• The Plant Pathology Journal
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• v.35 no.5
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• pp.498-507
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• 2019

Odontoglossum ringspot virus (ORSV) is a member of the genus Tobamovirus. It is one of the most prevalent viruses infecting orchids worldwide. Earlier studies reported the genetic variability of ORSV isolates from Korea and China. However, the evolutionary rate, timescale, and phylogeographical analyses of ORSV were unclear. Twenty-one coat protein (CP) gene sequences of ORSV were determined in this study, and used them together with 145 CP sequences obtained from GenBank to infer the genetic diversities, evolutionary rate, timescale and migration of ORSV populations. Evolutionary rate of ORSV populations was $1.25{\times}10^{-3}nucleotides/site/y$. The most recent common ancestors came from 30 year ago (95% confidence intervals, 26-40). Based on CP gene, ORSV migrated from mainland China and South Korea to Taiwan island, Germany, Australia, Singapore, and Indonesia, and it also circulated within east Asia. Our study is the first attempt to evaluate the evolutionary rates, timescales and migration dynamics of ORSV.

• The Plant Pathology Journal
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• v.35 no.3
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• pp.200-207
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• 2019

Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb1 is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like (PFT) gene was recently identified as an underlying gene for Fhb1 resistance. In this study, we developed and validated a PFT-based Kompetitive allele specific PCR (KASP) marker for Fhb1. The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering results were compared with gelbased gene specific markers and the widely used linked STS marker, UMN10. Eight disagreements were found between PFT_KASP and UMN10 assays among the tested lines. Based on the genotyping and sequencing of genes in the Fhb1 region, these genotypes were found to be common with a previously characterized susceptible haplotype. Therefore, our results indicate that PFT_KASP is a perfect diagnostic marker for Fhb1 and would be a valuable tool for introgression and pyramiding of FHB resistance in wheat cultivars.

• Journal of Ecology and Environment
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• v.46 no.2
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• pp.126-135
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• 2022

Background: Pollinators are important ecological elements due to their role in the maintenance of ecosystem health, wild plant reproduction, crop production and food security. The pollinator-plant interaction supports the preservation of plant and animal populations and it also improves the yield in pollination dependent crops. Having knowledge about the plant-pollinator interaction is necessary for development of pesticide risk assessment of pollinators and conservation of endangering species. Results: Traditional methods to discover the relatedness of insects and plants are based on tracing the visiting pollinators by field observations as well as palynology. These methods are time-consuming and needs expert taxonomists to identify different groups of pollinators such as insects or identify flowering plants through palynology. With pace of technology, using molecular methods become popular in identification and classification of organisms. DNA metabarcoding, which is the combination of DNA barcoding and high throughput sequencing, can be applied as an alternative method in identification of mixed origin environmental samples such as pollen loads attached to the body of insects and has been used in DNA-based discovery of plant-pollinator relationship. Conclusions: DNA metabarcoding is practical for plant-pollinator studies, however, lack of reference sequence in online databases, taxonomic resolution, universality of primers are the most crucial limitations. Using multiple molecular markers is preferable due to the limitations of developed universal primers, which improves taxa richness and taxonomic resolution of the studied community.

• Coupled systems mechanics
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• v.4 no.2
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• pp.157-172
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• 2015

Control of potable water treatment plant (PWTP) is nowadays based on experience. The aim of this article is to show that model based control of treatment process is more efficient than process operation based on experience. Stimela environment is used for modeling of processes of potable water treatment. Application of the model was conducted on PWTP "Crkvice" in Zenica (BiH). This plant has used conventional rapid sand filters. By effective application of the model it is determined the optimal filter run time for different input turbidity of raw water. This results in the possibility of reducing the consumption of backwashing water, lower costs for its pumping and reducing the amount of coagulants. In the existing practice, based on experience, these benefits are not used.

• Journal of the Korean Society of Systems Engineering
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• v.14 no.2
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• pp.49-57
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• 2018

The main objective of this paper is come up with methodology approach for FPGA-based system in verification and validation lifecycle regarding software reliability using system engineering approach. The steps of both reverse engineering and re-engineering are carried out to implement an FPGA-based of safety critical system in Nuclear Power Plant. The reverse engineering methodology is applied to elicit the requirements of the system as well as gain understanding of the current life cycle and V&V activities of FPGA based-system. The re-engineering method is carried out to get a new methodology approach of software reliability, particularly Software Reliability Growth Model. For measure the software reliability of a given FPGA-based system, the following steps are executed as; requirements definition and measurement, evaluation of candidate reliability model, and the validation of the selected system. As conclusion, a new methodology approach for software reliability measurement using software reliability growth model is developed.

• Journal of the Korean Society of Safety
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• v.22 no.6
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• pp.35-40
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• 2007

As a way of improving the safety of the chemical facilities, the risk based-inspection(RBI) was executed for the facilities of the applied petrochemical plant using KS-RBI Ver. 3.0 program developed based on the API-581 based resource document(BRD). From an evaluation result of KS-RBI program, we could find the evaluation of the process safety management(PSM) for the applied plant, risk of the applied process, risk of static facilities and pipes, and the demage mechanism of the facilities. Also, we could suggest a proper inspection plan(frequency and method of inspection) using the calculated risk and the status of the facilities. Therefore, the applied plant could be achieved a reduced inspection cost by an extension of inspection frequency, improved productivity, improved reliability of the facilities, and a computerized history management.

• Proceedings of the KIEE Conference
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• 1997.07a
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• pp.127-129
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• 1997

In this paper, an active magnetic bearing-based motor-generator(M-G) system is designed and controlled by using DSP devices. Several experiments including start-up test, impulse test, whirl test, and generator load test are conducted using digital PID algorithm and AC power of about 58Hz, 100V, 0.8A can be generated from the M-G set.

• Journal of the Korean Society for Nondestructive Testing
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• v.28 no.6
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• pp.469-476
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• 2008

Condition based maintenance(CBM) for the preventive diagnosis of important equipments related to safety or accident in power plant is essential by using the suitable methods based on actual power plant conditions. To improve the reliability and accuracy of the measured value at the minute leak situation, and also to monitor continuously internal leak condition of power plant valve, the development of a diagnosis and monitoring technique using multi-measuring method should be performed urgently. This study was conducted to estimate the feasibility of multi-measuring method using three different methods such as acoustic emission(AE) method, thermal image measurement and temperature difference$({\Delta}T)$ measurement that are applicable to internal leak diagnosis for the power plant valve. From the experimental results, it was suggested that the multi-measuring method could be an effective way to precisely diagnose and evaluate internal leak situation of valve.

• IE interfaces
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• v.23 no.3
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• pp.213-220
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• 2010

As the market becomes increasingly globalized and competition among companies increases in severity, various specialized organizations are participating across the process plant lifecycle, including the stages of design, construction, operation and maintenance, and dismantlement, in order to ensure efficiency and elevate competitiveness. In this regard, it is an important technical issue to develop services or information systems for sharing process plant data among participating organizations. ISO 15926 is an international standard for integration of lifecycle data for process plants including oil and gas facilities. ISO 15926 Part 7, a part of the ISO 15926 standard, specifies an implementation method called a facade that uses Web Services and ontology technologies for constructing plant data repositories and related services, with the aim of sharing lifecycle data of process plants. This paper discusses the ISO 15926-based prototype facade implemented for storing equipment data of nuclear power plants and servicing the data to interested organizations.

• International Journal of Safety
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• v.11 no.1
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• pp.26-32
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• 2012

This paper introduces a feasibility evaluation method for prognosis systems based on an empirical model in nuclear power plants. By exploiting the dynamical signature characterized by abnormal phenomena, the prognosis technique can be applied to detect the plant abnormal states prior to an unexpected plant trip. Early $operator^{\circ}{\emptyset}s$ awareness can extend available time for operation action; therefore, unexpected plant trip and time-consuming maintenance can be reduced. For the practical application in nuclear power plant, it is important not only to enhance the advantages of prognosis systems, but also to quantify the negative impact in prognosis, e.g., uncertainty. In order to apply these prognosis systems to real nuclear power plants, it is necessary to conduct a feasibility evaluation; the evaluation consists of 4 steps (: the development of an evaluation method, the development of selection criteria for the abnormal state, acquisition and signal processing, and an evaluation experiment). In this paper, we introduce the feasibility evaluation method and propose further study points for applying prognosis systems from KHNP's experiences in testing some prognosis technologies available in the market.