• Title/Summary/Keyword: Position Identification

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Polymorphism Analysis and Expression of the CCAAT/Enhancer Binding Protein β(C/EBPβ) in the Korean Native Cattle and Black Cattle Storage (한우와 흑한우 CCAAT/Enhancer Binding Protein β(C/EBPβ) 유전자의 발현과 다형분석)

  • Kim, Hey-Min;Lee, Sang-Mi;Pack, Hyo-Young;Yoon, Seul-Ki;Yoon, Du-Hak;Lee, Seung-Soo;Ko, Moon-Suck;Moon, Seung-Ju;Kang, Man-Jong
    • Journal of Animal Science and Technology
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    • v.50 no.2
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    • pp.265-272
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    • 2008
  • The CCAAT/enhancer binding protein β(C/EBPβ), a member of the leucine zipper DNA-binding protein of transcription factor, plays a crucial role in the control of early phases of adipocyte differentiation. In this studies, we report the identification, characterization, and expression of the Korean native cattle C/EBPβ gene. The Korean native cattle and black cattle C/EBPβ cDNA includes a 1047bp open reading frame encoding a protein of 348 amino acids. The C/EBPβ cDNA sequence of the Korean native cattle and black cattle shows high conservation with the corresponding amino acid sequences reported in other species. The distribution of C/EBPβ mRNA in various tissues of Korean native cattle aged 26 months was investigated using Northern Blot analysis. The C/EBPβ expression was detected in adipose tissue, lung, sirloin while expression was not detected in heart, kidney, small intestine, colon, and liver. However, we are analyzed polymorphism of bZIP domain in the C/EBPβ gene. A polymorphism was not identified at this position.

Microstructural Characterization of $Al_3$(${Nb_{1-x}}{Zn_x}$) Alloy Prepared by Elemental Powder and Intermetallic Powder (원료분말과 금속간화합물 분말로 기계적 합금화한 $Al_3$(${Nb_{1-x}}{Zn_x}$) 합금의 미세구조특성)

  • Lee, Gwang-Min;Lee, Ji-Seong;An, In-Seop
    • Korean Journal of Materials Research
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    • v.11 no.5
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    • pp.345-353
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    • 2001
  • The present study was carried out to investigate the effect of zirconium addition to $Al_3$Nb intermetallic on the crystal structural modification and microstructural characterization of $Al_3$Nb intermetallic. Elemental Al, Nb, Zr powders and arc melted $Al_3$Nb and $Al_3$Zr intermetallic mixed powders were used as starting materials. MA was carried out in an attritor rotated with 300 rpm for 20 hours. The behavior of MA between two starting materials was some-what different in which the value of internal strain of the elemental powders was higher than that of the intermetallic powder. The intermetallic powder was much more disintegrated during the MA processing. In the case of the elemental powders, AlNb$_2$ phase were transformed to Al(Nb.Zr)$_2$ as a result of ternary addition of Zr element. With the successive heat treatment at 873K for 2 hours, the Al(Nb.Zr)$_2$ phase was transformed to more stable $Al_3$(Nb.Zr) phase. This transformation was clearly confirmed by the identification of X-ray peak position shift. On the other hand, in the carte of the intermetallic powder, there was no evidence of phase transformation to other ternary intermetallic compounds or amorphous phases, even in the case of additional heat treatment. However, nano-sized intermetallic with $Al_3$Nb and $Al_3$Zr were just well distributed instead of phase transformation.

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Identification of catalytic acidic residues of levan fructotransferase from Microbacterium sp. AL-210 (Microbacterium sp. AL-210이 생산하는 levan fructotransferase의 효소활성에 중요한 아미노산의 동정)

  • Sung, Hee-Kyung;Moon, Keum-Ok;Choi, Ki-Won;Choi, Kyung-Hwa;Hwang, Kyung-Ju;Kim, Myo-Jung;Cha, Jae-Ho
    • Journal of Life Science
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    • v.17 no.1 s.81
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    • pp.6-11
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    • 2007
  • [ $\beta$ ]-Fructofuranosidases, a family 32 of glycoside hydrolases (GH32), share three conserved domains including the W(L/M)(C/N)DP(Q/N), FRDPK, and ECP(D/G) motifs. The functional role of the conserved acidic residues within three domains of levan fructotransferase, one of the $\beta-fructofuranosidases$, from Microbacterium sp. AL-210 was studied by site-directed mutagenesis. Each mutant was overexpressed in E. coli BL21(DE3) and purified by using Hi-Trap chelating affinity chromatography and fast performance liquid chromatography. Substitution of Asp-63 by Ala, Asp-195 by Asn, and Glu-245 by Ala and Asp decreased the enzyme activity by approximately 100-fold compared to the wild-type enzyme. This result indicates that three acidic residues Asp-63, Asp-195, and Glu-245 play a major role in catalysis. Since the three acidic residues are present in a conserved position in inulinase, levanase, levanfructotransferase, and invertase, they are likely to have a common functional role as nucleophile, transition state stabilizer, and general acid in $\beta-fructofuranosidases$.

Parenthood (어버이살이)

  • Cho, Doo-Young
    • Korean Journal of Psychosomatic Medicine
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    • v.5 no.1
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    • pp.3-11
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    • 1997
  • In becoming parents, the marital partners enter into a new developmental phase. The conception of the child is an act of mutual creativity during which the boundaries between the self and another were temporarily obliterated more completely than at any time since infancy. The infant is a physical fusion of the parents, and their personalities unite within the child. for many women, creativity as a mother becomes a central matter that provides meaning and balance to their lives. The husband usually has strong desires for an offspring and can be transformed by it. The child can profoundly affect one or both parents, and the influences are reciprocal-a child's needs or specific difficulties uncover a parent's inadequacy. following the child's development, each transition into a new developmental phase requires an adaptation by the parents, and one or another of these required adaptations may disturb a parent's equilibirium. And the personality changes, emotional difficulties, and regressions of a spouse that occur in response to some phase of parenthood can upset the marriage. Not only do children identify with parents, but parents also identify with their children. The parents take pleasure in child's joy and suffer with the child's pain more than in almost any other relationship. certain respects e parents lives again in the child. Through the process of identification the child can also provide one of the two parents with the opportunity to experience intimately the way in which a person of the opposite gender grows up. Parenthood also provides the opportunity to be loved, admired, and needed simply because one is a parent and, as such, a central and necessary object in the young child's life. The many potentialities for emotional satisfactions from parenthood manage to outweigh the tribulations and sacrifices that are required. The child also exerts an indirect effect through changing the parent's position in the society, for new sets of relationships are established as the parents are drawn to other couples with children of the same age, and for a new impetus toward economic and social mobility often possesses the parents. frequently the couple's relatedness to their own parents improves and grows firmer once again. Parenthood, the satisfactions it provides and the demands it makes, varies as life progresses : and changes with the parent's interests, needs, and age as well as with the children's maturation. There are phases in the child's life that the parents are reluctant to have pass, whereas they tolerate others largely through knowing that they will soon be over. The changing lives of the children provide many satisfactions that offset the tribulations, uncertainties, and regrets. The parents change. The young father, who was just starting on his carrier whom the first child was born, settles into a life pattern. He becomes secure with increasing achievement and interacts differently with the youngest child and provides a different model for him than for the oldest. The mother may have less time for a second or third child than for her first, but she may also be more assured in her handling of them. The birth of a baby when the parents art in their late thirties will find them Less capable of physical exertion with the child and less tolerant of annoyances, but they are less apt to be annoyed. Eventually the children min and leave home, but the couple do not cease to be parents.

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Study on Development Method for Galileo/SAR Ground System in Korea (국내의 갈릴레오 탐색구조 지상시스템 개발 방안에 관한 연구)

  • Ju, In-Won;Lee, Sang-Uk;Kim, Jae-Hun
    • Journal of Satellite, Information and Communications
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    • v.2 no.1
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    • pp.35-40
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    • 2007
  • COSPAS-SARSAT is the search and rescue system for providing a distress alarm and a position identification using an international satellite and ground facilities. Aviators, mariners and land users worldwide are equipped with COSPAS-SARSAT distress beacons, which could help save their in emergency situations anywhere in the world. As the existing COSPAS-SARSAT system is generally operated by LEO(Low-altitude Earth Orbit) Satellite System, the time from the distress beacon to the rescue is more than 1 hour with average and the accuracy of the distress location is about 5 Km. Therefore, in order to overcome this problem, the development for the next generation SAR(search and rescue) system which uses the MEO(middle-altitude Earth Orbit) satellites is going on the Galileo project. EU is developing this project for the full operation capability in 2011, and this project will have SAR payloads and support to the Search and Rescue service-herein called SAR/Galileo. SAR/Galileo will have the performance of a few meter accuracy, within 10 minutes to rescue from reception of distress messages, and Return Link Service(from the SAR operator to the distress emitting beacon), thereby facilitating more efficient rescue operations and helping to reduce the rate of false alerts. As the disaster is larger every year, the ground station, MEOLUT for next generation ASR/Galileo is urgently needed for the lifesaving for the larger disaster, the research for beacon and the ground station such as MEOLUT for introducing the next generation SAR/Galileo in Korea is very timely and is important. This paper presents the procedures and the strategies for the participation, the area to develop reasonably, and the propulsion organization for developing the SAR/Galileo ground system in Korea.

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Detection and Analysis of the Liver Region and Hepatoma in CT Images Using Shape-based Interpolation and Quantization Method (형태기반 보간법과 양자화 기법을 이용한 CT 영상에서의 간 영역과 간암 추출 및 분석)

  • Kim, Kwang-Baek
    • Journal of the Korean Institute of Intelligent Systems
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    • v.17 no.3
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    • pp.380-389
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    • 2007
  • In Korea, undoubtedly, the cancer is one of the most common reasons of death, and hepatoma is the second highest fatal cancer regardless of the gender only next to the stomach cancer In the middle and prime-aged between 40 and 60 years, the incidence of hepatoma is the highest in the world, and the death rate due to hepatoma is the highest among OECD countries. In this paper, we propose a novel method for automatic identification of hepatoma from a contrast enhanced CT images, which is used in an expert system that helps medical specialists. First, consecutive $40{\sim}50$ contrail enhanced CT images are photographed by every 5mm from the upper part of the chest, and using position information on the rib, we classify the internal area including only internal organs and the external one that consists of the rib, subcutaneous fat layers, and the background from the CT images. Then, the region of the liver is extracted from the classified internal area by using information on the intensity, the distribution of brightness, and using the regions extracted from consecutive images, we restore information on the 5 mm space occurred between the consecutive two slides tty applying a shape-based interpolation method. Lastly, using the characteristics such as the brightness and the morphology, we are able to extract the regions of hepatoma. The expert system based on our method is sufficiently competitive when it is compared with the diagnoses by specialists in the diagnostic radiology.

Development of Automatic Sorting System for Black Plastics Using Laser Induced Breakdown Spectroscopy (LIBS) (LIBS를 이용한 흑색 플라스틱의 자동선별 시스템 개발)

  • Park, Eun Kyu;Jung, Bam Bit;Choi, Woo Zin;Oh, Sung Kwun
    • Resources Recycling
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    • v.26 no.6
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    • pp.73-83
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    • 2017
  • Used small household appliances have a wide variety of product types and component materials, and contain high percentage of black plastics. However, they are not being recycled efficiently as conventional sensors such as near-infrared ray (NIR), etc. are not able to detect black plastic by types. In the present study, an automatic sorting system was developed based on laser-induced breakdown spectroscopy (LIBS) to promote the recycling of waste plastics. The system we developed mainly consists of sample feeder, automatic position recognition system, LIBS device, separator and control unit. By applying laser pulse on the target sample, characteristic spectral data can be obtained and analyzed by using CCD detectors. The obtained data was then treated by using a classifier, which was developed based on artificial intelligent algorithm. The separation tests on waste plastics also were carried out by using a lab-scale automatic sorting system and the test results will be discussed. The classification rate of the radial basis neural network (RBFNNs) classifier developed in this study was about > 97%. The recognition rate of the black plastic by types with the automatic sorting system was more than 94.0% and the sorting efficiency was more than 80.0%. Automatic sorting system based on LIBS technology is in its infant stage and it has a high potential for utilization in and outside Korea due to its excellent economic efficiency.

Epidemiological and Lumbar x-ray Studies on the Low Back Pain of the Workers in an Automobile Industry (자동차 제조업체 근로자들의 요통에 대한 역학적 요추 x-선학적 고찰)

  • Kim, Soon-Lae
    • Research in Community and Public Health Nursing
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    • v.6 no.2
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    • pp.319-334
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    • 1995
  • To investigate the risk factors of low back pain, an epidemiological study was carried out among male workers aged 20-55 employed in an automobile industry in Korea during the time period from February 1993 to October 1995. Workers participated to this study were divided into low back pain group(LBP) and control group, according to the self-reports by written questionnaires. General characteristics, medical history, work related factors, fatigue, and MMPI were compared between two groups. To clarify the relationship between job related low back pain and radiologic features of lumbar spine, radiographic study was carried out. The resultant data were processed for $x^2-test$, t-test, and stepwise logistic regression to confirm the adjusted odds ratios. The results were as follows: 1. History of back disease, lifting and carrying work, excessive physical fatigue, and weakend back strength of individual workers were directly associated with low back pain. Odd ratios of these 4 risk factors of low back pain were 5.07, 3.34, 1.49, and 1.22 respectively. 2. The frequency of low back pain history was significantly higher in LBP group. 3. Back muscle strength of lumbar spine of LBP group were significantly lower than control group. 4. The workers in LBP group revealed high fatigue symptoms. 5. In MMPI test LBP group showed higher scales in hypochondriasis, depression, hysteria, psychopathic deviate, paranoia, psychasthenia, schizophrenia, and hypomania. 6. LBP group were more frequently involved in lifting and carrying, working in awkward position, bending, twisting and using lower extremities. 7. LBP group were exposed more to vibration during working. 8. In the Analysis of radiographs of lumbar spine, Jacob's line not crossing fourth lumhar disc space, transitional vertebrae and lumbar displacement more than 4.4mm in standing lateral view were more frequently observed in LBP group than control group. Through these results, it is concluded that identification of previous history of back problem, change of work or working environment for workers with previous back problem and measures to relieve both physical and psychological fatigue of the workers are required for optimal management of work-related back problems among workers. In the present study, several results were different from the previous reports: Jacob's line not corssing fourth lumbar disc space, lumbarization, and vertebral slipping (spondylolisthesis) more than 4.4mm are related to backache. Meticulous studies are required to elucidate the difference.

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An Instructional Model for the Improvement of Decision Making Skills in the 'Technological Innovation and Invention' Unit of Technology·Home Economics Curriculum (고등학교 기술·가정과 '기술 혁신과 발명' 단원에서 의사결정능력 향상을 위한 수업 모형 개발)

  • Seo, Joung-Wan;Lee, Yong-jin
    • 대한공업교육학회지
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    • v.41 no.1
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    • pp.108-127
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    • 2016
  • We confront incessant choices in life. A person or a group stands at a crossroad continuously and make decisions under limited conditions in each time in order to achieve a goal. These series of processions are referred to as decision making and dominates our actions. Based on this view, the decision making is crucial and it secures its own position in school education as well as in real life. The purpose of this study is to develop decision making skills instructional model that can be applied to improve decision making skills in technological subjects. The components of decision making skills in technological education was problem recognition, problem identification, alternative production, alternative assessment, optimum selection, optimum evaluate. An instructional models for improvement of decision making skills in technology education and examination was designed according to the components of decision making skills in technological education. Depending on the components of decision making skills developed a teaching material to experience each step. And in the teaching material was to verify the improved decision-making skill. Accordingly, a decision making instruction model in technology is expected to contribute to enhance students' decision making skills.

Space Design Expression Method According to the Analysis of the Characteristics of Movies - Focused on the Characteristics Expressive of the Illusionist - (영화의 특성 분석에 따른 공간디자인의 표현방법 - 환영성 표현 특성을 중심으로 -)

  • 송춘의
    • Archives of design research
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    • v.17 no.1
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    • pp.231-240
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    • 2004
  • Space design in the 21st century has been developed in connection with media more various than ever. In the age of image represented by popular culture, space design connected with media is based on post-reason thoughts constituting the post-modern society, and reflects polysemously and pluralistically changes in the society through the identification of art with life. The movie, which is an image perceived particularly sensibly and the most impractical simulated image among various illusionary media images composing the contemporary society, is growing rapidly in interrelation with various fields of art based on its peculiar nature, and its influence is getting more extensive. The movie manipulates mass society to create what does not exist and restore lost images into new realities through the reproduction of extremely realistic senses, and uses them in establishing virtual realities and creating illusionary images representing the age of simularc. The illusionary expression of the movie produces new meanings through disintegrating existing meanings and recombining them, and expresses popular culture from a position closest to our everyday life. Such an analytical attitude toward the illusionary movie reflecting our society can be a new approach to space design. The present study attempts to suggest the possibility of applying such an illusionary characteristic of the movie as a methodology of space design by utilizing the characteristic as a conceptual language of space design.

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