• 한국전자통신학회논문지
• /
• 제4권2호
• /
• pp.101-107
• /
• 2009

현대사회는 산업화로 인해 다양한 재해를 접하게 되고, 그 피해를 최소화하기 위해 많은 연구가 이루어지고 있다. 재난의 예방을 위해서는 위험 상황에 대한 정확한 판단과, 그 상황 발생에 즉각적인 대처가 가능한 시스템의 개발이 필요하다. 현재 유비쿼터스 환경하의 상황정보시스템에 대한 다양한 연구들이 진행되고 있다. 그러나 다중 상황에 대한 모니터링 및 처리가 가능한 시스템에 대한 연구가 미흡하며, 이로 인해 다중상황에 대한 신속한 대처가 이루어지지 않고 있다. 본 논문에서는 다중상황의 처리를 위해 각종 센서로부터 데이터를 취득하여 시간 마킹 처리 후 데이터베이스에 저장하고, 사용자의 요구에 따라 추상화된 Context를 사용자에게 제공할 수 있는 다중상황 처리를 위한 RFID/USN 미들웨어를 시스템을 설계한다.

• Journal of Information Display
• /
• 제2권2호
• /
• pp.27-31
• /
• 2001

Transistor slicing refers to the use of multiple smaller transistors in implementing a large MOS transistor. What is special about transistor slicing is that it can reduce the effects of device non-uniformity introduced during the fabrication process. The paper presents the idea of transistor slicing and analyzes the benefits of using transistor slicing in the context of Poly-Si TFT-LCD driving.

• 대한산업공학회지
• /
• 제29권2호
• /
• pp.179-189
• /
• 2003

Gate poly-silicon critical dimension is a prime characteristic of a metal-oxide-semiconductor field effect transistor. It is important to achieve the uniformity of gate poly-silicon critical dimension in order that a semiconductor device has acceptable electrical test characteristics as well as a semiconductor wafer fabrication process has a competitive net-die-per-wafer yield. However, on gate poly-silicon critical dimension, the complexity associated with a semiconductor wafer fabrication process entails hierarchical variance components according to run-to-run, wafer-to-wafer and even die-to-die production unit changes. Specifically, estimates of the hierarchical variance components are required not only for disclosing dominant sources of the variation but also for testing the wafer-level uniformity. In this paper, two experimental designs, a two-stage nested design and a randomized complete block design are considered in order to estimate the hierarchical variance components. Since gate poly-silicon critical dimensions are collected from fixed die positions within wafers, a factor representing die positions can be regarded as fixed in linear statistical models for the designs. In this context, the two-stage nested design also checks the wafer-level uniformity taking all sampled runs into account. In more detail, using variance estimates derived from randomized complete block designs, Duncan's multiple range test examines the wafer-level uniformity for each run. Consequently, a framework presented in this study could provide guidelines to practitioners on estimating the hierarchical variance components and testing the wafer-level uniformity in parallel for any characteristics concerned in semiconductor wafer fabrication processes. Statistical analysis is illustrated for an experimental dataset from a real pilot semiconductor wafer fabrication process.

• Molecules and Cells
• /
• 제45권4호
• /
• pp.257-272
• /
• 2022

In addition to inducing apoptosis, caspase inhibition contributes to necroptosis and/or autophagy depending on the cell type and cellular context. In macrophages, necroptosis can be induced by co-treatment with Toll-like receptor (TLR) ligands (lipopolysaccharide [LPS] for TLR4 and polyinosinic-polycytidylic acid [poly I:C] for TLR3) and a cell-permeable pan-caspase inhibitor zVAD. Here, we elucidated the signaling pathways and molecular mechanisms of cell death. We showed that LPS/zVAD- and poly I:C/zVAD-induced cell death in bone marrow-derived macrophages (BMDMs) was inhibited by receptor-interacting protein kinase 1 (RIP1) inhibitor necrostatin-1 and autophagy inhibitor 3-methyladenine. Electron microscopic images displayed autophagosome/autolysosomes, and immunoblotting data revealed increased LC3II expression. Although zVAD did not affect LPS- or poly I:C-induced activation of IKK, JNK, and p38, it enhanced IRF3 and STAT1 activation as well as type I interferon (IFN) expression. In addition, zVAD inhibited ERK and Akt phosphorylation induced by LPS and poly I:C. Of note, zVAD-induced enhancement of the IRF3/IFN/STAT1 axis was abolished by necrostatin-1, while zVAD-induced inhibition of ERK and Akt was not. Our data further support the involvement of autocrine IFNs action in reactive oxygen species (ROS)-dependent necroptosis, LPS/zVAD-elicited ROS production was inhibited by necrostatin-1, neutralizing antibody of IFN receptor (IFNR) and JAK inhibitor AZD1480. Accordingly, both cell death and ROS production induced by TLR ligands plus zVAD were abrogated in STAT1 knockout macrophages. We conclude that enhanced TRIF-RIP1-dependent autocrine action of IFNβ, rather than inhibition of ERK or Akt, is involved in TLRs/zVAD-induced autophagic and necroptotic cell death via the JAK/STAT1/ROS pathway.

• BMB Reports
• /
• 제35권3호
• /
• pp.262-266
• /
• 2002

A non-isotopic neutravidin-based reverse transcriptase (RT) assay adapted for high throughput screening of HIV activity is described. Using a 96-well microtitre plate, HIV particles are lysed and the RT enzyme released into a reaction mixture containing poly(A) RNA, biotinylated oligo d(T) and fluorescein-labelled dUTP (FI-dUTP). With poly(A) as a template and oligo d(T) as primer, the viron RT incorporates FI-dUTP into an elongating DNA strand. The resulting product is captured on a neutravidin-coated 96-well plate and the unincorporated nucleotides removed by a series of washing steps. A simple ELISA is subsequently performed using a monoclonal antifluorescein antibody conjugated to alkaline phosphatase. Quantification of RT activity is facilitated by a colorimetric readout. The assay was validated in the context of a diagnostic HIV-1 phenotyping assay. Using supernatants from HIV-1 infected lymphocyte cultures the assay was shown to be as sensitive as a radioactive assay and the RT activity correlated well with levels of cell-asociated HIV-p24. Importantly, even minor reductions of RT activity by virus variants with reduced fitness could be distinguished.

• Bulletin of the Korean Chemical Society
• /
• 제28권3호
• /
• pp.397-402
• /
• 2007

Restenosis after percutaneous coronary intervention (PCI) continues to be a serious problem in clinical cardiology. To solve this problem, drug eluting stents (DES) with antiproliferative agents have been developed. Variable local drug delivery systems in the context of stenting require the development of stent manufacture, drug pharmacology and coating technology. We have worked on a system that integrates electrophoretic deposition (EPD) technology with the polymeric nanoparticles in DES for local drug delivery and a controlled release system. The surface morphology and drug loading amount of DES by EPD have been investigated under different operational conditions, such as operation time, voltage and the composition of media. We prepared poly-D,L-lactide-co-glycolic acid (PLGA) nanoparticles embedded with curcumin, which was done by a modified spontaneous emulsification method and used polyacrylic acid (PAA) as a surfactant because its carboxylic group contribute negative charge to the surface of CPNPs (?53.5 ± 5.8 mV). In the process of ‘trial and error' endeavors, we found that it is easy to control the drug loading amount deposited onto the stent while keeping uniform surface morphology. Accordingly, stent coating by EPD has a wide application to the modification of DES using various kinds of nanoparticles and drugs.

• 한국유체기계학회 논문집
• /
• 제17권2호
• /
• pp.30-34
• /
• 2014

Membrane distillation (MD) is a thermal driven separation process in which separation a hydrophobic membrane is a barrier for the liquid phase, letting the vapor phase pass through the membrane pores. Therefore, a porous and hydrophobic membrane should be used in membrane distillation. MD cannot work if water penetrates into the pores of the membrane (membrane wetting). Accordingly, it is necessary to prevent wetting of MD membranes and to remove water inside the pores of the wetted membranes if possible. In this context, our study aimed to develop methods to recover wetted membranes in MD processes. Poly-vinylidene fluoride (PVDF) membranes were used in this study. A laboratory-scale direct contact MD (DCMD) system was used to examine the effect of operating parameters on wetting. For dewetting the wetted membranes, specific techniques including the use of high temperature air were applied. The performances of the membranes before and after dewetting were compared in terms of flux, salt rejection and liquid entry pressure(LEP). The surface morphology of dewetted membrane was confirmed by scanning electron microscope (SEM).

• Nutrition Research and Practice
• /
• 제14권3호
• /
• pp.203-217
• /
• 2020

BACKGROUND/OBJECTIVE: Centella asiatica, also known as Gotu kola, is a tropical medicinal plant native to Madagascar, Southeast Asia, and South Africa. It is well known to have biological activities, including wound healing, anti-inflammatory, antidiabetic, cytotoxic, and antioxidant effects. The purpose of this study was to determine the efficacy of extracts of C. asiatica against age-related eye degeneration and to examine their physiological activities. MATERIALS/METHODS: To determine the effects of CA-HE50 (C. asiatica 50% EtOH extract) on retinal pigment cells, we assessed the cytotoxicity of CoCl₂ and oxidized-A2E in ARPE-19 cells and observed the protective effects of CA-HE50 against N-methyl-N-nitrosourea (MNU)-induced retinal damage in C57BL/6 mice. In particular, we measured factors related to apoptosis and anti-oxidation and the protein levels of rhodopsin/opsin. We also measured glucose uptake to characterize glucose metabolism, a major factor in cell protection. RESULTS: Induction of cytotoxicity with CoCl₂ and oxidized-A2E inhibited decreases in the viability of ARPE-19 cells when CA-HE50 was administered, and promoted glucose uptake under normal conditions (P < 0.05). In addition, CA-HE50 inhibited degeneration/apoptosis of the retina in the context of MNU-induced toxicity (P < 0.05). In particular, CA-HE50 at 200 mg/kg inhibited the cleavage of pro-caspase-3 and pro-poly (ADP-ribose)-polymerase and maintained the expressions of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 similar to normal control levels. Rhodopsin/opsin expression was maintained at a higher level than in normal controls. CONCLUSION: A series of experiments confirmed that CA-HE50 was effective for inhibiting or preventing age-related eye damage/degeneration. Based on these results, we believe it is worthwhile to develop drugs or functional foods related to age-related eye degeneration using CA-HE50.

• Nuclear Engineering and Technology
• /
• 제51권8호
• /
• pp.1905-1915
• /
• 2019

This paper investigated the influence of simple support girds on flow, irrespective of having mixing vanes, in a 1 × 3 array rod bundle by using CFD methodology and the most accurate turbulence model which could reflect the actual physics of the flow was determined. In this context, a CFD model was created simulating the experimental studies on a single-phase flow [1] and the results were compared with the experimental data. In the first part of the study, influence of mesh was examined. Tetra, hybrid and poly type meshes were analyzed and convergence study was carried out on each in order to determine the most appropriate type and density. k - ε Standard and RSM LPS turbulence models were used in this section. In the second part of the study, the most appropriate turbulence model that could reflect the physics of the actual flow was investigated. RANS based turbulence models were examined using the mesh that was determined in the first part. Velocity and turbulence intensity results obtained on the upstream and downstream of the spacer grid at -3d_h, +3d_h and +40d_h locations were compared with the experimental data. In the last section of the study, the behavior of flow through the spacer grid was examined and its prominent aspects were highlighted on the most appropriate turbulence model determined in the second part. Results of the study revealed the importance of mesh type. Hybrid mesh having the largest number of structured elements performed remarkably better than the other two on results. While comparisons of numerical and experimental results showed an overall agreement within all turbulence models, RSM LPS presented better results than the others. Lastly, physical appearance of the flow through spacer grids revealed that springs has more influence on flow than dimples and induces transient flow behaviors. As a result, flow through a simple support grid was examined and the most appropriate turbulence model reflecting the actual physics of the flow was determined.

• 한국조경학회지
• /
• 제51권1호
• /
• pp.29-41
• /
• 2023

국내 조경학 연구에서 자연성 개념은 물리적 공간에서부터 인식적 체계까지 그 범주가 방대하여 용어를 정의하기에 어려움이 있다. 본 연구는 체계적 문헌 고찰(systematic review, SR)을 통하여 문헌에서 활용된 '자연성(naturalness)'의 개념과 평가 속성을 정리하고 자연성의 의미를 구성하는 개별 속성의 범위를 파악하였다. 또한, 선행연구에서 구분한 개별 속성을 인지언어학 연구 방식의 하나인 의미 연쇄(meaning chain) 원리로 파악하여 국내 조경학 논문 중 자연성을 대상으로 한 논문들에 적용하여 다의적 체계를 정리하였다. 의미 연쇄(meaning chain)는 원형의미를 중심으로 가족 유사성에 기인하여 연쇄적으로 의미가 확장되어 가는 단어를 파악하기에 적합한 방식으로서, 자연성 평가 항목 분류에 따라 그 차원을 구분하고 국내 학계에서 논의되는 자연성 개념의 다의적 의미 연쇄 체계를 고찰하였다. 연구의 결과는 다음과 같다. 첫째, 국외 조경학 문헌을 통해 추출한 자연성의 속성은 원생상태로 인지되는 자연, 인위적 개입이 없는 상태로서의 자연, 시각적 경관으로서의 자연, 경험으로서의 자연 4개 영역으로 분류되며 세부 13개 속성으로 정리되었다. 둘째, 이러한 세부속성은 국내 조경학 연구에도 전반적으로 부합하나 그 구체적 사례가 달랐고, 시간의 축적 인지에 있어 한국적 맥락이 제시되었으며, 자연성 속성 간 상호충돌이 있을 수 있음을 시사하였다.