• KSBB Journal
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• v.20 no.3
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• pp.220-227
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• 2005

Doxorubicin is an anthracycline-family polyketide compound with a very potent anti-cancer activity, typically produced by Streptomyces peucetius. To understand the potential target biosynthetic genes critical for the doxorubicin everproduction, a doxorubicin-specific DNA microarray chip was fabricated and applied to reveal the growth-phase-dependent expression profiles of biosynthetic genes from two doxorubicin-overproducing strains along with the wild-type strain. Two doxorubicin-overproducing 5. peucetius strains were generated via over-expression of a dnrl (a doxorubicin-specific positive regulatory gene) and a doxA (a gene involved in the conversion from daunorubicin to doxorubicin) using a streptomycetes high expression vector containing a strong ermE promoter. Each doxorubicin-overproducing strain was quantitatively compared with the wild-type doxorubicin producer based on the growth-phase-dependent doxorubicin productivity as well as doxorubicin biosynthetic gene expression profiles. The doxorubicin-specific DNA microarray chip data revealed the early-and-steady expressions of the doxorubicin-specific regulatory gene (dnrl), the doxorubicin resistance genes (drrA, drrB, drrC), and the doxorubicin deoxysugar biosynthetic gene (dnmL) are critical for the doxorubicin overproduction in S. peucetius. These results provide that the relationship between the growth-phase-dependent doxorubicin productivity and the doxorubicin biosynthetic gene expression profiles should lead us a rational design of molecular genetic strain improvement strategy.

• Korean Journal of Social Welfare
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• v.62 no.1
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• pp.291-316
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• 2010

This study aimed to look for the possibility of dissolving social exclusion about the social vulnerable classes through the culture welfare programs. For this purpose, we analyzed interview records focused on the culture welfare practitioners applying the Modified Grounded Theory Approach worked out by Kinosita. The results showed that the culture welfare programs functionated of dissolving social exclusion about the social weaks by enhancing latent faculties and the sense of self-respect of them through providing various opportunities of culture fruition. It was appeared that the culture welfare programs promoted creative competence and the sense of self-respect, and strengthened the sense of solidarity of the participants by using the approaching strategies of offering various opportunities of creational experience, atypical operating programs centered on the process, establishing of the participants' subjecthood, and communal activities. That is, it was proved that actually the social weaks experienced the change of life with feeling emotional satisfaction, promoting family and human relationship, establishing positive identity, empowerment, participating communal activities, and so on, through the culture welfare programs. From these results we can know that if we provide the programs mixing the culture welfare programs with social welfare services which traditionally reinforced social exclusion about the social vulnerable classes by stigma, the social exclusion about them can be dissolved.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.31 no.3
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• pp.219-227
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• 2005

Purpose: Abnormalities in the p53 gene are regarded as the most consistent genetic abnormalities detected in head and neck squamous cell carcinogenesis. Two new members of the p53 gene family, p73 and p63 have recently been identified. They share considerable sequence homology with p53 in the transactivation, DNA binding, and oligomerization domains, indicating possible involvement in carcinogenesis. Disruption of the homeostatic balance between proliferation and apoptosis is widely believed to contribute to human oral carcinogenesis. The aim of this study was to analyze expression of p63 in squamous cell carcinogenesis and to compare with immunochemical markers representing cell proliferation and apoptosis. Materials and Methods: Using the Syrian hamster oral cancer model, the fraction of apoptotic (apoptotic index-AI), proliferating (mitotic index-MI) and p63 expressing keratinocytes were examined at normal, dysplastic and malignant oral epithelium using the TUNEL assay, PCNA and p63 immunostaining. Results: p63 significantly increased between normal and dysplastic epithelium and between dysplastic and malignant epithelium. PCNA significantly increased between normal and dysplastic epithelium and between normal and malignant epithelium. However, increase between dysplastic and malignant epithelium, though still increasing, was not statistically significant. The percentage of TUNEL positive cells increased from normal to dysplastic epithelium and returned to normal keratinocyte level in the malignant epithelium. However, differences between tissue types were not significant. The ratio of MI:AI increased significantly only in the dysplastic-malignant epithelial transition. The increase of p63 expression closely reflected the change in the MI:AI ratio during oral carcinogenesis. Conclusion: The p63 may be associated with the regulation of epithelial proliferation and apoptosis in DMBA-induced hamster buccal pouch squamous cell carcinogenesis. Further study is required to investigate which p63 isoforms are involved in hamster buccal pouch carcinogenesis.

• Korean Journal of Social Welfare
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• v.50
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• pp.3-32
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• 2002

The purpose of this study was to introduce Self-Empowerment Program focusing on empowering social workers in individual level and to evaluate effectiveness of the program through practicing it. For the purpose, this study reviewed the concept of self-empowerment, theoretical background and content of the program. It examined effects of the program on mental health social workers' self concepts and clients' evaluation of the social workers, also. Through applying the program and analysing it, some significant differences were found in total positive self, self identity, self satisfaction, self behavior, moral ethical self, personal self, and family self score between intervention group and control group after the program. In evaluation of the social workers by the clients, the score of social workers in intervention group was increased after the program than control group's. These findings suggested that this program be helpful for social workers' self-empowerment and relationship with clients. It is meaningful to prove utility of the program in our social work situation with limited training or growing opportunities for social workers.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.10
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• pp.6036-6045
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• 2014

This survey research was performed to determine the social capital that public officials generate within the relationship of the community at the individual level by performing their public office work as an administrative duty. To this end, a survey was conducted on 500 civil servants in Jeju province. The survey period was May 19-23, 2014. 476 subjects were analyzed using a T-test, One way ANOVA, and multiple regression analysis to on SPSS 19.0Win software. As a result of the analysis, 1. The norms of the community cultural, male staff participation and cooperation, and social capital level of civil servants, showed a significantly higher association than female civil servants. There was a difference between the populations of registration to an association to the work location type. In addition, the social capital increases generally as the level of the officers increases. 2. The sub-category in the social capital of a personal level on civil servants family & associates trust, participation & cooperation and neighbor trust had a positive impact on the efficiency of public services. On the other hand, community cultural associations (network) did not have an influence on efficiency.

• Journal of Life Science
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• v.23 no.2
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• pp.182-189
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• 2013

Halophytes are unique land plants that are capable of thriving in a high-salt environment. They are attracting public attention due to their ability to synthesize bioactive substances such as UV protectants or antioxidizing agents. To achieve unaffected growth under high salinity, halophytes may take advantage of the activities of cell growth factors such as expansins. Expansins are well-known cell wall proteins that are responsible for cell enlargement. They loosen cell walls, thereby contributing to actual plant growth. This study aimed to identify positive roles of expansins in the growth of halophytes. Three expansin cDNA clones were isolated from seedlings of Suaeda japonica. Comparing the deduced amino acid sequences of the expansin genes of S. japonica with those of other plant species suggested that the cDNA clones isolated from S. japonica belong to the EXPA (${\alpha}$-expansin) gene family. A phylogenetic tree based on the deduced amino acid sequences revealed that the expansins of S. japonica share a close evolutionary relationship with those of strawberry (Fragaria ananassa) and jujube (Ziziphus jujuba), both of which are woody dicots. SjEXPAs did not show any remarkable change in the gene expression level in different NaCl concentrations, providing a clue to the unaffected seedling growth of S. japonica in a high-salt environment. In conclusion, the present study presents the first report of expansin genes from halophytes and suggests a putative role for these genes in plant growth under high salinity.

• 한국체육학회지인문사회과학편
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• v.56 no.1
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• pp.53-65
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• 2017

This study was conducted to examine how marine sport event visitors' satisfactions and city image effect on revisit and recommendation. To do this, we analyzed the socio-statistical characteristics of spectators, and examined the influence relationship among city image, satisfaction, revisit and recommendation intention. The results were as follows: First, 'family and relatives' as the companions of spectators of yacht competition were the most, and the route of acquiring the competition information was 'the other', in other words, that most of the respondents learned directly in the field, and 'car' as transportation means was the most. In addition, "local tourism" as watching purposes was the most and 'the day' as the period of stay was the most. Second, the image and satisfaction of spectators of yacht competition showed significant influence on revisit and recommendation intention. Specifically, program satisfaction and program agent satisfaction, which are a sub-factors of satisfaction, have a significant positive effect on revisit and recommendation intention. Through these research results, we were able to confirm inadequacy(lack of attractiveness, connections and public relations) of this event, and see what parts should be improved in order to be born again a sustainable event.

• 한국노년학
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• v.32 no.3
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• pp.729-745
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• 2012

The purpose of this study is to review person-centered care models and the scientific evidence for the impact of person-centered care in U.S. nursing homes, and to analyze the applicability of person-centered care for Korean long-term care system. The most commonly recognized person-centered care models were Eden Alternative, Green House, and Wellspring model. The core components of the three models were autonomy, close relationships, homelike environment, and staff empowerment. Thirty eight empirical studies including 10 model testing studies and 28 studies to examine the impact of person-centered care interventions indicated generally positive outcomes in terms of residents, family, and staff/organization level. The authors analyzed the current situation in the Korean long-term care system with respect to the specific core constructs of person-centered care, and proposed applicable models and practical suggestions in consideration of Korean context. Lastly, future considerations regarding policy and research were discussed.

• Korean Journal of Legislative Studies
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• v.24 no.1
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• pp.5-29
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• 2018

This paper aims at making an assessment about the first year of the Moon Jae-in government. President Moon was elected amid political insecurity over the impeachment of then-president Park Geun-hye. However, the Moon administration settled in fairly smoothly despite no transition period of presidential power. Political and economic stability was soon restored, and the tensions between the US and North Korea over the North's nuclear weapons program were, to some extent, managed along with the agreement of the North-South summit and the US-North Korea summit. Pyeongchang hosted the 2018 winter olympics and paralympics successfully. Moon continued very positive approval ratings of higher than 70%. However, he has something to be desired. His leadership seems to rely heavily on a limited number of close staff in the Blue House, alienating the governing the Minjoo Party of Korea. He should build better relationship with opposition parties particularly given a divided government. Rectification of the lingering negative practices should also produce institutional solutions. Above all, Moon should carefully watch out to prevent scandals over corruption and power abuse around him and his family, which would lead to a precipitous decline of the approval ratings and the abrupt weakening of the leadership.

• Korean Journal of Culture and Social Issue
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• v.26 no.3
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• pp.303-327
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• 2020

Previous studies have examined the relationship between domain satisfaction and life satisfaction. However, a comprehensive investigation of satisfaction with multiple domains and their relative contributions to life satisfaction and hedonic balance are missing in the literature. And most studies were conducted in English speaking countries and only a few cross-cultural studies have been conducted. In the current research, we compared Korean and European Canadian university students to examine how domain satisfactions (satisfaction with healthy lifestyles, family relationships, appearance, financial situation, academic performance) are associated with life satisfaction and hedonic balance. We then examined the relative contributions of people's satisfaction ratings on the life domains to their life satisfaction and hedonic balance. Positive correlations were observed between satisfaction with each of the five life domains, and life satisfaction and hedonic balance across the two cultural groups. Interestingly, satisfactions with healthy lifestyles was the dominant predictor of Koreans' life satisfaction and hedonic balance. Satisfaction with appearance was the dominant predictor of European Canadians' life satisfaction and hedonic balance followed by satisfaction with healthy lifestyles. Overall, these results suggest that there are common life domains that contribute to subjective well-being and that there are specific life domains that may contribute more to subjective well-being depending on the culture.