• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.105-105
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• 2019

Dendropanax morbifera H.Lev is an evergreen tree that lives in subtropical climates. About 75 species of D. morbifera live in East Asia, but only one species live in Korea(Korean dendropanax). D. morbifera belonging to Aralicacae family 98% of D. morbifera are distributed in Jeollanam-do in the Korea and the grow wild in Gyeongsangnam-do and Jeju Island. The harvest time and usage of D. morbifera were recorded in traditional medicinal books. The roots and stems of D. morbifera had been used for traditional medicine to treat migraine, menstrual irregularity and skin disease. And D. morbifera leaves are contain flavonoids and polyacetylene compounds. In this study, we were investigated the physiological activity of D. morbifera by different areas collected at the same time, and compared to characteristics of plants. D. morbifera collected from Jeollanam-do (Goheung-gun), Gyeongsangnam-do (Namhae-gun) and Jeju Island, and dried at $50^{\circ}C$ for three days. We used dried D. morbifera powder for antioxidant tests. Each sample was extracted with hot water under the same conditions. The contents of total polyphenols and total flavonoids from D. morbifera were identified. Also, we performed to DPPH radical scavenging activity, ABTS cation radical scavenging activity and Superoxide anion scavenging activity efficacy for antioxidant activity determination. The contents of total polyphenols and total flavonoids in hot water extract of D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do were higher than Jeju. However, D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do showed no significant difference those content of total polyphenols and total flavonoids. And the antioxidant capacity was showed the similar patterns in antioxidant activity.

• Journal of Ginseng Research
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• 제35권3호
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• pp.368-374
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• 2011

American ginseng (Panax quinquefolius L.) is grown in some regions of the USA and Canada and marketed for its health promoting attributes. While cultivation of this plant species has taken place in North America for over 100 years, there are many challenges that need to be addressed. In this article, the current production method used by growers is described and the challenges and opportunities for research on this valuable plant are discussed. These include studies on pharmacological activity, genetic diversity within the species, genetic improvement of currently grown plants, molecular characterization of gene expression, and management of diseases affecting plant productivity. The current research developments in these areas are reviewed and areas requiring further work are summarized. Additional research should shed light on the nature of the bioactive compounds and their clinical effects, and the molecular basis of active ingredient biosynthesis, and provide more uniform genetic material as well as improved plant growth, and potentially reduce losses due to pathogens.

• Natural Product Sciences
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• 제16권1호
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• pp.6-9
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• 2010

In continuing our search for biologically active compounds from Korean Compositae medicinal plants, we investigated the constituents of the aerial parts of Lactuca indica L. and isolated a phenylpropanoid derivative from its MeOH extract. The chemical structure was characterized by spectroscopic methods, including 1D and 2D NMR to be di-E-caffeoyl-meso-tartaric acid (1). Compound 1 was isolated for the first time from this plant. In this paper, we suggest that the NMR assignment at C-2 of (+)-taraxafolin-B should be corrected. In the human HBV-transfected liver cell line HepG2.2.15, the compound 1 effectively reduced HBV DNA level in the release of mature HBV particles from HepG2.2.15 cultivation.

• Journal of Microbiology and Biotechnology
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• 제28권12호
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• pp.2057-2063
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• 2018

Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene Lcc9 from Laccaria bicolor was chemically synthesized and optimized to heterogeneous expression in Pichia pastoris and Arabidopsis thaliana. The properties of recombinant laccase expressed by P. pastoris were investigated. The laccase activity was optimal at 3.6 pH and $40^{\circ}C$. It exhibited $K_m$ and $V_{max}$ values of $0.565mmol\;l^{-1}$ and $1.51{\mu}mol\;l^{-1}\;min^{-1}$ for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic P. pastoris or A. thaliana could decolorize crystal violet. These results indicated that L. bicolor laccase gene may be transgenically exploited in fungi or plants for dye decolorization.

• 인간식물환경학회지
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• 제21권6호
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• pp.461-471
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• 2018

The role of urban agriculture should not be limited as a small-scale crop cultivation activity as it was, but it has to be considered as an entire process of a agriculture activity for the restoration of the city community. This study is based on the assumption that there has been a significant change in urban lifestyle and urban farming preference, considering the overall improvement in standard of living after implementation of five day work week system. It was conducted for urban citizens who were interested in actual urban agriculture for ages 19 and over who visited the Korea Urban Agriculture Exhibition in 2018. Only 115 valid samples were used for the empirical analysis. To analyze the demographic characteristics and effects of urban agriculture, frequency analysis and descriptive statistics were conducted. In order to analyze the reliability and validity of the measurement variables of the effect, the variables that deteriorate the validity were removed and 15 variables of the urban agricultural effect were selected. According to the result of factor analysis, three factors were extracted as follows: socially sustainable effect, negative effect and economically sustainable effect. In order to examine the effects of urban agriculture depending on income level, the one-way ANOVA, which is a statistical technique for verifying differences in the sample means, was performed. The psychological stability of people, the recovery of humanity through communion with nature, and the vitalization of agriculture linked with local agriculture had significant correlations with income level. The negative effect showed no significant correlation with income level. The improvement of the local environment was found to have an impact in relation with income level. We expect that there will be more studies on policies for the new types and models of urban agriculture in order to make it easier for urban citizens to approach it.

• 식물조직배양학회지
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• 제26권3호
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• pp.163-169
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• 1999

우리나라의 주요 개량 포플러의 하나인 현사시 3호를 대상으로 Arobacterium tumefaciens MP 90/PAT을 이용하여 비선택성 제초제인 Basta에 내성을 갖는 형질전환체를 획득하고자 본 실험을 수행하였다. 기내 배양된 엽절편을 재료로 균주에 10분간 감염, 2일간 공조 배양 및 3주간 선발배지에서 배양으로 kanamycin에 내성을 갖는 잠정적인 형질전환 캘러스를 유도하였다. 선발된 캘러스로부터 배양 4주 후 줄기를 유도하였으며, 증식된 줄기는 발근시켜 완전한 형질전환체를 얻었다. 형질전환체는 PCR기법 및 Southern blot 분석으로 PAT 유전자 전이를 확인하고. 유전자의 발현은 GUS 유전자의 발색 반응으로 확인하였다. 형질전환체는 폿트로 이식하여 4주간 생장시킨 후 제초제 Basta로 살포한 결과 대조구 식물체는 고사되었으나, 형질전환체는 정상적인 생육이 가능하였다

• 한국환경농학회지
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• 제14권2호
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• pp.186-201
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• 1995

Pyrethroid계 살비살충제 acrinathrin 토양잔류물의 행적을 구명하기 위하여 [$^{14}C$]acrinathrin의 신생 및 1개월간의 숙성잔류물을 함유한 2종의 토양이 담긴 특수 제작한 stainless steel pot에서 옥수수를 1개월간 재배하였다. 1개월의 숙성기간과 1개월의 옥수수 재배기간중 [$^{14}C$]acrinathrin이 무기화되어 발생된 $^{14}CO_2$의 양은 두 토양 모두 총처리 방사능의 각각 $23{\sim}24%$와 $24{\sim}33%$이었다. 1개월간 재배한 후 수확한 옥수수의 지상부와 지하부에 흡수이행된 방사능은 두 토양 모두에서 각각 총처리 방사능의 0.1%와 1% 미만인 반면 전체 방사능의 $65{\sim}80%$는 토양에 남아 있었다. [$^{14}C$]acrinathrin을 처리한 후 옥수수를 재배하기 전과 후 토양 추출액의 autoradiography와 GC-MS로 1종의 미확인 분해산물을 비롯하여 m/z 198, m/z 228 및 m/z 214의 분해산물을 검출하였고 비표지 acrinathrin을 처리하고 30일간 옥수수를 재배한 토양 추출액에서는 GC-MS로 m/z 225와 m/z 198의 분해산물을 확인하였다. 토양중에서 acrinathrin은 cyano group이 결합된 탄소 ($^{14}C$)에 인접한 ester 결합이 가수분해되어 3-phenoxybenzaldehyde cyanohydrin을 형성한 후 뒤이어 HCN이 제거되어 3-phenoxybenzaldehyde를 생성하고 이것이 다시 3-phenoxybenzoic acid로 산화된 후 decarboxylation에 의하여 $^{14}CO_2$가 방출되는 것으로 판단되었다. 1개월간 옥수수를 재배한 토양과 1개월간 숙성한 토양의 acetone에 의한 추출율은 두 토양 모두에서 총처리 방사능의 31% 미만이었으며, 토양 추출액 방사능은 95% 이상이 유기상에 분배되었다. 따라서 acrinathrin은 토양중에서 분해가 신속하고 토양구성성분에 쉽게 흡착 또는 결합되어 작물에 흡수이행되지 않는 것으로 판단되었다.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.219-223
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• 2009

Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress.

• Preventive Nutrition and Food Science
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• 제10권2호
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• pp.172-178
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• 2005

Dandelions have been reported to have medicinal properties and bioactive components that impact human health. However, the precise biological properties of dandelions and the parts of the plants possessing bioactive components remain uncertain. In this study, we evaluated 3 different types of dandelions based on their cultivation origin (Songpa, Uiryung, and native Uiryung types) as well as their 4 different plant parts (leaf, flower, root, skin). Each sample was extracted with $80\%$ methanol and then compared for the biological activities (anti-oxidative, immune cell proliferative and tumor cell growth inhibitory activities). All 3 types of dandelions possessed a degree of biological functions including the hydroxyl radical scavenger activity, immune cell proliferative activity and tumor cell growth inhibitory activity. However, there was no significant difference in these activities between the 3 dandelion types. Leaves of all three dandelion types showed the highest levels of all biological activities. To a lesser degree, the flower and root parts displayed biological activities. In the skin parts, anti-oxidative activity was also detected only at higher doses of dandelion extracts. Heating the dandelion leaf extract did not affect the biological activity, suggesting a heat-stable nature of the biological compounds. Taken together, these collective data suggest that dandelions, in particular their leaves, possess a high concentration of heat-resistant biological compounds, which are responsible for anti-oxidative, immune cell proliferative and tumor cell growth-inhibitory activities.

• 한국유기농업학회지
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• 제14권2호
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• pp.205-217
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• 2006

친환경 유기 논 농업 토양 관리 기술을 개발하기 위하여 화학비료대신 유기물원인 볏짚, 퇴비, 헤어리베치, 그리고 자재는 인광석, 석회고토분말, Ash를 사용하여 유기벼 재배 및 유기 논 농업 토양 관리 기술에 대한 효과 검정을 수행한 결과는 다음과 같다. 1. 수도의 시기별 생육상황은 삼요소구에 비하여 유기물원 볏짚과 퇴비처리에서 초장 및 경수가 적은 경향이고, 두과작물인 헤어리베치 시용구는 삼요소구와 거의 같거나 비슷한 경향을 보였다. 2. 토양산화환원(Eh)의 변화를 보면 처리에 상관없이 5월 30일부터 감소하기 시작하여 6월 9일에는 급속하여 낮아졌는데 (-150mv에서, -190mv) 이는 온도상승으로 인한 미생물의 활성이 증가한 것으로 생각되어진다. 3. 수량구성요소 및 수량에서 수수는 유기물원의 연용에는 차이가 나지 않았으며, 삼요구와는 약간의 차이를 보였으나, 볏짚가 퇴비처리가 헤어리 처리보다 수수가 적은 경향을 보였고, 등숙율은 삼요소구에 비하여 유기물원 처리가 공히 높은 경향을 보였고 처리간에 유의성은 인정되지 않았고, 천립중에 있어서도 유기물원 시용 여부에는 차이가 나지 않았고 삼요소구와도 차이가 없었다. 수량에 있어서 삼요소구 511kg/10a에 비해 토비시용은 98%, 퇴비99%, 헤어리베치 103%, 삼요소+퇴비 101%로 유의성은 인정되지 않았다. 4. 유기물원 시용으로 시험 후 토양의 화학성을 보면 유효인산과 치환성가리, 치환성 칼슘 등의 함량이 증가하는 반면, 공극율 및 용적밀도가 볏짚>헤어리베치>퇴비>퇴비+삼요소>삼요소>무비 순으로 토양 물리성이 양호하였다. 5. 이상의 결과를 볼 때 유기 논 농업 토양 기술개발의 관리 측면에서 일반 관행농업보다는 수량은 볏짚과 퇴비에서 감소하나 두과작물인 헤어리베치를 재배하여 시용하면 토양의 화학성과 물리성이 양호하고, 생육 및 수량도 일반관행농법과 거의 비슷하므로 유기벼 재배 및 논토양관리에서는 활용 가능성이 있는 것으로 나타났다.