• KSBB Journal
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• v.13 no.5
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• pp.577-584
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• 1998

A sensitive membrane strip assay for plasma lipoprotein cholesterol that can be performed without handling reagents has been investigated. We previously developed an assay system with immobilized enzymes (cholesterol esterase and cholesterol oxidase) on the surfaces of nitrocellulose membrane(1). In such a case, the amount of enzymes present on the membrane was limited by its surface area and, thus, the detection capability was relatively poor (> 50 mg/dL cholesterol). To overcome this problem, we devised a new system with non-immobilized enzymes by placing them within interstitial spaces of a celullose membrane pad in a dry state. Upon contact with sample medium, the enzymes were immediately dissolved and participated in the reactions with cholesterol in a liquid phase. We constructed a user-friendly system consisting of four membrane pads fro sample application, cholesterol decomposition, color development as signal, and medium absorption to invoke a continuous flow (sequential location from the bottom). A sample containing lipoproteins was added into the application pad by capillary action and transferred to the next pad for decomposition. The decomposition pad (namely, enzyme pad) contained a detergent (sodium cholate) for the destruction of lipoprotein particles, the two enzymes for cholesterol decomposition, and a chromogen (3,3'-diaminobenzidine). As a consequence of the enzyme reactions, hydrogen peroxide was produced, and then reacted in the presence of the chromogen with horseradish peroxidase immobilized on the signal generation pad. Finally, a colorimetric signal directly proportional to the cholesterol concentration was produced. The detection limit determined from this system under optimal conditions was at least 2 times lower than of the enzyme-immobilized system.

• International Journal of Highway Engineering
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• v.18 no.3
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• pp.33-45
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• 2016

PURPOSES : Concrete pavement has been used in the construction of the Jungbu expressway in 1987. More than 60% of the pavement on the expressway is currently made of concrete, but most has been used far beyond their design life. Pavement life has been extended through routine maintenance or overlay. However, the structural capacity of the pavement has reached its limit, and extensive rehabilitation/reconstruction with long time traffic blocking should be considered. The three following issues on concrete rehabilitation/reconstruction will be discussed: (1) economic comparison of asphalt inlay and asphalt overlay, (2) economic comparison preventive overlay on a section which is currently good and routine overlay on the section which will be poor, and (3) economic analysis of early-strength concrete when it is used in concrete reconstruction. METHODS : First, various life cycle cost analysis tools were compared, and the proper tool for the extensive rehabilitation/reconstruction was selected. Second, a sensitivity analysis of the selected tool was performed to find the influential input variables, which should be carefully selected in the analysis. Third, three case studies, which can be issues in the rehabilitation/reconstruction of the expressway concrete pavement in Korea, were performed. RESULTS : Asphalt overlay without milling the deteriorated concrete showed 18~25% lower life cycle cost than the current asphalt inlay with milling. The good current preventive overlay on the section was economically justified within the scope of this study. The construction cost limit of the early strength concrete was suggested to be economical for 1, 3, and 7 days of construction alternative opening. CONCLUSIONS : CA4PRS was a viable tool for comparing various rehabilitation/reconstruction issue alternatives. Several concrete issues associated with the rehabilitation/reconstruction of the deteriorated concrete pavement were discussed as mentioned above.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.8
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• pp.1384-1390
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• 2008

The TCP protocol is originally designed for wired network, however it performs very poor in wireless network due to different nature of wireless network from wired networks. In terms of TCP performance improvement in wireless Ad-hoc network, many researches show that small congestion window size of TCP connection can improve TCP performance. We propose a new TCP algorithm to improve TCP performance in wireless Ad-hoc network. The basic idea of our approach is that TCP receiver estimates the optimum window size and then sets congestion window limit of TCP sender to an optimum value by using the advertised window field in TCP ACK packet. From extensive computer simulation, the proposed algorithm shows superior performance than traditional TCP protocols in terms of packet delivery ratio and packet loss.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.18 no.3
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• pp.46-57
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• 2019

Generally speeding appeared to be the most contributing factor of fatalities occurred in work zones, and highway agencies in South Korea have concerned of the safety of workers and drivers in the poor circumstances. In this study, a portable variable message signs (PVMS) system as an alternative of control speeding in work zones was implemented. This study evaluated the safety effectiveness of the PVMS based on speeds and the compliance with the speed limit. Linear regression and logistic regression models were adopted to quantify the safety effect of the PVMS between the 'before' and 'after'. The results showed that most of points had statistically significant speeds reduction experience after PVMS installation. Also, the percentage of vehicle exceeding the speed limit by 10 km/h or more was decreased significantly between 50 and 80% in the 'after' periods compared to the 'before' periods. Therefore, the PVMS would be contributed to benefit safety in work zones which there is a difference in design speed of the adjacent normal section.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.6
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• pp.35-46
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• 2015

Improvement in domestic poultry production has a positive effect on the export competitiveness of the poultry industry. However, overproduction and enlargement of facilities to assure a supply increase a stocking density which make a poor environment in the broiler house. In particular, an intensive rearing environment is vulnerable to dust control that causes respiratory diseases, such as asthma, bronchitis, etc., to farmers and broilers. However, monitoring data and research for environment control are not adequate, and there are no air quality regulations in broiler houses in Korea. In this study, TSP, PM10, inhalable dust and respirable dust concentration were monitored according to season, age of broiler and broiler's activities. Air quality assessment was also performed in accordance with the threshold limit value by Donham et al. (2000). The TSP concentrations were 77.5 %, 219.7 % higher and PM10 concentrations were 121.2 %, 303.8 % higher when change of season and winter respectively than summer. There were significantly different concentrations according to season and age of broiler. Inhalable and respirable dust concentration were also clearly different according to the season and age of broiler. A high dust concentration was observed, specifically exceeding the threshold limit by 119 % in the winter. In the case of the broiler's motion was activity according to worker's access into the broiler house, concentration level was 769.6 % higher than broiler's motion was stable and exceeded the threshold limit. These results suggest that the worker should put on protective equipment to protect there's respiratory health in the broiler house.

• Nuclear Engineering and Technology
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• v.39 no.2
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• pp.133-142
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• 2007

The Electric Power Research Institute (EPRI) demonstrated a method for monitoring the performance of instrument channels in Topical Report (TR) 104965, 'On-Line Monitoring of Instrument Channel Performance.' This paper presents the results of several models originally developed by EPRI to monitor three nuclear plant sensor sets: Pressurizer Level, Reactor Protection System (RPS) Loop A, and Reactor Coolant System (RCS) Loop A Steam Generator (SG) Level. The sensor sets investigated include one redundant sensor model and two non-redundant sensor models. Each model employs an Auto-Associative Kernel Regression (AAKR) model architecture to predict correct sensor behavior. Performance of each of the developed models is evaluated using four metrics: accuracy, auto-sensitivity, cross-sensitivity, and newly developed Error Uncertainty Limit Monitoring (EULM) detectability. The uncertainty estimate for each model is also calculated through two methods: analytic formulas and Monte Carlo estimation. The uncertainty estimates are verified by calculating confidence interval coverages to assure that 95% of the measured data fall within the confidence intervals. The model performance evaluation identified the Pressurizer Level model as acceptable for on-line monitoring (OLM) implementation. The other two models, RPS Loop A and RCS Loop A SG Level, highlight two common problems that occur in model development and evaluation, namely faulty data and poor signal selection

• Proceedings of the Korean Geotechical Society Conference
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• 1999.11c
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• pp.85-94
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• 1999

Reinforced Soil Wall has several merits comparing with conventional retaining wall. The conventional method has the limit of wall height, ununiform settlement of the foundation ground, quality assurance of the embankment body, shortening of construction period, economical construction and so on. Basis of previous mentioned things reinforced soil wall is the substitutional method of conventional retaining wall and its necessity is continuously increasing. The embanking material used in reinforced soil wall is generally limited such as a good quality sandy soil, and in many case constructors have to transfer such a good embanking material from far away to construction site. As a result, they would pressed by time and economy. If poor soils could be used embanking material, for example, clayey soil produced in-situ by cutting and excavation, the economical merit of reinforced soil wall would be increased more and more. Likewise, a lot of study about laboratory experimental behavior of reinforced soil wall using a good quality soil is being performed, but is rare study about clayey soil containing much volume of fine particle relatively in korea. In this study, the authors investigated behavior of the geosynthetic reinforced and unreinforced soil walls using clayey soil as embanking material in view of horizontal movement of walls, bearing capacity and reinforcement stress.

• Journal of Embryo Transfer
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• v.27 no.2
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• pp.71-80
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• 2012

Biotechnologies for cloning animals and in vitro embryo production have the potential to produce biomedical models for various researches. Especially, pigs are a suitable model for xenotransplantation, transgenic production and various areas of reproductive research due to its physiological similarities to human. However, utilization of in vitro-produced embryos for transfer remains limited. Despite improvement over past few decades, obstacles associated with the production of good quality embryos in vitro still exist which limit the efficiency of cloning. One of major problems includes improper in vitro maturation (IVM) and culture (IVC). Oxidative stress caused from in vitro culture conditions contributes to inadequate IVM and IVC which leads to poor developmental competence of oocytes, failure of fertilization and embryo development. To reduce the oxidative stress, various antioxidants have been used to IVM and IVC system. However, limited information is available on the effects of resveratrol on livestock reproductions. Resveratrol is a polyphenolic natural product and well known as an antioxidant in foods and beverages (e.g. in grapes and red wine). Resveratrol is known to be cardioprotective, anticarcinogenic, anti-inflammatory, antioxidant and antiapoptotic. This paper will review the effects of resveratrol on in vitro maturation of oocytes and embryo development.

• Journal of Institute of Control, Robotics and Systems
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• v.20 no.2
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• pp.143-148
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• 2014

Conventional wireless power transfer methods based on coupled magnetic fields need a complex winding structure on the surface of the energy transfer and shows poor efficiency near metal objects due to the eddy current effect. In this study, to mitigate these problems, we investigate an electric field-coupled power transmission system, which is less prone to metal object problems and EMI. Because of the fundamental physical limit in the size of link capacitances, a half-bridge converter with an impedance matching transformer is proposed and the design procedure is derived to provide a soft-switching scheme. Hardware implementation shows that the proposed scheme with a pair of 10cm by 10cm copper plate can power a 1.4W USB FAN in a separation of 0.2mm by using insulating paper when driven by 227 kHz gate pulse.

• Journal of the East Asian Society of Dietary Life
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• v.25 no.3
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• pp.484-491
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• 2015

This study investigated the quality variation characteristics of Ssamjang containing Cheongkukjang, in order to improve the degree of preference of Cheongkukjang. The amount of Cheongkukjang was set at 1% based on sensory evaluation. Ssamjang containing Cheongkukjang was stored at $37^{\circ}C$ for 13 weeks, after which quality variation characteristics were weekly. During storage for 13 weeks, physicochemical quality characteristics, moisture content, and pH of Ssamjang containing Cheongkukjang slightly decreased, whereas salt content did not change. Amino nitrogen content slightly increased by 1 week but decreased by 3 weeks and then increased by 5 weeks. For microbiology quality characteristics, viable cell counts and total cell counts of B. cereus were unchanged. For sensory quality characteristics, shape quality was poor after 13 weeks while mold, drying phenomenon, and swelling phenomenon were not observed. Therefore, physicochemical quality and microbiology quality of Ssamjang containing Cheongkukjang were unchanged during storage for 13 weeks, and the storage limit was determined to be 12 weeks according to sensory quality evaluation.