oxidizing bacteria, Alcaligenes defragrans, Acidovorax sp., Azoarcus denitrificans, and Aquaspirillum metamorphum were the main bacteria related to denitrifying bacteria. The $\beta$-and $\alpha$-Proteobacteria were the important bacterial groups in ADR-l, whereas the $\beta$-Proteobacteria were the main bacterial group in HDR-2 based on results of fluorescent in situ hybridization (FISH). The number of Thiobacillus denitrificans increased in ADR-l during the operation period but not in HRD-2. Overall, the data presented here demonstrate that many heterotrophic denitritying bacteria coexisted with autotrophic denitrifying bacteria such as Thiobacillus denitrificans for nitrate removal in ADR-l. On the other hand, only heterotrophic denitritying bacteria were identified as dominant bacterial groups in HDR-2. Our research may provide a foundation for the complete nitrate removal in raw sewage of low-COD concentration under anoxic condition without any external organic carbon or the requirement of post-treatment.

• 대한환경공학회지
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• 제35권6호
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• pp.407-414
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• 2013

가축분뇨 공공처리시설 및 개별농가에서도 적용이 가능하도록 경제적이고 운영이 비교적 쉽고 간단하며 부지가 적게 소요되는 컴팩트한 처리시설을 개발하기 위하여 안성에 있는 M농가에 실규모의 처리시설(100톤/일)을 설치하고 공공처리시설 방류수 수질기준까지 처리할 수 있도록 운영하였다. 벨트프레스 탈수기를 통해 고액분리된 액상 가축분뇨는 MBR/NF/RO를 통해 처리되고 NF/RO농축수 및 가축분뇨처리 슬러지는 혼합한 뒤 탈질을 거쳐 응집가압부상을 통해 처리된다. MBR/NF/RO처리수와 가압부상조 유출수를 혼합 방류하면 공공처리장 수질기준 BOD 30 mg/L, T-N 60 mg/L, T-P 8 mg/L 이하가 달성된다. 가압부상 농축 슬러지는 벨트프레스 탈수기로 유입되어 고상 가축분뇨와 함께 탈수되어 분리된 고형물의 수분함량은 90%로 톱밥과 혼합되어 퇴비로 활용된다.

• 환경위생공학
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• 제15권3호
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• pp.15-23
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• 2000

This study's purpose is the investigating the created liquid post-acid fermentation of usability of denitrification as exterior carbon sources by loading capacity of Organic matters. The time of acid fermentation of food waste, the slower loading capacity of organic matter, the faster decomposition fate, but the density of generation $VFA_S$ was weak and, $SBOD_5$ : ST-N rate and $SBOD_5/SCOD_{Cr}$ rate was low. Between TS and VS, VS was decreased to 6th day rast, and then was decreased slowly. Two days after stating the experiments, $SCOD_{Cr}$, $SBOD_5$, STOC and $VFA_S$ was decreased or increased slowly, and then increased fast. And after showing the highest density, it was tended to decreased fast. About 5 days after, because about 5 days later $CH_4$ bacteria activity due to the accumulation of $VFA_S$ was limited, $CH_4$ density was weak. When highest density in the acquisition of VFA, $R_1$ was $10,120mg/{\ell}$, $R_2$ was $11,380mg/{\ell}$, $R_3$was $13,720mg/{\ell}$. So $R_3$ only cut was highest generation. At the time of $SBOD_5$ with the highest density, at $SBOD_5$ : ST-N ratio, $R_1$ was 243:1, $R_2$ was 278:1, $R_3$ was 293:1. All of these were high $SBOD_5$ : ST-N ratios. And $R_3$ was the highest. After studying $SBOD_5$ : ST-N ratio and $SBCD_5/SCOD_{Cr}$ ratio, as a carbon source of biological denitrification it was profitable composition ratio.

• 환경위생공학
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• 제16권2호
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• pp.32-37
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• 2001

The purpose of this study was to investigate the created liquid post-acid fermentation of usability of denitrification as exterior carbon sources by pH control. The time of acid fermentation of food waste, the slower loading capacity of organic matter, the faster decomposition rate, but the density of generation Volatile Fermentation Acids(VFAs), was weak and, $SBOD_{5}$ : ST-N rate and $SBOD_{5}/SCOD_{Cr}$ rate was low. Between TS and VS, VS was decreased to 4.5th day fast, and then was decreased slowly. 1.5 days after stating the experiments, $SCOD_{Cr}$, $SBOD_{5}$, STOC and $VFA_{s}$ was decreased of increased slowly, and then increased fast. And after showing the highest density, it was tended to decreased fast. At the time of $SBOD_{5}$ with the highest density, at $SBOD_{5}$ : ST-N ratio, $R_1$ was 303:1, $R_2$ was 319:1, $R_3$was the highest. After studying $SBOD_{5}$ : ST-N ratio and $SBOD_{5}/SCOD_{Cr}$ ratio, as a carbon source of biological denitrification it was profitable composition ratio.

• 한국물환경학회지
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• 제23권5호
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• pp.776-780
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• 2007

A biofilm reactor packed with porous media was investigated for nitrogen removal of synthetic wastewater. The effect of different loading rates on the nitrification was sustained to be steady state with stable efficiency of 50~60% in the range of $0.0083{\sim}0.017gNH_4-N/gMLVSS{\cdot}day$ of F/MN ratio and $1{\sim}2kgNH_4-N/m^3{\cdot}day$ of media volumetric loading rate. However, nitrification efficiency was rapidly decreased to 25~30% as F/MN ratio and media volumetric loading rate were increased to the range of $0.025{\sim}0.034gNH_4-N/gMLVSS{\cdot}day$ and $3{\sim}4kgNH_4-N/m^3{\cdot}day$, respectively. Also the consumption rate of alkalinity was higher under 8 hours of HRT than unter 6 hours of HRT. Accordingly the influent loading rate variation by detention time with influent flow influenced more on the nitrification efficiency than the influent loading rate variation by the influent concentration did. The temperature effect on the nitrification showed 25% higher in summer than in winter as the results reported by other researchers who reported that the nitrification efficiency in biofilm showed 20% increase from 55% to 75% when the temperature was raised from $20^{\circ}C$ to $25^{\circ}C$. Denitrification with sulfur-media showed 90% removal efficiency under steady-state with no effect from the increase of influent concentration and empty bed contact time (EBCT) change such as EBCT was decreased from 8.4 hr to 4.3 hr and $NO_3-N$ loading rate was changed within the range of $0.1{\sim}0.4kgNO^3-N/m^3{\cdot}day$. Accordingly Denitrification with sulfur-media is feasible for post denitrification at the concentration less than $80mgNO^3-N/L$.

• 한국지반환경공학회 논문집
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• 제4권3호
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• pp.61-67
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• 2003

축산폐수를 처리 시 우선 탈수 후 고상은 퇴비화, 액상을 연속회분식반응기(Sequencing batch reactor, SBR)로 이용하여 처리하는 시스템을 구상하였다. 영양염류 제거를 위한 SBR 공정의 안정적인 운전을 위한 운전모드 결정 실험을 수행하였다. K시 공공축산폐수처리장의 원심분리기에서 나온 유출수를 사용한 실험에서 질소를 제거하기 위한 적정 fill ratio는 1/12, SRT는 15일, 폭기/비폭기 주기는 2시간/1시간이었다. 탈질을 위하여 주입한 외부탄소원으로는 메탄올을 사용하였고 single feeding 방법과 step feeding 방법을 사용하였다. 이 결과 step feeding 방법을 사용시 더 효과적으로 유기물을 사용 탈질효율을 증가시킬 수 있었다.

• 한국환경과학회지
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• 제14권2호
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• pp.157-165
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• 2005

This study was conducted to determine optimum design parameters in nitrification and denitrfication of chemical fertilizer wastewater using pilot plant, Jet Loop Reactor. The chemical fertilizer wastewater which contains low amounts of organic carbon and has a high nitrogen concentration requires a post-denitrfication system. Organic nitrogen is hydrolyzed above $86\%$, and the concentration of organic nitrogen was influent wastewater 126mg/L and of effluent wastewater 16.4mg/L, respectively. The nitrification above $90\%$ was acquired to TKN volumetric loading below $0.5\;kgTKN/m^3{\cdot}d$, TKN sludge loading below $0.1\;kgTKN/kgVSS{\cdot}d$ and SRT over 8days. The nitrification efficiency was $90\%$ or more and the maximum specific nitrification rate was $184.8\;mgTKN/L{\cdot}hr$. The denitrification rate was above $95\%$ and the concentration of $NO_3-N$ was below 20mg/L. This case was required to $3\;kgCH_3OH/kgNO_3-N$, and the effluent concentration of $NO_3^--N$ was below 20mg/L at $NO_3^--N$ volumetric loading below $0.7\;kgNO_3^--N/m^3{\cdot}d$ and v sludge loading below $0.12\;kgNO_3^-N/kgVSS{\cdot}d$. At this case, the maximum sludge production was $0.83\;kgTS/kgT-N_{re}$ and the specific denitrfication rate was $5.5\;mgNO_3-N/gVSS{\cdot}h$.

• 한국입자에어로졸학회지
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• 제15권1호
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• pp.1-13
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• 2019

Current desulfurization and denitrification technologies have reached a considerable level in terms of reduction efficiency. However, when compared with the simultaneous reduction technology, the individual reduction technologies have issues such as economic disadvantages due to the difficulty to scale-up apparatus, secondary pollution from wastewater/waste during the treatment process, requirement of large facilities for post-treatment, and increased installation costs. Therefore, it is necessary to enable practical application of simultaneous SOx and NOx treatment technologies to remove two or more contaminants in one process. The present study analyzes a technology capable of maintaining simultaneous treatment of SOx and NOx even at low temperatures due to the electrochemically generated strong oxidation of the wet-pulse complex system. This system also reduces unreacted residual gas and secondary products through the wet scrubbing process. It addresses common problems of the existing fuel gas treatment methods such as SDR, SCR, and activated carbon adsorption (i.e., low treatment efficiency, expensive maintenance cost, large installation area, and energy loss). Experiments were performed with varying variables such as pulse voltage, reaction temperature, chemicals and additives ratios, liquid/gas ratio, structure of the aeration cleaning nozzle, and gas inlet concentration. The performance of individual and complex processes using the wet-pulse discharge reaction were analyzed and compared.