• Journal of Microbiology and Biotechnology
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• v.33 no.12
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• pp.1692-1697
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• 2023

Staphylococcus aureus integrated with mecA gene, which codes for penicillin-binding protein 2a, is resistant to all penicillins and other beta-lactam antibiotics, resulting in poor treatment expectations in skin and soft tissue infections. The development of a simple, sensitive and portable biosensor for mecA gene analysis in S. aureus is urgently needed. Herein, we propose a dual-toehold-probe (sensing probe)-mediated exonuclease-III (Exo-III)-assisted signal recycling for portable detection of the mecA gene in S. aureus. When the target mecA gene is present, it hybridizes with the sensing probe, initiating Exo III-assisted dual signal recycles, which in turn release numerous "3" sequences. The released "3" sequences initiate catalytic hairpin amplification, resulting in the fixation of a sucrase-labeled H2 probe on the surface of magnetic beads (MBs). After magnet-based enrichment of an MB-H1-H2-sucrase complex and removal of a liquid supernatant containing free sucrase, the complex is then used to catalyze sucrose to glucose, which can be quantitatively detected by a personal glucose meter. With a limit of detection of 4.36 fM for mecA gene, the developed strategy exhibits high sensitivity. In addition, good selectivity and anti-interference capability were also attained with this method, making it promising for antibiotic tolerance analysis at the point-of-care.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.2
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• pp.455-462
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• 2004

Prostate cancer is a leading cause of cancer death in American men and evidences point to significant life style/diet components as risk factors for its development or prevention. Two large cohort studies have identified the consumption of tomatoes or high Plasma levels of Iycopene as associated with reduced risk. A number of other substances such as quercetin, phytoene, phytofluene, cyclolycopene, salicylates and tomatine in tomato besides lycopene could have anticancer activity and may be acting synergistically with lycopene. Lycopene at almost physiologically feasible concentrations, reduces cell viability by cell cycle arrest and apoptosis and modulates the cyclin pathways as well as increasing intercellular communication. However, it is not clear whether lycopene or its oxidation products are more bioactive. Tomato product supplementation results in plasma accumulation of phytoene, Phytofluene, the lycopene oxidation product, and cyclolycopene at significant concentrations and lycopene supplementation, either as a tomato product or as beadlets, results in maximal mean plasma lycopene concentrations of ∼ 1 $\mu$M which is at the lower limit of its activity in cell culture. Rats and mice are poor accumulators of lycopene and other carotenoids making them poor models for the study of cancer prevention and control. Of the 19 animal studies for various cancer sites, lycopene showed a positive effect in 10 studies but negative in 2 prostate cancer studies. In vivo prevention of leukocyte DNA damage in humans has been mostly studied using tomato product supplementation but lycopene supplementation appeared to reduce oxidative DNA damage as well as tomato product supplementation. Lycopene appears to be bioactive in intefering with carcinogenesis but the actions of phytoene, phytofluene or cyclolycopene cannot be ruled out since these compounds were present in most of the lycopene material used for these studies. Although lycopene remains as a promising agent, especially for cancer control, exploring interactions with other tomato phytochemicals and with current prostate cancer therapies should be encouraged.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.6
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• pp.706-714
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• 2020

Currently, 90 % of the world's population breathes air with a fine dust content exceeding the World Health Organization's annual average exposure limit (10 ㎍/㎥). Global efforts have been devoted toward reducing secondary pollutants and ultra-fine dust through regulations on nitrogen oxides released over land and sea. Domestic efforts have also aimed at creating clean marine environments by reducing sulfur emissions, which are the primary cause of dust accumulation in ships, through developing and distributing environment-friendly ships. Among the technologies for reducing harmful emissions from diesel engines, electrostatic precipitator offer several advantages such as a low pressure loss, high dust collection efficiency, and NOx removal and maintenance. This study aims to increase the durability of a ship by improving equipment quality through failure mode effects analysis for the preventive maintenance of an electrostatic precipitator that was developed for reducing fine dust particles emitted from the 2,427 kW marine diesel engines in ships with a gross tonnage of 999 tons. With regard to risk priority, failure mode 241 (poor dust capture efficiency) was the highest, with an RPN of 180. It was necessary to determine the high-risk failure mode in the collecting electrode and manage it intensively. This was caused by clearance defects, owing to vibrations and consequent pin loosening. Given that pin loosening is mainly caused by vibrations generated in the hull or equipment, it is necessary to manage the position of pin loosening.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.4
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• pp.195-203
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• 1974

The individual production rate of Plecoglossus altivelis was studied from the 18th of June to the 25th of September (99 days) 1973, when the fish grow best at Gangjeong Stream, Jeju Do, as one of the ways to find out the reasons why the size of P. altivelis of Jeju Island is smaller than that of other places. The results obtained are as follows : 1) The indivudual production rate of the fish in the Gangjeong Stream during the summer 1973, was estimated as shown in Table 6. 2) The individual production rate of the fish and standing crop of algae on stones in the Gang-jeon Stream were no more than $7.2\%$ and $6.2\%$ respectively, when compared with those of the Ukawa River (which is located in Japan), thus the individual production rate of the fish at Gang-jeong Stream being very poor. 3) Population density was 5.2 individuals average per square meter during the research period. This is beyond the estimated density limit, i. e., 4.8 individuals per square meter, for the standing crop of algae on stones. 4) The period that the individual production rate of the fish was the most brisk during the summer in this stream was from August to September in 1973. 5) The poor individual production rate is primarily regarded due to insufficient gross ingestion. The reasons seemed to be insufficeient productivity of algae on the bottom stones, over-population density of the fish, and the lowered activity of ingestion due to low water temperature in daytime as compared with other places. Thus, the production rate of Plecoglossus altivelis in the Gangjeong Stream during the summer was greatly influenced by these factors.

• Tunnel and Underground Space
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• v.22 no.3
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• pp.163-172
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• 2012

As more diesel engines have been employed in underground limestone mines with large cross section, underground space environment is worsened by diesel exhausts and heat flow. This paper aims for the ultimate goal to optimize the work place environment through assuring the optimal required ventilation rate based on the analysis of the airflow, diesel exhaust gas concentrations and the effects of mechanization and deepening working face on temperature and humidity. Due to the insufficient capacity of the main exhaust fan and poor airway management, stagnant airflows were observed at various locations, while the flow direction was reversed instantly with passing diesel equipment and the flow reversal was also made by the seasonal variation of the outside surface weather. During the loading operation, CO concentration measurements were found to be frequently higher than the threshold limit of 50 ppm, and most of the $NO_2$ measurements during drilling and loading operations shows even more serious levels surpassing the permissible limit of 3 ppm. The actual ventilation quantity was considerably less than the required quantity estimated by the mine health and safety law, and this shortage problem was less serious in colder winter showing more effectiveness of the natural ventilation.

• Convergence Security Journal
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• v.13 no.2
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• pp.3-13
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• 2013

School violence that have occurred recently, showing a tendency to collectivization and diverse types of violence, as well as the quality and increase the damage levels. School violence in the home, school, and social factors, but is caused by the lack of awareness about the violence and the reporting of consciousness due to poor acts of violence indirectly assisted. The only place violence has caused the school to establish the legal and institutional arrangements in order to minimize school violence have no choice but to limit its effectiveness is negligible. The problem of school violence in connection with the problem of juvenile crime prevention and control, and punishment should be made of the complex and layered. Operation and School police system, School safety keeper system, school sheriff system since 2005, each municipality in order to minimize school violence, but have no practical help to limit the visible and symbolic effects. Nonexistent professional staff of the institution or school safety monitors emphasis on monitoring the physical state of the system in the form of 'guards' departure inherently have limitations. Also, to prevent criminal acts or violence in the state is not given special privileges and the appropriate role for the school keeper is a problem with the system. Report no other role can not be expected. Should therefore be preceded by a systematic improvement and training of experts in order to prevent school violence, and home and school, in terms of social support and measures.

• Explosives and Blasting
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• v.30 no.2
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• pp.52-58
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• 2012

A TBM launching shaft in DTL2 Contract 915 site is located in a typical hard Bukit Timah granite formation and lots of blasting work is required for shaft sinking. The original blast design used the electric detonator and ANFO blasts consisting of 30 holes per one blast with 1.5 m depth of drilling hole. However, significant delay of work and poor progress were expected due to the limitation of the number of blasting hole and strict vibration regulation on retaining systems. To overcome such constraints, an efficient new blasting method which can improve productivity and satisfy vibration limit was required. The revised blast design, using triple-deck blasts with electronic detonators and cartridge emulsion explosives, gives better construction performance and can reduce construction time. Such a new blasting technique can be effectively used for similar underground projects in the future where the volume of rock blasting is significant.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.865-870
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• 2003

A motion controller has been used variously in industry such as semiconductor manufacture equipment, industrial robot, assembly/conveyor line applications and CNC equipment. There are several types of controller in motion control. One of these is a PC-based motion controller such as PCI or ISA, and another is stand-alone motion controller. The PC bus-based motion controller is popular because of improving bus architectures and GUI (Graphic User Interface) that offer convenience of use to user. There are some problems in this. The PC bus-based solution allows for only one of the form factors, so it has a poor flexibility. The overall system package size is bigger than other motion control system. And also, additional axes of control require additional slot, however the number of slots is limited. Furthermore, unwieldy and many wirings come to connect plants or I/O. The stand-alone motion controller has also this limit of axes of control and wiring problems. To resolve these problems, controller must have capability of operating as stand-alone devices that resides outside the computer and it needs network capability to communicate to each motion device. In this paper, a network-based stand-alone motion system is proposed. This system integrates PC and motion controller into one stand-alone motion system, and uses CAN (Controller Area Network) as network protocol. Single board computer that is type of 3.5" FDD form factor is used to reduce the system size and cost. It works with Windows XP Embedded as operating system. This motion system operates by itself or serves as master motion controller that communicates to slave motion controller. The Slave motion controllers can easily connect to master motion system through CAN-network.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.36 no.3
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• pp.127-134
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• 2018

Electric transmission towers are facilities to transport electrical power from a plant to an electrical substation. The towers are connected using power lines that are installed with a proper sag by loosening the cable to lower the tension and to secure the sufficient clearance from the ground or nearby objects. The power line sag may extend over the tolerance due to the weather such as strong winds, temperature changes, and a heavy snowfall. Therefore the periodical mapping of the power lines is required but the poor accessibility to the power lines limit the work because most power lines are placed at the mountain area. In addition, the manual mapping of the power lines is also time-consuming either using the terrestrial surveying or the aerial surveying. Therefore we utilized multiple overlapping images acquired from a low-cost drone to automatically reconstruct the power lines in the object space. Two overlapping images are selected for epipolar image resampling, followed by the line extraction for the resampled images and the redundant images. The extracted lines from the epipolar images are matched together and reconstructed for the power lines primitive that are noisy because of the multiple line matches. They are filtered using the extracted line information from the redundant images for final power lines points. The experiment result showed that the proposed method successfully generated parabolic curves of power lines by interpolating the power lines points though the line extraction and reconstruction were not complete in some part due to the lack of the image contrast.

• The Journal of Engineering Geology
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• v.19 no.3
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• pp.401-408
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• 2009

Old national Road No. 59 that connects Danyang and Gagok has 35 dangerous cut slopes. It is relatively narrow and has a poor alignment. The torrential rains in 2002 and 2006 has caused numerous slope collapses, landslides and road settlements in this area. The old road's high risk level lead to the planning and construction of a new national road. During the construction of the new road in December 2006, the right side of Dugcheon Tunnel entrance has collapsed and tension cracks were observed on the district road above the tunnel. In order to determine the cause of failure, intensive field investigation and monitoring cracks were performed together with Lower Hemisphere Projection Analysis, Limit Equilibrium Analysis and Finite Difference Analysis.