• Nuclear Engineering and Technology
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• v.15 no.3
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• pp.179-187
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• 1983

The pH of containment spray and sump water following a LOCA for KNU 5'||'&'||'6 and KNU 1 was calculated to see if pH design criteria of containment spray system established by USNRC were met. The pH calculations have been made for the two cases; maximum pH and minimum pH. For KNU 5'||'&'||'6, results showed that long term sump pH values calculated for the maximum pH and minimum pH case well met the pH requirement of at least 8.5 and spray pH for the maximum case slightly exceeded the range of design criteria (8.5 to 11.0). For KNU 1, pH requirement of long term sump pH was also met, however, spray pH value for the maximum pH case was very largely greater than that of current pH requirement. (No pH requirement of containment spray water has been established at the time of designing KNU 1) In order to find the design parameters of containment spray system which are expected to meet the spray pH requirement, several calculations were wade, by changing the input parameters to "LCCAPH". Finally, it was shown that the boric acid concentration in RWST (refueling water storage tank), which was the primary sources of containment spray water during injection mode, be maintained the range of 2750 ppm to 2850 ppm, or tile flow rate of NaOH added to spray water he kept between 10 gpm to 24 gpm.

• Journal of Digital Convergence
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• v.14 no.11
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• pp.441-448
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• 2016

The purpose of this study was to identify the effects of mentoring senior core fundamental nursing skills education on clinical competence and critical thinking disposition in nursing students. The research design was a one group pre-posttest design and the participants were 57 nursing students. The contents consisted of 3 core fundamental nursing skills of vital sign, nelaton catheterization and subcutaneous injection and total duration of 6 hours. Mentoring seniors conducted to rapport forming & education and evaluated them after exercise and evaluation under the guidance of professor. Data were collected using self-administered questionnaires in pre-test and post-test, the collected data were analyzed using paired t-tests. The result shows the scores of clinical competence and critical thinking disposition were increased significantly after mentoring senior core fundamental nursing skills education in nursing students. The results indicate that this study was effective in improving nursing students clinical competence and critical thinking disposition in core fundamental nursing skills and is expected to be utilized in nusing practice education. Additional studies including those on control groups are recommended to compare difference between the experimental group and control group.

• Journal of Veterinary Clinics
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• v.28 no.3
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• pp.323-327
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• 2011

A Jindo dog (8-month-old, intact male) was referred for hind limb lameness on the right side. The dog was diagnosed with a simple femoral fracture by radiological examination. After surgical fixation of the femoral fracture, tramadol: a narcotic-like synthetic analgesic was intravenously administrated for post-operative analgesia. After injection of the tramadol, generalized tonic clonic seizure was immediately occurred in the dog. Seventeen hours later, the dog died despite intensive care. We suspected that tramadol might induce the seizurogenic effect resulted in death. A necropsy was performed to examine the cause of the death. In consequence, the dog was diagnosed as necrotizing meningoencephalitis (NME) based on histopathological examination. We would be concerned that tramadol may be related to seizure activity in the NME patient. From this case, it is known that although tramadol has been proven to be a safe and effective agent for the control of pain in veterinary medicine, it would be carefully used to patient with history of neurological diseases including meningoencephalitis, hydrocephalus, and encephalopathy.

• Journal of Veterinary Clinics
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• v.26 no.3
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• pp.226-230
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• 2009

We evaluated the efficacy of $\beta$-hemolytic Streptococcus(S.) iniae vaccine on cultured olive flounder. Three hundred flounders(weight $50{\pm}5$ g) were obtained from two farm at Wando and Taean in the southern and western coast of Korea at May and June 2007, respectively. Twenty of flounders moved in 0.5 tons aquaria in land-marine tank system of National Veterinary Research and Quarantine Service. Seawater was transported from the sea of Inchon in western Korea, and water temperature maintained to $22^{\circ}C$ and $25^{\circ}C$ during the vaccination and challenge test, respectively. We used the formalin-inactivated $\beta$-hemolytic S. iniae vaccine produced by domestic manufacturers. The vaccine was intraperitoneally administered to fish. The vaccinated and control group were challenged with intraperitoneal injection by virulent S. iniae SI-36 isolates with $5.0{\times}10^8$ CFU/fish at 3 weeks after vaccination. We evaluated the vaccine efficacy by calculating numbers of dead fish, and observing of clinical signs, exterior and gross lesions, and examining bacteria isolation and identification. Thirty-four(25.2%) of 135 control and vaccinated group fish were dead with serious anemia, abdominal extension, and hernia of intestine during 3 weeks post vaccination. We isolated Neoheterobothrium hirame from the buccal cavity and Edwardsiella tarda from kidney of dead and diseased fish. When infected fish with these agents were challenged with S. iniae SI-36 isolates, the cumulative mortality of control and vaccinated group were 86.7, and 46.7%, respectively. However, significant differences(p<0.05) were observed on cumulative mortality between control(20.0%) and vaccinated group(95.0%) at second trials with 40 healthy, and relative percent survival(RPS) was 78.0%. We confirmed that the efficacy of $\beta$-hemolytic S. iniae vaccine on olive flounder were impacted by health condition such as bacterial and parasitic diseases.

• Journal of Korean Society on Water Environment
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• v.28 no.5
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• pp.704-716
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• 2012

This paper provides a detailed account of pilot testing conducted at South Lake Tahoe (California), the Ddukdo (Seoul) water treatment plant (WTP) and the Bokjung (Seongnam) WTP between February, 2010, and February, 2012. The objectives were first, to characterize the reactions of ozone with hydrogen peroxide (Peroxone) for Han River water following sand filtration, second to determine empirical ozone and hydrogen peroxide doses to remove a taste-and-odor surrogate 2-methylisoborneol (MIB) using an advanced oxidation process (AOP) configuration and third, to determine the optimum dosing configuration to reduce residual ozone to a safe level at the exit of the process. The testing was performed in a real-time plant environment at both low- and high seasonal water temperatures. Experimental results including ozone decomposition rates were dependent on temperature and pH, consistent with data reported by other researchers. MIB in post-sand-filtration water was spiked to 40-50 ng/L, and in all cases, it was reduced to below the specified target level (7 ng/liter) and typically non-detect (ND). It was demonstrated that Peroxone could achieve both MIB removal and low effluent ozone residual at ozone+hydrogen peroxide doses less than those for ozone alone. An empirical predictive model, suitable for use by design engineers and operating personnel and for incorporation in plant control systems was developed. Due to a significant reduction in the ozone reaction/decomposition at low winter temperatures, results demonstrate the hydrogen peroxide can be "pre-conditioned" in order to increase initial reaction rates and achieve lower ozone residuals. Results also indicate the method, location and composition of hydrogen peroxide injection is critical to successful implementation of Peroxone without using excessive chemicals or degrading performance.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1278-1282
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• 2001

Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on the catabolic response overcome of mice was examined. Female ICR mice (6~7 weeks of age) were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 5 groups (6 mice/cage/treatment). Mice were intubated p.o., AEY (5, 10 and 15 mg), control egg yolk (CEY, 10 mg), or fish oil (5 mg) dissolved in 0.2 mL phosphate buffered saline (PBS) every two days for 14 days. At day 15, the 0.1 mL of lipopolysaccharide solution (LPS, 30$\mu\textrm{g}$/0.1 mL 10 mM HEPES) was injected through tail vein, and then, the body weight of mouse and the amount of feed intake were measured over a period of 72 hours. Control group mice were received only PBS and LPS. AEY treatment suppressed the loss of mice body weight in a dose-response manner. Twenty four hours post LPS injection, the reduced body weight per mouse of AEY 5, AEY 10, and AEY 15 mg treatment groups was 3.70, 3.54, and 3.25 g, respectively. Body weight suppression effect of AEY treatment was greater than that of CEY, but less than fish oil. AEY treatment did not alter thymus weight, but increased the weight of spleen or liver. These results indicate that AEY suppressed the loss of body weight by LPS via any function of the spleen and/or liver.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1283-1286
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• 2001

Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on mouse humoral immunity was investigated using male ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity- controlled house for one week and randomly divided into 5 treatment groups (9 mice/cage/treatment). Mice were intubated p.o., AEY (100, 250 and 500$\mu\textrm{g}$) or control egg yolks (CEY, 250$\mu\textrm{g}$), dissolved in 0.1 mL DMSO, for consecutive 4 days. At day 5, carbon suspension (pilot drawing ink 3 mL+3% gelatine 3 mL) was injected 3 $\mu$L Per 1 g body weight through tail vein. Carbon clearance time was measured at 5 and 35 minutes Post the injection of carbon suspension. Another two experiments were conducted to determine the hemagglultinin-titer (HGT) and hemolysin-titer (HLT) with male ICR mouse (8 mice/cage/treatment). Mice treated with AEY were induced immune activity with SRBC. HGT and HLT were measured from the blood at day 1 and 3 after treatment of SRBC. AEY treatment reduced the carbon clearance time. Especially the carbon clearance time by 500 $\mu\textrm{g}$ AEY treatment was 5.00 minutes, which was very short time compared with 9.42 minutes by control and 9.01 minutes by CEY. AEY group showed slights higher values of HGT and HLT than CEY group and control. At day 1, HGT in control, 250$\mu\textrm{g}$ CEY and 250$\mu\textrm{g}$ AEY groups was 5.50, 5.63, and 6.00, respectively. Similiarly, HLT in control, 250$\mu\textrm{g}$ CEY and 250$\mu\textrm{g}$ AEY groups was 4.75, 5.38, and 5.50, respectively, at day 1. These results suggest that AEY exhibited immunity-enhancing effect.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.3
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• pp.294-301
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• 2008

This study was carried out to investigate the protective effects and detoxification of oriental herb extracts mixture (Saururus chinensis, Taraxacum platycarpum, Ulmus macrocarpa, Glycyrrhiza glabra, Rhynchosia nulubilis) on TCDD-induced oxidative stress. Thirty five male rats were divided into 5 groups: normal control group received saline; vehicle control group received DMSO and acetone; only TCDD-treated group received multiple intraperitoneal injection of TCDD ($100{\mu}g/kg$) and saline; post-treated group of OHEM (400 mg/kg/day) received oral administration for 5 weeks after TCDD treatment; and pre-treated group of OHEM (400 mg/kg/day) received oral administration for 6 weeks from 1 week before TCDD treatment. The elevated serum activities of alanine transaminase (AST), aspartate transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and atherorganic index due to TCDD were significantly decreased by the treatment of OHEM (p<0.05); the pre-treatment of OHEM was especially effective. Hydropic lesions and cytoplasmic vacuolizations in the liver of TCDD-treated rats were inhibited by the treatment of OHEM. Also, OHEM treatment reduced edemas in small intestine villus of TCDD-treated rats. These results suggest that OHEM from various oriental herb extracts might be a useful protective and detoxification agent against TCDD.

• Clinical and Experimental Reproductive Medicine
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• v.38 no.1
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• pp.31-36
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• 2011

Objective: To determine whether the serum ${\beta}$-human chorionic gonadotropin (hCG) profile following preimplantation genetic diagnosis (PGD) is lower than that of intracytoplasmic sperm injection (ICSI) cycles. Methods: A total of 129 PGD cycles and 1,161 age-matched ICSI cycles, which resulted in pregnancy (serum ${\beta}-hCG{\geq}5$ mIU/mL) on post-ovulation day (POD) 12 were included. We compared the mean serum ${\beta}$-hCG levels on POD 12, 14, 21, and 28, doubling time of serum hCG, and created a cut-off value for predicting a singleton pregnancy in each group. Results: The mean serum ${\beta}$-hCG concentration of the PGD group was significantly lower than that of the control group on POD 12, 14, and 21. The doubling time of serum ${\beta}$-hCG at each time interval showed no significant difference. The cut-off-value of serum ${\beta}$-hCG for predicting a single viable pregnancy was 32.5 mIU/mL on POD 12 and 113.5 mIU/mL on POD 14 for the PGD group, which was lower than that for the control group. Conclusion: Blastomere biopsy may decrease the ${\beta}$-hCG-producing activity of the trophoblasts, especially in early pregnancy. Setting a lower cut-off value of serum ${\beta}$-hCG for predicting pregnancy outcomes in PGD may be needed.

• Journal of Embryo Transfer
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• v.12 no.1
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• pp.11-20
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• 1997

The influence of cryopreservation of donor embryos on the in vitro developmental potential in the nuclear transplant rabbit embryos was evaluated. The embryos of 16-cell stage were collected and cryopreserved with EFS solution by vitrification method. The frozen embryos were thawed and synchronized to S and G$_1$ phase of 32-cell stage. The recipient/ cytoplasms were obtained by removing the first polar body and chromosome mass from the oocytes collected by non-disruptive microsurgery procedure. The separated S and G$_1$ phase blastomeres of 32-cell stage were injected into enucleated recipient cytoplasms by micromanipulation. After culture until 20 hrs post-hCG injection, the nuclear transplant oocytes were electrofused and activated by electrical stimulation. The fused nuclear transplant embryos were co-cultured with rabbit oviduct epithelial cells. After in vitro culture for 120 hrs, the nuclear transplant embryos developed to blastocyst stage were stained with Hoechst 33342 dye and their blastomeres were counted. The electrofusion rate was significantly (P<0.05) reduced in the frozen nuclear donor,compared with fresh donor nuclei as 80.0 vs 62.8% in S phase and 81.7 vs 64.8% in G$_1$phase, respectivley. The in vitro developmental rate to blastocyst stage with the S and G$_1$phase of fresh embryos(26.3 and 61.1%, respectively) was found significantly (P<0.05) higher, compared to the S and G]phase of frozen embryos(11.9 and 34.6%, respectively). When frozen as well as fresh donor embryos were synchronized to G$_1$ phase, the in vitro developmental rate to blastocyst stage was significantly (P<0.05) higher, compared with S phase donor nuclei. The cell counts of nuclear transplant embryos developed to blastosyst stage were significantly (P<0.05) more in G$_1$ phase of fresh or frozen embryos (180.1 and 125.7 cells, respectively), compared with S phase nuclear donor (145.1 and 103.7 cells, respectively). From the above results it was concluded that the rabbit embryos cryo- preserved by vitrification might be available as nuclear donor, though the developmentalpotential and cell counts of nuclear transplant rabbit embryos were decreased significantly.