• Journal of Aquaculture
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• v.8 no.3
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• pp.149-157
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• 1995

In order to obtain the basic knowledges concerned to the semen preservation of aquacultural fishes, studies on the physical and chemical properties of semen, and sperm motility with the different osmotic pressures making by adding $Na^+,\;K^+,\; Mg^{++},\;and\;Ca^{++}$ to artificial seawater (ASW) were conducted in black seabream, Acanthopagrus schlegeli. Average semen volume per fish in one strip was 1.97ml and sperm concentration was $2.33\pm1.30\times10^{10}$ sperm/ml. Spermatocrit and pH of semen were $90.6\pm5.0\;and\;8.3\pm0.1$, respectively, Osmotic pressures of rearing seawater, seminal fluid and plasma were $939\pm24,382\pm70\;and\;342\pm77$ mOsm/l, and $Na^+,\;K^+$ and $Cl^-$ concentrations of seminal fluid were $169.5\pm4.5,\;4.9\pm2.2,\;156.0\pm2.0\;mM/l$, respectively. When semen were diluted by using $Na^+,\;K^+,\;Mg^{++}\;and\;Ca^{++}$ free ASW, only $Na^+$ free ASW had no sperm motility. As raising osmotic pressure graduary by addition of 1M NaCl to the $Na^+$ free ASW, spermatozoa showed the high motilities in 457-1128 mOsm/l, but the low motilities in 1398-1736 mOsm/l. In the case of same treatments with 1M of KCl, $MgC1_2\;and\;CaC1_2$ to the $K^+,\;Mg^{++}\;and\;Ca^{++}$ free ASW, spermatozoa revealed the high motilities in $904\~1434,\;818\~1175\;and\;956\~1343$ mOsm/l, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.5
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• pp.761-768
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• 2021

This study was carried out to identify an effective method to acclimate low-salinity (4 psu) bred Litopenaeus vannamei (mean body weight 16±3.3 g) to sea water. The fast acclimation group (FA) was directly exposed to filtered sea water (32 psu) while the slow acclimation group (SA) was exposed to a slow increase in salinity. Shrimps were sampled at 0, 1, 3, 6, 12, 24 and 48 h for plasma analyses. The plasma components between experimental groups did not show significant differences. The hemolymph osmolality (HO) in FA increased significantly after 1 h (P<0.05), while in SA it started to increase slowly only at 24 h and reached a similar level to that of FA at 48 h. The levels of Na⁺ and Mg²⁺ ions were significantly different between the two treatments (P<0.05), but there was no significant difference in Ca²⁺ ion levels. We found that the different methods of acclimation of L. vannamei to sea water do not affect the plasma components significantly, but lead to changes in the HO and ion levels, it is considerable to acclimate gradually for at least two days.

• Journal of Animal Science and Technology
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• v.62 no.1
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• pp.58-63
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• 2020

The pre-treatment condition affects on the element analysis of inductively coupled plasma (ICP). In this study the pre-treatment condition of ICP has been studied to quantify elements in dog's hair. The hair samples were collected from twelve female Beagles by clipping them into 1 or 2 cm at the back neck. The qualitative and quantitative analysis of elements in hairs were performed by using ICP. By ICP nine elements were qualitatively detected and quantitatively analyzed (Ca, Cu, Fe, K, Mg, Na, P, Se, Zn). The measured amounts of elements were compared between 3 step and 2 step procedures which were with and without the acetone based washing step. The quantitative analysis showed that the concentrations of K, Na, P, and Se were significantly decreased in hair samples with acetone-based washing (p < 0.005 or 0.001) unlike those without the acetone-based washing. It implied that some minerals are lost by the acetone based washing during the sample preparation step. Therefore, the acetone based washing process is not suitable for quantifying elements in dog's hair. In addition, the results of qualitative and quantitative analysis were compared. Although there was a difference in absolute values of elemental contents in hair, the results of qualitative and quantitative analysis were significantly correlated each other. This finding suggested that the results of qualitative analysis can be used to monitor elemental contents in dog's hair.

• Journal of the East Asian Society of Dietary Life
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• v.13 no.4
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• pp.326-333
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• 2003

The purpose of this study was to investigate the effect of various cooking methods(roasted, salad, deep-fried seasoned-roasted and commercial laver) on mineral contents, color and sensory evaluation of laver. The contents of mineral of dried laver by various cooking methods were analyzed using the Inductively Coupled Plasma(ICP) system. The results were summarized as follows : The content of crude protein, moisture, ash and crude fat in dried laver were 35.1％, 10.6％, 9.7％ and 0.8％, respectively. Among the minerals of dried laver, the content of poassium was the highest (2268.0mg/100g d.w.) and those of calcium and iron were comparatively high (495.1mg/100g, 13.5mg/100g). Ca/P ratio of dried laver was about 1:1 levels. Among various laver dishes, the total mineral content was the highest in the roasted laver, but low in the deep-fried laver. Among color values by cooking methods, "L(lightness)" and " - a(greenness)" values were the highest in the roasted laver, and "b(yellowness)" was the highest in the deep-fried laver. The seasoned-roasted laver was highly scored by the sensory evaluation.

• Journal of Nutrition and Health
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• v.25 no.6
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• pp.511-517
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• 1992

The hypoglycemic effects of Korean wild vegetables : Capsella bursa-pastoris Medicus(CBM) Commelina communis L. (CCL) Calystegics japonica Choisy(CJC) Discorea japonica Thunb(DJT) and Persicaria perfolidata Gross(PPG) in diabetic rats were determined. Sixty male Sprague-Dawley diabetic rats(130~180g) induced by the streptozotocin (45mg/kg) injection into the tail vein were fed either a control or experimental diets for four weeks. The plasma levels of glucose and cholesterol were measured. The urinary glucose levels were monitored. Crude fiber mineral(Ca, Fe, Zn and Cr) and ascorbic acid contents of the wild vegetables were analyzed. The extents of blood glucose decrement in CCL. DJT or PPG fed rats were greater than that in the control group. This extents of decrement in CBM or CJC fed rats were not significan-tly different from that in the control group. The urinary glucose was shown to be negative to Band reagent strip in CCL or DJT group at the 4th week. The plasma cholesterol levels of all the groups including control group were not essentially different. It is suggested that the intakes of CCL or DJT could be useful for prevetive and therapeutic approches to alleviate the hyperglycemic status in diabetes mellitus.

• The Korean Journal of Mycology
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• v.44 no.1
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• pp.23-30
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• 2016

This study was conducted for comparison of ingredients, phytochemical compounds and antioxidant activity of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do, and Jeolla-do. Three contents of Wofiporia extensa were analyzed as oxygen (46~48%), carbon (38~39%), hydrogen (6.05~6.1%) and nitrogen (0.17~0.21%). The mineral contents of 50% ethanol Wofiporia extensa extracts were measured as sulfur (S) 145~149 ppm, Magnesium (Mg) 69~72 ppm, phosphorus (P) 122~154 ppm and calcium (Ca) 210.61~509.98 ppm. Wofiporia extensa from Gyeongsang-do (509.98 ppm) contained a significantly higher quantity of Ca than that from Gangwon-do (210.62 ppm) and Jeolla-do (223.88 ppm). In the gas chromatograph-mass spectrometry (GC-MS) analysis, oleic acid was identified in three 50% ethanol Wofiporia extensa extracts. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay for antioxidant activity, the $IC_{50}$ values of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do and Jeolla-do were calculated as 2.966 mg/mL, 23.03 mg/mL, and 4.16 mg/mL and 3.521 mg/mL, 12.17 mg/mL, and 7.40 mg/mL. In the ferric reducing antioxidant power (FRAP) assay, the $IC_{50}$ values of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do, Jeolla-do were 6.585 mg/mL, 19.06 mg/mL, and 18.97 mg/mL, respectively. In summary, Wofiporia extensa cultured in Gangwon-do had stronger antioxidant activity and higher concentration of oleic acid than that of Geyongsang-do and Jeolla-do. However, Wofiporia extensa cultured in Geyongsang-do contained a much higher concentration of Ca than that of Gangwon-do and Jeolla-do.

• Journal of Animal Science and Technology
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• v.58 no.12
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• pp.42.1-42.8
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• 2016

Background: Deficiency of macro and micro-minerals in the ration of dairy cows adversely affects growth, milk production and reproduction efficiency. It is essential to examine mineral concentrations in feeds offered to dairy cows in practical farms. Methods: Two villages from each taluka were selected at random for taking representative samples of feeds, forages and hair. Within the village, help was sought from village milk producers and district animal husbandry officer for identification of 4 to 5 farmers and collection of representative samples. All the samples were processed and analyzed for chemical composition as well as major macro and micro-minerals, using Inductively Coupled Plasma-Optical Emission Spectrometer. Results: Ca content in wheat straw (0.29%), crushed maize (0.02%) and wheat bran (0.12%) was found to be below the critical level (0.30%). The P content in concentrate ingredients was high (0.26-0.96%), but low in dry roughages (0.06-0.12%). Cereal straws (0.14%) and grains (0.12%) were deficient in Mg. Feeds and forages were found to be adequate in K (1.50%). Cereals straws were found to be deficient in S (0.11%). Greens were good source of Cu (12.02 ppm). Wheat straw was found to be low in Zn (18 ppm), but high in Mn (225 ppm) and Fe (509 ppm). Local grasses and azolla green were found to be rich source of Co (>1.00 ppm). Se (0.63 ppm) was present in appreciable quantities in most of the feedstuffs. Conclusions: From the present study, it was apparent that the feeds and forages available in the state of Jharkhand may not meet the requirements for Ca, P, Mg, Cu, Zn and Co in order to sustain a milk production of ~10 kg/day. Therefore, it is necessary to supplement these deficient minerals through area specific mineral mixture in the ration of dairy cows for improving productivity and reproduction efficiency.

• Restorative Dentistry and Endodontics
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• v.33 no.4
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• pp.369-376
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• 2008

The aim of this study was to compare the compositions and cytotoxicity of white ProRoot MTA (white mineral trioxide aggregate) and 3 kinds of Portland cements. The elements, simple oxides and phase compositions of white MTA (WMTA), gray Portland cement (GPC), white Portland cement (WPC) and fast setting cement (FSC) were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES), X-ray fluorescence spectrometry (XRF) and X-ray diffractometry (XRD). Agar diffusion test was carried out to evaluate the cytotoxicity of WMTA and 3 kinds of Portland cements. The results showed that WMTA and WPC contained far less magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn) than GPC and FSC. FSC contained far more aluminum oxide ($Al_2O_3$) than WMTA, GPC, and WPC. WMTA, GPC, WPC and FSC were composed of main phases. such as tricalcicium silicate ($3CaO{\cdot}SiO_2$), dicalcium silicate ($2CaO{\cdot}SiO_2$), tricalcium aluminate ($3CaO{\cdot}Al_2O_3$), and tetracalcium aluminoferrite ($4CaO{\cdot}Al_2O_3{\cdot}Fe_2O_3$). The significance of the differences in cellular response between WMTA, GPC, WPC and FSC was statistically analyzed by Kruskal-Wallis Exact test with Bonferroni' s correction. The result showed no statistically significant difference (p > 0.05). WMTA, GPC, WPC and FSC showed similar compositions. However there were notable differences in the content of minor elements. such as aluminum (Al), magnesium, iron, manganese, and zinc. These differences might influence the physical properties of cements.

• Food Science and Preservation
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• v.4 no.3
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• pp.317-321
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• 1997

The mineral components of the Gastrodia elata Blum were determined by inductively coupled plasma-atomic emission spectrophtometry. The mineral component contents of the Gastrodia elata Blum were greater in the order of Fe < Na < Mg < Ca < P < K. The antioxidative effects of the Gastrodia elata Blum extracts on refined perilla oil were investigated by the rancimat method, peroxide value and TBA value. The antioxidative effects of Gastrodia elata Blum added to the refined perilla oil was increased with dose-dependent fashion in concentration of Gastrodia elata Blum extracts. The peroxide value and TBA value for their antioxidation stability were also lower than that of control. Antioxidation stability was increased in a dose-dependent manner.

• Journal of Environmental Health Sciences
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• v.16 no.2
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• pp.113-119
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• 1990

Many studies on the analysis of cations in blood have been reported. However, no suitable method for the pretreatment of blood for the determination of cations by Ion Chromotography. As a result, pretreatment method that the membrane filtration of plasma a diluted 1 to 100 fold acidified pH 3.5 was found to be the most suitable. The recoveris of monovalent cations in blood were yield 101%(Na$^{+}$). 102%(NH$^{+}_{4}$) and 101%(K$^{+}$) Determinations of divalent cations(Mg and Ca ions) in blood by Ion chromatography were summarized as followed conditions Separator Column : CS$_{3}$. Suppressor Column : CMMS. Eluent conen : 25m M-HCl/2mM-Histidine. Regenerant conen: 40mM-Ba(OH)$_{2}$.