• Title/Summary/Keyword: Poplar Forest

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Gene Manipulation of Pin 2(Proteinase Inhibitor II) to the Cottonwood Leaf Beetle(Coleoptera : Chrysomelidae) in Transgenic Poplar(Populus deltodies × P. nigra) (형질전환(形質轉換)된 포플러의 딱정벌레에 대한 저항성(抵抗性) 유전자(遺傳子)(Proteinase Inhibitor II) 발현(發現))

  • Kang, Hoduck
    • Journal of Korean Society of Forest Science
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    • v.86 no.4
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    • pp.407-414
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    • 1997
  • The resistance of a non-transgenic poplar clone, 'Ogy' and three transgenic poplar lines to the cottonwood leaf beetle, Chrysomela scripta F., was evaluated by in vitro feeding. The lines were transformed with neomycin phosphotransferase II(NPT II) as a selectable marker, proteinase inhibitor II(pin2) as a resistance gene, and CaMV 35S as a promoter. An efficient method of sterilizing the beetle eggs and introducing them into plant tissue cultures was developed. The resistance of the transgenic lines was investigated in terms of effects tin leaf area consumed, insect weight, insect developmental stages, and plantlet root dry weight after feeding. Also, leaf area consumed was examined by leaf age as measured through leaf plastochron index(LPI). The leaf area consumed and insect weight were highly significant between transformants and control, and insect development in vitro was significant among the transgenic lines. Larval infestation was the most severe around LPI 4 to 5 which were young leaves. The system provided a quick, highly controlled method to screen developing transgenic plantlets directly.

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Application of in vitro ovary culture for cottonwood (Populus deltoides) breeding

  • Kang, Ho-Duck;Lee, Min-Soon
    • Plant Resources
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    • v.7 no.2
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    • pp.87-92
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    • 2004
  • Five different poplar hybrids were tested for rescuing embryo to elongate in vitro plantiets after hybridization. Ovaries and ovules were cultured on Woody Plant Medium (WPM) supplemented with cytokinins, 6-benzylamine (BA) and zeatin. Multiple shoots were initiated from half section of capsule with immature embryos after 21 days from pollination and tiny shoots were formed after the expansion of cotyledons in ovule cultures. Germinating response was better in intraspecific hybrids $(6.53\pm1.66)$ than interspecific crosses $(0.93\pm0.54)$ from half section of capsules on WPM medium. In general, zeatin was better than BA in inducing multiple shoots from isolated ovules. The highest average number $(19.40\pm4.53)$ of shoots was produced from immature ovules of 21 days post-pollination of WPM medium supplemented with 5.0 mg/L zeatin. The highest percentage of germination was 93% from the half section of in vitro ovary cultures. Soil acclimation was successfully conducted in cell tray containing artificially mixed soil with 96% survival rate.

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Comparative Wood Anatomy of Stem and Root in Korean-grown Yellow-poplar (Liriodendron tulipipfera L.)

  • Lee, Mi-Rim;Eom, Young-Geun
    • Journal of the Korean Wood Science and Technology
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    • v.39 no.5
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    • pp.406-419
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    • 2011
  • This study was carried out to offer basic information on the wood anatomy of domestic yellow-poplar (Liriodendron tulipifera L.), a new plantation species selected by Korea Forest Service as one of the promising hardwood and bioenergy sources of the future, through comparison of stem wood with root wood in the qualitative and quantitative features. In the qualitative anatomical features, growth rings were distinct in stem wood but relatively less distinct in root wood. And stem wood appeared to have pores in radial multiples of 2 to 5, sometimes clusters but root wood to have pores in radial multiples of 2 to 3, rarely clusters. And numbers of bars in scalariform perforation plates were somewhat numerous in vessel elements of root wood than in those of stem wood. Interestingly, on the other hand, more extraneous materials in the wood rays of tap root than in those of lateral root and stem were confirmed in the chemical composition analyses. In the quantitative anatomical features, pore densities were significantly greater but vessel elements were considerably narrower in stem wood than in root wood. Vessel elements and wood fibers of root wood were considerably longer than those of stem wood. Rays were somewhat more numerous in stem wood than in root wood, and only ray heights of stem wood were more or less greater in cell numbers but both ray heights and widths of stem wood were lower in dimension than those of root wood. The anatomical differences between stem wood and root wood were thought to be associated with different growth environments between the stem above ground and the root below ground.

Optimization of The Organosolv Pretreatment of Yellow Poplar for Bioethanol Production by Response Surface Methodology

  • Kim, Ho-Yong;Hong, Chang-Young;Kim, Seon-Hong;Yeo, Hwanmyeong;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.5
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    • pp.600-612
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    • 2015
  • We investigated the optimization of the organosolv pretreatment of yellow poplar for bioethanol production. Response surface methodology was used to determine the optimal conditions of three independent variables (reaction temperature, reaction time, and sulfuric acid (SA) concentration). Reaction temperature is the most significant variable in the degradation of xylan and lignin in the presence of an acid catalyst, and ethanol production increased with a decrease in the lignin content. The highest ethanol concentration ($42.80g/{\ell}$) and theoretical ethanol yield (98.76%) were obtained at $152^{\circ}C$ (2.5 bar) with 1.6% SA for 16 min. However, because of excessive degradation of the raw material, the overall ethanol yield was less than under other pretreatment conditions which has approximately 50% of WIS recovery rate after pretreatment. The optimal conditions for the maximum overall ethanol yield ($146^{\circ}C$ with 1.22% SA for 15.9 min) were determined with a predicted yield of 17.11%, and the experimental values were very close (17.15%). Therefore, the quadratic model is reliable.

Extractives of the Wood of Black Locust and the Bark of Poplar as Decay-Resistant Hardwood Tree Species (내후성 활엽수종인 아까시나무 목질부와 현사시나무 수피의 추출성분)

  • Bae, Young-Soo;Ham, Yeon-Ho
    • Journal of the Korean Wood Science and Technology
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    • v.28 no.3
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    • pp.52-61
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    • 2000
  • Black locust(Robinia pseudoacacia) and poplar(Populus alba ${\times}$ glandulosa) trees were collected, extracted with acetone-$H_2O$(7:3, v/v) after drying, fractionated with hexane, chloroform and ethylacetate, and freeze dried to get some brown powder. Each fraction of the powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluting solvents. The wood extractives of black locust contained (+)-leucorobinetinidin as flavan, robtin as flavanone and dihydrorobinetin as flavanonol, and robinetin as flavonol. The poplar bark extractives contained various kinds of phenolic compounds : (+)-catechin as flavan, naringeoin, eriodictyol, sakuranetin, aromadendrin and taxifolin as flavanonol, salireposide as salicin derivative, and minor compounds such as aesculin and p-coumaric acid. However, aesculin has not been reported as a constituent of the poplar bark in Korea yet. NMR and FAB-MS analyses were done to elucidate the structures of isolated phenolic constituents.

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Feasibility of Manufacturing Desk and Chair with Curved Veneer Lamination (단판 적층성형 학생용 책상.의자의 제조적성)

  • Suh, Jin-Suk;Park, Jong-Young;Han, Ki-Man
    • Journal of the Korea Furniture Society
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    • v.16 no.2 s.30
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    • pp.59-65
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    • 2005
  • As physical condition of students improves, there is a need to develop human body-friendly desk and chair for students. In this study, desks and chairs were manufactured with curved veneer lamination under high frequency heating and pressing, using ten wood species such as Japanese red pine, Korean pine, pitch pine, Japanese larch, yellow poplar, black locust, oak, radiata pine, beech, and birch. The performance of these products were evaluated. The results obtained were summarized as follows; With high frequency heating, the turned lamination of veneers with full size sheet ($3{\times}6\;feet$) prepared by rotary lathe peeling was successfully applied for making the members of desk top, leg frames of desk and chair. Bending strengths of desk tops were relatively greater for yellow poplar, black locust and red pine, which were similar to those of beech and birch. Bending strengths of desk legs were classified into greater species group (red pine, yellow poplar, larch) and lower species group (radiata pine, Korean pine, pitch pine). Compressive strengths of chair legs in parallel direction to the lamination were greater in black locust and larch. On the other hand, differences between outer and inner gap at the top and drawer bottom of desk top were rather larger for the laminations of birch and beech, and less for those of yellow poplar and pitch pine, showing greater stability of open drawer space. In results, yellow poplar, larch, pitch pine and red pine showed good appearance and strength properties at the curved veneer lamination. Accordingly, it was believed that these domestic woods were able to substitute for birch which was being imported for the use of veneer-laminates type furniture.

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Color Control and Durability Improvement of Yellow Poplar (Liriodendron tulipifera) by Heat Treatments (열처리에 의한 백합나무 재색 제어와 내부후성 제고)

  • Yoon, Kyung-Jin;Eom, Chang-Deuk;Park, Jun-Ho;Kim, Ho-Yong;Choi, In-Gyu;Lee, Jun-Jae;Yeo, Hwanmyeong
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.6
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    • pp.487-496
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    • 2009
  • The sapwood of yellow poplar is very bright while its heartwood is usually greenish which changes to dark brown with weathering. This difference in color value between sapwood and heartwood causes difficulty in using yellow poplar as higher value added materials such as interior finish and furniture part. In this study, hot-water treatment, vacuum-heat treatment and oven-heat treatment were carried out to reduce the difference in color value between heartwood and bright sap wood and to increase durability. FT-IR analysis, contact angle measurement and decay test were carried out to find out the mechanism of functional group change and the increment of durability by heat treatment. The result of decrement ratio of color difference were 45.7% by hot-water treatment, 26.8% by vacuum-heat treatment, and 60.2%, 87.8%, and 88.8% by $180^{\circ}C$, $200^{\circ}C$ and $220^{\circ}C$ oven-heat treatments respectively. Furthermore, it has been found that oven-heat treatment causes decrement of mass loss by decay in this study. It is suggested that oven-heat treatment could be environmentally friendly preservative treatment without chemicals.