• 제목/요약/키워드: Polystyrene Bead

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Qualitative Enzyme-Linked Immunosorbent Assay (ELISA) for the Diagnosis of Edwardsiellosis (Edwardsiellosis의 진단을 위한 정성적 ELISA법)

  • Kim, Myoung-Sug;Hwang, Eun-A;Huh, Min-Do;Jeong, Hyun-Do
    • Journal of fish pathology
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    • 제12권1호
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    • pp.24-31
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    • 1999
  • Optimization and standardization of solid phase enzyme immunoassay were done for the diagnosis of edwardsiellosis in fish. The analyzed degree of immobilized antibody on surface of solid phase with peroxidase saturation method showed the optimized result by using partially purified $50{\mu}g/ml$ of rabbit anti-E. tarda Edk-2 antibody in sodium bicarbonate buffer for overnight incubation to cover the surface of polystyrene beads. Optimized immunoreaction was observed in the treatment of $50{\mu}g/ml$ of biotin conjugated antibody followed extravidin-peroxidase diluted 1 : 2,000 in PBS. The detectable concentrations of the this method were $1{\times}10^5$ cells/ml and $1{\times}10^5$ cells/ml expressed as the source of antigen amount for EDTA extraction and heat extraction, respectively. High cross-reaction of solid phase ELISA with the prepared rabbit and-E. tarda Edk-2 was observed against E. tarda strains isolated from flounder suffering from edwardsiellosis in aquatic farms of Korea. It suggested that the potential of this solid phase of ELISA technique is very powerful for the application to different strains of E. tarda isolated in farms of many different areas.

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A Study on the Development Lightweight Aggregate using Clink Ash for Reduction in Freezing Ground (지반의 동결저감 대책기술을 수립하기 위한 클링커애쉬 인공경량골재 개발에 관한 연구)

  • Moon, Jong-Wook
    • Fire Science and Engineering
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    • 제23권6호
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    • pp.116-125
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    • 2009
  • This study is progressed function ratio, it's trued taste by an experiment to present data for human work light weight aggregate development that use clink ash progressed liquid limit, small success limit, wear loss in quantity, sand equivalent, sieve cutting examination. 80 : 20's match of function rain examination is 1.4, and that use rubble Goljae as ckink ash lightweight aggregate's capacity ratio increases by 1.0 increase of function rain many. Also, examination multiplied delicate flavor gradually according to increase of the mixing rate, and absorption coefficient increased. This is judged by phenomenon that appear by special quality upper clink ash of polystyrene bid and porosity's increase between lightweight aggregate. It is case that use aggregate of wear loss in quantity is 13.5 in sand equivalent and a wear loss in quantity experiment and although case that mix 20% increases by 14.4, this phenomenon by weak tissue of lightweight aggergate be judge. When it's as a these experiment, the statue prevention floor of a street improvement specifications is prescribing so that satisfy by sand equivalent 20, CBR 10. This is showed result that this satisfies in quality standard all in match experiment ago that see.

An Experimental Study on a Performance Evaluation of Internal Insulation of Buildings Over 20 Years Old (20년 이상 경과된 노후건축물의 단열재 성능평가에 관한 실험적 연구)

  • Kim, Hyun-Jin;Choi, Se-Jin
    • Journal of the Korea Institute of Building Construction
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    • 제19권6호
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    • pp.539-547
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    • 2019
  • Recently, the international community signed a climate change agreement to prevent global warming. Yet currently, the fossil fuels have been widely used in to supply building energy for cooling and heating. The Green Building certification (G-SEED), an energy efficiency rating for new or existing buildings requires that buildings meet certain conditions. Insulation is used as a building material to reduce the energy supply to buildings and to improve the thermal insulation, and it accounts for more than 90% of the total heat resistance provided by the building surface components that meet the energy-saving design standards of new buildings. In this investigation, a performance evaluation study was conducted through an experimental study by directly extracting the foam polystyrene insulation on-site during the remodeling of a building that was in the range of 22~38 years old. Through tests, it was found that the thermal conductivity of the extrusion method insulation (XPS) was reduced by 48% and the compressive strength of XPS decreased by 36% compared to KS M 3808, which is the initial quality standard. For bead method insulation (EPS) with a thickness of 50mm, the thermal conductivity, the compressive strength, and flexural failure load were similar to the initial quality standard. Therefore, in the calculation of the primary energy requirement per unit area per year, the performance of bead method insulation can be estimated simply by considering the thickness of the insulation, while a correction factor that considers its performance deterioration should be applied when extrusion method insulation is used.

Effect of BLU Ingredient on Electrical and Optical Properties of Light Diffusing Film used for TFT-LCD TV (백라이트 유니트 구성요소가 TFT-LCD TV용 광확산필름의 전기, 광학적 특성에 미치는 영향)

  • Ahn, Cheol-Heung;Kim, Geon-Joong
    • Applied Chemistry for Engineering
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    • 제17권4호
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    • pp.397-402
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    • 2006
  • We have investigated the effect of each ingredient in the light diffusing film (LDF) which was used as backlight unit (BLU) of TFT-LCD TV on physical, electrical, thermal and optical properties of LDF. In anti-blocking layer, the excellent anti-blocking ability was obtained when 0.5~1.5 wt% of acrylic bead was added, and good decay-time and water-stability were shown when 0.8 wt% of tertiary ammonium salt was added. Optimal results for adhesion strength, curing rates and flexibility on the surface of PET film have been obtained in the light diffusing layer by using acrylic polyol as a binder resin, and by addition of 30~35 wt% non-yellowing type HDI crosslinker. In addition, the highest normal luminance value was obtained by addition of 250 wt% poly-dispersive polystyrene ($20{\mu}m$ PS) and polymethylmethacrylate ($20{\mu}m$ PMMA) beads into the binder resin (100 wt%). The higher normal luminance could be got for PMMA beads than PS beads because of the transmittance difference.

Highly Sensitive Gas Sensors Based on Nanostructured $TiO_2$ Thin Films

  • Jang, Ho-Won;Mun, Hui-Gyu;Kim, Do-Hong;Sim, Yeong-Seok;Yun, Seok-Jin
    • Proceedings of the Materials Research Society of Korea Conference
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    • 한국재료학회 2011년도 춘계학술발표대회
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    • pp.16.1-16.1
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    • 2011
  • $TiO_2$ is a promising material for gas sensors. To achieve high sensitivities, the material should exhibit a large surface-to-volume ratio and possess the high accessibility of the gas molecules to the surface. Accordingly, a wide variety of porous $TiO_2$ nanomaterials synthesized by wet-chemical methods have been reported for gas sensor applications. Nonetheless, achieving the large-area uniformity and comparability with well-established semiconductor production processes of the methods is still challenging. An alternative method is soft-templating which utilizes nanostructured inorganic or organic materials as sacrificial templates for the preparation of porous materials. Fabrication of macroporous $TiO_2$ films and hollow $TiO_2$ tubes by soft-templating and their gas sensing applications have been reported recently. In these porous materials composed of assemblies of individual micro/nanostructures, the form of links or necks between individual micro/nanostructures is a critical factor to determine gas sensing properties of the material. However, a systematic study to clarify the role of links between individual micro/nanostructures in gas sensing properties of a porous metal oxide matrix is thoroughly lacking. In this work, we have demonstrated a fabrication method to prepare highly-ordered, embossed $TiO_2$ films composed of anatase $TiO_2$ hollow hemispheres via soft-templating using polystyrene beads. The form of links between hollow hemispheres could be controlled by $O_2$ plasma etching on the bead templates. This approach reveals the strong correlation of gas sensitivity with the form of the links. Our experimental results highlight that not only the surface-to-volume ratio of an ensemble material composed of individual micro/nanostructures but also the links between individual micro/nanostructures play a critical role in evaluating the sensing properties of the material. In addition to this general finding, the facileness, large-scale productivity, and compatability with semiconductor production process of the proposed fabrication method promise applications of the embossed $TiO_2$ films to high-quality sensors.

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Multiwalled Carbon Nanotubes Functionalized with PS via Emulsion Polymerization

  • Park, In-Cheol;Park, Min;Kim, Jun-Kyung;Lee, Hyun-Jung;Lee, Moo-Sung
    • Macromolecular Research
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    • 제15권6호
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    • pp.498-505
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    • 2007
  • This study demonstrated the in-situ functionalization with polymers of multi-walled carbon nanotubes (MWNTs) via emulsion polymerization. Polystyrene-functionalized MWNTs were prepared in an aqueous solution containing styrene monomer, non-ionic surfactant and a cationic coupling agent ([2-(methacryloyloxy)ethyl]trime-thylammonium chloride (MATMAC)). This process produced an interesting morphology in which the MWNTs, consisting of bead-string shapes or MWNTs embedded in the beads, when polymer beads were sufficiently large, produced nanohybrid material. This morphology was attributed to the interaction between the cationic coupling agent and the nanotube surface which induced polymerization within the hemimicellar or hemicylindrical structures of surfactant micelles on the surface of the nanotubes. In a solution containing MATMAC alone without surfactant, carbon nanotubes (CNTs) were not well-dispersed, and in a solution containing only surfactant without MATMAC, polymeric beads were synthesized in isolation from CNTs and continued to exist separately. The incorporation of MATMAC and surfactant together enabled large amounts of CNTs (> 0.05 wt%) to be well-dispersed in water and very effectively encapsulated by polymer chains. This method could be applied to other well-dispersed CNT solutions containing amphiphilic molecules, regardless of the type (i.e., anionic, cationic or nonionic). In this way, the solubility and dispersion of nanotubes could be increased in a solvent or polymer matrix. By enhancing the interfacial adhesion, this method might also contribute to the improved dispersion of nanotubes in a polymer matrix and thus the creation of superior polymer nanocomposites.

Effect of Operating Parameters on the Physical Properties of Activated Carbon Manufactured with Bead-Type Polymer Resin (구형 고분자수지로 활성탄제조 시 운전인자의 영향)

  • Lee, Gangchoon;Yoon, Taekyung;Shon, Zangho
    • Clean Technology
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    • 제19권3호
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    • pp.279-286
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    • 2013
  • Using a cation exchange resin of polystyrene-DVB copolymer as a carbon source for the production of activated carbon, the effects of operating parameters on the physical properties of activated carbon such as specific surface area and specific pore volume were experimentally studied. In carbonization process specific surface area and specific pore volume of carbonized product decreased with the increase of carbonization temperature and time. They were proportional to activation temperature and time up to the specific temperature and time in activation process. In carbonization process the increase of heating rate gave negative effect to the properties. The properties of activated product, on the contrary, increased with the heating rate in carbonization process. The properties of activated carbon manufactured with the resin exchanged with divalent cations were lower than those with raw resin.

Changes in Insulation Performance of Organic Insulating Materials for Building Construction by Accelerated Durability Test Conditions (가속내구성 조건에 따른 건축용 유기계 단열재의 단열성능 변화)

  • Lim, Soon-Hyun;Lee, Gun-Cheol
    • Journal of the Korea Institute of Building Construction
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    • 제16권6호
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    • pp.595-601
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    • 2016
  • The insulation performance of the insulation currently used in building structures is reflected only during design based on initial performance and the reduction in heat insulation performance due to the degradation of long-term durability is not reflected. This study reviewed the degradation of heat insulation performance due to the durability degradation of insulating materials through the accelerated durability test. The study findings showed that the foamed polystyrene insulation bead method did not show performance degradation due to aging in the standard environmental condition and laboratory accelerated test condition but the performance is degraded in the freeze-thaw test condition. On the other hand, in the case of the extrusion method, the degradation of the heat insulation performance was less in the freeze-thaw test condition, but the rapid performance degradation was caused by the release of the internal gas at the beginning of aging. In addition, the hard polyurethane foam insulation showed better initial insulation performance than other insulation materials, but the performance was found to be degraded somewhat under laboratory accelerated test conditions and freeze-thaw test conditions.

A Study on Engineering Characteristics of Geotechnical Material Using By-Product Lime and Pieces of Waste EPS Beads (석회부산물 및 폐 EPS beads를 활용한 지반재료의 공학적 특성에 관한 연구)

  • Bang, Yoon-Kyung;Park, Min-Yong;Yoon, Chang-Jin;Kim, Hong-Taek
    • Journal of the Korean Geotechnical Society
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    • 제20권2호
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    • pp.97-105
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    • 2004
  • The purpose of this study is to provide the ways of recycling of by-product limes as lightweight fill, backfill materials, and lightweight blocks by performing experimental study. New lightweight fill materials and blocks were devised by mixing by-product lime, weathered granite soil, small pieces of waste EPS, and Portland cement. Physical, geotechnical, and environmental properties of the lightweight mixed soils and blocks were analysed by laboratory experiments for mixed samples manufactured with various mixing ratios. KMS tests were also performed to evaluate the concentration variation of the chemical components of the light weight blocks leachates. It is expected that this study will contribute to resolving the problem of by-product lime disposal as well as to recycling the by-product limes as fill materials and blocks.

Enhancing the Performance of Polypropylene Fiber Reinforced Cementitious Composite Produced with High Volume Fly Ash (폴리프로필렌 섬유로 보강된 하이볼륨 플라이애시 시멘트 복합재료의 성능 향상 기법)

  • Lee, Bang Yeon;Bang, Jin Wook;Kim, Yun Yong
    • Journal of the Korea institute for structural maintenance and inspection
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    • 제17권3호
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    • pp.118-125
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    • 2013
  • The synthetic fibers including Polyvinyl alcohol and Polyethylene fibers have been successfully used in the manufacture of high ductile fiber reinforced cementitious composites. Polypropylene (PP) fiber has also been used in composites, not for the purpose of achieving a high level of tensile ductility but to improve the fire resistance performance of concrete exposed to high temperatures. This paper discusses the method for enhancing the performance of composites supplemented with PP fiber. Five types of mixture proportions were designed with high volume fly ash for testing the performance of composites. Type I cement and fly ash F were used as binding materials. The water-to-binder ratio was 0.23~0.25, and the amount of PP fiber used was 2 vol%. Polystyrene bead were also used to increase the tensile ductility of composites. A series of experiments including slump, density, compression and uniaxial tension tests were performed to evaluate the performance of cementitious composites supplemented with PP fiber. From the test results, it was exhibited that the performance of composites supplemented with PP fiber can be enhanced by adopting the mechanics and statistics theory.