• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.218-218
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• 2022

Granules-bound starch synthase II (GBSSII), one of the isoforms of granule-bound starch synthase (GBSS), is responsible for amylose synthesis by expressing in non-storage tissues such as leaf, stem, root, and pericarp. Up to date, little is known about this gene functions and basic knowledge of heritable characteristics of this gene, GBSSII. We identified functional haplotypes and performed evolutionary analyses on the GBSSII using 374 rice accessions (320 Korean bred and 54 wild) based on the classified groups. A total of 14 haplotypes were found, and almost all haplotypes (13) were functional, carrying 19 non-synonymous SNPs in two exons (exons 1 and 2). The lowest nucleotide diversity was detected in Tropical japonica (0.00145), while the highest pi-value was in Aus (0.01081), illustrating the signal of this gene evolution. The highest Tajima's D value in Aus (1.6380) indicates GBSSII gene domestication signature under balancing selection, while the lowest Tajima's D value in Temperate japonica (-0.8243) highlights that they were under positive selection, which may be purified due to the excess of rare alleles. The highest genetic differentiation was observed between Tropical japonica and aroma (F_ST = 0.921928). In contrast, the highest interbreed level was detected in Aus-admixture (F_ST = -0.20157). The genetic relatedness between and or among the wild and cultivated subpopulations was revealed through PCA, population structure, and phylogenetic analyses.

• 정보과학회 논문지
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• 제41권12호
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• pp.1090-1098
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• 2014

이 연구는 단봉 전역 최적화 성능이 개선된 적응적 코시 분포 차분 진화 알고리즘을 제안한다. 기존 적응적 코시 분포 차분 진화 알고리즘은(ACDE) 개체의 다양성을 보장하여 다봉 전역 최적화 문제에 우수한 "DE/rand/1" 돌연변이 전략을 사용했다. 그러나 이 돌연변이 전략은 수렴 속도가 느려 단봉 전역 최적화 문제에 단점이 있다. 제안 알고리즘은 "DE/rand/1" 돌연변이 전략 대신 수렴 속도가 빠른 "DE/current-to-best/1" 돌연변이 전략을 사용했다. 이때, 개체의 다양성이 부족하여 발생할 수 있는 지역 최적해로의 수렴을 방지하기 위해서 매개변수 초기화 연산이 추가됐다. 매개변수 초기화 연산은 특정세대를 주기로 실행되거나 또는 선택 연산에서 모든 개체가 진화에 실패하는 경우 실행된다. 매개변수 초기화 연산은 각 개체들의 매개변수에 탐험적 특성이 높은 값을 할당하여 넓은 공간을 탐색할 수 있도록 보장한다. 성능 평가 결과, 개선된 적응적 코시 분포 차분 진화 알고리즘이 최신 차분 진화 알고리즘들에 비해 특히, 단봉 전역 최적화 문제에서 성능이 개선됨을 확인했다.

• 한국기후변화학회지
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• 제7권4호
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• pp.465-476
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• 2016

This study was conducted to elucidate the ecological characteristics of sub-alpine coniferous forest on Banyabong, Mt. Jiri. Soil pH was 4.20. The contents of organic matter, total N, available P and CEC were 14.15%, 0.43%, 10.1 ppm and $17.17cmol^+/kg$, respectively. Sub-alpine coniferous forest had a relatively soil moisture content with 22.9%. According to the results of importance value analysis, Picea jezoensis (42.7) and Abies koreana (34.0) for tree layer, Rhododendron schlippenbachii (25.5) for subtree layer and Tripterygium regelii (18.4) and Rhododendron mucronulatum (16.6) for shrub layer were high, respectively. Shannon's diversity ranged from 0.3560 to 1.050. Evenness and dominance ranged from 0.398 to 0.733 and 0.267 to 0.601, respectively. A. koreana population had a reverse J-shaped DBH distribution, while P. jezoensis population had a normal DBH distribution. Height growth according to DBH of A. koreana was higher than that of P. jezoensis, which indicate than A. koreana have more effective water use efficiency than P. jezoensis. Annual mean tree ring growth of P. jezoensis and A. koreana showed up 1.792, 1.652, 1.389 mm/yr and 2.081, 1.921, 1.576 mm/yr for small, middle and large diameter tree, respectively.

• Fisheries and Aquatic Sciences
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• 제27권3호
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• pp.145-158
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• 2024

Life history characteristics, habitat landscape, and historical events are believed to have shaped the patterns of genetic variation in many taxa. The bony lip barb, Osteohilus vittatus, represent a potamodromous fish that complete all life cycle in freshwater and is widely distributed in Southeast Asia. It usually lives in small rivers and other freshwater habitats, and movement between habitats for either food or reproduction has been typical. These life history characteristics may promote gene flow, leading to less structured populations. However, many freshwater habitats are fragmented, which restricts gene flow. We investigate how this interplay has shaped patterns of genetic variation and phylogeographic structure within this species in the Sundaland, a biodiversity hotspot with a complex geological history, using mitochondrial cytochrome oxidase I (mtCOI) as a genetic marker. Forty-six mtCOI sequences of 506 bp long were collected from ten localities, eight geographically isolated and two connected. The sequences were used for population genetic and phylogeographic analyses. Our results showed a low genetic diversity within populations but high between populations. There was a deep phylogeographic structure among geographically isolated populations but a lack of such structure in the connected habitats. Among geographically isolated populations, sequence divergence was revealed, ranging from 1.8% between Java and Sumatra populations to 12.2% between Malaysia and Vietnam. An indication of structuring was also observed among localities that are geographically closer but without connectivity. We conclude that despite high dispersal capacity, the joint effects of historical events, long-term geographic isolation associated with sea level oscillation during the Pleistocene, and restricted gene flow related to lack of habitat connectivity have shaped the phylogeographic structure within the O. vittatus over the Sundaland.

• 한국균학회지
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• 제27권5호
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• pp.328-336
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• 1999

Isozyme comparisons of mycelial extracts from Pleurotus ostreatus were undertaken using isoelectric focusing. Enzyme isozyme patterns were Used to describe the extent of geographical diversity and degree of intraspecific variation in these extracts. A total of 77 bands were resolved from six different enzymes. Cluster analyses were performed using the zymograms for esterase (EST), glucose phosphate isomerase (GPI), leucine aminopeptidase (LAP), malate dehydrogenase(MDH), peroxidase (POX), and phosphoglucomutase (pGM). EST gave multiple banding patterns, while less variability was observed for GPI, MDH, and PGM. Cluster analyses demonstrated that strains of P. ostreatus from geographically different origins are genetically divergent, supporting the idea that there is little or no gene flow between these geographically distant population groups.

• Journal of Plant Biotechnology
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• 제4권2호
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• pp.45-52
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• 2002

A lot of SSRs (simple sequence repeats) in peach and pear from enriched genomic libraries and in peach from a cDNA library were developed. These SSRs were applied to other related species, giving phenograms of 52 Prunes and 60 pear accessions. Apple SSRs could also be successfully used in Pyrus spp. Thirteen morphological traits were characterized on the basis of the linkage map obtained from an $F_2$ population of peach. This map was compiled with those morphological markers and 83 DNA markers, including SSR markers used as anchor loci, to compare different peach maps. Molecular markers tightly linked to new root-knot nematode resistance genes were also found. A linkage map including disease related genes, pear scab resistance and black spot susceptibility, in the Japanese pear Kinchaku were constructed using 118 RAPD markers. Another linkage map, of the European pear Bartlett, was also constructed with 226 markers, including 49 SSRs from pear, apple, peach and cherry. Maps of other Japanese pear cultivars, i.e., Kousui and Housui, were also constructed. These maps were the first results of pear species.

• Fisheries and Aquatic Sciences
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• 제12권1호
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• pp.35-43
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• 2009

Sturgeons and paddlefishes are frequently referred to as 'living fossils' among the actionpterygian lineage. They are increasingly facing threats to their existence because of various anthropogenic pressures. In this study, we present the complete mitogenome sequence of the Russian sturgeon Acipenser gueldenstaedtii (Acipenseriformes; Acipenseridae). The mitogenome showed highly homogeneous molecular features compared to previously known vertebrate mitogenomes. Phylogenetic tree inferred from concatenated protein-coding and tRNA genes unambiguously revealed the monophyly of A. gueldenstaedtii, Acipenser stellatus, and Huso huso. Genetic information of the endangered A. gueldenstaedtii will provide baseline data needed to develop molecular markers for stock identification and assessment of population diversity and also to develop future conservation strategies.

• Journal of information and communication convergence engineering
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• 제3권1호
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• pp.43-48
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• 2005

In this paper, the adaptive fuzzy logic controller(AFLC) is proposed, which uses real-coding genetic algorithm showing a good performance on convergence velocity and diversity of population among evolutionary computations. The effectiveness of the proposed AFLC was demonstrated by computer simulation for speed control system of AC servo motor. As a result of simulation for the AC servo motor, it is shown the proposed AFLC has the better performance on overshoot, settling time and rising time than the PI controller which is used when tuning AFLC.

• The Plant Pathology Journal
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• 제32권1호
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• pp.47-52
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• 2016

Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between Arich sequence and ${\beta}C1$ gene and insertion of 27 nt in the middle of ${\beta}C1$ ORF. This study may help in investigating molecular basis of resistance in G. arboreum.

• Animal Systematics, Evolution and Diversity
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• 제28권1호
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• pp.48-53
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• 2012

The tiger population that once inhabited the Korean peninsula was initially considered a unique subspecies (Panthera tigris coreensis), distinct from the Amur tiger of the Russian Far East (P. t. altaica). However, in the following decades, the population of P. t. coreensis was classified as P. t. altaica and hence forth the two populations have been considered the same subspecies. From an ecological point of view, the classification of the Korean tiger population as P. t. altaica is a plausible conclusion. Historically, there were no major dispersal barriers between the Korean peninsula and the habitat of Amur tigers in Far Eastern Russia and northeastern China that might prevent gene flow, especially for a large carnivore with long-distance dispersal abilities. However, there has yet to be a genetic study to confirm the subspecific status of the Korean tiger. Bone samples from four tigers originally caught in the Korean peninsula were collected from two museums in Japan and the United States. Eight mitochondrial gene fragments were sequenced and compared to previously published tiger subspecies' mtDNA sequences to assess the phylogenetic relationship of the Korean tiger. Three individuals shared an identical haplotype with the Amur tigers. One specimen grouped with Malayan tigers, perhaps due to misidentification or mislabeling of the sample. Our results support the conclusion that the Korean tiger should be classified as P. t. altaica, which has important implications for the conservation and reintroduction of Korean tigers.