• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.181-188
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• 2011

This study was carried out to estimate beneficial effects of Foeniculi fructus water extract on activities of key enzymes such as lipoprotein lipase (LPL), acyl-CoA synthetase (ACS), and hormone sensitive lipase (HSL) on lipid metabolism related with obesity. LPL and ACS were extracted from the epididymal adipose tissue and liver of C57BL/6J normal and obese mouse. Foeniculi fructus water extract treatment significantly reduced the activity of normal and obese LPL. When 100 ppm of Foeniculi fructus water extracts were tested, they decreased obese LPL activity by 12.0%. Foeniculi fructus water extract activated obese ACS activity by 7-fold compared with control at 1,000 ppm concentration. Expression of HSL mRNA was increased in Foeniculi fructus water extracts treated cells compared with non treated cells. All things considered, Foeniculi fructus water extract efficiently inhibits the influx of fatty acid into the cell, and activates metabolic process that uses fatty acids flowing as an energy source. Thus, it suggest that Foeniculi fructus water extract may have great potential as a novel anti-obesity agent.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.s01
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• pp.48-54
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• 1989

An immunoassay techniques using monoclonal antibodies have been developed of the quantitative analysis of endogenous hormones in plants. In order to be useful for immunoassay, both a high degree of specificity and a high affinity are required. A system is described for production of hybridomas which secrete antibodies against the plant hormone. Using the system we were able to produce hybridmas with the desired antibody specificity by cell fusion and culture method. For a number of obvious reasons, monoclonal antibodies(mAb) were superior to polyclonal antibodies.

• Magazine of the Korean Society of Agricultural Engineers
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• v.26 no.4
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• pp.66-71
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• 1984

In order to find out the effect of a plant growth regulating hormone, commonly called as 2, 4, 5-TP, for checking the resisting energy against falling of grain of Indica-Japonica species rice, an experiment was conducted with five levels of consistency (5,10, 15, 20, 25 ppm) and with four levels of spray timing (8, 17, 27, 34 DAH). Ten applications were made for measuring the resisting energy of rice heads sampled from every lot of treatments. Followings are the results; 1.The rice heads applied with 2, 4, 5-TP showed remarkably higher resisting energy against falling of grain comparing with those from the control plot. 2.The highest average value of the resisting energy was found as 83, 326 erg per grain on the plots of 8 DAH spray. 3.The general tendency was found that the more the timing of spraying was early, the more the consistency was lean. 4.A regression equation for expressing the peak resistance versus the timing was derived by means of least squares as follow; C=0. 00042D$^3$-0. 012D$^2$+0. 267D+8. 394 5.No significant differences were found among the values of weight per 1, 000 grains neither among moisture content.

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.157-157
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• 2023

Pre-harvest sprouting (PHS) in rice (Oryza sativa L.) is one of the main problems associated with seed dormancy. PHS causes yield loss and reduction of grain quality under unpredictable humid conditions at the ripening stage, thus affecting the economic value of the rice crop. To resolve this issue, it is important to understand the molecular mechanism underlying seed dormancy in rice. Recent studies have shown that seed dormancy is affected by a large number of genes associated with plant hormones. However, the effect of heat stress on seed dormancy and plant hormones is not well understood. In this study, we compared the PHS rate as well as the transcriptome and small RNAome of the seed embryo and endosperm of two different accessions of rice, PHS-susceptible rice (low dormancy) and PHS-resistant rice (high dormancy) under three different maturation stages. We identified and verified the candidate genes associated with seed dormancy and heat stress-related responses in rice using quantitative real-time PCR. We newly discovered hormone-related genes, heat shock protein-related genes, and miRNAs potentially involved in PHS. These findings provide a foundation for understanding the dynamics of transcriptome and small RNAome of hormone- and heat stress-related genes, which affect PHS during seed maturation.

• FLOWER RESEARCH JOURNAL
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• v.26 no.4
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• pp.179-186
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• 2018

Ranunculus asiaticus characterizes colorful and attractive flower shapes that are related with the ornamental value of bulbous plants. Improving ornamental value of bulbous flowers has been the general goal of floricultural market. Gibberellic acid ($GA_3$) and benzyladenine (BA) play an important role in growth and developmental processes in floriculture. Combinational treatments of these two hormones have been used in floriculture to improve flower quality. We assessed the effects of combined $GA_3$ and BA, as well as the individual effects of each hormone, on growth characteristics using soil drench application to eight R. asiaticus cultivars, 'Giallo Millepetali', 'Bianco Millepetali', 'Arancio Millepetali', 'Rosa SC', 'Arancio Pratolino', 'Giallo Pratolino', 'Bianco Pratolino', and 'Rosa Ch Pratolino'. $GA_3$ treatments increased plant height and first flower size of R. asiaticus cultivars. Moreover, about 5 to 9 days to flowering were averagely shortened by $GA_3$ treatments compared to controls. On the other hand, the opposites, including first flower size and days to flowering, were observed for cultivars treated with BA, compared with controls. Treatments of $GA_3$ + BA generally affected growth traits, such as plant height, flower size, and the timing of flowering on some R. asiaticus cultivars. In particular, about 5 to 6 days to flowering were reduced on average by Treatments of $GA_3$ + BA. Our results showed positive growth effects, including plant height, days to flowering, first flower height, number of flowers from the application of individual and combined hormones to R. asiaticus cultivars and demonstrate a role for these hormones in future bulbous floriculture.

• Journal of People, Plants, and Environment
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• v.22 no.4
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• pp.333-340
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• 2019

This study was conducted to find out the effects of plant cultivation activities in the village gardening program hosted by Gyeonggi-do on the psychology and physiology of participants. This study conducted a survey on 20 villagers aged 57.70±9.23 on average and collected their saliva before Session 1 and after the final Session 10. The experiment was conducted by carrying out the village gardening program once a week for total 10 weeks. Psychological assessment was conducted by measuring the level of plant cultivation experience, four elements (repose, fascination, coherence, legibility) of the Perceived Restorativeness Scale (PRS) and stress levels, and also conducting the Wilcoxon paired signed ranks test. As the result of comparatively analyzing the saliva collected before and after the program, the total cortisol level, which is a stress hormone, decreased with statistical significance after the program (t=14.521, p<.001). The level of plant cultivation activities increased with statistical significance after the program, and the PRS increased with statistical significance in the three elements of repose, fascination and legibility excluding coherence. In addition, as the result of examining the change in the stress level of participants with the sum of all item scores, it was found that the stress level decreased with statistical significance (p<.05) from 17.65 points to 15.30 points after the program. Hence, plant cultivation activities in the village community have positive effects such as attention restoration or stress relief. In addition, the activities can also contribute to mental connection and healthcare of residents.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.129-134
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• 2005

Callus induction from a leaf explant has been achieved in Lilium Oriental hybrid 'Casa Blanca'. The highest frequency of callus induction was obtained on MS medium supplemented with 0.5 mg/L BA and 2.0 mg/L NAA after 2 months of culture. The cultures maintained continuously without change in color and type of callus when they cultured in the dark. Plantlet regeneration with a high frequency was achieved from induced calli on the same medium. A number of shoots are formed from one cluster of callus, and bulblets developed into intact plantlets after transfer to hormone-free MS medium. No phenotypic variations were observed among regenerants. Enhancement in plantlet regeneration via callus formation would be expected to facilitate the efficiency of transformation of this Oriental hybrid 'Casa Blanca'.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.281-292
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• 2016

Plants can recognize and respond in various ways to diverse environmental stresses, including pathogenic microorganisms, salt, drought, and low temperature. Salicylic acid (SA) is one phytohormone that plays important roles in the regulation of plant growth and development. Nonexpressor of pathogenesis-related genes 1 (NPR1) was originally identified as a core protein that could function as a transcriptional co-regulator and SA receptor during systemic acquired resistance (SAR), a plant immune response that could activate PR genes after pre-exposure of a pathogen. Although the function of NPR1 in plant defense response and the role of SA hormone in the regulation of plant physiological processes have been well characterized, the biological role of NPR1 in plant abiotic stress responses is largely unknown. In this review, we will summarize and discuss the current understanding of NPR1 function in response to plant environmental stresses.

• Journal of Life Science
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• v.8 no.6
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• pp.623-626
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• 1998

Conditions for somatic embryogenesis and plant regeneration from stem tissues of Euphorbia pulcherrima were esta-blished. Explants from leaf, petiole, stem were examined for their embryogenesis on MS solid medium supplemented with plant growth hormones in combination at various concentrations. From leaf or petiole explants, callus was indu-ced well but never proceeded to the embryonic stage in our expermental conditions. From stem explants, however, multiple shoots following callus induction emerged in about 6 to 8 weeks on MS agar medium supplemented with 1.5 mg/L of benzyladenine. Upon transfer, roots were developed on hormone-free MS solid medium.

• Journal of Plant Biology
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• v.37 no.3
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• pp.333-341
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• 1994

Effective plant regeneration from immature cotyledons of soybean [Glycine max (L.) Merr.] cultivars was achieved via somatic embryogenesis. Somatic embryogenesis was performed with the cotyledons of immature embryos 14-20 d after flowering. Immature cotyledons of cv. Whangkeum were placed abaxial or adaxial side down on modified MS medium containing 20mg/L 2,4-D. The greatest number of somatic embryos, 1.2 per cotyledon, was produced from those of 4.0-4.9 mm in length which had been placed abaxial side down. Among cvs. Pecking, Whangkeum and Baekwoon, Pecking had the highest embryo induction efficiency with 4.3 somatic embryos per cotyledon in 20mg/L 2,4-D treatment and with 1.0 embryo per cotyledon in 8mg/L NAA treatment. Germinable globular somatic embryos were induced with the highest efficiency, 27.6%, in 20mg/L 2,4-D and were proliferated efficiently on liquid medium containing 10mg/L 2,4-D. The globular somatic embryos developed into germinable mature somatic embryos on medium containing 10 $\mu$M CoCl2, 9% sucrose, and 0.5% activated charcoal. These mature somatic embryos germinated on hormone-free mediu. After transfer to the soil, regenerated plants with seeds were obtained.