• Animal cells and systems
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• v.9 no.2
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• pp.79-85
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• 2005

Oxidized abasic residues arise as a major class of DNA damage by a variety of agents involving free radical attack and oxidation of deoxyribose sugar components. 2-deoxyribonolactone (dL) is a C1'-oxidized abasic lesion implicated in DNA strand scission, mutagenesis, and covalent DNA-protein cross-link (DPC). We show here that mammalian cell-free extract give rise to stable DPC formation that is specifically mediated by dL residue. When a duplex DNA containing dL at the site-specific position was incubated with cell-free extracts of Po ${\beta}-proficient$ and -deficient mouse embryonic fibroblast cells, the formation of major dL-mediated DPC was dependent on the presence of DNA polymerase (Pol) ${\beta}$. Formation of dL-specific DPC was also observed with histones and FEN1 nuclease, although the reactivity in forming dL-mediated DPC was significantly higher with Pol ${\beta}$ than with histones or FEN1. DNA repair assay with a defined DPC revealed that the dL lesion once cross-linked with Pol ${\beta}$ was resistant to nucleotide excision repair activity of cell-free extract. Analysis of nucleotide excision repair utilizing a model DNA substrate containing a (6-4) photoproduct suggested that excision process for DPC was inhibited because of DNA single-strand incision at 5' of the lesion. Consequently DPC mediated by dL lesion may not be readily repaired by DNA excision repair pathway but instead function as unusual DNA damage causing a prolonged DNA strand break and trapping of the major base excision repair enzyme.

• Food Engineering Progress
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• v.13 no.3
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• pp.169-175
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• 2009

Water soluble polysaccharides (WSP) and arabinogalactan of Portulaca oleracea L. (POL) were increased after extrusion and commercial cellulase treatment. Arabinose and galactose content increased more about 1.5 times than those of raw POL, and rhamnose also increased about 2.6 times in WSP. High molecular weight fraction (I) of POL depending on extrusion condition including Ext I, Ext II and Ext III degraded into low molecular weight fraction (II) about 37, 29, and 26%, respectively, ranged from 67,000-69,000 Da of molecular weight. Especially, the molecular weight and composition of WSP with extruded, were increased from 9 to 13% in low molecular weight fraction, compared to those of raw POL. Solubilization and degradation of polysaccharides were a directly propotional to specific mechanical energy in POL extrusion. WSP obtained by extrusion at Ext I and Ext II were found to be effective antioxidants in different in vitro assays with regards to 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC). However, these results suggest that WSP obtained using extrusion and subsequent enzymatic treatment may be an effective method to produce arabinogalactan from POL and be used as a functional food ingredients.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8177-8186
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• 2016

The present study was aimed at determining the effects of alkylating and oxidative stress inducing agents on a newly identified variant of DNA polymerase beta ($pol{\beta}{\Delta}_{208-304}$) specific for ovarian cancer. $Pol{\beta}{\Delta}_{208-304}$ has a deletion of exons 11-13 which lie in the catalytic part of enzyme. We compared the effect of these chemicals on HeLa cells and HeLa cells stably transfected with this variant cloned into in pcDNAI/neo vector by MTT, colony forming and apoptosis assays. $Pol{\beta}{\Delta}_{208-304}$ cells exhibited greater sensitivity to an alkylating agent and less sensitivity towards $H_2O_2$ and UV when compared with HeLa cells alone. It has been shown that cell death in $Pol{\beta}{\Delta}_{208-304}$ transfected HeLa cells is mediated by the caspase 9 cascade. Exon 11 has nucleotidyl selection activity, while exons 12 and 13 have dNTP selection activity. Hence deletion of this part may affect polymerizing activity although single strand binding and double strand binding activity may remain same. The lack of this part may adversely affect catalytic activity of DNA polymerase beta so that the variant may act as a dominant negative mutant. This would represent clinical significance if translated into a clinical setting because resistance to radiation or chemotherapy during the relapse of the disease could be potentially overcome by this approach.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.219.1-219.1
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• 2003

RNA polymerase II (pol II) is known to cycle between hyperphosphorylated and hypophosphorylated forms during transcription cycle. These extensive phosphorylation/dephosphorylation event occurs in the C-terminal domain (CTD) of the largest subunit of pol II which consists of a tandemly repeated heptapeptide motif with consensus of YSPTSPS. (omitted)

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.8
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• pp.4183-4186
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• 2012

Background: Ovarian cancer is the number one killer among all the gynecological cancers. We undertook association study to identify potential alterations in the genomic DNA of a DNA repair gene, DNA polymerase beta ($pol{\beta}$), involved in base excision repair (BER), in ovarian carcinomas of patients from Haldia, India. Mutations, splice variants have been reported earlier in different tumors other than ovarian tumors. Aim: In this study we explored the possibility of association of any mutation of $pol{\beta}$ (Exon 8) with prognosis in 152 ovarian cancer samples. Results: Alteration in the exon 8 region (Exon 8:468, $A{\rightarrow}C$; 15.1%) was noted among fifty seven polymorphism positive samples. Alteration in the intervening sequence 8 (IVS8, -25, $A{\rightarrow}C$; 3.9%) was also noted. All alterations are heterozygous in nature. Conclusions: We found no significant association among the samples from serous type, stage IV, and the $pol{\beta}$ mutations ($P{\leq}0.01$). Only a slight tendency of association was evident between IVS8, -25, A to C; and stage III. Further analysis with a larger number of samples is needed.

• Korean Journal of Microbiology
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• v.43 no.1
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• pp.1-6
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• 2007

We describe a multiplex PCR method that can detect and differentiate simultaneously four different kinds of DNA viruses, Epstein-Barr virus (EBV), cytomegalovirus (CMV), hepatitis B virus (HBV) and parvovirus B19 (B19). Primers for the multiplex PCR reaction were designed to amplify specific regions of the EBV (pol), CMV (pol), HBV (pol) and B19 (ns) viral genomes and used to simultaneously detect individual viruses. In order to achieve optimal sensitivity and specificity for multiplex PCR, the thermo-cycling parameters, primer sequences, and concentration of each reaction components were optimized systematically. The sensitivity of the detection method ranged between 5 and 10 copies of viral genome with a mixture of multiple primer pairs. Furthermore, this highly sensitive test showed no cross-reactivity among the four viruses. Thus, the results obtained in this study provide evidence that the assay system is a good tool for supporting the diagnosis of viral infection and contamination.

• Journal of Power Electronics
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• v.9 no.3
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• pp.447-455
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• 2009

The power supply systems, which require low-voltage / high-current output has been changing from the conventional centralized power system to a distributed power system. The distributed power system consists of a bus converter and POL. The most important factor is the system stability in bus architecture design. The overlap between the output impedance of a bus converter input impedance of POL causes system instability and has been an actual problem. By increasing the bus capacitor, the system stability can be easily improved. However, due to limited space on the system board, the increasing of bus capacitors is impractical. An urgent solution of this issue is strongly desired. This paper presents the output impedance design for on-board distributed power system by means of three control schemes of a bus converter. The output impedance peak of the bus converter and the input impedance of the POL are analyzed and then conformed experimentally for stability criterion. Furthermore, the design process of each control schemes for system stability is proposed.

• Proceedings of the KIEE Conference
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• 1995.07b
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• pp.817-819
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• 1995

Applied by periodic Stimulating Currents in Bonhoeffer-Van der Pol(BVP) model, chaotic and periodic phenomena occured at specific conditions. The conditions of the chaotic motion in BVP comprised 0.7182< $A_{1}$ <0.792 and 1.09< $A_{1}$ <1.302 proved by the analysis of phase plane, bifurcation diagram, and lyapunov exponent. To control the chaotic motion, two methods were suggested by the first used the amplitude parameter $A_{1}$,$A_{1}={\varepsilon}((x-x_{s})-(y-y_{s}))$ and the second used the temperature parameter c, c=c$(1+ {\eta}cos{\Omega}t)$ which the values of $\eta$, ${\Omega}$ varied respectlvly, and $x_{s}$, $y_{s}$ are the periodic signal. As a result of simulating these methods, the chaotic phenomena was controlled with the periodic motion of periodisity. The feasibilities of the chaotic and the periodic phenomena were analysed by phase plane and lyapunov exponent.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.34 no.2
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• pp.195-206
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• 2016

The remote sensing technique using SAR data have many advantages when applied to the disaster site due to its wide coverage and all-weather acquisition availability. Although a single-pol (polarimetric) SAR image cannot represent the land surface better than a quad-pol SAR image can, single-pol SAR data are worth using for disaster-induced change detection. In this paper, an automatic change detection method based on a mixture of GGDs (generalized Gaussian distribution) is proposed, and usability of the textural features and intensity is evaluated by using the proposed method. Three ALOS/PALSAR images were used in the experiments, and the study site was Norita City, which was affected by the 2011 Tohoku earthquake. The experiment results showed that the proposed automatic change detection method is practical for disaster sites where the large areas change. The intensity information is useful for detecting disaster-induced changes with a 68.3% g-mean, but the texture information is not. The autocorrelation and correlation show the interesting implication that they tend not to extract agricultural areas in the change detection map. Therefore, the final tsunami-induced change map is produced by the combination of three maps: one is derived from the intensity information and used as an initial map, and the others are derived from the textural information and used as auxiliary data.

• Journal of The Korean Astronomical Society
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• v.55 no.4
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• pp.87-97
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• 2022

Polarimetric measurements of the lunar surface from lunar orbit soon will be available via Wide-Field Polarimetric Camera (PolCam) onboard the Korea Pathfinder Lunar Orbiter (KPLO), which is planned to be launched in mid 2022. To provide calibration data for the PolCam, we are conducting speckle polarimetric measurements of the nearside of the Moon from the Earth's ground. It appears that speckle imaging of the Moon for scientific purposes has not been attempted before, and there is need for a procedure to create a "lucky image" from a number of observed speckle images. As a first step of obtaining calibration data for the PolCam from the ground, we search for the best sharpness measure for lunar surfaces. We then calculate the minimum number of speckle images and the number of images to be shift-and-added for higher resolution (sharpness) and signal-to-noise ratio.