• 한국미생물·생명공학회지
• /
• 제49권3호
• /
• pp.374-383
• /
• 2021

Rhizoctonia solani is one of the major pathogens that cause sheath blight disease in rice. Sheath blight is one of the most difficult diseases to control. Biological control (with the use of rhizobacteria) is one of the ways to control this disease. Plant Growth Promoting Rhizobacteria (PGPR) is a rhizosphere bacterium that can be used to enhance plant growth. The composition of the rhizobacteria in organic and nonorganic soil is affected by the chemical characteristics of the soil - which influences plant physiology and root exudation patterns. This study aimed to obtain a species of rhizobacteria which shows PGPR activity, from organic and nonorganic rice fields and test their capability to suppress R. solani growth. Out of 23 isolates screened for PGPR activity, the following isolates showed high PGPR activity and were selected for in vitro antagonistic activity testing against R. solani: ISO6, ISO11, ISO15, ISN2, ISN3, and ISN7, The six isolates produced 43,42-75,23 ppm of IAA, possessed phosphorus solubilization capability, and chitinase-producing activity. ISO6 (54.88%) and ISN7 (83.33%) displayed high inhibition capacities against R. solani, in vitro. ISO6 and ISN7 inhibited the growth of R. solani lesions on rice leaves by 89% and 100% (without lesion), respectively, after 7 days of incubation. Analysis of their 16S rRNA sequences revealed that the ISO6 isolate was Citrobacter freundii and ISN7 isolate was Pseudomonas aeruginosa.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2003년도 춘계학술대회
• /
• pp.643-647
• /
• 2003

Power plant Piping operating at elevated temperature often fails prematurely by the growth of microcracks under creep conditions. Therefore, the life assessment of high temperature components that contain cracks is an important technological problem. The mechanisms of crack growth in simple metals and alloys have been investigated using both mechanical and microstructural approaches. In this study, life prediction accounting for creep, crack growth and thermal stress is analyzed.

• 분석과학
• /
• 제35권4호
• /
• pp.181-188
• /
• 2022

This paper describes a comparative analysis of yeast cell viability at exponential and stationary growth phases using multiple conventional techniques and statistical tools. Overall, cellular responses to various viability assays were asynchronous. Results of optical density measurement and direct cell counting were asynchronous both at exponential and stationary phases. Proliferative capacity measurement using SP-SDS indicated that cells at the end of the stationary phase were proliferative as much as exponentially growing cells. Metabolic activity assays using two different dyes concluded that the inside of cells at stationary phase is slightly less reducing compared to that of exponentially growing cells, implying that the metabolic activity imperceptibly declined as cells were aged. These results will be helpful to understand the details of yeast cell viability at exponential and stationary growth phases.

• 산업경영시스템학회지
• /
• 제43권2호
• /
• pp.65-71
• /
• 2020

Worldwide plant market keeps maintaining steady growth rate and along with this trend, domestic plant market and its contractors also maintain such growing tendency. However, in spite of its external growth, win-win growth of domestic material industry that occupies the biggest share in plant industry cost portion is extremely marginal in reality. Domestic plant material suppliers are required to increase awareness of domestic material brand by securing quality and reliability of international standard through improvement of design quality superior to that of overseas material suppliers. Improvement of design quality of plant material becomes an essential element, not an option, for survival of domestic plant industry and its suppliers. Under this background, in this study, priority and importance by each evaluation index was analyzed by materializing plant design stage through survey of experts and defining evaluation index by each design stage and based on this analysis result, evaluation index of stage-gate based decision-making process that may improve design quality of plant material was suggested. It is considered that by utilizing evaluation index of stage-gate based decision-making process being suggested in this study, effective and efficient decision-making of project decision-makers would be enabled and it would be contributory to improve design quality of plant material.

• Journal of Microbiology and Biotechnology
• /
• 제12권2호
• /
• pp.249-257
• /
• 2002

Pseudomonas fluorescens GL20 is a plant growth-promoting rhizobacterium that produces a large amount of hydroxamate siderophore under iron-limited conditions. The strain GL20 considerably inhibited the spore germination and hyphal growth of a plant pathogenic fungus, Fusarium solani, when iron was limited, significantly suppressed the root-rot disease on beans caused by F. solani, and enhanced the plant growth. The mechanism for the beneficial effect of strain GL20 on the disease suppression was due to the siderophore production, evidenced by mutant strains derived from the strain. Analysis of the outer membrane protein profile revealed that the growth of strain GL20 induced the synthesis of specific iron-regulated outer membrane proteins with molecular masses of 85- and 90 kDa as the high-affinity receptors for the ferric siderophore. In addition, a cross-feeding assay revealed the presence of multiple inducible receptors for heterologous siderophores in the strain. In order to induce increased efficacy and potential in biological control of plant disease, a siderophore-overproducing mutant, GL20-S207, was prepared by NTG mutagenesis. The mutant GL20-S207 produced nearly 2.3 times more siderophore than the parent strain. In pot trials of beans with F. solani, the mutant increased plant growth up to 1.5 times compared with that of the parent strain. These results suggest that the plant growth-promoting P. fluorescens GL20 and the genetically bred P. fluorescens GL20-S207 can play an important role in the biological control of soil-borne plant diseases in the rhizosphere.

• 원예과학기술지
• /
• 제29권3호
• /
• pp.201-210
• /
• 2011

This experiment was conducted to investigate physical and chemical characteristics by volume fractions of root media using peatmoss, perlite, and vermiculite, along with effects on the growth of pot chrysanthemums (Dendranthema ${\times}$ grandiflorum 'Vemini') in a C-channel mat irrigation system. To evaluate the physico-chemical properties of 20 root media, the bulk density, particle density, total pore space, pore space, ash content, organic matter, pH, and electrical conductivity were measured and data were analyzed using principal component analysis (PCA). PCA scores revealed that physico-chemical properties changed by the blending of peatmoss, perlite, and vermiculite. The 20 root media were divided into three main groups by hierarchical cluster analysis. At the end of the experiment, the pH and EC of the root media were measured from media divided into four layers. The pH of root media without plants showed a strong linear relationship and the pH of root media with plants increased exponentially. The change of EC in the root medium was indicated as a hyperbolic curve. Plant growth characteristics according to growth in the 20 root media were analyzed by PCA. It was found that the mixing ratios of the root media affected plant growth characteristics. Therefore, mixing ratio is an important factor for pot-plant production in a subirrigation system.

• 한국농업기계학회:학술대회논문집
• /
• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
• /
• pp.765-772
• /
• 1996

Objectives of this study were to develop an application method of a numerical retina using the finite element model and to investigate the performance of image features extraction in comparison to the textural analysis. Using a plant community of radish sprouts, excellent resolution of the finite element retina was revealed. The sensitivity analysis of the finite element retina from engineering point of view was discussed. The importance of sensitivity analysis of the finite element retina was pointed out in terms of extraction of effective image features of plant community . Technical details of maximizing the sensitivity of the finite element retina to populated plant canopy were also discussed.

• 한국환경농학회지
• /
• 제30권4호
• /
• pp.395-401
• /
• 2011

BACKGROUND: Potassium (K) is one of the macronutrients which are essential for plant growth and development. Its deficiency in paddy soils is becoming one of the limiting factors for increasing rice yield in Asia. METHODS AND RESULTS: To investigate physiological symptoms under K-starvation (NP) compared with complete media (NPK) condition, we measured shoot/root length, weight, nutrients, and patterns of protein expression. The shoot growth was significantly reduced, but root growth was not affected by K-starvation. However, biomasses were decreased in both shoot and root. Uptake of K was reduced up to 85%, while total concentrations of P, Ca, Mg, Na were increased in root and shoot. To better understand the starved K mechanism of rice, comparative proteome analysis for proteins isolated from rice leaves was conducted using 2-DGE. Five spots of differentially expressed proteins were analyzed by MALDI-TOF MS. Analysis of these K-starvation response proteins suggested that they were involved in metabolism and defense. CONCLUSION(s): Physiological and 2-DGE based proteomics approach used in our study results in observation of morphology or nutrients change and identification of K-starvation responsive proteins in rice root. These results have important roles in maintaining nutrient homeostasis and would also be useful for further characterization of protein function in plant K nutrition.

• 환경생물
• /
• 제34권3호
• /
• pp.161-168
• /
• 2016

본 연구에서는 엽록소 형광반응 (OJIP)분석을 통해 고추재배에서 폐달걀 분해 액비의 생육증진효과에 대하여 평가하였다. 고추재배 전기간을 통하여 고추의 생장은 통계학적으로 유의성을 보이지는 않았으나, 폐달걀 분해 액비를 처리한 실험구에서 좋은 생육을 보였다. 엽록소 형광반응 실험 시작한 후 무처리구의 엽록소 형광량이 분해 액비의 형광량보다 커서 분해 액비 처리구의 광이용 효율이 더 좋았음을 알 수 있었다. 이는 결국 광합성량의 증가로 이어질 것으로 판단된다. 광화학 매개변수 중 Fo, ABS/RC, RC/ABS, TRo/RC, $DI_0$/RC, $\text{DF_{TOTAL ABS}}$ 등 6개 parameter가 광화학 반응의 효율을 나타내는 중요한 요인으로 판단되었다.

• Journal of Plant Biotechnology
• /
• 제47권4호
• /
• pp.337-344
• /
• 2020

식물생장촉진 미생물은 식물 뿌리에 영양을 원활하게 공급하거나 휘발성 유기화합물(Volatile Organic Compound, VOC)를 이용하여 식물의 내재 인자와 상호작용을 통해 생장을 촉진한다. 본 연구에서는 식물 생장 촉진 진균 UOS의 담배에서의 생장 촉진 효과를 평가하고, 계통발생학적 분석을 통해 동정하였다. 또한, UOS의 식물 생장 촉진 인자를 탐색하고자 Gas Chromatography-Mass Spectrometry (GC-MS) 분석을 통해 UOS의 VOCs를 확인하였다. UOS를 처리한 담배는 무처리구에 비해서 3.8배의 생중량이 증가하였고, I-plate를 이용한 분리된 공간에서는 UOS처리구가 무처리에 비해 생중량이 4.2배 증가하였다. 또한, UOS 처리구의 식물은 주근의 길이가 약2배 짧아지고 측근의 수가 약2배 증가하였다. UOS은 포자 및 균사 형태와 Internal transcribed spacer (ITS) 유전자 염기서열을 통하여 Phoma sp.으로 동정되었으며, 이들은 GC-MS분석을 통해 UOS는 hexamethylcyclotrisiloxane (D3)라는 VOC를 갖고 있는 것이 밝혀졌다. 이 결과들은 Phoma sp.의 진균 UOS가 VOC물질인 D3을 통해서 간접적으로 식물 생장 촉진 및 뿌리 발달에 영향을 미치는 것을 나타낸다. UOS와 그의 VOC인 D3의 활용은 농업에서 생장량 증대에 기여할 것으로 판단된다.