• 한국식물병리학회지
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• 제11권1호
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• pp.53-60
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• 1995

Inoculation with the compatible strain Ds 1 of Xanthomonas campestris pv. vesicatoria caused brownish ad water-soaked lesions, but incompatible strain Bv5-4a produced hypersensitive symptoms with local necrosis on tomato (cv. Kwangyang) leaves. Bacterial populations of the compatible strains Ds 1 propagated more greatly than the incompatible strain Bv5-4a at the frist onset, but no differences were observed 5 days after inoculation. The bacterial infection induced the synthesis and accumulation of soluble proteins in tomato leaves, especially in the incompatible interaction. Native-polyacrylamide gel electrophoresis distinguished the soluble proteins in the tomato leaves infected by the compatible or incompatible strains. A protein of low molecular weight occurred only in the incompatible interaction. Some pathogenesis-related (PR) proteins, especially the 15, 18, 23, 26 and 54 kDa proteins, were detected only in the infected tomato leaves. In the two-dimensional electrophoresis, some proteins with different molecular weights (Mr. 21∼29 kDa) and the pI 8∼9 appeared more distinctly only in the incompatible interaction. These data suggest that the de novo synthesis of some PR proteins in tomato may be significant in defense against X. c. pv. vesicatoria.

• 한국자원식물학회지
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• 제30권3호
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• pp.311-317
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• 2017

This study was conducted with the aim of evaluating the antibiotic effects of leaves, stems, and roots in yacon (Smallanthus sonchifolius). Antibacterial activity of leaf extract by disk diffusion method with Bacillus subtilis and Escherichia coli respectively showed 13.3 and 13.75mm diameters of clear zone. There was no significant difference between the stems and leaves. The minimum inhibitory concentration of leaves' heating and agitation extraction showed a restrain of strain at 1mg/ml, but the stems and root extract did not appear. Yacon is a functional antibacterial material, and methanol extraction is more effective than water. This research was to investigate the growth stage of collection has the most effective antibacterial effects. It has collected yacon's leaves from June to October, which is an appropriate time for collection right before reaping. Yacon leaf has antibacterial effects on Bacillus subtilis, Escherichia coli, Enterococcus faecium, and Salmonella enteritifis. There were no significant differences by the growth stage of collection. Leaves collected in July are high in phenol which helps in sulfating activity works well considering the high scavenging capability of DPPH. Leaves collected in September are high in total flavonoid.

• Journal of Plant Biology
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• 제12권1호
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• pp.15-21
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• 1969

In this experiment, the litters of each five species of neadle-leaf trees and broad-leaf trees were laid on each pot soils, which had same soil conditions, is the green house and the sil fertility of each pots were determined after four years. Chemical properties among each pot soils under litters of needle-laef trees showed remarkable different values in the pH, base exchange capacity, total exchangeable base, base saturation, organic matter, available phosphorus, exchangeable potassium and calcium, and that of broad-leaf trees showed respectively significant difference. The content of chemical components, such as total exchangeable base, organic matter, total nitrogen, available phosphorus and exchanbeable potassium, between pot soils under litters of the needle-leaf trees and the broad-leaf trees were significant at the 0.01 and 0.05 levels of the statistical probability. The fertility of soil under the influence of decayed fallen leaves is the highest value in the posts of broad-leaves and next to the pots of needle-leaves and the control pots the lowest. The pH value of the soil with various kinds of fallen leaves showed little difference among themselves, but it especially approached in the broad leaves plots gradually to neutral and hte non-treated plot showed acidity. Lespedeza bicolar and Castanea crenata are supposed to contribute to the fertility of soil. Pinus rigida showed excellent exchanged properties of soi. The leaves of Robinia pseudoacacia and Pinus koraiensis did not contribute much to the promotion of fertility of soil.

• 식물분류학회지
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• 제37권4호
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• pp.351-361
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• 2007

In angiosperms, leaves typically develop as three-dimensional structure with dorsoventral, longitudinal, and lateral axes. We have shown that the control of two axes of leaves, longitudinal and lateral axis, can be genetically separable, and four classes of genes are responsible for the polar cell expansion and polar cell proliferation in Arabidopsis. In monocots, unifacial leaf, in which leaf surface consists only of abaxial identity, has been evolved in a number of divergent species. The unifacial leaves provide very unique opportunities for the developmental studies of the leaf axes formation in monocots, because their leaf polarities are highly disorganized. In addition, the mechanism of the parallel evolution of such drastic changes in leaf polarities is of interest from an evolutionary viewpoint. In this article, we describe our recent approaches to reveal the mechanism of unifacial leaf development and evolution, including recent advances in the leaf polarity specification in angiosperms.

• Journal of Plant Biology
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• 제31권3호
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• pp.205-215
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• 1988

The effects of simetryne on light induced electron transport and phosphorylation in isolated spinach (Spinacia oleracea L.) chloroplasts were investigated in comparison with sencor and DCMU. Simetryne, like sencor and DCMU, completely, inhibited PSII electron transport and phosphoryltion with 10-6 M treatment but did not inhibit PSI electron transport. Interference with the electron transport pathway was evidenced by the greater sensitivity of oxygen evolution and uptake than phosphorylation. The following order of decreasing inhibitory effectiveness was exihibited; DCMU>simetryne>sencor. The photoacoustic technique was also used to monitor the relative photosynthetic activity in the leaves treated with the herbicides (simetryne, sencor or DCMU) in vivo and in vitro. Photoacoustic measurements on intact leaves provide quantitative information on two related aspects of the photosynthetic process, namely, photochemical energy storage and oxygen evolution. The relative photoacoustic signal of leaves treated with the herbicides showed low level in 21 Hz, but high level in 380 Hz and on isolated chloroplasts (both 21 Hz and 380 Hz) in comparison with that of the untreated leaves. These results suggest that some of photochemical energy is converted into the heat owing to the inhibition of electorn transport pathway by the herbicides.

• Journal of Plant Biology
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• 제31권3호
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• pp.227-237
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• 1988

Effects of putrescine on senescence in detached leaves of Chinese cabbage (Brassica campestris L.) in the light were investigated. The putrescine as a potent antisenescence substane markedly inhibited chlorophyll and protein loss at the 10mM concentraton in the detached leaves during the dark incubation. In the light, however, putrescine showed the opposite effects to dark incubation. The chlorphyll loss by putrescine in the light was stopped with darktransfer, and inhibited competitively by a divalent cation Ca2+. In the light, putrescine reduced the protease activity. Putrescine, in the light, increased H2O2 content and reduced the activities of enzymes -superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6-involved in inhibiting the accumulation of free radicals. These results suggest that the effects of puterscine on chlorophyll and protein loss in detached leaves of Chinese cabbage in the light are related to the cationic nature of putrescine and the accumulation of free radicals.

• 한국식물병리학회지
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• 제11권1호
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• pp.61-65
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• 1995

Photosynthetic characteristics of soybean leaves infected with Pseudomonas syringae pv. glycinea were investigated for 8 days. The difference in photosynthesis rate between healthy and diseased soybean leaves decreased for 2 to 4 days after inoculation and then increased. In respiration rate, healthy and diseased leaves showed the same tendency as photosynthetic rate. The stomatal resistance increased following Pseudomonas syringae pv. glycinea infection. The total chlorophyll content of the infected leaf was less than that of the uninfected. Pseudomonas syringae pv. glycinea infection induced the malformation of stacked grana in chloroplast. Dry matter production declined after infection.

• 한국식물병리학회지
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• 제10권4호
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• pp.333-335
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• 1994

In 1991, the leaves and roots brown rot disease of scarlet kafir lily were found in Taejon and Seoul. The symptoms were appeared as dark-brown and water soaked on leaves. The discolored area of the leaves become halo. The roots revealed blight gray and water soaked. The pathogenic bacteria were isolated from the diseased leaves of the scarlet kafir lily were identified as Erwinia cypripedii on the bais of bacterial characteristics. E. cypripedii is first described bacteria which cause the disease on scarlet kafir lily in Korea. Therefore, we would like to propose to the name of scarlet kafir lily disease caused by E. cypripedii as“bacterial brown-rot of scarlet kafir lily”hereafter.

• 한국식물병리학회지
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• 제14권5호
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• pp.393-396
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• 1998

The characteristic Ramularia type conidia of Mycosphaerella nawae were formed on naturally infected leaves. Artificial inoculation with the conidia induced typical symptom on leaves, which was not distinguishable from those of ascospore infection, which has been considered as a primary inoculum source in nature. Also the morphology of the conidia produced on PDA was not different from those formed on artificially inoculated leaves or on naturally infected leaves at later stage of symptom development. Accordingly, we report the role of the conidia as a secondary inoculum of the circular leaf spot pathogen of persimmon for the first time.

• Journal of Plant Biology
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• 제9권3_4호
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• pp.14-18
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• 1966

1. The effects of saline and alkaline salts on the content of phosphorus in the leaves of selected vegetable plants under soil and sand culture was investigated. 2. The reduction of growth was associated with increasing intensity of salts, although no significant differences was evident. 3. Phosphorus content in the leaves of two plants was depressed with increasing concentration of two typs of salts. 4. It was noticed that the phosphorus content increased with treated salts. That is, phosphorus content was higher in the leaves of treated salt plots than that of control plots and the difference was significant in the soil culture. 5. The values of the phosphorus content were higher in the leaves of two crops of NaCl plots than that of $Na_2CO_3$ plots and the difference was significant in Radish.