• BMB Reports
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• v.54 no.10
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• pp.505-515
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• 2021

Human pluripotent stem cells (hPSCs) include human embryonic stem cells (hESCs) derived from blastocysts and human induced pluripotent stem cells (hiPSCs) generated from somatic cell reprogramming. Due to their self-renewal ability and pluripotent differentiation potential, hPSCs serve as an excellent experimental platform for human development, disease modeling, drug screening, and cell therapy. Traditionally, hPSCs were considered to form a homogenous population. However, recent advances in single cell technologies revealed a high degree of variability between individual cells within a hPSC population. Different types of heterogeneity can arise by genetic and epigenetic abnormalities associated with long-term in vitro culture and somatic cell reprogramming. These variations initially appear in a rare population of cells. However, some cancer-related variations can confer growth advantages to the affected cells and alter cellular phenotypes, which raises significant concerns in hPSC applications. In contrast, other types of heterogeneity are related to intrinsic features of hPSCs such as asynchronous cell cycle and spatial asymmetry in cell adhesion. A growing body of evidence suggests that hPSCs exploit the intrinsic heterogeneity to produce multiple lineages during differentiation. This idea offers a new concept of pluripotency with single cell heterogeneity as an integral element. Collectively, single cell heterogeneity is Janus-faced in hPSC function and application. Harmful heterogeneity has to be minimized by improving culture conditions and screening methods. However, other heterogeneity that is integral for pluripotency can be utilized to control hPSC proliferation and differentiation.

• The Korean Journal of Ecology
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• v.18 no.4
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• pp.512-523
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• 1995

The central theme of Habital heterogeneity is to provide animals with habital complexity or structural diversity and to allow resource partitioning among individuals. In turn, the leads to population stability because prey can escape more easily with more hiding places causing less population fluctuation. Species diversity is characterized due to more potential niches both horizontally and verticall. Empirically, in homogeneous habitats population was less abundant, reproduction and survival were lower, spacing behavior, competition and dispersal were higher than in heterogeneous habitats. The results imply that diversity and conservation of species can be maintained through providing heterogeneous habitats.

• The Korean Journal of Ecology
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• v.18 no.4
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• pp.513-513
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• 1995

The central theme of Habital heterogeneity is to provide animals with habital complexity or structural diversity and to allow resource partitioning among individuals. In turn, the leads to population stability because prey can escape more easily with more hiding places causing less population fluctuation. Species diversity is characterized due to more potential niches both horizontally and verticall. Empirically, in homogeneous habitats population was less abundant, reproduction and survival were lower, spacing behavior, competition and dispersal were higher than in heterogeneous habitats. The results imply that diversity and conservation of species can be maintained through providing heterogeneous habitats.

• The Korean Journal of Ecology
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• v.24 no.6
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• pp.353-357
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• 2001

Differential response of genotypes to temporal environmental heterogeneity may contribute to the long-term persistence of these genotypes within a population. In this experiment, we experimentally tested whether groups by season interactions for germination and seedling growth can explain genetic variability within the population. To determine whether seeds collected during the four seasons respond differentially to temperature treatments, two-way ANOVA was performed. This study indicates that seasonal environments have large effects on demography. Groups within populations respond differentially to seasonal environments by influencing population growth that may in turn influence community composition. Most importantly, the study showed that temporal heterogeneity in the environment might functions as a mechanism that maintains within-population genetic diversity.

• Molecules and Cells
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• v.42 no.3
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• pp.189-199
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• 2019

Cell-to-cell variability in gene expression exists even in a homogeneous population of cells. Dissecting such cellular heterogeneity within a biological system is a prerequisite for understanding how a biological system is developed, homeostatically regulated, and responds to external perturbations. Single-cell RNA sequencing (scRNA-seq) allows the quantitative and unbiased characterization of cellular heterogeneity by providing genome-wide molecular profiles from tens of thousands of individual cells. A major question in analyzing scRNA-seq data is how to account for the observed cell-to-cell variability. In this review, we provide an overview of scRNA-seq protocols, computational approaches for dissecting cellular heterogeneity, and future directions of single-cell transcriptomic analysis.

• Korean Journal of Environmental Biology
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• v.21 no.4
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• pp.337-341
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• 2003

Habitat heterogeneity can enhance biological diversity by providing variation in structural diversity. This paper reviewed heterogeneous habitat serves as a population stability and superior demographic performance (e.g., high density, survivorship, reproductive rate) can be observed compared with organisms with inferior demographic performance. The idea of habitat variation has been further developed in modelling. Furthermore the size and configuration (distribution) of a patch (of a particular habitat type) become effective for the stability of population through hiding places and food resources. Species diversity is related to habitat complexity that provides structural diversity to ground -dwelling organisms. Finally coarse woody debris can enhance habitat complexity thus stabilizing population fluctuation and increasing survivorship.

• Genomics & Informatics
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• v.17 no.1
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• pp.3.1-3.4
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• 2019

Intratumor heterogeneity within a single tumor mass is one of the hallmarks of malignancy and has been reported in various tumor types. The molecular characterization of intratumor heterogeneity in breast cancer is a significant challenge for effective treatment. Using single-cell RNA sequencing (RNA-seq) data from a public resource, an ERBB pathway activated triple-negative cell population was identified. The differential expression of three subtyping marker genes (ERBB2, ESR1, and PGR) was not changed in the bulk RNA-seq data, but the single-cell transcriptomes showed intratumor heterogeneity. This result shows that ERBB signaling is activated using an indirect route and that the molecular subtype is changed on a single-cell level. Our data propose a different view on breast cancer subtypes, clarifying much confusion in this field and contributing to precision medicine.

• The Korean Journal of Applied Statistics
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• v.30 no.2
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• pp.301-309
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• 2017

It is often possible to test for differences in population means when two or more samples are extracted from each N population. However, it is not possible to test for the mean difference if one sample is extracted from each population since a sample mean does not exist. But, by dividing a group of samples extracted one by one into two groups and generating a sample mean, we can identify a heterogeneity that may exist within the group by comparing the differences of the groups' mean. Therefore, we propose a minimum combination t-test method that can test the mean difference by the number of combinations that can be divided into two groups. In this paper, we proposed a method to test differences between means to check heterogeneity in a group of extracted samples. We verified the performance of the method by simulation study and obtained the results through real data analysis.

• The Journal of the Korean Society for Microbiology
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• v.21 no.2
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• pp.191-204
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• 1986

Leptospira isolated from the patients with so called epidemic pulmonary hemorrhagic fever were comparatively studied with standard strains obtained from ATCC. The specific aim of this study was to clarify the morphologic heterogeneity of the isolates, i. e., coexistence of spiral forms in both handness, right and left, rod and spherical forms in their population by comparing them with those of the ATCC strains. No differences between our strains and ATCC strains were noticed in their growth characteristics, responses to the culture media, temperature, antibiotics and antifungal agents. Furthermore, the morphologic heterogeneity had been repeatedly observed even in cultures of standard ATCC strains, which had been noticed in the cultures of bacteria isolated in this laboratory. The serologic analysis of our isolates demonstrated that the bacteria reacted with L. icterohaemorrhagiae and L. australis regardless their differences in time of isolation(1984, 1985). Thus, it was concluded that the spiral bacteria isolated in this laboratory are Leptospira of a new serovar which still remained to be determined. And the previous reports on the morphology of the Leptospira, which described that the Leprospira population has only spiral forms with right. handed coils might be reconsidered.

• International Journal of Advanced Culture Technology
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• v.9 no.2
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• pp.32-38
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• 2021

The purpose of this study is to examine the spatial characteristics of violent and burglary crimes in South Korea. Violent crimes and burglary crimes depend on a spatial setting with good conditions for their criminal purposes. This study defines population density, racial heterogeneity, types of houses, and density of commercial facilities as variables of place affecting crime in cities and counties. The study collects data from 229 cities in Korea to analyze the effect of spatial characteristics on crime. We conduct additional analyses to meet the statistical requisites of the spatial econometrics model using the open-source software R and GeoDa 1.12.1.129. From the analytical result, population density, racial heterogeneity, apartments, and commercial areas relate to crime occurrence. We suggest the implication of the theoretical and practical contributions to the relationship between place and crime.