• 한국식물병리학회지
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• 제3권4호
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• pp.291-298
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• 1987

모자이크, 엽맥록대, 퇴록반점 등의 병징을 나타내고 있는 동부 (Vigna sesquipedalis)를 진주에서 채집하였다. 전자현미경에 의해 2종의 바이러스가 분리되어 이들의 기주범위, 조즙액중의 부활성화한계, 감염엽세포에 있어서 입자의 소재양식에 의하여 2종의 바이러스를 동정하였다. Blackeye cowpea mosaic virus (BICMV). 이 바이러스는 즙액접종에 의해 2과 7종의 검정식물에 감염이 확인되었다. 그러나 전신감염은 두과식물에 한했다. 내열성은 $50-60^{\circ}C$, 내희석성은 $10^{-4}-10^{-5}$, 내보존성은 1-6일이었다. 바이러스 입자의 형태는 길이 약 750nm의 사상형이다. 병엽 절편의 전자현미경적 관찰에서는 엽육세포의 세포질에서 사상 입자와 풍차상 및 환상의 봉입체가 확인되었다. Cucumber mosaic virus(CM V), 이 바이러스는 즙액접종에 의해 3과 9종의 검정식물에 감염이 확인되었다. 다른 CM V계와 달리 두과식물에도 전신감염을 했다. 이 바이러스 임자의 형태는 직경약 30nm의 입자이다. 감염엽의 전자현미경적 관찰에서는 엽육세포의 세포질 및 액포내에서 바이러스 입자의 응집상이 확인되었다.

• 식물병연구
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• 제28권2호
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• pp.82-91
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• 2022

최근에는 작물의 유도 저항성을 이용한 항바이러스제 개발에 관한 많은 연구가 수행되고 있으나 실제 농업현장에 널리 보급되지 못하고 있는 실정이다. 본 실험은 시설토마토 재배현장에 외생 살리실산, 키토산, 유제놀 처리에 따른 토마토황화잎말림바이러스(Tomato yellow leaf curl virus, TYLCV) 감염억제효과를 검증하고자 수행되었다. 실내검정에서 TYLCV에 감수성 품종인 '슈퍼도태랑'은 항바이러스제 처리 후 TYLCV에 감염된지 12일 후 VP (virus infected control plants)에서 바이러스 증상이 나타나기 시작했다. 접종 32일 후 TYLCV 발병도는 VP에서 98.8%였고, SAT (2 mM salicylic acid)+VP, EGT (200 ㎍/ml eugenol)+VP에서는 각각 98.8%, 98.7%로 발병도가 높았으나, CHT (0.1% chitosan)+VP는 85.7%로 다른 처리들과 통계적으로 유의한 차이를 보였다. 그러나 TYLCV 농도는 CHT+VP에서 OD값이 0.3으로 오히려 가장 높게 나타났으며, 토마토의 초장, 지상부 및 지하부 생체중에서도 뚜렷한 효과를 보이지 않았다. 여름작형 시설재배지에서 도태랑 계열의 토마토품종 '도태랑솔라'를 사용하여 항바이러스 3종의 효과를 조사한 결과, 수확기에 모든 처리구에서 100.0%에 가까운 TYLCV 감염률은 나타냈으며, 수확량에도 처리간의 통계적 유의차가 인정되지는 않았다. 이와 대조적으로 Ty-1과 Ty-3a 유전자를 보유한 TYLCV에 내병성 품종인 'TY자이언츠'는 저항성 유묘검정의 전 조사기간 동안 바이러스 증상이 전혀 관찰되지 않았고, 식물체내 바이러스 농도도 무접종 수준이었다. 본 실험 결과 'TY자이언츠' 품종은 TYLCV 발생이 만연한 지역 및 재배시기에 감수성 품종을 대체할 수 있을 것으로 생각된다. 반면, 저항성 유도물질인 살리실산, 유제놀, 키토산의 항바이러스 효과는 입증되지 않았기 때문에, 아직 시설토마토 재배현장에 적용하기는 어려울 것으로 판단된다.

• The Plant Pathology Journal
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• 제34권5호
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• pp.435-444
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• 2018

Receptor-like proteins (RLPs) are involved in plant development and disease resistance. Only some of the RLPs in tomato (Solanum lycopersicum L.) have been functionally characterized though 176 genes encoding RLPs, which have been identified in the tomato genome. To further understand the role of RLPs in tomato, we performed genome-guided classification and transcriptome analysis of these genes. Phylogenic comparisons revealed that the tomato RLP members could be divided into eight subgroups and that the genes evolved independently compared to similar genes in Arabidopsis. Based on location and physical clustering analyses, we conclude that tomato RLPs likely expanded primarily through tandem duplication events. According to tissue specific RNA-seq data, 71 RLPs were expressed in at least one of the following tissues: root, leaf, bud, flower, or fruit. Several genes had expression patterns that were tissue specific. In addition, tomato RLP expression profiles after infection with different pathogens showed distinguish gene regulations according to disease induction and resistance response as well as infection by bacteria and virus. Notably, Some RLPs were highly and/or unique expressed in susceptible tomato to pathogen, suggesting that the RLP could be involved in disease response, possibly as a host-susceptibility factor. Our study could provide an important clues for further investigations into the function of tomato RLPs involved in developmental and response to pathogens.

• The Plant Pathology Journal
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• 제22권3호
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• pp.255-259
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• 2006

The pathogenicity to pear trees and other experimental hosts of the Apple stem grooving virus Korean isolate (ASGV-K) carried by a fungal vector, Talaromyces flavus was examined. ASGV-harboring T. flavus induced mild symptoms on virus-free pears. Symptom severity was intermediate between pears showing typical PBNLS and virus-free pears. Ten cultivars of Phaseolus vulgaris showed 35%-90% infectivity by direct infiltration into leaves and roots by ASGV-harboring T. flavus. Application of fungal cultures to soils showed 0%-70% infectivity depending on the P. vulgaris cultivar. Sap extracted from ASGV-infected Chenopodium quinoa induced similar symptoms on P. vulgaris at 25 days after inoculation. Similar symptoms were also detected on P. vulgaris which were inoculated with ASGV-harboring T.flavus. When healthy P. vulgaris leaves were challenged with sap extracted from P. vulgaris leaves infected with ASGV-harboring T. flavus, typical symptoms were observed. These data suggest that T. flavus mediates the transfer of ASGV to host plants.

• 식물병연구
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• 제8권4호
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• pp.207-214
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• 2002

대구시와 경상북도 지역에서 Tobacco rattle virus(TRV) 감염에 의한 증상으로 보이는 글라디올러스(Gladiolus hybridus), 크로커스(Crocus spp.), 수선화(Narcissus spp.)를 채집하였다. 톱니무늬(notch), 줄무늬(stripe), 변형 (malformation)등의 증상의 잎 조직을 시료로 하여 direct negative staining method(DN) 및 immunosorbent electron microscopy(ISEM)법에 의해 투과전자현미경으로 바이러스 입자를 관찰한 결과, 각각의 시료에서 긴 입자는 170~200 X 22nm, 짧은 입자는 40~114$\times$22nm 크기의 막대모양 입자가 다수 확인되었다. 글라디올러스에서 분리한 tobacco rattle virus(TRV-K)를 담배(Nicotiana tabacum)에 즙액 접종, 증식시킨 후, 정제하여 항혈청을 제작하였다. TRV-K 의 coat protein(CP)유전자를 특이적인 올리고뉴클레오타 이드 프라이머를 이용하여 역전사-중합효소연쇄반응(RT-PCR)으로 증폭시킨 후에 염기서열분석법으로 확인하였다. 그 결과 TRV-ORY의 CP와 99.5%의 상동성을 나타냈다.

• The Plant Pathology Journal
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• 제26권4호
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• pp.328-339
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• 2010

A tobamovirus, Ribgrass mosaic virus (RMV), was identified newly from chinese cabbage (Brassica campestris L. pekinensis) in Korea. Virus disease incidence of RMV on chinese cabbage was 37.9% in alpine area on August in 1993. RMV induced the symptoms of necrotic ring spots, necrotic streak on midrib and malformation. RMV, Ca1 and Ca3 isolate, could infect 35 species out of 45 plants including Chenopodium amaranticolor. Physical properties of RMV Ca1 isolate were very stable as 10.8 over for dilution end point, $95^{\circ}C$ for temperature inactivation point and 18 weeks for longevity in vitro. RMV had the soil transmission rate of 75.0% for the chinese cabbages, 'Chunhawang' and 'Seoul' cultivars. The purified virions of RMV had the typical ultraviolet absorption spectrum of maximum at 260 nm and minimum at 247 nm. RMV of Ca1 isolate was related serologically with antisera of Tobacco mosaic virus (TMV)-Cym, TMV-O and Pepper mottle virus, but not related with antiserum of Odontoglossum ring spot virus. coat protein gene of RMV-Ca1, sized 473 nucleotides, encoded 158 amino acid residues. Nucleotide identity of RMV-Ca1 CP gene was 96.4% with RMV-Shanghai (GenBank accession No. of AF185272) from China and 96.0% with RMV-Impatiens (GenBank accession No. of AM040974) from Germany. Identity of amino acids between RMV-Ca1 and the two RMV isolates was 96.8%. Specific three primers were selected for rapid and easy genetic detection of RMV using Virion Captured (VC)/RT-PCR method.

• 대한수의학회지
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• 제52권4호
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• pp.259-262
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• 2012

Classical swine fever (CSF) is a highly contagious disease among swine that has an important economic impact on worldwide. One clinical symptom of CSF is leukopenia, in particular lymphopenia, which is a characteristic event that occurs early in the course of CSF. Though lymphopenia associated with apoptosis, the pathogenic mechanism underlying the lymphopenia has not been well studied. To understand these mechanisms, we investigated the response of porcine B cell lines to infection with SW03, virulent strain isolated from swine tissue in Korea. This study demonstrated that SW03-infected L35 cell were induced apoptosis through the detection of activated caspase-3. In addition, SW03 infection leaded to alterations in pro-apoptotic, Bax, and anti-apoptotic, Bcl-xL proteins of Bcl-2 family. Our results would suggest that SW03-infected L35 cells induced apoptosis via intrinsic mitochondrial pathway.

• The Plant Pathology Journal
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• 제24권3호
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• pp.328-336
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• 2008

Out of various fungi and bacteria tested for inhibition of Tobacco mosaic virus(TMV) infection using Nicotiana tabacum cv. Xanthi-nc, a bacterial isolate JB-l, identified as Pseudomonas putida had a strong direct inhibitory activity against the TMV infection. Its systemic or indirect activity was also noted at more than a half level of the direct control efficacy. Disease severity was reduced significantly in the susceptible tobacco N. tabacum cv. NC 82 by the treatment of the bacterial culture filtrate, somewhat more by the pretreatment than by simultaneous treatment, probably by inhibiting the TMV transmission and translocation in the plants, showing negative serological, which responses in the viral detection by DAS-ELISA. TMV-inhibitory substances from P. putida JB-1 were water-soluble, stable to high temperature(even boiling), and to a wide range of pH. As proteinase K nullified their antiviral activity, the TMV inhibition activity of P. putida may be derived from proteinaceous materials. In electron microscopy, TMV particles treated with the JB-1 culture were shown to be shrunken with granule-like particles attached on them. All of these aspects suggest that P. putida JB-1 may be developed as a potential agent for the control of TMV.

• The Plant Pathology Journal
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• 제33권3호
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• pp.345-349
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• 2017

Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient tomato seedlings. Forty-one of 46 tomato seedlings tested were grafted successfully with single-leaflets infected with ToCV. Among them, 36 seedlings (87.8%) were systemically infected with ToCV and developed typical symptoms. Our results demonstrated that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of ToCV to the grafted seedlings.

• 식물병연구
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• 제7권3호
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• pp.170-174
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• 2001

시판고추 종자로부터 Tobamovirus를 검출하고 Tobamovirus 오염률을 조사하고자 gelatin particle agglutination test (GPAT)의 최적조건을 조사하였다. Gelatin 입자에 감작할 담배 모자이크 바이러스 고추 계통에 대한 ${\gamma}$-globulin G 의 최적농도는 100$\mu\textrm{g}$/$m\ell$이었고, GPAT의 바이러스 검출 감도는 효소면역항체법 및 dot immuno-binding assay와 유사하였으며, Tobamovirus 검출을 위한 종자시료의 최적 희석배수는 5배에서 25배였다. 그리고 최적화된 GPAT에 의한 국내시판 고추종자의 Tobamovirus 오염률은 평균 79.1%인 것으로 판명되었다.