• 한국양식학회지
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• 제13권4호
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• pp.353-357
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• 2000

Porphyra pseudolinearis and P. dentata from Korea were crossed and the hybrid was cultured at different temperatures (5, 10, 15, 20 or $25^{\circ}C$), photon flux densities (10, 20, 40 or 80${\mu}$mol m$^{-2}$s$^{-1}$) under photoperiods (14L:10D and 10L:14D). In the hybrids, the conchocelis grew faster at 2$0^{\circ}C$ and 40$\mu$mol m$^{-2}$ s$^{-1}$ at $25^{\circ}C$ only, and were abundant, when cultured under 10L:14D. Foliose thalli of the hybrid grew rapidly at conditions of 10-2$0^{\circ}C$, 10L:14D and 15-2$0^{\circ}C$, 14L:10D but slowly at 5 and 2$0^{\circ}C$. No archeospores were observed any tested culture condition. Spermatangial and zygotosporangial sori were formed at the marginal portion o mature thallus. Zygotospores from the hybrid were released at 10-2$0^{\circ}C$ under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at 1$0^{\circ}C$ under 14L:10D. Neither monospores nor protothalli were produced from the conchocelis in culture.

• 한국수산과학회지
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• 제33권6호
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• pp.496-500
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• 2000

Partial fragments of nuclear 185 rDNAs from morphologically wide and narrow thalli of the seaweed Porphyra pseudolineazis were amplified and sequenced to compare their DNA homology. Both sequences of 311 base pairs showed $100{\%}$ identical each other. They showed $97.7{\%}$ similarity with a wild strain collected at Sodol in Kangwondo, and $99.4{\%}$ similarity with the GenBank accession number AB013185 of the Japanese P. pseudolinearis. Thus the morphological difference of wide and narrow blades might not be a classification criterion for the sub-species level of P. pseudolinearis.

• 생명과학회지
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• 제13권4호
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• pp.516-521
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• 2003

한국 동해안 특산종인 긴잎돌김의 원형질체 분리를 위한 적정한 조건을 구명하기 위한 연구가 이루어 졌다. 홍조류 긴잎돌김은 동해안에 있어 토착종이며 우점종중 하나이다. 원형질체는 2% agarase and 2% hemicellulase 의 효소를 이용하여 25mM Mes buffer, pH 6.0의 0.5M 솔비톨에서 분리되어 졌다. 분리되어진 원형질체는 PEG 8000에서 염색한 오카무라둥근돌김과 접합하였다.

• ALGAE
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• 제26권1호
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• pp.79-86
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• 2011

Physiological studies on the hybrid by crossing between two dioecious species, Porphyra pseudolinearis and P. dentata from Korea were conducted at constant temperatures (5, 10, 15, 20, and $25^{\circ}C$), at photon flux densities (10, 20, 40, and $80\;{\mu}mol\;m^{-2}s^{-1}$) under photoperiods (14 L : 10 D and 10 L : 14 D). In the hybrid, higher growth of conchocelis was observed at 20 and $40\;{\mu}mol\;m^{-2}s^{-1}$ under 14 L : 10 D. Conchosporangial branches were produced under $10-80\;{\mu}mol\;m^{-2}s^{-1}$ at only $25^{\circ}C$, and were abundant when the conchocelis was cultured under 10 L : 14 D. Foliose thalli of the hybrid grew well at the conditions of $10-20^{\circ}C$, 10 L : 14 D and $15-20^{\circ}C$, 14 L : 10 D. The foliose thalli grew very slowly at $5^{\circ}C$ and $30^{\circ}C$, respectively. No archeospores were observed at any culture conditions. Spermatangial and zygotosporangial sori were formed at the marginal portion of mature thallus. Zygotospores from the hybrid were released at $10-2^{\circ}C$ under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at $10^{\circ}C$ under 14 L : 10 D. Neither monospores nor protothalli were produced from the conchocelis in culture.

• 한국양식학회지
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• 제7권1호
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• pp.1-7
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• 1994

긴잎돌김 (Porphyra pscudolineris Ueda)의 엽상체와 배양 사상체에 미치는 온도와 광도의 영향을 구명하였다. 엽상체와 사상체의 총 광합성의 적정 온도는 각각 $25\~30^{\circ}C$, $20^{\circ}C$로 나타났다. 엽상체와 사상체에서 광도에 따른 광합성율은 공히 10,000 lux 이상에서 광합성율이 일정한 값에 도달하는 광포화 곡선을 나타내었다. 엽상체의 광 보상점은 2,100 lux, 사상체의 광 보강점은 900 lux였으며, 사상체의 광합성능은 엽상체의 5-10배에 해당하였다. 이로써 사상체는 낮은 광도하에서도 광합성을 수행할 수 있음을 알 수 있다.

• 생명과학회지
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• 제14권2호
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• pp.331-338
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• 2004

동해안 고유종인 긴잎돌김의 발생과 성숙, 생활사 등 생태에 관한 연구가 시행되었다. 긴잎돌김은 10월 초순에 발생하여 11월 말경에 성성숙이 시작되어 수정에 의해 과포자낭이 형성되고 과포자가 방출되었다. 3월 말경에 엽체가 소멸되었으며 이후 사상체, 각포자기를 거쳐 10월에 엽체가 발아하였다. 긴잎돌김의 특징으로 엽체는 피침형, 모근은 심장형이 대다수를 차지하였으며 거치상돌기가 없고 유성세대과정을 가지며 선명한 과포자낭무늬를 가진다. 암ㆍ수배우체에 있어 엽체와 엽폭비율은 성성숙기인 12월에 각각 5.6∼7.4 및 4.9∼7.3이였다. 1월에는 3.3∼4.8, 4.2∼4.8이였다. 개체수 변화는 10월 초순에 방형구(30${\times}$30 cm)내에 평균 5개체, 12월에는 238개체, 3월 중순에 15개체로 변화되었다. 성 분화가 시작되는 12월 초순에 암ㆍ수배우체 및 영양세포의 비율은 각각 31%, 47%, 22%였으며 완전 성숙된 12월 중순은 암ㆍ수배우체만 69.4% 및 30.6%였다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 1998년도 수산관련공동학술대회발표요지집
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• pp.151.1-152
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• 1998

• 생명과학회지
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• 제13권3호
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• pp.359-364
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• 2003

동해안에 있어 긴잎돌김은 우점종으로 조간대 상부지역에 서식하며 10월에 발생하여 3월에 소멸하였다. 10월에 영양세포로 육안 및 조직 절단에 의한 검색에서 성숙되지 않았다. 11월 중순경부터 육안으로는 암.수배우체가 구분되지 않았으나 조직 절단한 결과, 조정기와 조과기을 형성하고 있었다. 12월 초순부터 육안으로 암.수배우체가 확연히 구분되기 시작하였고, 2월경부터 성숙과 더불어 정자낭과 과포자낭의 방출로 점차 엽장의 길이가 줄어드는 추세를 보였다. 3월 초순경에는 색상이 퇴색되고 엽체 가변부에 녹아 내리는 현상이 나타나기 시작하여, 정자방출이 완료되었다. 12월 3일에 암컷의 평균 엽장은 113.0$\pm$6.8mm, 평균 엽폭은15.3$\pm$1.3mm로 나타났다. 수배우체의 평균엽장은 83.2$\pm$5.4mm, 평균엽폭은 16.8$\pm$1.9mm로 나타났다. 1월 28일 경에는 암배우체의 평균 엽장은 94.6$\pm$6.4mm로 엽폭은 29.1mm$\pm$5.1로 커졌다. 수배우체의 경우에 평균 엽장이 107.8$\pm$7.3mm, 평균 엽폭은 25.9$\pm$0.9mm로 엽장과 엽폭이 반비례되는 현상이 나타났다.

• 한국식품영양과학회지
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• 제27권6호
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• pp.1166-1172
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• 1998

A method for the isolation and purification of DNA from a red algae, Porphyra was innovated. The innovation of the method consists mainly of three steps that include sodium acetate treatment, chloroform extraction, and 0.2 volume isopropanol precipitation step. The sodium acetate treatment was designed to remove polysaccharide contamination, and the isopropanol step to remove proteins and salts contaminents. Genomic DNA,s of several species(for example, P. tenera, P. yezoensis, P. seriata, and P. pseudolinearis) was successfully isolated by the innovated method. The amount of DNA purified from one g of sample material with the innovated method was 53 g in average. The resulting DNA was characterized to include high molecular weight and showed no nuclease activity. The DNA was pure enough to be digested directly by various restriction enzymes without any difficulties. Porphyra DNA was pure enough and adequate for amplification reaction through the polymerase chain reaction (small nuclear rDNA PCR amplification).

• ALGAE
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• 제18권4호
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• pp.321-331
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• 2003

Cryopreservation of sporothalli of the red alga Porphyra (P. seriata, P. yezoensis, P. tenera, P. pseudolinearis and P. dentata) was performed by the two-step cooling method in liquid nitrogen. The algal samples were suspended in various cryoprotective solutions, and slowly cooled to -40$^{\circ}C$ in 4 hours using a programmed freezer. After the first slow cooling the suspensions with cryoprotectants were immediately immersed in liquid nitrogen. The suspension from the programmed freezer was thawed quickly by agitation of the vial in a water bath at 40°C. When ice in the suspension of cryogenic vial was mostly melted, the vial was transferred to an ice bath for complete melting of the residual ice. The cryoprotectants in the vial were washed off by gradual dilution with seawater. The viability of the cell was assessed with neutral red staining. The viability of Porphyra samples ranged 54.6-70.9% when the mixed suspension of 10% dimethylsulfoxide and 0.5 M sorbitol in 50% seawater used as a cryoprotectant.