• Mycobiology
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• 제42권4호
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• pp.339-345
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• 2014

During 2011~2013, a total of 729 samples for 19 types of medicinal plant were collected from Seoulyekryungsi in Seoul, Korea, and investigated for the presence of aflatoxins. The samples were analyzed using immunoaffinity column cleanup and high-performance liquid chromatography coupled to a fluorescence detector after post-column derivatization. Aflatoxins were found in 124 out of the 729 analyzed samples: 65 containing aflatoxin B1 (AFB1), 24 with aflatoxin B2 (AFB2), 15 with aflatoxin G1 (AFG1), and 20 samples with aflatoxin G2 (AFG2). The ranges for positive samples were $0.1{\sim}404.7{\mu}g/kg$ for AFB1, $0.1{\sim}10.0{\mu}g/kg$ for AFB2, $0.1{\sim}635.3{\mu}g/kg$ for AFG1, $0.1{\sim}182.5{\mu}g/kg$ for AFG2, and $0.1{\sim}1,043.9{\mu}g/kg$ for total aflatoxins. Most of the medicinal plant samples (721, 98.9%) were below legal limits, but 8 samples exceeded the legal limits of 10 and $15{\mu}g/kg$ established by the Korean standard for AFB1 and total aflatoxins (the sum of AFB1, AFB2, AFG1 and AFG2), respectively.

• 한국실내디자인학회논문집
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• 제16권3호
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• pp.119-129
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• 2007

This study illustrates a prototype proposal and its evaluation for the development of floor plans in the condominium housing remodeling. For this I evaluated the survey of the residents of the 'K' town in Joonge-dong, Nowon-gu, Seoul, against the proposal which was developed based on the resident's pre-survey. The result includes that the residents' preference for community actions such as the removal of fences which are reviewed as negative, whereas the introduction of a fitness center and community facilities in the library are reviewed as positive. The residents preferred the open space by the piloti structure, although which is the wall column structure, where they anticipated a rest area, sports facilities, and bicycle drop-off spots. The sidewall proposal in the main building and the full use of the roof floor were underestimated in terms of design concept. The research also illustrated the residents' desired space planning options, those tending to use of space 'B type', personalization 'C type' and spatial size 'A type'. Residents responded positively to the introduction of the 'Alpha' space in which two households share one space that could be used for multiple purposes such as a rest area, an environment-friendly garden, and hobby room, etc, and also there is another possibility it plays an important role to the sociality in community.

• Journal of Microbiology
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• 제45권3호
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• pp.262-267
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• 2007

During a screening program, an actinomycete strain isolated from the Egyptian soil was investigated for its potential to show antimicrobial activity. The identification of this isolate was performed according to spore morphology and cell wall chemo-type, which suggested that this strain is a streptomycete. Further cultural, physiological characteristics and the analysis of the nucleotide sequence of the 16S rRNA gene (1480 bp) of this isolate indicated that this strain is identical to Streptomyces violaceusniger (accession number EF063682) and then designated S. violaceusniger strain HAL64. In its culture supernatant, this organism could produce one major compound strongly inhibits the growth of Gram-positive but the inhibition of Gram-negative indicator bacteria was lower. The antibiotic was separated by silica gel column chromatography and then purified on a sephadex LH-20 column and finally the purity was checked by HPLC. The chemical structure of the purified compound was determined using spectroscopic analyses (molecular formula of $C_{33}H_{32}N_{2}O_{10}$ and molecular weight of 617.21) and found to be identical to the kosinostatin, a quinocycline antibiotic which is known to be produced by Micromonspora sp. TP-A0468 (Igarashi et al., 2002) and to quinocycline B isolated from Streptomyces aureofaciens (Celmer et al., 1958). Although the antibiotic is known, the newly isolated strain was able to produce the antibiotic as a major product providing an important biotechnological downstream advantage.

• Journal of Microbiology and Biotechnology
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• 제7권6호
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• pp.402-408
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• 1997

The antibiotic-producing strain HW-003 was screened from soil and found to be effective against the multidrug-resistant Staphylococcus aureus. The spore chain of HW-003 was retinaculiaperti, and the spore surface was spiny. Strain HW-003 has a LL-diaminopimelic acid isoform in the cell wall. The aerial mass color of the strain was gray, and the reverse side was yellow-brown. The strain produced melanin, but did not produce soluble pigments. According to the Taxon program, HW-003 showed best match with Streptomyces cyaneus. Antibiotic production reached a maximum after 72-h cultivation. The antibiotic was purified with silica gel column chromatography, octadecylsilyl column chromatography, and HPLC. The purified antibiotic, AMRSA1, showed strong inhibitory activity against multidrug-resistant Staphylococcus aureus and gram-positive bacteria. The molecular weight of AMRSA1 was about 1, 100. AMRSA1 was a peptide antibiotic containing alanine and serine.

• 한국환경농학회지
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• 제30권4호
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• pp.432-439
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• 2011

BACKGROUND: An official analytical method was developed to determine etofenprox residues in agricultural commodities using high-performance liquid chromatography (HPLC). METHODS AND RESULTS: The etofenprox residue was extracted with acetone from representative samples of five raw products which comprised rice grain, apple, mandarin, cabbage, and soybean. The extract was then serially purified by liquid-liquid partition and Florisil column chromatography. For rice and soybean samples, acetonitrile/n-hexane partition was additionally coupled to remove nonpolar lipids. Reversed phase HPLC using an octadecylsilyl column was successfully applied to separate etofenprox from co-extractives. Intact etofenprox was sensitively detected by ultraviolet absorption at 225 nm. Recovery experiment at the quantitation limit validated that the proposed method could apparently determine the etofenprox residue at 0.02 mg/kg. Mean recoveries from five crop samples fortified at three levels in triplicate were in the range of 93.6~106.4%. Relative standard deviations of the analytical method were all less than 10%, irrespective of crop types. A selected-ion monitoring LC/mass spectrometry with positive atmospheric-pressure chemical ionization was also provided to confirm the suspected residue. CONCLUSION(s): The proposed method is simple, rapid and sensitive enough to be employed in routine inspection or monitoring of agricultural products for the etofenprox residue.

• Coupled systems mechanics
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• 제3권4호
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• pp.367-384
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• 2014

The study deals with the physical modeling of a typical single storeyed building frame resting on pile foundation and embedded in cohesive soil mass using the finite element based software SAP-IV. Two groups of piles comprising two and three piles, with series and parallel arrangement thereof, are considered. The slab provided at top and bottom of the frame along with the pile cap is idealized as four noded and two dimensional thin shell elements. The beams and columns of the frame, and piles are modeled using two noded one dimensional beam-column element. The soil is modeled using closely spaced discrete linear springs. A parametric study is carried out to investigate the effect of various parameters of the pile foundation, such as spacing in a group and number of piles in a group, on the response of superstructure. The response considered includes the displacement at the top of the frame and bending moment in columns. The soil-structure interaction effect is found to increase the displacement in the range of 38 -133% and to increase the absolute maximum positive and negative moments in the column in the range of 2-12% and 2-11%. The effect of the soil- structure interaction is observed to be significant for the type of foundation and soil considered in this study. The results obtained are compared further with those of Chore et al. (2010), wherein different idealizations were used for modeling the superstructure frame and sub-structure elements (foundation). While fair agreement is observed in the results in either study, the trend of the results obtained in both studies is also same.

• 콘크리트학회논문집
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• 제15권1호
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• pp.11-16
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• 2003

현재 국내에서 설계 및 시공되고 있는 비내진상세를 가진 RC구조물의 이력거동특성과 내진성능이 밝혀져 있지 않다. 따라서, 본 연구에서는 일반적인 10층 철근콘크리트 구조물의 외부 보-기둥접합부를 선정하여 슬래브의 유무, 보하부 주근의 접합부내로의 정착방항, 접합부내 보강근의 유무에 따라 6개의 1/3축소 실험체를 만들어 반복횡하중 실험을 수행하였다. 실험결과 슬래브가 있는 실험체가 없는 실험체에 비해 정모멘트일 때 25%, 부모멘트일 때62%의 큰 강도를 나타냈고, 보하부 주근이 상부로 정착된 것이 하부로 정착된 경우보다 정모멘트일 때 8 %, 부모멘트일 때 11% 강도 증가효과가 있었다. 그리고 접합부내에 전단보강근이 보 주근의 뽑힘을 억제하는 것으로 나타났다.

• 한국콘텐츠학회논문지
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• 제14권3호
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• pp.346-351
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• 2014

안전한 수혈을 위한 정확한 혈액형 검사는 필수적이다. 최근에는 혈액은행 검사 분야에 검사의 오류를 줄이고 검사의 효율을 높이기 위해 자동화 장비가 도입되어 사용되고 있으나 중소병원에서 이용하기에는 매우 고가로 도입하기가 어렵다. 이에 본 연구에서는 혈액형 검사결과를 저장 및 재확인 할 수 있고, 이미지 프로세싱에 의해 결과가 판독되는 원주응집법을 이용한 혈액형 검사 판독기를 개발하였다. ABO 및 RhD 혈액형 검사가 의뢰된 148개의 검체와 비예기항체 검사가 의뢰된 154개의 검체를 대상으로 판독 결과를 비교한 결과, 양성과 음성의 판독 및 반응강도의 판독이 100%일치하였다. 본 연구에서 개발된 판독기는 추가적인 검증을 거친다면 중소형 병원에서도 쉽게 도입하여 효율적이며, 경제적으로 이용할 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1191-1196
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• 2009

The production of Streptomyces griseus trypsin (SGT) by S. griseus IFO13350 transformed with the expression vector pWHM3-TR1R2, containing sprT encoding SGT and the two positive regulatory genes sgtR1 and sgtR2, was investigated in various media. Cultivation in Ferm-0 gave 1.4 times more trypsin activity than in C5/L medium. In addition, replacement of 2% glucose and 1% skim milk in Ferm-0 with 2% dextrin and 1% tryptone (designated Ferm-II) enhanced trypsin activity 4.1-fold. To simplify the purification process, the supernatant from the S. griseus transformant cultured in Ferm-II medium was fractionated with ammonium sulfate (25-55%), then subjected to Hitrap Benzamidine FF affinity column chromatography. The specific activity of SGT purified by one-step chromatography was 69,550 unit/mg protein and the overall purification yield was above 8%, indicating that this method is more effective than those previously reported. Purified SGT was most active at pH 8.0 and $50^{\circ}C$, and it maintained activity between pH 7.0 and 9.0 and at temperatures up to $70^{\circ}C$. These enzymatic properties are very similar to those of authentic eukaryotic trypsin purified from bovine pancreas.

• 한국융합학회논문지
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• 제11권7호
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• pp.361-368
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• 2020

본 연구는 근육통 환자들의 척주온열마사지 기기 사용이 근육통 개선에 효과가 있는지를 살펴보고, 정서적 부분인 우울감, 스트레스 개선에도 효과가 있는지 검증하는데 목적이 있다. 이를 위해 연구참여자 16명(남자, 31.25%)은 온열 마사지 기기에 대한 처치로 4주간 주 5회, 1회당 40분을 실시하였다. 연구결과, 주관적 통증정도는 VAS -46.32%, PDI -44.86%의 변화율을 보였으며, VAS, PDI 모두 유의한 감소를 보였다. 기타 우울감 및 스트레스 정도에서는 BDI -21.84%, SRI -11.48%의 변화율을 보였으며, BDI, SRI 모두 유의한 감소를 나타내었다. 따라서 근육통 환자 치료 시 온열마사지 기기를 활용한다면 주관적 근육통, 우울감 및 스트레스 개선에서 긍정적인 효과가 있을 것으로 기대된다.