• Title/Summary/Keyword: Plant propagation

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An Efficient Plant Regeneration System for Sorghum bicolor - a Valuable Major Cereal Crop

  • Baskaran P.;Jayabalan N.
    • Journal of Plant Biotechnology
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    • v.7 no.4
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    • pp.247-257
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    • 2005

  • An efficient, rapid and large-scale in vitro clonal propagation of agronomically important Indian cereal crop genotypes (NSH27 & K5) of Sorghum bicolor (L.) Moench. by enhanced shoot proliferation in shoot tip segments was designed. MS medium fortified with plant growth regulators and coconut water markedly influenced in vitro propagation of Sorghum bicolor. In vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), 5% coconut water and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of shoot tip segments on a similar medium enabled continuous production of more than 100 healthy shoots with similar frequency. When the healthy shoot clumps were cultured on MS medium fortified with 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), ${\alpha}$-naphthaleneacetic acid ($2.7\;{\mu}M$), ascorbic acid ($30.0\;{\mu}M$) and 5% coconut water, a rapid production of axillary and adventitious buds was developed after 8 wk culture. More than 300 shoots were produced 10 wk after culture. Rooting was highest (100%) on half strength MS medium containing 22.8 mM IAA. Micropropagated plants established in garden soil, farmyard soil and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics. These plants grew normally without showing any traits.

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ACTUAL STATE AND PRACTICAL USE OF THE FACTORY-STYLE PLANT PRODUCTION SYSTEM USING TISSUE CULTURE

  • Holdgate, D.P.;Zandvoort, E.A.
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 1996.05a
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    • pp.41-62
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    • 1996

  • Since 1966 tissue culture has been used as a tool for the production of disease indexed stocks from selected plants and their rapid (clonal) mass propagation through the procedure now referred to as micropropagation. The major advantages have been the rapid introduction of new plant cultivars, created within conventional and mutation breeding programmes, as healthy stock for beneficial distribution and the expansion of the world wide horticultural industry. (omitted)

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The study on the culture of Lycoris radiata HERB in Medicinal Plant II. The Effect of Shade net on Growth and Bulb Yield (약용식물인 석산의 재배학적 연구 II. 광강도가 석산의 생육 및 인경수량에 미치는 영향)

  • 이종일
    • Korean Journal of Plant Resources
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    • v.4 no.1
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    • pp.23-27
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    • 1991

  • This experiment was conducted to establish shade-method suited to on tile artificial propagation of Lycoris radiata HERB. The ligilt intensity in the native habitatwas 30 kux in March and 3.2 Klux from June to sept. The average light intensityin tile open area was 137 Klux in June. The plant height and number of Ieaves wasgood at the 35% to 55% shadeing net. Bloonling time of Lycoris radiata HERB was inthe last of Sept. Yeild of bulb was increased in 35% to 55% of shadeing net.

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