• 한국간호교육학회지
• /
• 제6권1호
• /
• pp.7-22
• /
• 2000

The purpose of this study was to identified the level of the exposure to the PC Pornography and what's their post-exposure reactions to the PC pornography. The subjects were 423 middle school boys in Pusan. The survey was executed from July 12 through October 30, 1999. The collected data were analyzed by SPSS WIN using the %, mean, ANOVA. The results of this study were as follows: 1. 79.4%(336 boys) were exposed to the PC pornography. 46.3%(196 boys) answered that they experienced the published pornography prior to the PC pornography. 2. 78.5%(333 boys) answered that they had their PC in their homes. This ratio revealed the higher level of possession. 67.6% said that their PC were set in their own rooms. 57.9% of the subject answered they experienced the Internet Communications. 68.6% of the subject said that they tried PC communications once or twice in a week. 38.5% of the subject expressed that they used their own PC for 1-2 hours in a day. Most of them(82.7%) enjoyed the PC games or entertainment programs. 81.3% of the subject answered that their school performance didn't show the progress after the use of their own PC. 78.3% of the subject answered that they acquired information. 45.2% of the subject answered that they made their friend through the Internet communications. 78.7% of the subject answered that the time of family dialogue decreased. 70.7% of the subject answered that the time of their TV watching decreased. 3. As for their post-exposure reactions the subject who were exposed to the PC Pornography showed the point 2.93 in the item 'pleasure'. The point of 'sexual impulses' was 2.81. But the point of 'anxiety' was 2.75, the point of 'dislike or disgust' was 2.62, the point of 'guilty' was 2.59. 4. According to the frequency, the motivation and the companionship on the exposure to PC Pornography, their post-exposure reaction scores showed differences significantly. Finally, the post-exposure reaction had relation to the frequency, the motivation, the companionship and the place (F=3.28 ~ 8.40, p=.012 ~ .000).

• Nuclear Engineering and Technology
• /
• 제55권10호
• /
• pp.3535-3542
• /
• 2023

Eutectic reactions of five kinds of Cr-coated Zr alloy cladding with different base materials (Zr-Nb-Sn alloy or Zr-Nb alloy), different coating thicknesses (6~22.5 mm), and different coating materials (Cr single layer or Cr/CrN bilayer) were studied using Differential Scanning Calorimetry (DSC). The DSC experiments demonstrated that the onset temperatures of the Cr single layer coated specimens were almost identical to ~1308 ℃, regardless of base materials or coating thicknesses. This study demonstrated that the Cr/CrN bilayer coated Zr-Nb-Sn alloy has a slightly (~10 ℃) higher eutectic onset temperature compared to the single Cr-coated specimen. The eutectic region characterized by post-eutectic microstructure proportionally increases with coating thickness. The post-eutectic characterization with different holding times at high temperature (1310-1330 ℃) reveals that progression of Zr-Cr eutectic requires time, and it dramatically changed with exposure time and temperature. The practical value of the time gain in non-instantaneous eutectic formation in terms of safety margin, however, seems to be limited.

• 인간식물환경학회지
• /
• 제21권6호
• /
• pp.557-564
• /
• 2018

Along with the aging society, the prevalence of dementia is also increasing. Dementia causes short-term memory loss as well as difficulties of performing daily activities and gradually causes suffering of the patients and their family. In spite of various programs for prevention of dementia of older people are being implemented, there is a lack of developing natural-based program for physical and mental health promotion. Therefore, it is necessary to develop programs for the elderly living alone who are more vulnerable to dementia because of their social and economic isolation. The purpose of this study was to develop a natural-based program and investigate the effects of 10 weeks forest therapy program for dementia prevention to improve the psychological and physical health of the elderly living alone. The experimental subjects were 30 elderly (aged 65 or older) and 31 elderly participated in control group. The Stress response, depressive symptoms, weight, body mass index (BMI), fat mass and muscle mass were measured for pre and post test. The results showed that the experimental group showed subjective stress relief (t=5.249, p=.000), improvement in symptoms of depression (t=4.152, p=.000), and decreases in weight (t=2.686, p=.012), BMI (t=2.629, p=.014) and fat mass (t=2.918, p=.007) after the forest therapy program. The experimental group showed lower stress reactions(t=-7.185, p=.000) and less depressive symptoms (t=-5.303, p=.000) than control group after participating the program. These results suggest that periodic forest exposure can help having less stressful and depressive status than non-forest exposure and the forest therapy program can reduce participants' psychological and physical risk factors of dementia.

• Molecules and Cells
• /
• 제43권10호
• /
• pp.848-855
• /
• 2020

Cells assemble stress granules (SGs) to protect their RNAs from exposure to harmful chemical reactions induced by environmental stress. These SGs release RNAs, which resume translation once the stress is relieved. During stem cell differentiation, gene expression is altered to allow cells to adopt various functional and morphological features necessary to differentiate. This process induces stress within a cell, and cells that cannot overcome this stress die. Here, we investigated the role of SGs in the progression of stem cell differentiation. SGs aggregated during the neuronal differentiation of human bone marrow-mesenchymal stem cells, and not in cell lines that could not undergo differentiation. SGs were observed between one and three hours post-induction; RNA translation was restrained at the same time. Immediately after disassembly of SGs, the expression of the neuronal marker neurofilament-M (NF-M) gradually increased. Assembled SGs that persisted in cells were exposed to salubrinal, which inhibited the dephosphorylation of eukaryotic translation initiation factor 2 subunit 1 (eIF2α), and in eIF2α/S51D mutant cells. When eIF2α/S51A mutant cells differentiated, SGs were not assembled. In all experiments, the disruption of SGs was accompanied by delayed NF-M expression and the number of neuronally differentiated cells was decreased. Decreased differentiation was accompanied by decreased cell viability, indicating the necessity of SGs for preventing cell death during neuronal differentiation. Collectively, these results demonstrate the essential role of SGs during the neuronal differentiation of stem cells.

• Journal of Microbiology and Biotechnology
• /
• 제29권4호
• /
• pp.651-657
• /
• 2019

Although smallpox was eradicated in 1980, it is still considered a potential agent of biowarfare and bioterrorism. Smallpox has the potential for high mortality rates along with a major public health impact, eventually causing public panic and social disruption. Passive administration of neutralizing monoclonal antibodies (mAbs) is an effective intervention for various adverse reactions caused by vaccination and the unpredictable nature of emerging and bioterrorist-related infections. Currently, vaccinia immune globulin (VIG) is manufactured from vaccinia vaccine-boosted plasma; however, this production method is not ideal because of its limited availability, low specific activity, and risk of contamination with blood-borne infectious agents. To overcome the limitations of VIG production from human plasma, we isolated two human single-chain variable fragments (scFvs), (SC34 and SC212), bound to vaccinia virus (VACV), from a scFv phage library constructed from the B cells of VACV vaccine-boosted volunteers. The scFvs were converted to human IgG1 (VC34 and VC212). These two anti-VACV mAbs were produced in Chinese Hamster Ovary (CHO) DG44 cells. The binding affinities of VC34 and VC212 were estimated by competition ELISA to $IC_{50}$ values of $2{\mu}g/ml$ (13.33 nM) and $22{\mu}g/ml$ (146.67 nM), respectively. Only the VC212 mAb was proven to neutralize the VACV, as evidenced by the plaque reduction neutralization test (PRNT) result with a $PRNT_{50}$ of ~0.16 mg/ml (${\sim}1.07{\mu}M$). This VC212 could serve as a valuable starting material for further development of VACV-neutralizing human immunoglobulin for a prophylactic measure against post-vaccination complications and for post-exposure treatment against smallpox.